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Xylan-Degrading Enzymes: Application Prospects Ifendu Nnanna, Ph.D. AgResources Development Group, LLC http://www.adgintl.com
[object Object],OBJECTIVE
[object Object],[object Object],WHAT THEY DO Xylan-degrading enzymes belong to the family of glycosyl-hydrolases (glycosidases)
Glycosidases play important roles in  polysaccharide hydrolysis ,[object Object],[object Object],[object Object],[object Object],[object Object],Ref:  R. V. Wolfenden, Chemical & Engineering News, July 6, 1998  WHAT THEY DO
XYLAN-DEGRADING ENZYMES Enzyme Type     EC Code  Synonym   Main chain cleaving enzymes 1,4-  -D-xylan xylanohydrolase  EC 3.2.1.8  Endoxylanase 1,4-  -D-xylan xylohydrolase  EC 3.2.1.37    -xylosidase (exo) Side chain cleaving enzymes  -L-arabinofuranosidase   EC 3.2.1.55  Arabinosidase Acetyl esterase  EC 3.1.1.6  Acetyl esterase Acteyl xylan esterase  (?)  Acetyl xylan esterase  -(4-O-methyl)-D-glucuronidase  EC 3.2.1.    -glucuronidase Feruloyl esterase  (?)  Ferulic acid esterase
MODE OF ACTION  XYLAN-DEGRADING ENZYMES --4Xyl  1- 4Xyl  1- 4Xyl  1- 4Xyl  1- 4Xyl  1- 4Xyl  1- 4Xyl  1- 4Xyl  1- 4Xyl  1- 4Xyl  1- 4Xyl  1-- Ac=acetyl group; Xyl = xylose; Ara f  =L-arabinofuranose; MeGlcA = 4-O-methyl-D-glucuronic acid; FA = ferulic acid.   [ Adapted from Biely (1985), Trends in Biotechnol  3 : 286 ]  (a)  -L-arabinofuranosidase (b)   -glucuronidase 3 Ac 3 1  Ara f 3 1  Ara f 5 FA 2 Ac 2 1  MeGlcA Acetyl xylan esterase (b) Ferulic acid esterase Xyl  1- 4Xyl  1  -xylosidase Endoxylanase Acetyl xylan esterase (a)
There is a Multidisciplinary Interest in Xylan-Degrading Enzymes   ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],SIGNIFICANCE
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],SIGNIFICANCE The Industrial Impact and Potential Applications of xylan-degrading enzymes are broad.
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],APPLICATION   OPPORTUNITIES Xylan, the main hemicellulose in plant cell walls, is the most abundant renewable polysaccharide in nature after cellulose.
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],APPLICATION   OPPORTUNITIES Each year, agricultural processing industries generate billions of pounds of fibrous waste, by-products that are of low value or pose environmental problems.  Examples.....
[object Object],[object Object],[object Object],[object Object],[object Object],SUMMARY http://www.adgintl.com

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Xylan-Degrading Enzymes: Application Prospects

  • 1. Xylan-Degrading Enzymes: Application Prospects Ifendu Nnanna, Ph.D. AgResources Development Group, LLC http://www.adgintl.com
  • 2.
  • 3.
  • 4.
  • 5. XYLAN-DEGRADING ENZYMES Enzyme Type EC Code Synonym Main chain cleaving enzymes 1,4-  -D-xylan xylanohydrolase EC 3.2.1.8 Endoxylanase 1,4-  -D-xylan xylohydrolase EC 3.2.1.37  -xylosidase (exo) Side chain cleaving enzymes  -L-arabinofuranosidase EC 3.2.1.55 Arabinosidase Acetyl esterase EC 3.1.1.6 Acetyl esterase Acteyl xylan esterase (?) Acetyl xylan esterase  -(4-O-methyl)-D-glucuronidase EC 3.2.1.  -glucuronidase Feruloyl esterase (?) Ferulic acid esterase
  • 6. MODE OF ACTION XYLAN-DEGRADING ENZYMES --4Xyl  1- 4Xyl  1- 4Xyl  1- 4Xyl  1- 4Xyl  1- 4Xyl  1- 4Xyl  1- 4Xyl  1- 4Xyl  1- 4Xyl  1- 4Xyl  1-- Ac=acetyl group; Xyl = xylose; Ara f =L-arabinofuranose; MeGlcA = 4-O-methyl-D-glucuronic acid; FA = ferulic acid. [ Adapted from Biely (1985), Trends in Biotechnol 3 : 286 ]  (a)  -L-arabinofuranosidase (b)  -glucuronidase 3 Ac 3 1  Ara f 3 1  Ara f 5 FA 2 Ac 2 1  MeGlcA Acetyl xylan esterase (b) Ferulic acid esterase Xyl  1- 4Xyl  1  -xylosidase Endoxylanase Acetyl xylan esterase (a)
  • 7.
  • 8.
  • 9.
  • 10.
  • 11.