Dr. Lenkinski will discuss the utility of this system for cancer research, and will spend some time discussing his imaging techniques for both multi-parametric multi-modality imaging, along with showing examples of how he configured his imaging facility to perform sequential PET/MRI studies. Validation examples from patient derived xenograft an implanted cell line models will be shown, and finally Dr. Lenkinski will finish by showing some examples of perfusion studies. In this free webinar hosted by Scintica Instrumentation Dr. Lenkinski will review some of the key research studies he performed using the M-seriesTM compact MRI systems from Aspect Imaging at both UT Southwestern and Harvard Medical Centers. These systems remove barriers for the preclinical researcher, allowing them access to an imaging modality once thought to be attainable to only a handful of specialized researchers. These systems are cost effective, both in purchase price and running and maintenance costs; they require no specialized infrastructure, plumbing for cooling water, or cryogens; and they have been designed to be simple to operate, requiring no previous background in MR physics. Topics discussed in this webinar included: Monitoring tumor growth non-invasively MRI in multi parametric multimodality imaging – examples Examples of sequential PET/MRI Validation of multimodality approaches in clinical studies – PDX models and implanted cell line models Perfusion studies If you want more information on our small animal MRI options visit our Aspect Imaging page www.scintica.com

1. Robert E. Lenkinski
Adjunct Professor
Department of Radiology
Scintica Instrumentation
Phone: +1 (519) 914 5495
sales@scintica.com
Potential Roles for the
Aspect 1T M2 Preclinical
Scanner in Imaging
Research

2. Potential Roles for the Aspect 1T
M2 Preclinical Scanner in Imaging Research
Robert E. Lenkinski
Adjunct Professor
Department of Radiology

3. Outline
USE CASES
1. Monitoring Tumor growth non-invasively
2. MRI in Multi-parametric Multi-modality examples of
sequential PET/MRI
3. Validation of Multimodality approaches in clinical
studies-PDX models and implanted cell line models
4. Perfusion studies

4. http://www.longwoodsaif.org/

5. 8.5 Tesla / 9 cm bore
4.7 Tesla / 30 cm bore
MRI Magnets (Superconducting)
for Animal Scanning

6. Trends
MRI is widely used as a modaliy for imaging
mouse models of cancer
There are an increasing number of studies using
Dual-modality and Multi-modality imaging

7. Siting A Superconducting High Field System

8. Safety Issues with Fringe Fields

9. There Are Practical Issues : Animals!

10. A. Protocols / Housing / In and Out

11. So why do we need a Compact
MRI?
1. Regulatory restrictions
associated with radionuclides.
2. Co-registration.
3. Safety (no-fringe field) and
convenience.

12. http://www.longwoodsaif.org/

13. Conventional T2W SE 2500/100
Excellent visualization
of Zonal architecture in
the mouse kidney
Minimal Motion
Arifacts

14. FSE 4500/120

15. Proximity to PET and SPECT
Systems

16. Common Mouse Bed

17. T1 SE Multislice 2010 Nov 03
Scan # 3295 (Axial from
3289)
Scan Dimensions 2D
Orientation Axial
3D encodings 1
# excited slices 15
Slice thickness 1
Interslice space 1
FOV 100
Slice position 6
Freq direction Horizontal
DICOM n_slices 40
Phi Angle 0
Theta Angle 0
Echo Time 11.4
Repetition Time 400
Flip Angle 90
Samples 400
Encodings 400
Excitations 8
Echo Asymmetry 0
Dwell Time 16
Slice Interleave mode INTRLV
Gain calibration
options
ALWAYS

18. Glucose and FDG metabolism.
Hoffman J M , Gambhir S S Radiology 2007;244:39-47
©2007 by Radiological Society of North America

19. Intrascapular brown fat
MRI FDG-PET PET/MRI
Purpose: to demonstrate co-registration of PET/MRI -- proper
orientation and scaling.

20. PET/MRI--- Intrascapular brown fat
MRI PET PET/MRI
Heart also shown (blue arrow)

22. Mouse Prostate Anatomy
Abbott et al. Genome Biology 2003 4:R79

23. Genetic Mouse Model (PTEN) of Spontaneous Prostate Cancer
MRI PET PET/MRI
Akash Patnaik
Lewis Cantley
John V. Frangioni
Robert E. Lenkinski
Elaine Lunsford
Kyle Robichaud

24. http://www.invivoscope.com/
Image analysis software developed for Bioscan systems (Mediso)
by InVICRO http://www.invicro.com/.
We use this package to co-register and fuse the Aspect MRI and
Bioscan PET images.

25. While we switch between our speakers, tell us
more about your research
• What animal models are you working with,
• What types of instruments do you work with,
and
• What are your research goals?
Please type your answers in the Q&A box below:

26. Preclinical Multi-Modality Imaging
Example:-PET/MRI of Lung Cancer Models
Investigators: Ralph Deberardinis, Chis Hensley
Protocol
1. Anesthetize the mouse and place the mouse on common mouse holder.
2. Inject the mouse with FDG (wait 60 minutes for best tumor
signal/background).
3. Image the mouse on the Aspect MR system-anatomical imaging followed
by DCEMRI and DWI.
4. Transfer the mouse using the common bed and anesthesia apparatus to
the MicroPET/CT and acquire the FDG images.
5. Register and fuse the PET/MRI on a workstation (there are several
available including VivoQuant).

27. The mouse is anesthetized and placed on common bed
The next slides pictorially illustrate our PET/MRI protocol

28. Inject the mouse with FDG for MicroPET/CT

29. The mouse is placed into the Aspect 1.0T MRI for imaging

30. The mouse is transferred to the MicroPET for FDG imaging

31. FDG Images and Surface plots (Left) and DCEMRI Images and Surface plots (right)

32. Cited 327 times
Cited 230 times

33. PET tracers can inform intratumoral
heterogeneity sampling in mouse models
Tumor
FDG–glucose uptake
LTL-613: Squamous PDX
Saleh Ramezani

34. This inverse correlation between glucose metabolism and
perfusion is upheld in cell line flank xenografts
Gedaa Hassan & Keith Hulsey

35. This phenomenon is replicated with the
hypoxia marker F-MISO
• FDG and F-MISO correlate
in clinical studies.
Tumor
A549: Adenocarcinoma
Cell Line
Guiyang Hao Saleh Ramezani

36. Audience Poll

37. What Does the Compact Aspect Do for
PET/MRI
It is difficult, If not impossible, to interpret the PET
without the MRI and vice versa.
Regulatory compliant.
Provides simple, reproducible, robust, co-
registration and fusion.
Simplifies workflow.

38. Perfusion Studies on M2

39. Flow-Sensitive Alternating Inversion Recovery (FAIR)

41. What Does the Compact Aspect Do for Us
Provides 1.0T image quality with an easy to use
user interface.
Very easy and safe to site (small footprint; no
fringe field).
Enables compliant, easy to use, safe, convenient,
multimodality imaging.

42. To ask a question, click the Q&A Button,
type your question and click send. Any
questions that are not addressed during
the live webinar will be answered
following the event.
Please indicate whom you want to
address your question.
Thank you for participating!
Q&A
SESSION:
Robert E. Lenkinski
Adjunct Professor
Department of Radiology