Economics of animal health: A little theory and some applicationsHenk Hogeveen
At the moment I am in Kenya, at a site visit of Dr Esther Wafula, who is doing a PhD with us in Utrecht. Today I gave a presentation at KARI, the Kenyan Agricultural Research Institute. This is a huge institute, consisting of more than 3000 people. People responsible for animal health work both from KARI as well as the University of Nairobi were present.
The presentation has quite some overlap with the one I gave last year in Kupang. However, I added some new figures about the complexity of management as well as the first research results of Dr Wafula: costs of trypanosomosis.
Anaesthetic emergencies and procedures in veterinary practicesIVRI
This document discusses various anesthetic emergencies and procedures. It covers topics like cardiovascular emergencies, hemorrhage, cardiac arrest, allergic reactions, and cardiopulmonary resuscitation. For cardiac arrest, it recommends following the ABCD protocol of securing the airway, providing breathing support, performing cardiac compressions, and administering epinephrine and other drugs. It describes performing external chest compressions initially, but notes internal cardiac massage is more effective for resuscitation after 2 minutes if there is no response.
This document discusses the use of herbal immunomodulators in poultry to improve immunity and combat antibiotic resistance. It provides background on immunomodulators and how certain herbs like ashwagandha, neem, tinospora cordifolia, and mint can modulate the immune system through effects on cytokines, immune cells, and phagocytosis. Several studies are summarized that show herbs like ashwagandha and tinospora cordifolia can improve performance parameters and immune responses in broiler chickens and quails. The document concludes that herbs have potential immunomodulatory properties through effects on macrophages, cytokines, and immune cells like NK cells.
El documento resume la fisiología del ciclo estral en ganado bovino, incluyendo el desarrollo folicular, la ovulación, la formación y regresión del cuerpo lúteo, y los cambios hormonales asociados. También describe protocolos de inseminación artificial a tiempo fijo y su efecto en los índices reproductivos en comparación con la inseminación artificial tradicional. Finalmente, analiza técnicas para mejorar la detección de celos y la ciclicidad posparto que aumentan la eficiencia de los programas
This document discusses fluid therapy in animals. It begins by describing the distribution of water in the body and the composition of intracellular and extracellular fluids. It then discusses three types of fluid disturbances: changes in volume, content, and distribution. The document outlines different types of fluid therapy including replacement, adjunctive, and supportive therapies. It provides details on routes of fluid administration and indications for intravenous fluids. Throughout, it discusses evaluating and monitoring fluid therapy, diagnosing and treating dehydration, and complications of intravenous fluids.
There are four main types of mange in dogs: demodectic mange caused by Demodex mites; sarcoptic mange caused by Sarcoptes mites, which is highly contagious and zoonotic; chyletiolosis caused by Cheyletialla mites, which is also contagious and zoonotic; and ear mites caused by Oteodectis mites, which can also be transmitted to humans. Mange is transmitted through close contact between dogs and causes symptoms like itching, rashes, and scratching that can lead to secondary infections. Treatment involves topical acaricides and creams or oral medications like ivermectin.
Economics of animal health: A little theory and some applicationsHenk Hogeveen
At the moment I am in Kenya, at a site visit of Dr Esther Wafula, who is doing a PhD with us in Utrecht. Today I gave a presentation at KARI, the Kenyan Agricultural Research Institute. This is a huge institute, consisting of more than 3000 people. People responsible for animal health work both from KARI as well as the University of Nairobi were present.
The presentation has quite some overlap with the one I gave last year in Kupang. However, I added some new figures about the complexity of management as well as the first research results of Dr Wafula: costs of trypanosomosis.
Anaesthetic emergencies and procedures in veterinary practicesIVRI
This document discusses various anesthetic emergencies and procedures. It covers topics like cardiovascular emergencies, hemorrhage, cardiac arrest, allergic reactions, and cardiopulmonary resuscitation. For cardiac arrest, it recommends following the ABCD protocol of securing the airway, providing breathing support, performing cardiac compressions, and administering epinephrine and other drugs. It describes performing external chest compressions initially, but notes internal cardiac massage is more effective for resuscitation after 2 minutes if there is no response.
This document discusses the use of herbal immunomodulators in poultry to improve immunity and combat antibiotic resistance. It provides background on immunomodulators and how certain herbs like ashwagandha, neem, tinospora cordifolia, and mint can modulate the immune system through effects on cytokines, immune cells, and phagocytosis. Several studies are summarized that show herbs like ashwagandha and tinospora cordifolia can improve performance parameters and immune responses in broiler chickens and quails. The document concludes that herbs have potential immunomodulatory properties through effects on macrophages, cytokines, and immune cells like NK cells.
El documento resume la fisiología del ciclo estral en ganado bovino, incluyendo el desarrollo folicular, la ovulación, la formación y regresión del cuerpo lúteo, y los cambios hormonales asociados. También describe protocolos de inseminación artificial a tiempo fijo y su efecto en los índices reproductivos en comparación con la inseminación artificial tradicional. Finalmente, analiza técnicas para mejorar la detección de celos y la ciclicidad posparto que aumentan la eficiencia de los programas
This document discusses fluid therapy in animals. It begins by describing the distribution of water in the body and the composition of intracellular and extracellular fluids. It then discusses three types of fluid disturbances: changes in volume, content, and distribution. The document outlines different types of fluid therapy including replacement, adjunctive, and supportive therapies. It provides details on routes of fluid administration and indications for intravenous fluids. Throughout, it discusses evaluating and monitoring fluid therapy, diagnosing and treating dehydration, and complications of intravenous fluids.
There are four main types of mange in dogs: demodectic mange caused by Demodex mites; sarcoptic mange caused by Sarcoptes mites, which is highly contagious and zoonotic; chyletiolosis caused by Cheyletialla mites, which is also contagious and zoonotic; and ear mites caused by Oteodectis mites, which can also be transmitted to humans. Mange is transmitted through close contact between dogs and causes symptoms like itching, rashes, and scratching that can lead to secondary infections. Treatment involves topical acaricides and creams or oral medications like ivermectin.
Dysregulation of macrophage signal transduction by ToxoplasmaIshfaq Maqbool
A brief account of mechanism adopted by Toxoplasma gondii to evade the immune response of the host immune cells particularly macrophages by disruption of macrophage signal transduction
This document discusses the host protective roles of type 2 immunity in response to parasitic infections. It summarizes that type 2 immunity involves both innate and adaptive immune cells that work together to kill parasites and repair tissue damage through mechanisms like alternate macrophage activation. Key cells involved include ILC2s, eosinophils, mast cells, and alternatively activated macrophages that secrete molecules like IL-4, IL-5, IL-13, and arginase to expel parasites and promote wound healing.
Immunity against Helminths:role of InterleukinsIshfaq Maqbool
The document summarizes key aspects of the immune response against helminth parasites. It notes that helminths typically induce a type 2 immune response characterized by cytokines like IL-4, IL-5, and IL-13. This non-inflammatory response involves alternatively activated macrophages, eosinophils, and other effector cells that work to expel and kill parasites while repairing tissue damage. The response differs from bacterial and viral immunity, with Th1 responses only occurring during early larval migration stages.
Autophagy plays multifaceted roles in both the innate and adaptive immune system. In innate immunity, autophagy aids in the recognition of pathogens by pattern recognition receptors like TLRs, NLRs, and RLRs. It also facilitates the handling and destruction of intracellular bacteria by phagocytosis and lysosomal fusion. Autophagy regulates the production of cytokines as well. In adaptive immunity, autophagy contributes to antigen presentation on MHC class I and II molecules and is important for the homeostasis and functions of lymphocytes like T cells and B cells. However, the precise mechanisms in some cases require further clarification.
The SlideShare 101 is a quick start guide if you want to walk through the main features that the platform offers. This will keep getting updated as new features are launched.
The SlideShare 101 replaces the earlier "SlideShare Quick Tour".
Infectious diseases of livestock are most costly and hazardous problem facing the Agri-food industry
Adversely affect animal production and economics by increasing the cost of production and decreasing the production rate
Breeding Approaches Towards Disease Resistance In LivestocksSharadindu Shil
a detailed description of instances & methodologies used in livestock breeding for developing disease resistant breeds world wide.specially helpful for veterinary post graduate students for their seminars.
This document outlines research work on cloning and silencing genes from the Hyalomma anatolicum anatolicum tick and evaluating the cross-protective efficacy of recombinant proteins. The objectives are to clone and sequence the Subolesin, Calreticulin, and Cathepsin L genes from H. anatolicum anatolicum and Rhipicephalus (Boophilus) microplus ticks. The conservation of these target genes will be evaluated in different isolates of the ticks. RNA interference and immunization trials using recombinant proteins will characterize the target genes of H. anatolicum anatolicum. Previous studies on native and recombinant tick proteins as vaccine targets show variable efficacy, from 15-
The document discusses various aspects of parasite vaccines including:
1. It describes different types of vaccines such as killed whole organisms, attenuated organisms, defined vaccines using purified or recombinant proteins, and DNA vaccines.
2. The key stages in vaccine development are outlined as identifying protective antigens, producing antigens in a commercially viable way, delivering antigens to induce the desired immune response, validating in field trials, and commercial release.
3. Examples of candidate vaccines are provided for many parasite species affecting livestock including Dictyocaulus, Ancylostoma, Haemonchus, Fasciola, Schistosoma, Echinococcus, and Toxoplasma.
Excretion of antibiotic resistance genes by dairy calves fed milk replacers w...Partha Ray
This study examined the effects of feeding dairy calves milk replacer with varying doses of antibiotics on the establishment of antibiotic resistance genes (ARGs) in their feces. Twenty-eight calves were assigned to receive milk replacer containing no antibiotics (control), subtherapeutic doses, or therapeutic doses of tetracycline and neomycin. Fecal samples were collected at weeks 6, 7, and 12 and analyzed for ARGs using quantitative PCR. Surprisingly, antibiotic treatment had little effect on ARG levels, except for slightly higher levels of one tetracycline resistance gene in the highest antibiotic dose group. Several ARGs increased over time in all calves. While antibiotics provided no health benefits, the intensive feeding program used may have overridden any effects
The influence of reduced oxygen availability on gene expression in laboratory...Santhi Devasundaram
Virtually all dormant
models against tuberculosis tested in animals used laboratory strain H37Rv or Erdman strain. But major
outbreaks of tuberculosis (TB) occur with the strains that have widely different genotypes and phenotypes
compared to H37Rv. In this study, we used a custom oligonucleotide microarray to determine the overall
transcriptional response of laboratory strain (H37Rv) and most prevalent clinical strains (S7 and S10) of
M. tuberculosis from South India to hypoxia.
This document summarizes a study on Salmonella in hen eggs in Assiut, Egypt. The researchers found higher rates of Salmonella contamination in balady (native breed) eggs than commercial eggs. Salmonella arizona and Salmonella enteritidis were the most common types isolated. These Salmonella strains showed resistance to most antibiotics tested but sensitivity to Doxveto and Novobiocin. When inoculated into eggs, S. arizona survived longer than S. enteritidis at refrigeration temperatures. Boiling eggs for 10-15 minutes destroyed both pathogens. Proper refrigeration and thorough cooking can reduce Salmonella risks from eggs.
genetic Resistance against gastrointestinal nematodes in sheep: Association w...Ishfaq Maqbool
describes briefly about need of breeding for genetic resistance, candidate genes associated with resistance, genomic regions located on different sheep chromosomes and mechanisms by which the genes act.
Dr. Sid Thakur - Antimicrobial Resistance: Do We Know Everything?John Blue
Antimicrobial Resistance: Do We Know Everything? - Dr. Sid Thakur, Assistant Professor, North Carolina State University, from the 2013 NIAA Merging Values and Technology conference, April 15-17, 2013, Louisville, KY, USA.
More presentations at http://www.trufflemedia.com/agmedia/conference/2013-niaa-merging-values-and-technology
This document discusses ascariasis, a common helminth infection caused by the roundworm Ascaris lumbricoides. It infects over 25% of the world's population, predominantly children. Symptoms can include growth retardation, pneumonia, and intestinal obstruction. The life cycle and immunosuppressive effects of ascariasis are described, including its role in modulating the immune system and suppressing inflammatory responses through molecules like PAS-1. The hygiene hypothesis, which proposes that lack of early childhood exposure to pathogens like helminths may increase risk for allergic diseases, is also discussed.
This document discusses an experiment that measured the sublethal effects of antibiosis resistance in two Brachiaria hybrids on two important spittlebug pest species in Colombia. The experiment found that:
1) Nymphal survival of both spittlebug species was significantly lower and development time significantly longer when reared on the resistant hybrids compared to the susceptible genotype.
2) Median survival times of resulting adult spittlebugs did not differ based on whether nymphs were reared on resistant or susceptible plants, but reproductive biology was negatively impacted for adults from nymphs reared on the resistant hybrids.
3) The high nymphal mortality and sublethal effects on resulting adults caused by
Deployment of broad spectrum resistance against rice blast which includes gene pyramiding, deployment, transgenic approaches, marker assisted back cross breeding, pedigree by using major R genes and QTLs and phytoalexin genes.
1) Transgenic fish models carrying bacteriophage λ and plasmid pUR288 vectors were developed to improve methods for assessing health risks from environmental mutagens and establish new animal models for studying in vivo mutagenesis.
2) The bacteriophage λ transgenic medaka model uses the cII and lacI genes as mutational targets, allowing detection of mutations through packaging of the phage vector and infection of E. coli. Spontaneous mutation frequencies in medaka were comparable to rodent models.
3) Exposure to chemical mutagens like ENU induced concentration-dependent, tissue-specific, and time-dependent increases in cII mutations, demonstrating the utility of the transgenic fish model for studying mut
Katie Flanagan - Malaria vaccines current status and challengesWAidid
Vaccines are considered the most cost-effective means of control, prevention, elimination, eradication of infectious diseases: for this reason, a malaria vaccine would greatly assist in the drive to eradicate malaria from the world. Professor Flanagan presents in this slideset the current status and challenges of developing malaria vaccines.
To learn more, visit www.waidid.org!
This study examined the effects of supplementing broiler chicken feed with a commercial acidifier product containing formaldehyde on performance, Salmonella prevalence, immunity and carcass traits. Three groups of broiler chickens were fed either an unsupplemented control diet or diets with 250g/ton or 500g/ton of the acidifier product. Supplementation improved body weight gain and feed conversion compared to the control. It also reduced signs, mortality, shedding and reisolation of Salmonella typhimurium following an experimental infection. Additionally, supplementation enhanced antibody responses to vaccines and innate immune parameters. Overall, the acidifier product had beneficial effects on broiler growth performance, Salmonella resistance and immunity.
This document describes a study that evaluated the effectiveness of sarafloxacin in controlling experimental colisepticaemia (E. coli infection) in broiler chickens. In the study, 150 broiler chicks were divided into 3 groups: 1) challenged with E. coli but not treated, 2) challenged with E. coli and treated with sarafloxacin, 3) unchallenged and untreated control. The results showed that sarafloxacin treatment significantly improved chicken performance, decreased morbidity/mortality, and reduced E. coli re-isolation compared to the untreated infected group. The study concluded that sarafloxacin administered at 5 mg/kg body weight for 3 days through drinking water is effective for
Dysregulation of macrophage signal transduction by ToxoplasmaIshfaq Maqbool
A brief account of mechanism adopted by Toxoplasma gondii to evade the immune response of the host immune cells particularly macrophages by disruption of macrophage signal transduction
This document discusses the host protective roles of type 2 immunity in response to parasitic infections. It summarizes that type 2 immunity involves both innate and adaptive immune cells that work together to kill parasites and repair tissue damage through mechanisms like alternate macrophage activation. Key cells involved include ILC2s, eosinophils, mast cells, and alternatively activated macrophages that secrete molecules like IL-4, IL-5, IL-13, and arginase to expel parasites and promote wound healing.
Immunity against Helminths:role of InterleukinsIshfaq Maqbool
The document summarizes key aspects of the immune response against helminth parasites. It notes that helminths typically induce a type 2 immune response characterized by cytokines like IL-4, IL-5, and IL-13. This non-inflammatory response involves alternatively activated macrophages, eosinophils, and other effector cells that work to expel and kill parasites while repairing tissue damage. The response differs from bacterial and viral immunity, with Th1 responses only occurring during early larval migration stages.
Autophagy plays multifaceted roles in both the innate and adaptive immune system. In innate immunity, autophagy aids in the recognition of pathogens by pattern recognition receptors like TLRs, NLRs, and RLRs. It also facilitates the handling and destruction of intracellular bacteria by phagocytosis and lysosomal fusion. Autophagy regulates the production of cytokines as well. In adaptive immunity, autophagy contributes to antigen presentation on MHC class I and II molecules and is important for the homeostasis and functions of lymphocytes like T cells and B cells. However, the precise mechanisms in some cases require further clarification.
The SlideShare 101 is a quick start guide if you want to walk through the main features that the platform offers. This will keep getting updated as new features are launched.
The SlideShare 101 replaces the earlier "SlideShare Quick Tour".
Infectious diseases of livestock are most costly and hazardous problem facing the Agri-food industry
Adversely affect animal production and economics by increasing the cost of production and decreasing the production rate
Breeding Approaches Towards Disease Resistance In LivestocksSharadindu Shil
a detailed description of instances & methodologies used in livestock breeding for developing disease resistant breeds world wide.specially helpful for veterinary post graduate students for their seminars.
This document outlines research work on cloning and silencing genes from the Hyalomma anatolicum anatolicum tick and evaluating the cross-protective efficacy of recombinant proteins. The objectives are to clone and sequence the Subolesin, Calreticulin, and Cathepsin L genes from H. anatolicum anatolicum and Rhipicephalus (Boophilus) microplus ticks. The conservation of these target genes will be evaluated in different isolates of the ticks. RNA interference and immunization trials using recombinant proteins will characterize the target genes of H. anatolicum anatolicum. Previous studies on native and recombinant tick proteins as vaccine targets show variable efficacy, from 15-
The document discusses various aspects of parasite vaccines including:
1. It describes different types of vaccines such as killed whole organisms, attenuated organisms, defined vaccines using purified or recombinant proteins, and DNA vaccines.
2. The key stages in vaccine development are outlined as identifying protective antigens, producing antigens in a commercially viable way, delivering antigens to induce the desired immune response, validating in field trials, and commercial release.
3. Examples of candidate vaccines are provided for many parasite species affecting livestock including Dictyocaulus, Ancylostoma, Haemonchus, Fasciola, Schistosoma, Echinococcus, and Toxoplasma.
Excretion of antibiotic resistance genes by dairy calves fed milk replacers w...Partha Ray
This study examined the effects of feeding dairy calves milk replacer with varying doses of antibiotics on the establishment of antibiotic resistance genes (ARGs) in their feces. Twenty-eight calves were assigned to receive milk replacer containing no antibiotics (control), subtherapeutic doses, or therapeutic doses of tetracycline and neomycin. Fecal samples were collected at weeks 6, 7, and 12 and analyzed for ARGs using quantitative PCR. Surprisingly, antibiotic treatment had little effect on ARG levels, except for slightly higher levels of one tetracycline resistance gene in the highest antibiotic dose group. Several ARGs increased over time in all calves. While antibiotics provided no health benefits, the intensive feeding program used may have overridden any effects
The influence of reduced oxygen availability on gene expression in laboratory...Santhi Devasundaram
Virtually all dormant
models against tuberculosis tested in animals used laboratory strain H37Rv or Erdman strain. But major
outbreaks of tuberculosis (TB) occur with the strains that have widely different genotypes and phenotypes
compared to H37Rv. In this study, we used a custom oligonucleotide microarray to determine the overall
transcriptional response of laboratory strain (H37Rv) and most prevalent clinical strains (S7 and S10) of
M. tuberculosis from South India to hypoxia.
This document summarizes a study on Salmonella in hen eggs in Assiut, Egypt. The researchers found higher rates of Salmonella contamination in balady (native breed) eggs than commercial eggs. Salmonella arizona and Salmonella enteritidis were the most common types isolated. These Salmonella strains showed resistance to most antibiotics tested but sensitivity to Doxveto and Novobiocin. When inoculated into eggs, S. arizona survived longer than S. enteritidis at refrigeration temperatures. Boiling eggs for 10-15 minutes destroyed both pathogens. Proper refrigeration and thorough cooking can reduce Salmonella risks from eggs.
genetic Resistance against gastrointestinal nematodes in sheep: Association w...Ishfaq Maqbool
describes briefly about need of breeding for genetic resistance, candidate genes associated with resistance, genomic regions located on different sheep chromosomes and mechanisms by which the genes act.
Dr. Sid Thakur - Antimicrobial Resistance: Do We Know Everything?John Blue
Antimicrobial Resistance: Do We Know Everything? - Dr. Sid Thakur, Assistant Professor, North Carolina State University, from the 2013 NIAA Merging Values and Technology conference, April 15-17, 2013, Louisville, KY, USA.
More presentations at http://www.trufflemedia.com/agmedia/conference/2013-niaa-merging-values-and-technology
This document discusses ascariasis, a common helminth infection caused by the roundworm Ascaris lumbricoides. It infects over 25% of the world's population, predominantly children. Symptoms can include growth retardation, pneumonia, and intestinal obstruction. The life cycle and immunosuppressive effects of ascariasis are described, including its role in modulating the immune system and suppressing inflammatory responses through molecules like PAS-1. The hygiene hypothesis, which proposes that lack of early childhood exposure to pathogens like helminths may increase risk for allergic diseases, is also discussed.
This document discusses an experiment that measured the sublethal effects of antibiosis resistance in two Brachiaria hybrids on two important spittlebug pest species in Colombia. The experiment found that:
1) Nymphal survival of both spittlebug species was significantly lower and development time significantly longer when reared on the resistant hybrids compared to the susceptible genotype.
2) Median survival times of resulting adult spittlebugs did not differ based on whether nymphs were reared on resistant or susceptible plants, but reproductive biology was negatively impacted for adults from nymphs reared on the resistant hybrids.
3) The high nymphal mortality and sublethal effects on resulting adults caused by
Deployment of broad spectrum resistance against rice blast which includes gene pyramiding, deployment, transgenic approaches, marker assisted back cross breeding, pedigree by using major R genes and QTLs and phytoalexin genes.
1) Transgenic fish models carrying bacteriophage λ and plasmid pUR288 vectors were developed to improve methods for assessing health risks from environmental mutagens and establish new animal models for studying in vivo mutagenesis.
2) The bacteriophage λ transgenic medaka model uses the cII and lacI genes as mutational targets, allowing detection of mutations through packaging of the phage vector and infection of E. coli. Spontaneous mutation frequencies in medaka were comparable to rodent models.
3) Exposure to chemical mutagens like ENU induced concentration-dependent, tissue-specific, and time-dependent increases in cII mutations, demonstrating the utility of the transgenic fish model for studying mut
Katie Flanagan - Malaria vaccines current status and challengesWAidid
Vaccines are considered the most cost-effective means of control, prevention, elimination, eradication of infectious diseases: for this reason, a malaria vaccine would greatly assist in the drive to eradicate malaria from the world. Professor Flanagan presents in this slideset the current status and challenges of developing malaria vaccines.
To learn more, visit www.waidid.org!
This study examined the effects of supplementing broiler chicken feed with a commercial acidifier product containing formaldehyde on performance, Salmonella prevalence, immunity and carcass traits. Three groups of broiler chickens were fed either an unsupplemented control diet or diets with 250g/ton or 500g/ton of the acidifier product. Supplementation improved body weight gain and feed conversion compared to the control. It also reduced signs, mortality, shedding and reisolation of Salmonella typhimurium following an experimental infection. Additionally, supplementation enhanced antibody responses to vaccines and innate immune parameters. Overall, the acidifier product had beneficial effects on broiler growth performance, Salmonella resistance and immunity.
This document describes a study that evaluated the effectiveness of sarafloxacin in controlling experimental colisepticaemia (E. coli infection) in broiler chickens. In the study, 150 broiler chicks were divided into 3 groups: 1) challenged with E. coli but not treated, 2) challenged with E. coli and treated with sarafloxacin, 3) unchallenged and untreated control. The results showed that sarafloxacin treatment significantly improved chicken performance, decreased morbidity/mortality, and reduced E. coli re-isolation compared to the untreated infected group. The study concluded that sarafloxacin administered at 5 mg/kg body weight for 3 days through drinking water is effective for
1) The study examined the effects of diethyl hexyl phthalate (DEHP) exposure on insulin signaling molecules and glucose metabolism in the triceps muscle of rats.
2) Rats treated with DEHP showed increased oxidative stress, reduced insulin receptor and glucose transporter 4 (GLUT4) levels, and decreased glucose uptake and oxidation in the triceps muscle.
3) Co-treatment with antioxidants vitamins C and E prevented the adverse effects of DEHP by reducing oxidative stress, and protecting insulin signaling molecule levels and glucose metabolism in the triceps muscle.
The document discusses the RTS,S/AS01 malaria vaccine candidate. It provides details on the development of RTS,S from initial designs incorporating the circumsporozoite protein to clinical trials demonstrating safety and efficacy. Phase 3 trials in African children showed RTS,S/AS01 reduced clinical malaria by approximately 50% and severe malaria by approximately 50% over 12 months. While the vaccine provided benefit, it also caused some adverse side effects and deaths. Ongoing research continues to improve vaccine design and development for a highly effective malaria vaccine.
Predict of coronary artery lesions in Kawasaki disease (川崎症-郭和昌醫師)Ho-Chang Kuo (郭和昌 醫師)
The document discusses predicting coronary artery aneurysm (CAL) formation in Kawasaki disease (KD). It describes the clinical presentation and diagnostic criteria of KD. Treatment involves high-dose intravenous immunoglobulin (IVIG) and aspirin. Some patients are resistant to initial IVIG treatment. Genetic studies have found associations between certain single nucleotide polymorphisms and susceptibility to KD or CAL formation.
The Best Dietetic Principles To Life Extensionswissestetix
Das ist ein Vortrag, den Dr. Clarence P. Davis im Jahre 2007 im Rahmen eines Anti-Aging Kongresses in Paris gehalten hat.
Er beinhaltet theoretisches Basis- und Hintergrundswissen zu den verschiedenen Diaettypen, sowie einige praktische Beispiele aus dem aerztlichen Alltag.
Der Vortrag ist auf Englisch und einem hohen Niveau. Er richtet sich ausschliesslich an professionelle Leser mit fundierten Vorkenntnissen.
Similar to Vaccination against Haemonchus contortus (20)
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...AbdullaAlAsif1
The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
ESPP presentation to EU Waste Water Network, 4th June 2024 “EU policies driving nutrient removal and recycling
and the revised UWWTD (Urban Waste Water Treatment Directive)”
ESR spectroscopy in liquid food and beverages.pptxPRIYANKA PATEL
With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptxMAGOTI ERNEST
Although Artemia has been known to man for centuries, its use as a food for the culture of larval organisms apparently began only in the 1930s, when several investigators found that it made an excellent food for newly hatched fish larvae (Litvinenko et al., 2023). As aquaculture developed in the 1960s and ‘70s, the use of Artemia also became more widespread, due both to its convenience and to its nutritional value for larval organisms (Arenas-Pardo et al., 2024). The fact that Artemia dormant cysts can be stored for long periods in cans, and then used as an off-the-shelf food requiring only 24 h of incubation makes them the most convenient, least labor-intensive, live food available for aquaculture (Sorgeloos & Roubach, 2021). The nutritional value of Artemia, especially for marine organisms, is not constant, but varies both geographically and temporally. During the last decade, however, both the causes of Artemia nutritional variability and methods to improve poorquality Artemia have been identified (Loufi et al., 2024).
Brine shrimp (Artemia spp.) are used in marine aquaculture worldwide. Annually, more than 2,000 metric tons of dry cysts are used for cultivation of fish, crustacean, and shellfish larva. Brine shrimp are important to aquaculture because newly hatched brine shrimp nauplii (larvae) provide a food source for many fish fry (Mozanzadeh et al., 2021). Culture and harvesting of brine shrimp eggs represents another aspect of the aquaculture industry. Nauplii and metanauplii of Artemia, commonly known as brine shrimp, play a crucial role in aquaculture due to their nutritional value and suitability as live feed for many aquatic species, particularly in larval stages (Sorgeloos & Roubach, 2021).
The binding of cosmological structures by massless topological defectsSérgio Sacani
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
hematic appreciation test is a psychological assessment tool used to measure an individual's appreciation and understanding of specific themes or topics. This test helps to evaluate an individual's ability to connect different ideas and concepts within a given theme, as well as their overall comprehension and interpretation skills. The results of the test can provide valuable insights into an individual's cognitive abilities, creativity, and critical thinking skills
When I was asked to give a companion lecture in support of ‘The Philosophy of Science’ (https://shorturl.at/4pUXz) I decided not to walk through the detail of the many methodologies in order of use. Instead, I chose to employ a long standing, and ongoing, scientific development as an exemplar. And so, I chose the ever evolving story of Thermodynamics as a scientific investigation at its best.
Conducted over a period of >200 years, Thermodynamics R&D, and application, benefitted from the highest levels of professionalism, collaboration, and technical thoroughness. New layers of application, methodology, and practice were made possible by the progressive advance of technology. In turn, this has seen measurement and modelling accuracy continually improved at a micro and macro level.
Perhaps most importantly, Thermodynamics rapidly became a primary tool in the advance of applied science/engineering/technology, spanning micro-tech, to aerospace and cosmology. I can think of no better a story to illustrate the breadth of scientific methodologies and applications at their best.
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...Sérgio Sacani
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
2. Introduction
Haemonchus contortus
Ubiquitous and highly pathogenic.
Mostly in tropical and sub-tropical countries
(Kalita et al., 1978)
Temperate regions with focal areas of similar climatic conditions
(Waller et al., 2004; Waller et al., 2006)
In India
- 3 species of Haemonchus
- H. contortus, H. longistipes and H. similis
(Sood, 1981)
4. A female lays around 5000-10000 eggs/day
0.05 – 0.07 ml/parasite/day leading to anaemia.
(Malviya et al.,1979; Soulsby, 1982)
Punctiform haemorrhages in abomasal mucosa
Hypoproteinemia, bottle jaw
↓ Digestion and absorption of proteins, calcium and phosphorus (Sood, 1981)
Decreased feed conversion, malnutrition, loss of appetite, low fertility indices and in certain
cases death of young animals
(Quiroz et al., 1984)
5. Economic losses are incurred through morbidity, mortality and increased investment
due to cost of preventative as well as curative treatments
(Miller et al., 2006)
GI parasites in sheep alone cause annual losses ranging from US $ 42 million to US $
222 million
(Waller, 2006)
Losses due to GI parasites in sheep was estimated to be US $500 million in Australia
and $946 million in New Zealand
(Emery, 1991;Vlassoff and McKenna, 1994)
Estimated treatment cost alone for H. contortus per year in India was US $103 million
(McLeod, 2004)
Chronic haemonchosis is very common and of considerable economic importance
(Newton and Munn, 1999)
Economic Importance
6. Anthelminthic resistance
(Kaplan et al., 2005; Burke and Miller, 2006)
Demand for “clean and green” animal products free of residual chemicals and growth
promoters
(Waller, 2003)
Increasing toxicity and persistence of chemical residues in the environment
(Wolstenholme et al., 2004)
Immunization with irradiated L3 stage larvae
(Mulligan et al., 1961)
- Failure to protect young lambs
(Smith and Christie, 1979)
Need for Immunoprophylaxis
7. How it works?
Adequate control can be achieved with vaccine efficacies of about 80%
(Barnes et al. 1995)
8. Natural or Surface or E/S antigens
(Emery et al., 1999; Alunda et al., 2003)
Hidden or Concealed antigens
(Smith, 1999; Knox et al., 1999)
Types of Antigens
9. Schallig et al 1997 identified adult 15 and 24 kDa as immunogenic polypeptides in
ES products of H.contortus
Gomez-Munoz et al., 1995 identified and purified 26 kDa somatic antigen but
Cornelissen, 1996 concluded that 26-kDa Somatic and the 24-kDa E/S antigens of
Haemonchus contortus one and the same differing in glycosylation
Karanu et al.,1993 identified Cysteine proteinases in excretory-secretory (ES)
products with estimated molecular weights (Mr) of 32, 35, 38, and 40 kDa
Natural antigens
10. Gut antigens of fourth larval stage (L4) and adult parasites
Contortin: (Munn, 1977 )
Helical polymeric structure attached to the luminal surface of the intestinal cells
Aminopeptidase H11 glycoprotein: (Munn et al.,1992 )
It runs on (SDS-PAGE) as a doublet with a mean value of 110 kDa
It has microsomal (membrane) aminopeptidase M and microsomal aminopeptidase A
activities attributable to distinct isoforms
(Smith et al 1997, Graham et
al 1993)
Haemonchus galactose-containing glycoprotein complex: (Smith et al., 1994 )
It contains aspartyl, metallo- and cysteine proteinases. Major component is a family of four
zinc metallo-endopeptidases, designated MEPs 1–4, MEP3 being most dominant
(Smith et al., 1999)
Hidden antigens…
11. p52 and p46: Mixture of two gut surface proteins of 52 and 46 kDa obtained by affinity
chromatography
(Sharp et al., 1992)
Cysteine proteinases and Glutamate dehydrogenase :
The expression of the GDH and cysteine protease encoding genes coincides with the onset
of blood-feeding
Cysteine proteinase:
Cathepsin-B family expressed in intestine of adult worms
(Rehman and Jasmer, 1999;Skuce et al., 1999)
Cysteine proteases in H. contortus are capable of digesting Hb, fibrinogen, collagen and
IgG (Knox et al., 1993)
GDH:
Predominant portion of S3 TSBP of 60 kDa (Knox et al. 1995)
Expressed in the cytoplasm of the intestinal cells (Skuce et al., 1999)
Hidden antigens…
12. Fractionate and vaccinate approach
(Emery and Wagland, 1991)
Gut antigens
H11 (Munn et al., 1993)
H-gal-GP (Smith et al., 1994, 1999)
Cysteine protease (Knox et al., 1995)
Use of immune serum
15 and 24 kDa ES Ag (Schallig et al., 1994)
Use of ASC probes
HcsL3 (Meeusen et al., 1995)
Identification of Immunodominant antigens
13. Antigen Animal
specifications
Protection Immunoglobulin Reference
r15/24 Lambs
Adults
55% FECR
49% FECR
Low IgA,IgG,IgE level Vervelde et al.,2002
rHcp26/23 All age groups No significant protection Strong IgG and
Lymphoproliferative
response
Leticia et al .,2010
rH11 6 m old lambs No significant protection - Sexton and Zawadski .,2005
H-gal-GP Blackface × Leicest
er male lambs, aged
9 months
No significant protection High levels of
immunoglobulins
Smith et al.,2003
Cachet et al.,2010
Cysteine
Proteinase
Hc58
Sheep
Goat (7-8 m)
29-38%
Slight protection
Strong correlation
Increase in IgA,IgG and
Mucosal IgA level
Redmond and Knox.,2004
Muleke et al.,2007
rHc23 Lambs (6-7 m) >80% FEC
85% WB reduction
Increased level of
IgA,IgG,IgE
Fawzi et al.,2015
Vaccination with recombinant Ag’s
14. Recombinant
Antigen
Reason/s for Failure Reference
15 and 24 kDa Redundancy Newton and Meeusen.,2003
p26/23 Lack of glycosylation Leticia et al .,2010
H-gal-GP Inappropriate glycosylation and/or
conformational folding
Smith et al., 2003a
Smith et al., 2003b
H-gal-GP Failure to identify the protective component
of the complex, or the requirement for the
antigen(s) to be presented in a complex form
Newton and Meeusen.,2003
H11 Inappropriate glycosylation and/or
conformational folding
Haslam et al., 1996
Munn et al.,1997
Newton and Meeusen.,2003
H11 Lack of additional components that are
present in native H11-enriched extract
Roberts et al., 2013
Cysteine
proteinase
Inappropriate folding Redmond and Knox.,2004
Failure of vaccination with rAg
15. Antigen/dose Adjuvant Animal
specifications
Efficacy Immunoglobulin Reference
15 and 24 kDa
50-100µg
DDA Lambs 72.9%FEC reduction
82.2% WB reduction
Strong correlation Schallig & van
Leeuwen, 1997;
Schallig et al., 1997
15 and 24 kDa
50-100µg
DDA Sheep 82% WB reduction in
9 m old
77% in 6m old
0% in 3m old
Strong correlation Kooyman et al.,2000
p26/23 DDA Lambs >60% FEC
>61.6% WB
reduction
Strong correlation Domınguez-Torano et
al., 2000
Cysteine protease Aluminum
hydroxide
Lambs 77%FEC reduction
and 47% WB
reduction
Significantly higher
local and systemic
ES specific IgA and
IgG responses.
Boisvenue et al., 1992
Bakker et al., 2004
De Vries et al. (2009)
Hc-sL3
20 µg
Aluminum
hydroxide
Five month old
lambs
61% FEC reduction
69% WB reduction
No difference Jacobs et al., 1999
Hc23
100 µg
Al(OH)3
Bacterial
Lambs 70.67%,85.64% FEC
67.1% , 86% WB
reduction
respectively
Strong correlation Fawzi et al., 2014
Vaccination with native natural Ag
16. Antigen Animal
specifications
Efficacy Immunoglobulin
levels
Reference
Contortin Lambs 75% reduction in worm burden Strongly correlated Munn et al., 1987
p52 and p46 Lambs 78% FEC reduction
33%WB reduction
- Smith et al., 2000
p52 and p46 14-month-
old goats
34% FEC reduction and 53%
WB reduction
- Jasmer et al., 1993
Cysteine proteinase
S3 TSBP
S3 TSBP
S3 TSBP
Purified cysteine protease
S3 TSBP
Purified cysteine protease
Sheep
Goats
Lambs
Lambs
Lambs
95% FECR,50% WBR
77% FECRand 47%WBR
89% FECRand 68%WBR
Comparable results
Significant protection
Significant protection
Predominant IgG2 response
High IgG levels
High levels of antibody
Knox et al., 1995
Knox et al.,1999
Ruiz et al., 2004
Knox et al., 2005
Redmond et al., 2006
Molina et al., 2015
Vaccination with native hidden Ag
17. Antigen/dose/adju
vant
Animal
specifications
Efficacy Immunoglobulin
levels
Reference
H11 50 µg
FCA/FIA
Young Dorset lambs 78% FECR
83% WB reduction
Significant
correlation
Tavernor et al. 1992
H11 fraction
0.35µg FCA/FIA
Young merino lambs 70.7% FECR
70% WB reduction
Significant
correlation
Munn et al., 1993
H11 140µg
H11 200µg
4 month old lambs 94.6% FEC reduction
86.5% and 93.5% male and
female WB reduction
respectively
Significant
correlation
Smith et al., 1994
H11 from
resistant
strains150µg
FCA/FIA
Lambs 99% FECR
90% WB reduction
Significant
correlation
Newton et al., 1994
H11 (4
isolates)150µg
FCA/FIA
Lambs 82–96% FECR 55.9–93.8%WB
reduction
Significant
correlation
Newton et al., 1994
H11 50 µg
FCA/FIA .
Pregnant ewes 91% FECR
86% WB reduction
Significantly higher,
Colostral transfer
Andrews et al., 1995
H11 40 µg Vax
saponin
Lambs 99.9% FECR
93.6% WB reduction
Significant
correlation
Roberts et al.., 2013
Vaccination with native H11
18. Antigen/dose/adjuv
ant
Animal
specifications
Efficacy Immunoglobulin
levels
Reference
H-gal-GP 200 µg in
FCA
Suffolk x Dorset 3-5
m old lams
89% FECR
69.5% WB
reduction
Significant
correlation
Smith & Smith .,1996
H-gal-GP 100µg
FCA/FIA
5-6 m old lambs 56.5–69.7 % FECR
40–53.5%% WB
reduction
Significant
correlation
Smith et al. ,1999
H-gal-GP 100µg
Quil A
Lambs 83.4% WB
reduction
Significant
correlation
Newlands et al.., 1999
H-gal-GP 100 µg
Quil A
Lambs 93% FECR
60–64%WB
reduction
Significant
correlation
Smith et al. ,2000
Smith et al. ,2003
Smith et al. ,2007
H-gal-GP 100 µg
Quil A
Lambs 88.5% FECR
72.3% WB
reduction
Significant
correlation
Cachat et al. ,2010
Vaccination with native H-gal-GP
19. Location Animal
specification
Antigen ,dose and
adjuvant
Efficacy Reference
Lousiana,USA Suffolk ewes>2y 100 µg H11+100 µg H-
gal-GP in 5 µg Quil A
>65%FECR Kabagambe et al., 2000
South Africa 12-18 m old Dorper
sheep
100 µg H11+100 µg
H-gal-GP in 5 µg Quil
A
>82% FECR Smith et al., 2001
Australia Grazing merino
lambs
100 µg H11+100 µg H-
gal-GP in 5 µg Quil A
>85% FECR
High levels of
IgG1 and
IgG2
LeJambre et al., 2008
Brazil Periparturient
ewes
5 or 50 µg H11 and 1mg
of saponin as adjuvant
78% FECR Bassetto et al., 2014
Field trials in sheep
20. 1st
commercially available sub-unit vaccine against H. contortus developed by Dr.
David Smith of Moredun Research Institute, Edinburgh , UK.
Manufactured by Dept. of Agriculture and Food, Albany, Western Australia
5µg native gut integral protein + 1mg saponin adjuvant /dose (1ml Subcutaneously)
5 doses for lambs during the summer Haemonchus risk period
Reduces the periparturient rise – epidemiological benefit to flock