Dr. Paul Turek’s Society for the Study of Male Reproduction (SSMR) presentation at the American Urology Association (AUA) annual conference in Orlando, FL on Tuesday, May 20, 2014.
Fertility Restoration after Cancer: Current and Future Therapies By Paul J. ...The Turek Clinics
Urologist and male fertility doctor for vasectomy and vasectomy reversal, sperm retrieval, testicular mapping, varicocele repair and ejaculatory duct repair, Dr. Paul Turek, speaks about Fertility Restoration after Cancer: Current and Future Therapies. Dr. Turek is director of The Turek Clinic. Located in San Francisco, California, The Turek Clinic provides world-class patient care with an essential holistic approach. (WARNING: Images in slides not appropriate for all audiences due to subject matter.)
Clinical management of men with nonobstructive azoospermia - Azoospermia Diff...Sandro Esteves
Reproductive Andrology Workshop III
17-21 January 2016 - Kuwait City - KUWAIT
Organized by: Al Jahra Reproductive Medicine Unit - Ministry of Health
Lecture 1: Azoospermia Differential Diagnosis
Fertility Restoration after Cancer: Current and Future Therapies By Paul J. ...The Turek Clinics
Urologist and male fertility doctor for vasectomy and vasectomy reversal, sperm retrieval, testicular mapping, varicocele repair and ejaculatory duct repair, Dr. Paul Turek, speaks about Fertility Restoration after Cancer: Current and Future Therapies. Dr. Turek is director of The Turek Clinic. Located in San Francisco, California, The Turek Clinic provides world-class patient care with an essential holistic approach. (WARNING: Images in slides not appropriate for all audiences due to subject matter.)
Clinical management of men with nonobstructive azoospermia - Azoospermia Diff...Sandro Esteves
Reproductive Andrology Workshop III
17-21 January 2016 - Kuwait City - KUWAIT
Organized by: Al Jahra Reproductive Medicine Unit - Ministry of Health
Lecture 1: Azoospermia Differential Diagnosis
Public lecture - Stem Cell and Male InfertilitySandro Esteves
Reproductive Andrology Workshop III
17-21 January 2016 - Kuwait City - KUWAIT
Organized by: Al Jahra Reproductive Medicine Unit - Ministry of Health
Public Lecture - Stem Cell and Male Infertility
Clinical management of men with nonobstructive azoospermia - Role of IVF Labo...Sandro Esteves
Reproductive Andrology Workshop III
17-21 January 2016 - Kuwait City - KUWAIT
Organized by: Al Jahra Reproductive Medicine Unit - Ministry of Health
Lecture 5: Role of IVF Laboratory in Nonobstructive Azoospermia
American Urological Association (AUA) Lecture given at the American Society of Andrology (ASA) 40th annual conference, April 18 – 21, 2015 in Salt Lake City, Utah.
Azoospermia is an challenging subject either on the diagnostic side or on the therapeutic issues. Types of testicular biopsy must be employed in selected patients as regard their background diagnosis e.g. obstructive, Klinefelter's,... etc.
There I go again, a Western guy giving a lecture to an Eastern crowd. What team do I play on, you ask? In fact, I am honored to give a keynote at the First Integrative Fertility Symposium in Vancouver. Ok, call me a “swingman,” but the Easterners have a lot up their medical sleeves too. Ask Western medicine how to help a guy relax, and they’ll say, “don’t work so hard and take this pill.” Ask an Easterner, and they might suggest acupuncture, mindfulness and meditation. Which approach is better: a patch or a fix? You decide. Read more on my blog at > http://bit.ly/1EMuRFF
Novel concepts in male factor infertility: clinical and laboratory perspectivesSandro Esteves
Presentation Objectives:
1. Update on the WHO reference values for semen parameters, and understand the role of sperm DNA fragmentation testing to decision-making strategies;
2. Learn how to counsel azoospermic men seeking fertility, and the role of gonadotropin therapy in this infertility condition;
3. Understand the benefits of microsurgery to both sperm retrieval and varicocele treatment;
4. Appraise the role of medical and surgical interventions to infertile men undergoing ART.
Clinical management of men with nonobstructive azoospermia - Steps Before Spe...Sandro Esteves
Reproductive Andrology Workshop III
17-21 January 2016 - Kuwait City - KUWAIT
Organized by: Al Jahra Reproductive Medicine Unit - Ministry of Health
Lecture 3: Steps Before Sperm Retrieval in Nonobstructive Azoospermia
Iran march 2011
ABRASCT:
SPERM RETRIEVAL TECHNIQUES FOR THE AZOOSPERMIC MALE
Sandro C. Esteves, MD, PhD
Spermatozoa can be retrieved from either the epididymis or the testis, depending on the type of azoospermia, using different surgical methods such as PESA, MESA, TESA, TESE and micro-TESE.
In obstructive azoospermia (OA), sperm production is normal and gametes can be easily retrieved from the epididymis or the testicle in most cases, irrespective of the technique. PESA or TESA are simple and efficient methods for retrieving epididymal or testicular spermatozoa in men with OA. According to our data on OA, the etiology of the obstruction and the use of fresh or frozen-thawed epididymal/testicular sperm do not seem to affect ICSI outcomes in terms of fertilization, pregnancy, or miscarriage rates.
In cases of nonobstructive azoospermia (NOA), the efficiency of TESA for retrieving spermatozoa is lower than TESE, except in the favorable cases of men with previous successful TESA or testicular histopathology showing hypospermatogenesis. The use of microsurgery during TESE may improve the efficacy of sperm extraction with significantly less tissue removed, which ultimately facilitates sperm processing. Testicular histology results, if available, may be useful to predict the chances to retrieve sperm in men with NOA. Our data demonstrate that micro-TESE performs better than conventional TESE or TESA in cases of maturation arrest and Sertoli cell-only histological patterns, where tubules containing active focus of spermatogenesis can be positively identified using microsurgery. Testicular spermatozoa can be obtained even in the worst case scenario except in the cases of Y chromosome infertility with complete AZFa and/or AZFbmicrodeletions.
In both OA and NOA, sperm retrieval technique itself seems to have no impact on ICSI success rates. The main goal of PESA/TESA/TESE sperm processing is the recovery of a clean sample containing motile sperm. Such specimens are more fragile, and often compromised in motility, as compared to the ones obtained from ejaculates. Laboratory techniques should be carried out with great caution not to jeopardize the sperm fertilizing potential. Surgically-retrieved spermatozoa can be intentionally cryopreserved for future use. Spare left-over specimens that would be discharged after ICSI can also be cryostored. Different strategies can be developed according to each group’s results. If freezing of surgically-retrieved specimens provides results similar to those with the use of fresh sperm, then the use of freezing specimens would be preferable. If not, fresh specimens are preferable.
The reproductive potential of infertile men undergoing ART is related to the type of azoospermia. According to our data, the chances of retrieving spermatozoa (odds ratio [OR] = 43.0; 95% confidence interval [CI]: 10.3-179.5) and of achieving a live birth by ICSI (OR=1.86; 95% CI:l 1.03-2.89) were significantly increased in couples whose male partner had obstructive rather than non-obstructive azoospermia. Children conceived using sperm retrieved from men with OA and NOA should be followed-up because it is still unclear if there is an increased risk of birth defects when ICSI is carried out with non-ejaculated sperm.
References
Esteves SC, Glina S. Recovery of spermatogenesis after microsurgical subinguinal varicocele repair in azoospermic men based on testicular histology. IntBraz J Urol. 2005; 31:541-8.
Verza S Jr, Esteves SC. Sperm defect severity rather than sperm source is associated with lower fertilization rates after intracytoplasmic sperm injection. IntBraz J Urol. 2008,34:49-56.
Esteves SC, Verza S, Prudencio C, Seol B. Sperm retrieval rates (SRR) in nonobstructive azoospermia (NOA) are related to testicular histopathology results but not to the etiology of azoospermia. FertilSteril. 2010; 94(Suppl.):S132.
Esteves SC, Verza S, Prudencio C, Seol B. Success of percutaneous sperm retrieval and i
Public lecture - Stem Cell and Male InfertilitySandro Esteves
Reproductive Andrology Workshop III
17-21 January 2016 - Kuwait City - KUWAIT
Organized by: Al Jahra Reproductive Medicine Unit - Ministry of Health
Public Lecture - Stem Cell and Male Infertility
Clinical management of men with nonobstructive azoospermia - Role of IVF Labo...Sandro Esteves
Reproductive Andrology Workshop III
17-21 January 2016 - Kuwait City - KUWAIT
Organized by: Al Jahra Reproductive Medicine Unit - Ministry of Health
Lecture 5: Role of IVF Laboratory in Nonobstructive Azoospermia
American Urological Association (AUA) Lecture given at the American Society of Andrology (ASA) 40th annual conference, April 18 – 21, 2015 in Salt Lake City, Utah.
Azoospermia is an challenging subject either on the diagnostic side or on the therapeutic issues. Types of testicular biopsy must be employed in selected patients as regard their background diagnosis e.g. obstructive, Klinefelter's,... etc.
There I go again, a Western guy giving a lecture to an Eastern crowd. What team do I play on, you ask? In fact, I am honored to give a keynote at the First Integrative Fertility Symposium in Vancouver. Ok, call me a “swingman,” but the Easterners have a lot up their medical sleeves too. Ask Western medicine how to help a guy relax, and they’ll say, “don’t work so hard and take this pill.” Ask an Easterner, and they might suggest acupuncture, mindfulness and meditation. Which approach is better: a patch or a fix? You decide. Read more on my blog at > http://bit.ly/1EMuRFF
Novel concepts in male factor infertility: clinical and laboratory perspectivesSandro Esteves
Presentation Objectives:
1. Update on the WHO reference values for semen parameters, and understand the role of sperm DNA fragmentation testing to decision-making strategies;
2. Learn how to counsel azoospermic men seeking fertility, and the role of gonadotropin therapy in this infertility condition;
3. Understand the benefits of microsurgery to both sperm retrieval and varicocele treatment;
4. Appraise the role of medical and surgical interventions to infertile men undergoing ART.
Clinical management of men with nonobstructive azoospermia - Steps Before Spe...Sandro Esteves
Reproductive Andrology Workshop III
17-21 January 2016 - Kuwait City - KUWAIT
Organized by: Al Jahra Reproductive Medicine Unit - Ministry of Health
Lecture 3: Steps Before Sperm Retrieval in Nonobstructive Azoospermia
Iran march 2011
ABRASCT:
SPERM RETRIEVAL TECHNIQUES FOR THE AZOOSPERMIC MALE
Sandro C. Esteves, MD, PhD
Spermatozoa can be retrieved from either the epididymis or the testis, depending on the type of azoospermia, using different surgical methods such as PESA, MESA, TESA, TESE and micro-TESE.
In obstructive azoospermia (OA), sperm production is normal and gametes can be easily retrieved from the epididymis or the testicle in most cases, irrespective of the technique. PESA or TESA are simple and efficient methods for retrieving epididymal or testicular spermatozoa in men with OA. According to our data on OA, the etiology of the obstruction and the use of fresh or frozen-thawed epididymal/testicular sperm do not seem to affect ICSI outcomes in terms of fertilization, pregnancy, or miscarriage rates.
In cases of nonobstructive azoospermia (NOA), the efficiency of TESA for retrieving spermatozoa is lower than TESE, except in the favorable cases of men with previous successful TESA or testicular histopathology showing hypospermatogenesis. The use of microsurgery during TESE may improve the efficacy of sperm extraction with significantly less tissue removed, which ultimately facilitates sperm processing. Testicular histology results, if available, may be useful to predict the chances to retrieve sperm in men with NOA. Our data demonstrate that micro-TESE performs better than conventional TESE or TESA in cases of maturation arrest and Sertoli cell-only histological patterns, where tubules containing active focus of spermatogenesis can be positively identified using microsurgery. Testicular spermatozoa can be obtained even in the worst case scenario except in the cases of Y chromosome infertility with complete AZFa and/or AZFbmicrodeletions.
In both OA and NOA, sperm retrieval technique itself seems to have no impact on ICSI success rates. The main goal of PESA/TESA/TESE sperm processing is the recovery of a clean sample containing motile sperm. Such specimens are more fragile, and often compromised in motility, as compared to the ones obtained from ejaculates. Laboratory techniques should be carried out with great caution not to jeopardize the sperm fertilizing potential. Surgically-retrieved spermatozoa can be intentionally cryopreserved for future use. Spare left-over specimens that would be discharged after ICSI can also be cryostored. Different strategies can be developed according to each group’s results. If freezing of surgically-retrieved specimens provides results similar to those with the use of fresh sperm, then the use of freezing specimens would be preferable. If not, fresh specimens are preferable.
The reproductive potential of infertile men undergoing ART is related to the type of azoospermia. According to our data, the chances of retrieving spermatozoa (odds ratio [OR] = 43.0; 95% confidence interval [CI]: 10.3-179.5) and of achieving a live birth by ICSI (OR=1.86; 95% CI:l 1.03-2.89) were significantly increased in couples whose male partner had obstructive rather than non-obstructive azoospermia. Children conceived using sperm retrieved from men with OA and NOA should be followed-up because it is still unclear if there is an increased risk of birth defects when ICSI is carried out with non-ejaculated sperm.
References
Esteves SC, Glina S. Recovery of spermatogenesis after microsurgical subinguinal varicocele repair in azoospermic men based on testicular histology. IntBraz J Urol. 2005; 31:541-8.
Verza S Jr, Esteves SC. Sperm defect severity rather than sperm source is associated with lower fertilization rates after intracytoplasmic sperm injection. IntBraz J Urol. 2008,34:49-56.
Esteves SC, Verza S, Prudencio C, Seol B. Sperm retrieval rates (SRR) in nonobstructive azoospermia (NOA) are related to testicular histopathology results but not to the etiology of azoospermia. FertilSteril. 2010; 94(Suppl.):S132.
Esteves SC, Verza S, Prudencio C, Seol B. Success of percutaneous sperm retrieval and i
The 2nd Gulf Andrology Conference
Riyadh Military Hospital, Ministry of Defense
Riyadh, Saudi Arabia, March 3-4, 2012
Lectures: Current and Future Treatments for Azoospermia
Dr. Kenneth Dickie from Royal Centre of Plastic Surgery in Barrie, Ontario explained the use of stem cells technology in plastic surgery.
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Clinical management of men with nonobstructive azoospermia - Sperm Retrieval ...Sandro Esteves
Reproductive Andrology Workshop III
17-21 January 2016 - Kuwait City - KUWAIT
Organized by: Al Jahra Reproductive Medicine Unit - Ministry of Health
Lecture 4: Sperm Retrieval Methods in Nonobstructive Azoospermia
Pregnancy outcome following swim up preparation of both fresh and cryopreserv...lukeman Joseph Ade shittu
This study was designed to assess the impact of swim up preparation of both fresh and cryopreserved sperm on the pregnancy outcome in a private fertility centre in Lagos. A cross-sectional prospective analysis of 34 asthenozoospermic semen samples of men whose wives were undergoing assisted reproduction was studied. The basic semen parameters comprising of the volume, count, and motility of the sperm before and after swim up preparations with pregnancy outcome were measured. For fresh semen (n = 28, mean age = 37.0 ± 1.1 years, mean volume = 2.16 ± 0.1 ml), the sperm count decreased significantly (p<0.01)><0.01)><0.01)><0.01) from 25.1 ± 4.01 to 32.8 ± 6.18%. The pregnancy outcome of cryopreserved was 30%. The pregnancy outcome was higher with fresh than the cryopreserved semen. However, the motility was a significant indicator for the successful outcome. Swim up procedure improve the motility of both cryopreserved and fresh semen with a better pregnancy outcome in this study.
1. Discuss normal vs. abnormal semen analysis
2. Evaluate different treatments of varicocele
3. Assess azoospermia and discuss micro dissection testicular sperm extraction
4. Diagnose Klinefelter syndrome and genetic abnormalities in men with infertility
ADVANCED SPERM PREPARATION TECHNIQUES.pptxRahul Sen
IN IVF SPERM PREPARATION PLAYS AN IMPORTANT ROLE AS EMBRYO OUTCOMES IS 50% DEPENDENT ON ONE OF THE MAJOR GAMETE THAT IS SPERM. SPERMS POST EJACULATE SHOULD BE HANDLED PROPERLY AND MUST BE SEPARATED FROM SEMINAL PLASMA WELL ENOUGH THAT ONLY MORPHOLOGICALLY MOTILE HEALTHY SPERMS ARE AVAILABLE FOR FERTILIZATION. OVER MANY YEARS CONVENTIONAL METHODS OF SPERM PREPARATIONS WERE CONSIDERED IN ROUTINE, MANY ADVANCED METHODS ARE STILL NOT EXPLORED WHICH HOLDS MORE EFFICACY OVER CONVENTIONAL METHODS OF SPERM PREPARATION.
Invited lecture by Dr Sujoy Dasgupta on "Azoospermia - Evaluation and Management" in a CME on "Standardising Male Factor Evaluation" organised by Indian Fertility Society (IFS) on 20 January 2024.
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Sperm Retreival: Optimizing Sperm Retrieval and Pregnancy in Nonobstructive Azoospermia
1. Optimizing Sperm Retrieval
Paul J. Turek M.D.
Director, The Turek Clinic,
Beverly Hills and San Francisco, CA
1
2
3
654
9
8
7
11
10
12
13
14
171615
20
19
18
22
21
R L
2. Optimizing Sperm Retrieval
Learning Objectives
• Describe the role of medical therapy before sperm retrieval
• Delineate two strategies currently used to find and retrieve
testicular sperm in nonobstructive azoospermia
• Explain how sperm search can be optimized in the andrology
laboratory
6. • N=42
azoospermic
men
treated
with
clomid:
64%
ejaculated
sperm!
Hussein
et
al
J.
Androl.
26:
787,
2005
Medical Therapy May Help
Shiraishi
et
al,
Hum
Reprod.
2012,
27:331-‐9
N=48 failed mTESE
No Rx
+ Rx
7.
8. Raman and Schlegel. J Urol. 2002 167:124
Medical Therapy May Help
Motile ejaculated sperm
after varicocelectomy
N=233
Overall success=39%
Weedin et al. J Urol. 2010, 183; 2309
12. 2010-2012
N=40 men with
cryptozoospermia
Mean age=38 years
No sperm
Nonmotile
Motile
Motile and
nonmotile
10%
25%
60%
5%
Hagerty et al, 2012
• 85% of men able to
bank sperm
• 40% sample-to-
sample
variability
“Epidemiology” of
Cryptozoospermia
13. • 2010-2012
• N=40 men with
cryptozoospermia
• To date, 13/40 couples to IVF-ICSI
• Sperm source:
Fresh
Ejaculate
77%
Thawed
Ejaculate
8%
mTESE
15%
• Mean partner age 32 (27, 41)yr
• 60% 2PN rate
• 46% (6/13) ongoing preg. rate
Hagerty et al, 2012
“Epidemiology” of
Cryptozoospermia
14. Sperm Source and ICSI Fertilization
NOA-Testis sperm
NOA-Cryptozoospermia
X
X
What kind of performance differences do we expect
between cryptozoospermic and testicular sperm?
17. Principle: Primum no nocere
Least invasive, least damaging, best yield.
Turek et al. Ass Reprod Rev. 1999, 9: 60-64
Guiding Principle
18. Epididymal Sperm: Evidence-Based Guidelines
Nicopoullos et al. Fert Steril. 2004, 82: 691-701
Donoso, Tournaye, Devroey. Hum Reprod Upd. 2007, 13: 539-549
Van Peperstraten et al. Cochrane Database Syst Rev. 2006, 3:CD 002807
• For epididymal vs. testicular sperm in obstructive
azoospermia:
Insufficient evidence to detect a difference in
outcome.
• For fresh vs. frozen thawed epididymal sperm:
There is no difference in clinical outcome (FR,
OPR)
20. Testis Sperm: Evidence-Based Guidelines
Donoso, Tournaye, Devroey. Hum Reprod Upd. 2007, 13: 539-549
Van Peperstraten et al. Cochrane Database Syst Rev. 2006, 3:CD 002807
Nicopoullos et al. Fert Steril. 2004, 82: 691-701
• For obstructive vs. nonobstructive azoospermia:
Insufficient evidence to recommend one
sperm retrieval technique over another.
• Sperm retrieval in cases of nonobstructive azoospermia
Can be very difficult due to “patchy” or “focal”
nature of production.
• In cases of nonobstructive azoospermia:
There is no relationship between the sperm
technique chosen and ICSI outcomes.
21. What About “Delayed Fresh” Sperm Retrieval?
Morris et al. J Urol. 2007, 178:2087-91
0%
5%
10%
15%
20%
25%
Initial 24 hours 48 hours
OA (n=51)
NOA (n=44)
Time After Sperm Retrieval
% Motility
Testis sperm motility
22. Study of Motility and Viability of Aspirated Sperm
Bachtell et al. Hum Reprod. 1999, 14:101
Motility *Viability
Fresh Thawed Fresh Thawed
Testis, NOA 5% 0.2% 86% 46%
Epididymis, OA 22% 7% 57% 24%
Vas deferens, fertile 71% 38% 91% 51%
*Vital Stains: carboxyfluorescein, 0.08mg/mL;
propidium iodide, 20mg/mL
28. How is FNA Mapping Different from Microdissection?
Office FNA Map
Sperm Found?
Yes No
IVF/ICSI
"Directed" TESE
Donor Sperm
Adoption
FNA Mapping
Microdissection
TESE in OR
Sperm Found?
IVF/ICSI
Yes No
Donor Sperm
Adoption
+/- Pregn
Microdissection
34. Optimizing Sperm Retrieval
Patient Procedure Laboratory
Medical therapy
Cryptozoospermia
TESA/TESE
Microdissection
FNA Mapping
Search time
Sperm banking
35. Laboratory Effort in Sperm Retrieval Cases
Turek PJ. Sperm Retrieval Techniques. In: The Practice of Reproductive
Endocrinology and Infertility: The Practical Clinic and Laboratory. Ed. D.
Carrell. 2010.
Procedure MESA TESA TESE MicroTESE
Man-hrs 1hr 1-2hrs 2-4 hrs 4-6 hrs
needed
36. Single sperm cryopreservation on cryoloops: an
alternative to hamster zona for cryopreservation of
individual spermatozoa
Nina Desai, Heather Blackmon, James Goldfarb.
Fertil Steril, Vol 80, Suppl. 3, 2003, pp, 55-56
37. Optimizing Sperm Retrieval
Summary
• Patients really only want one sperm retrieval.
• They like their testosterone levels where they are.
• You have one good shot at them.
• So, optimize medical therapy, surgical technique
and laboratory variables for each case.