This document compares BEROTM biosurfactant technology to other enhanced oil recovery methods such as acidification, polymer flooding, fracturing, and microbial enhanced oil recovery technologies. It finds that BEROTM has higher success rates, recovery effects, and effectiveness than other methods, does not cause pollution, and requires no maintenance. It also details how BEROTM specifically targets reservoirs to reduce interfacial tension and remove plugging, making it more efficient and stable than endogenous microbial recovery technologies. The document concludes that BEROTM biosurfactant is the best global method for improving oil recovery rates and solving pollution problems during oil production.

1. www.zfatech.com
UNITED STATES
Add: 12212 Ashling Dr Stafford, TX, 77477
Tel: +1713 893 4784
CHINA
Add:Nanopolis Suzhou: NW02, Room 110,
99 JinjiLake Avenue,Jiangsu Province, P.R. China.
Zip: 215123
Tel: +86 512-6999 5236 Fax: +86 512-6999 5237
E-mail: contact@zfatech.com
Difference between BERO™ Biosurfactant Method and Other Enhanced
Oil Recovery Methods
Comparison between BERO™ biosurfactant technology and technologies
such as acidification, fracturing and polymer flooding:
As for conventional technologies such as acidification, polymer flooding and
fracturing, due to their own limitations, they cannot achieve ideal effect or only
have short-term effect and may cause secondary pollution of reservoir. For
instance, if acidification is adopted to remove plug, high acid concentration is
required, effective action radius is often small and substances dissolved by
acid may cause new plug, so it only has short-term effect. This method
possibly results in damage of reservoir and environment and corrosion of
equipment. As for fracturing, it can only act on fractures and will fast increase
water cut. As for polymer flooding, produced wastewater not only has high oil
content, but also contains a lot of polymer, thus increasing liquid viscosity,
improving the oil carrying capability of liquid and also increasing difficulty in
water-oil separation.
Acidification Polymer Chemistry Water Injection Bacterial BERO™
Success Rate 80% 80% 90% 80% 90% UP
Recovery
Effect
40% 50% 70% 20-100% 120% UP
Effectiveness 3 m 3 m 12 m 3-6 m 12 m
Side Effect
Pollution of
water and soil,
secondary
scaling
Pollution of water and
soil, secondary
scaling
Use large water,
formation water
pollution;
secondary scaling
Biodegradable, no-
polluting, but
requires ongoing
maintenance
Biodegradable,no
polluting, no
maintenance

2. www.zfatech.com
UNITED STATES
Add: 12212 Ashling Dr Stafford, TX, 77477
Tel: +1713 893 4784
CHINA
Add:Nanopolis Suzhou: NW02, Room 110,
99 JinjiLake Avenue,Jiangsu Province, P.R. China.
Zip: 215123
Tel: +86 512-6999 5236 Fax: +86 512-6999 5237
E-mail: contact@zfatech.com
Acidizing
Techniques
Polymer Water Injection
Bacteria
technology
BERO™
Success Ratio 80% 80% 90% 80% 90% and above
Increase Effect 40% 50% 70% 20-100% 120% and above
Utility cycle 3 months 3 months 12 months 3-6 months 12 months
Side Effect
Pollution of
water and
soil;The
secondary
scale
Pollution of
water and
soil;The
secondary
scale
Huge water
consumption;
Formation water
pollution; The
secondary scale
Biodegradation, no
pollution, but the
need for nutrient
solution was
injected to foster
Biodegradable,No
pollution;No
maintenance cost
Compared with the above methods, BERO™ biosurfactant technology
prevents their disadvantages perfectly:

3. www.zfatech.com
UNITED STATES
Add: 12212 Ashling Dr Stafford, TX, 77477
Tel: +1713 893 4784
CHINA
Add:Nanopolis Suzhou: NW02, Room 110,
99 JinjiLake Avenue,Jiangsu Province, P.R. China.
Zip: 215123
Tel: +86 512-6999 5236 Fax: +86 512-6999 5237
E-mail: contact@zfatech.com
(I) It can work under the condition of low concentration (0.5%); it has the same
density as water and is hydrophilic; it can diffuse through penetration while it is
pushed into formation, so it has larger action radius;
(II) It can be firmly adsorbed on rock surface and keep rock surface wettable
so as to prevent wax plugging and maintain plugging removal effect;
(III) It can completely release oil from rock surface, increase the relative
penetrability of oil so as to lower water cut;
(IV) It is a non-acidified preparation and will not cause secondary pollution of
reservoir or disturb crude oil dehydration and sewage treatment systems and
can prevent secondary plugging since it produces no other new product during
reaction;
(V) It is biologically degradable, will not corrode or pollute facilities (such as
pipes) and surrounding environment or do harm to human or animals, so it is
an environmentally friendly product;
(VI) This product totally meets the HSE requirements for working environment

4. www.zfatech.com
UNITED STATES
Add: 12212 Ashling Dr Stafford, TX, 77477
Tel: +1713 893 4784
CHINA
Add:Nanopolis Suzhou: NW02, Room 110,
99 JinjiLake Avenue,Jiangsu Province, P.R. China.
Zip: 215123
Tel: +86 512-6999 5236 Fax: +86 512-6999 5237
E-mail: contact@zfatech.com
during oilfield development.
Comparison between BERO™ biosurfactant technology and other
microbial enhanced oil recovery:
Microbial enhanced oil recovery (hereinafter referred to as MEOR) is a green
and environmentally friendly technology having high technology content and
rapidly developing in the field of enhanced oil recovery and oil-related
environmental protection at home and abroad. At present, due to the import of
high-tech technologies and the influence of concept ambiguity of oilfield
service providers at home and abroad, MEOR is commonly considered as
endogenous (heterologous) microbial enhanced oil recovery which has been
researched and tested for many years in China (such as MEOR mentioned in
“Petroleum Science” at the website of CNPC). In fact, to the knowledge of
international enhanced oil recovery industry, MEOR is the general name for
biological enhanced oil recovery technologies and it can be divided by
mechanisms as follows: endogenous (heterologous) microbial enhanced oil
recovery (i.e. bacteria process) and enhanced oil recovery using bio-enzyme.
Enzyme 1
(US company)
Enzyme 2
(Norway)
BERO™
Success Rate 80% 90% 90%up
Oil Gain % 50% 20% ≥120%
Effective period 3-6 months 12 months 12 months up
Stability No Yes Yes
Application IFT Reduction
IFT Reduction
Reservoir Plugging
Removal
IFT Reduction
Reservoir Plugging
Removal
Max Temperature 70°C 180°C 220°C
Endogenous (heterologous) technology utilizes the product produced from the
reaction of bacteria to conduct enhanced oil recovery and inevitably has the
disadvantages that the selection, survival, adaptability, reproduction, reaction,
mutation and degradation and the like of bacteria which are complicated and
easily affected by uncertainty and have slow effect. BERO™ biosurfactant
technology utilizes bio-enzyme to directly act on the target and enzyme is an

5. www.zfatech.com
UNITED STATES
Add: 12212 Ashling Dr Stafford, TX, 77477
Tel: +1713 893 4784
CHINA
Add:Nanopolis Suzhou: NW02, Room 110,
99 JinjiLake Avenue,Jiangsu Province, P.R. China.
Zip: 215123
Tel: +86 512-6999 5236 Fax: +86 512-6999 5237
E-mail: contact@zfatech.com
internationally recognized efficient, stable, mild and specific microorganism
and can specifically facilitate a certain reaction without participating in the
reaction and allow high reaction efficiency, mild reaction condition, little
pollution caused by reaction product, low energy consumption and easily
controlled reaction. Therefore, BERO™ biosurfactant can reduce oil content of
mud by about 0.20% within 60 seconds, will not be affected by temperature,
pressure, acid, alkali and water salinity and has strong adaptability and
controllability. It can be firmly adsorbed on rock surface and keep rock surface
wettable for a long time, thus maintaining long-term plugging removal effect;
after reaction, BERO™ biosurfactant is reduced to original state. BERO™
biosurfactant technology has inherent advantages, provides high efficiency
and stability, strong adaptability and fast effect and has long lasting time.
In addition, Endogenous (heterologous) technology requires a lot of analysis
and study at the earlier stage of construction and input of a lot of labor force to
conduct monitoring and research after construction, and also requires periodic
supplementation of nutrient solution and gas and cannot be easily understood
by production managers who are not familiar with biological methods. However,
BERO™ biosurfactant technology only needs the existing data at the earlier
stage of construction; it can develop construction plan without a lot of extra
experiments; it has simple process which does not require a lot of labor force
and materials during construction; it does not need any subsequent work after
construction; it is easily accepted by oilfield production personnel and has
strong operability.
To sum up, BERO™ biosurfactant is used for improving oil recovery rate and
solving pollution problem during oilfield production and is the best method for
stimulation and improving oil recovery rate in the world. It will enable enhanced
oil recovery to make a revolutionary process.