bero™ biosurfactant technology introduction 8

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1) Prepare liquid: V=лr2•h•Φ Where:V—Dosage of BERO™ biosurfactant, expressed in m3 л—3.14 r—Treatment radius of production well, expressed in m h—Effectively thickness of reservoir, expressed in m Φ—Effective porosity of reservoir, expressed in % 2) Pull out production string, set tubing to the downhole and flush well with large displacement. 3) Set construction string (sealing test must be conducted on packer (if any)). 4) Squeeze BERO™ biosurfactant.

Technology

www.zfatech.com
UNITED STATES
Add: 12212 Ashling Dr Stafford, TX, 77477
Tel: +1713 893 4784
CHINA
Add:Nanopolis Suzhou: NW02, Room 110,
99 JinjiLake Avenue,Jiangsu Province, P.R. China.
Zip: 215123
Tel: +86 512-6999 5236 Fax: +86 512-6999 5237
E-mail: contact@zfatech.com
1) Prepare liquid: V=лr2·h·Φ
Where:V—Dosage of BERO™ biosurfactant, expressed in m3
л—3.14
r—Treatment radius of production well, expressed in m
h—Effectively thickness of reservoir, expressed in m
Φ—Effective porosity of reservoir, expressed in %
2) Pull out production string, set tubing to the downhole and flush well with
large displacement.
3) Set construction string (sealing test must be conducted on packer (if any)).
4) Squeeze BERO™ biosurfactant.
5) Squeeze displacement fluid and push BERO™ biosurfactant into the target
zone.
6) Soaking period for 3-5 days.
7) Pull out construction string.
8) Set production string according to planning requirement and resume

（EOR/MEOR/BERO）After the use of BERO™ biosurfactant, those three wells obtained good stimulation effect and as of the end of February 2010, proven oil production increased by 15,811.2 bbls (5,270.4 bbls per well) and these three wells still have development value. Therefore, it is believed that BERO™ biosurfactant technology will greatly improve the recovery rate and utilization rate of oil well, increase crude oil production and bring remarkable economic benefit.

Reservoir cleaning and oil displacement mechanisms of bero™ biosurfactant

Matt Wu

(EOR/MEOR/BERO/oil recovery) Reservoir cleaning and oil displacement mechanis...

Matt Wu

(EOR/MEOR/BERO/oil recovery) After BERO™ biosurfactant is injected into a water injection well, it has the functions of cleaning, oil displacement, stimulation, facilitating drainage, penetration and biological degradation such as preventing organic deposition, so change happens to the reservoir within the retention zone and the passage of injected water flow also changes when BERO™ biosurfactant evenly diffuses in the reservoir, passages with small resistance causing “short-circuit” are influenced, water flow is forced to search for more new passages and the sweep area of water flooding is enlarged, thereby improving water flooding effect and realizing plugging removal and injection reduction.

Application of bero biosurfactant in pucheng oilfield and its effect

Matt Wu

Application of bero™ biosurfactant in pucheng oilfield and its effect

Matt Wu

Basic data requires for injection design 1) Drilling and completion dates, total depth, return top of cement, cement plug depth, casing size and setting depth, wall thickness, distance between casing (tubing) head and bushing, location of casing collar nearby packer sticking point, maximum well deviation and depth, commissioning (injection starting) date and cementing quality 2) Perforated horizon, perforated interval, actual perforated horizon, actual perforated interval, thickness, number of layers and perforation parameters (gun type, density, porosity, penetrability and mud content)

bero™ biosurfactant technology introduction 5

Matt Wu

BERO™ biosurfactant technology can not only greatly improve oil recovery rate, but also greatly reduce water injection and water cut of produced liquid, lower energy and machinery consumption, improve the efficiency of production well systems and prolong workover-free period. The production of crude oil greatly increases while production cost is lowered, so remarkable economic benefit is gained.

bero™ biosurfactant technology introduction 4

Matt Wu

After BERO™ biosurfactant is injected into a water injection well, it has the functions of cleaning, oil displacement, stimulation, facilitating drainage, penetration and biological degradation such as preventing organic deposition, so change happens to the reservoir within the retention zone and the passage of injected water flow also changes when BERO™ biosurfactant evenly diffuses in the reservoir, passages with small resistance causing “short-circuit” are influenced, water flow is forced to search for more new passages and the sweep area of water flooding is enlarged, thereby improving water flooding effect and realizing plugging removal and injection reduction.

bero™ biosurfactant technology introduction 2

Matt Wu

BERO™ biosurfactant technology introduction 1

Matt Wu

BERO™ biosurfactant is a polymeric biological enzyme and as a water-soluble product, it can efficiently release hydrocarbon on the surfaces of solid particles in reservoir. After being injected into the formation, due to activity, it can adhere to rock surface for a long time to change the property of rock formation into hydrophilic biological surface, reduce wetting angle and the interfacial tension of rock particles in reservoir, thereby reducing the flowing resistance of crude oil in reservoir pores; meanwhile, due to biological degradation effect, BERO™ biosurfactant will continuously deform and split oil drops until oil drops are released from rock formation, thus not only being able to loosen the crude oil of retention layer, but also making oil drops become smaller and more easily exude through the pores of rock formation until they are recovered. Since enzyme will form a kind of mild emulsion composed of oil, water, enzyme, enzyme-based ferment, etc. The emulsion can resist against, contact and change the retention layer, thereby achieving the effect of cleaning, injection reducing and oil displacement and improving oil recovery rate. Meanwhile, it will not produce other new product, so it will not plug pores or cause secondary pollution.

Application of bero™ biosurfactant in zhiluo oilfield and its effect(4)

Matt Wu

Application of bero™ biosurfactant in zhiluo oilfield and its effect(3)

Matt Wu

Application of bero™ biosurfactant in xing zichuan oilfield and its effect(6)

Matt Wu

Application of bero™ biosurfactant in daqing and its effect(1)

Matt Wu

(EOR/MEOR/BERO/oil recovery) Material safety data sheet

Matt Wu

（EOR/MEOR/BERO/oil recovery）Animal acute toxicity test

Matt Wu

（EOR/MEOR/BERO/oil recovery）After feed with enzyme solution, mice slightly quiet for a few moments and resume to normal activities, animals without acute death. We continue to observe mice but still no mice dead after seven days. No abnormal secretions from mice’s natural holes and activity, diet, color were normal, also no abnormal changes for mice’s feces and urine within 7days; the weight is 27.55±3.6g, net weight is 7.95g within seven days. Put the mice to death after 7days; observe the heart, liver, lung, spleen, kidney, gastrointestinal and other organs showed no abnormality. The maximum dose of the test gavage is 10.20g/kg still cannot cause the mice to death. This explains the maximum tolerated dose (LD0) is greater than 10g/kg. According to the acute toxicity test of acute toxicity classification GB15193.3-94, this enzyme should be nontoxic.

(EOR/MEOR/BERO/oil recovery) Bero™ biosurfactant：laboratory experiment report...

Matt Wu

(EOR/MEOR/BERO/oil recovery) Bero™ biosurfactant：laboratory experiment report...

Matt Wu

(EOR/MEOR/BERO/oil recovery) Application of bero™ biosurfactant in yanchang o...

Matt Wu

(EOR/MEOR/BERO/oil recovery) As for Well Zhang 279 Cong 1, the analysis result shows that the main reason for production reduction is the plugging of the near-wellbore zone caused by organic matters such as wax and asphalt contained in crude oil. In September 2012, BERO™ biosurfactant was mixed with formation water and injected in Well Zhang 279 Cong 1 to remove plugging and stimulate production and the temperature of the injection fluid was above 122℉. During construction, pressure rose from 0 psi to 1450 psi. After shut-in for 5 days, Well Zhang 279 Cong 1 resumed production and then its production greatly increased. Its daily oil production increased from 11.7 BOPD to 17.4 BOPD.

(EOR/MEOR/BERO/oil recovery) Application of bero™ biosurfactant in qinghai oi...

Matt Wu

(EOR/MEOR/BERO/oil recovery) According to the effect and characteristics of BERO™ biosurfactant and the production need and geological solution of Well Wu 11-7 of Qinghai Oilfield, we carried out the plug removal of this well by using BERO™ biosurfactant in March 2006 and then this well was shut in for 5 days; its daily oil production reached 50.4 BOPD within 7 days after this well resumed production, maintained at about 8.6 BOPD from April 2006 to January 2007 and reached 2.2 BOPD from January 2007 to April 2007. So far, oil production increases by about 2,779 bbls (1608%) on a cumulative basis.

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bero™ biosurfactant technology introduction 8

1. www.zfatech.com UNITED STATES Add: 12212 Ashling Dr Stafford, TX, 77477 Tel: +1713 893 4784 CHINA Add:Nanopolis Suzhou: NW02, Room 110, 99 JinjiLake Avenue,Jiangsu Province, P.R. China. Zip: 215123 Tel: +86 512-6999 5236 Fax: +86 512-6999 5237 E-mail: contact@zfatech.com 1) Prepare liquid: V=лr2·h·Φ Where:V—Dosage of BERO™ biosurfactant, expressed in m3 л—3.14 r—Treatment radius of production well, expressed in m h—Effectively thickness of reservoir, expressed in m Φ—Effective porosity of reservoir, expressed in % 2) Pull out production string, set tubing to the downhole and flush well with large displacement. 3) Set construction string (sealing test must be conducted on packer (if any)). 4) Squeeze BERO™ biosurfactant. 5) Squeeze displacement fluid and push BERO™ biosurfactant into the target zone. 6) Soaking period for 3-5 days. 7) Pull out construction string. 8) Set production string according to planning requirement and resume

2. www.zfatech.com UNITED STATES Add: 12212 Ashling Dr Stafford, TX, 77477 Tel: +1713 893 4784 CHINA Add:Nanopolis Suzhou: NW02, Room 110, 99 JinjiLake Avenue,Jiangsu Province, P.R. China. Zip: 215123 Tel: +86 512-6999 5236 Fax: +86 512-6999 5237 E-mail: contact@zfatech.com production. Description: Only backflow is needed without moving the pipe string if such operation as pump inspection is not necessary in production wells. Then the following steps will be fine: Step 1, Step 4, Step 5, Step 6 and Step 8.

bero™ biosurfactant technology introduction 8

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