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Talos Arctica
Resolve 3D macromolecular structures quickly, efficiently, and accurately
The Talos Arctica™
is a 200 kV FEG
Transmission and Scanning
Transmission Electron Microscope
(S/TEM). It is a powerful, stable, and
highly automated system optimized
for high-resolution 3D imaging of
proteins and macromolecular
assemblies as well as high-resolution
cellular tomography.
With its innovative design to increase throughput, stability
and ease of use, the Talos Arctica enables scientists to quickly
obtain better insight and understanding of macromolecular
structures, cellular components, cells and tissues in three
dimensions. Its revolutionary cryo-based technology and
stability permits a full range of semi-automated applications
including: single particle analysis, electron crystallography
and cryo electron tomography.
Shorter time to high quality data
The platform design combines an excellent optical
performance and thermal and mechanical stability with a
unique, automated, high throughput (cryo) sample loader/
unloader system for multiple sample handling at liquid
nitrogen temperatures.
The system’s Constant-Power™ C-TWIN objective lens
delivers outstanding optical performance to help ensure an
optimal balance of contrast and resolution. FEI Talos’
ultra-stable platform includes a Piezo-enhanced stage
(optionally available at additional cost), stable optics and a
rugged system enclosure for maximum thermal and
mechanical stability.
KEY BENEFITS
Increased data acquisition speed: Acquisition speeds have
been improved up to 3 times for tomography and up to 1.5
times for single particle analysis when EPU is used compared
to side entry loading systems
High data throughput: Robotic sample handling and
automated loading for up to 12 samples enhances up to 40%
in data throughput
Seamless integration: The Talos Arctica is fully compatible
with FEI’s applications SW, Phase Plate and detector solutions
within FEI’s integrated cryo electron microscopy workflow
Unattended operation: Autofilling of liquid nitrogen and
powerful applications SW allows for continuous unattended
platform operation and automated data acquisition
Excellent data quality: Optimized for 80-200 kV operation,
equipped with a constant power C-TWIN objective lens for
optimal contrast/resolution balance
Sample quality: Contamination free sample loading and
automated liquid nitrogen filling results in an increased
sample life time and quality
Low cost of ownership: New design allows for remote
diagnostics and preventive service, lowering ownership cost
and maximizing systems uptime
2 FEI Explore. Discover. Resolve.
User friendly for everyone
The Talos Arctica is a fully digital S/TEM, which incorporates
a new digital search-and-view camera (SmartCam), specially
designed by FEI. This high-speed digital camera replaces the
conventional fluorescent viewing screen and gives users
optional freedom to operate the microscope from a distance.
The Talos is designed with local remote operation capability
as standard. This allows for operation from within the
microscope room, or from an adjacent room which is a
maximum of 10 m away from the computer.
Talos Arctica features the FEI proprietary Velox™ software for
easy-to-use STEM acquisition and analysis utilizing the
Windows 7 operating system. In the unique Talos concept, all
microscope components, like the electron gun, the optical
elements, the vacuum system and the stage, are digitally
controlled. The task-oriented user interface allows users to
automatically recall all optimized operating conditions
including lens settings, gun parameters, optical alignments,
and aperture alignments for all the different techniques such
as TEM, STEM, and low-dose. Likewise, all detectors, such as
the Falcon camera, and STEM detectors, are computer
controlled. The acquisition of data with these detectors is
embedded in the Talos user interface and can be automated
for certain acquisition processes, such as TEM / STEM
tomography data acquisition and EPU, single particle data
acquisition. Because of this sophisticated computer system,
the Talos is especially suited for multi-user and multi-
disciplinary environments and therefore also suitable for
newly emerging customer groups who have their background
in adjacent applications like crystallography.
Ready for the future
The Talos Arctica applications dedication ensures that you
will be able to perform today’s experiments and as well as
address new research problems of the future.
Talos Arctica Technical Features
·· C-TWIN objective lens (Cc and Cs 2.7 mm), ensuring a
good optimum for tilt range, resolution, and contrast
·· Autoloader ensures automated and contamination-free
loading of multiple samples
·· Embedded liquid nitrogen AutoFill system enabling
long-term cryo stability
·· Ultra-high sample stability and drift performance due to
integrated low temperature sample holder design
·· Vitreous ice specimen quality guaranteed for 24 hr in the
microscope, due to oil-free vacuum system and fixed cryo
box surrounding the sample area
·· Full integration with FEI’s single particle and tomography
solutions
·· SmartCam digital search-and-view camera improves the
handling of all applications
·· Workstation including two 24" widescreen LCD monitors
and table improves the operator comfort and allows relaxed
working in an ergonomic environment
·· Automatic aperture system in combination with the
SmartCam prepares Talos Arctica for full remote operation
Explore. Discover. Resolve. FEI 3
TEST SPECIFICATION COMMENTS
X-Ray Safety X-ray radiation ≤ 1 μSv/hr at 0.1 m
distance
Safety test, performed with X-ray sensor.
FEG Probe Current Probe current at 1 nm spot size
X-FEG: > 1.2 nA
The probe current specification ensures
sufficient brightness of the FEG.
Information Limit ≤ 0.23 nm at zero specimen tilt
≤ 0.34 nm at -70° and +70° specimen tilt
Young's fringe images showing TEM
information limit.
Thon Rings Thon rings visible beyond a spatial
frequency of 2.7 nm-1 (corresponding to
0.37 nm resolution) in the rotationally
averaged power spectrum of an image of
a PtIr specimen at -2 μm defocus.
Thon rings demonstrate the transfer of
contrast by the microscope at a defocus
value that is commonly used for single
particle data acquisition. An additional
test is performed at -1 μm defocus for
reference only (specification must be met
at -2 μm).
Drift after Specimen Exchange Maximum drift values after exchange
5 min: 1.2 nm/s (LM Overview)
15 min: 0.45 nm/s (Target Areas)
30 min: 0.25 nm/s (Start Tomo)
60 min: 0.05 nm/s (Start EPU)
Drift measurement (5 minute running
average) performed immediately after
specimen exchange, demonstrating the
behavior under normal use conditions.
The drift values correspond to typical
use-case requirements.
Autoloader Performance The following procedure should run
without errors: each of the 4 cassettes is
filled with 6 AutoGrids and docked. Next,
an inventory is made and each AutoGrid
is loaded into the column once.
Performance test, demonstrating the full
load/unload cycle and proving the
compatibility of the autoloader parts
delivered with the system. Slot positions
1 to 12 of the cassettes are alternately
filled and empty.
STEM resolution (Option) < 0.23 nm Lattice reflections visible in power
spectra of images acquired in STEM
mode on a gold on carbon specimen.
Tomography (Option) Reproducibility X, Y ≤ 0.4 um during
Alpha tilt from -70° to +70°
Eucentricity X, Y ≤ 2 um during Alpha tilt
from -70° to +70°
Eucentricity defocus ≤ 4 um during Alpha
tilt from -70° to +70°
Calibrations including auto-functions,
holder calibration with evaluation of
3-holder measurement and Tomography
specific calibrations.
EPU (Option) Sample data set of 15 images,
demonstrating that the proper target
areas were imaged.
EPU is set up to acquire 3 images of the
edges of 5 different foil holes. The
orientation of the edges in the acquired
data set demonstrates that the target
areas were imaged properly.
For current certifications, please visit FEI.com/certifications
©2014. We are constantly improving the performance of our products—all specifications are subject to change without notice.
FEI, the FEI logo, Constant Pwer, Talos Arctica, and Velox are trademarks of FEI Company or its affiliates. All other trademarks belong to their
respective owners. DS0189-10-2014
World Headquarters
Phone +1 503 726 7500
FEI Europe
Phone +31 40 23 56000
FEI Japan
Phone +81 3 3740 0970
FEI Asia
Phone +65 6272 0050
FEI Australia
Phone +61 2 6173 6200
Learn more at FEI.com
ContactUs@FEI.com
Notes

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Talos_Arctica_Datasheet

  • 1. Talos Arctica Resolve 3D macromolecular structures quickly, efficiently, and accurately The Talos Arctica™ is a 200 kV FEG Transmission and Scanning Transmission Electron Microscope (S/TEM). It is a powerful, stable, and highly automated system optimized for high-resolution 3D imaging of proteins and macromolecular assemblies as well as high-resolution cellular tomography. With its innovative design to increase throughput, stability and ease of use, the Talos Arctica enables scientists to quickly obtain better insight and understanding of macromolecular structures, cellular components, cells and tissues in three dimensions. Its revolutionary cryo-based technology and stability permits a full range of semi-automated applications including: single particle analysis, electron crystallography and cryo electron tomography. Shorter time to high quality data The platform design combines an excellent optical performance and thermal and mechanical stability with a unique, automated, high throughput (cryo) sample loader/ unloader system for multiple sample handling at liquid nitrogen temperatures. The system’s Constant-Power™ C-TWIN objective lens delivers outstanding optical performance to help ensure an optimal balance of contrast and resolution. FEI Talos’ ultra-stable platform includes a Piezo-enhanced stage (optionally available at additional cost), stable optics and a rugged system enclosure for maximum thermal and mechanical stability. KEY BENEFITS Increased data acquisition speed: Acquisition speeds have been improved up to 3 times for tomography and up to 1.5 times for single particle analysis when EPU is used compared to side entry loading systems High data throughput: Robotic sample handling and automated loading for up to 12 samples enhances up to 40% in data throughput Seamless integration: The Talos Arctica is fully compatible with FEI’s applications SW, Phase Plate and detector solutions within FEI’s integrated cryo electron microscopy workflow Unattended operation: Autofilling of liquid nitrogen and powerful applications SW allows for continuous unattended platform operation and automated data acquisition Excellent data quality: Optimized for 80-200 kV operation, equipped with a constant power C-TWIN objective lens for optimal contrast/resolution balance Sample quality: Contamination free sample loading and automated liquid nitrogen filling results in an increased sample life time and quality Low cost of ownership: New design allows for remote diagnostics and preventive service, lowering ownership cost and maximizing systems uptime
  • 2. 2 FEI Explore. Discover. Resolve. User friendly for everyone The Talos Arctica is a fully digital S/TEM, which incorporates a new digital search-and-view camera (SmartCam), specially designed by FEI. This high-speed digital camera replaces the conventional fluorescent viewing screen and gives users optional freedom to operate the microscope from a distance. The Talos is designed with local remote operation capability as standard. This allows for operation from within the microscope room, or from an adjacent room which is a maximum of 10 m away from the computer. Talos Arctica features the FEI proprietary Velox™ software for easy-to-use STEM acquisition and analysis utilizing the Windows 7 operating system. In the unique Talos concept, all microscope components, like the electron gun, the optical elements, the vacuum system and the stage, are digitally controlled. The task-oriented user interface allows users to automatically recall all optimized operating conditions including lens settings, gun parameters, optical alignments, and aperture alignments for all the different techniques such as TEM, STEM, and low-dose. Likewise, all detectors, such as the Falcon camera, and STEM detectors, are computer controlled. The acquisition of data with these detectors is embedded in the Talos user interface and can be automated for certain acquisition processes, such as TEM / STEM tomography data acquisition and EPU, single particle data acquisition. Because of this sophisticated computer system, the Talos is especially suited for multi-user and multi- disciplinary environments and therefore also suitable for newly emerging customer groups who have their background in adjacent applications like crystallography. Ready for the future The Talos Arctica applications dedication ensures that you will be able to perform today’s experiments and as well as address new research problems of the future. Talos Arctica Technical Features ·· C-TWIN objective lens (Cc and Cs 2.7 mm), ensuring a good optimum for tilt range, resolution, and contrast ·· Autoloader ensures automated and contamination-free loading of multiple samples ·· Embedded liquid nitrogen AutoFill system enabling long-term cryo stability ·· Ultra-high sample stability and drift performance due to integrated low temperature sample holder design ·· Vitreous ice specimen quality guaranteed for 24 hr in the microscope, due to oil-free vacuum system and fixed cryo box surrounding the sample area ·· Full integration with FEI’s single particle and tomography solutions ·· SmartCam digital search-and-view camera improves the handling of all applications ·· Workstation including two 24" widescreen LCD monitors and table improves the operator comfort and allows relaxed working in an ergonomic environment ·· Automatic aperture system in combination with the SmartCam prepares Talos Arctica for full remote operation
  • 3. Explore. Discover. Resolve. FEI 3 TEST SPECIFICATION COMMENTS X-Ray Safety X-ray radiation ≤ 1 μSv/hr at 0.1 m distance Safety test, performed with X-ray sensor. FEG Probe Current Probe current at 1 nm spot size X-FEG: > 1.2 nA The probe current specification ensures sufficient brightness of the FEG. Information Limit ≤ 0.23 nm at zero specimen tilt ≤ 0.34 nm at -70° and +70° specimen tilt Young's fringe images showing TEM information limit. Thon Rings Thon rings visible beyond a spatial frequency of 2.7 nm-1 (corresponding to 0.37 nm resolution) in the rotationally averaged power spectrum of an image of a PtIr specimen at -2 μm defocus. Thon rings demonstrate the transfer of contrast by the microscope at a defocus value that is commonly used for single particle data acquisition. An additional test is performed at -1 μm defocus for reference only (specification must be met at -2 μm). Drift after Specimen Exchange Maximum drift values after exchange 5 min: 1.2 nm/s (LM Overview) 15 min: 0.45 nm/s (Target Areas) 30 min: 0.25 nm/s (Start Tomo) 60 min: 0.05 nm/s (Start EPU) Drift measurement (5 minute running average) performed immediately after specimen exchange, demonstrating the behavior under normal use conditions. The drift values correspond to typical use-case requirements. Autoloader Performance The following procedure should run without errors: each of the 4 cassettes is filled with 6 AutoGrids and docked. Next, an inventory is made and each AutoGrid is loaded into the column once. Performance test, demonstrating the full load/unload cycle and proving the compatibility of the autoloader parts delivered with the system. Slot positions 1 to 12 of the cassettes are alternately filled and empty. STEM resolution (Option) < 0.23 nm Lattice reflections visible in power spectra of images acquired in STEM mode on a gold on carbon specimen. Tomography (Option) Reproducibility X, Y ≤ 0.4 um during Alpha tilt from -70° to +70° Eucentricity X, Y ≤ 2 um during Alpha tilt from -70° to +70° Eucentricity defocus ≤ 4 um during Alpha tilt from -70° to +70° Calibrations including auto-functions, holder calibration with evaluation of 3-holder measurement and Tomography specific calibrations. EPU (Option) Sample data set of 15 images, demonstrating that the proper target areas were imaged. EPU is set up to acquire 3 images of the edges of 5 different foil holes. The orientation of the edges in the acquired data set demonstrates that the target areas were imaged properly.
  • 4. For current certifications, please visit FEI.com/certifications ©2014. We are constantly improving the performance of our products—all specifications are subject to change without notice. FEI, the FEI logo, Constant Pwer, Talos Arctica, and Velox are trademarks of FEI Company or its affiliates. All other trademarks belong to their respective owners. DS0189-10-2014 World Headquarters Phone +1 503 726 7500 FEI Europe Phone +31 40 23 56000 FEI Japan Phone +81 3 3740 0970 FEI Asia Phone +65 6272 0050 FEI Australia Phone +61 2 6173 6200 Learn more at FEI.com ContactUs@FEI.com Notes