Symposium porto 2013 alfredo garcia fernandez
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1. The document discusses local adaptation in marginal high mountain plant populations. It uses the plant species Silene ciliata as a model to study adaptation in peripheral populations located at high altitudes in Mediterranean mountain ranges. 2. Previous studies on S. ciliata have found evidence of local adaptation through floral asynchrony and drought resistance between central and peripheral populations. 3. The authors propose a new study to further evaluate local adaptation and the importance of peripheral populations by examining population size effects, the influence of different gene flow patterns, and detecting adaptation through differential gene expression analysis. A series of controlled experiments are outlined to address these questions.
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Symposium porto 2013 alfredo garcia fernandez
- 1. Local adaptation in marginal high mountain populations A. García Fernández 1,2, J. Morente 1, C. Lara-Romero 1 , M.L. Rubio 1 & J. M. Iriondo 1 1 Universidad Rey Juan Carlos, Móstoles, Madrid, Spain. 2 Institut Botanic de Barcelona, I.C.U.B.-C.S.I.C., Barcelona, Spain
- 2. Mediterranean mountains: a) Local biodiversity. b) High number of endemics. • Unfavorable periods. • Altitude gradient: a) Temperatures. b) Precipitations. c) Soil properties. d) Vegetal community. Study zone Local adaptation in marginal high mountain populations.
- 3. WinterSpringSummer Study zone Local adaptation in marginal high mountain populations.
- 4. Study zone Local adaptation in marginal high mountain populations. Model species: Silene ciliata: • Mediterranean mountain ranges. • Rear margin distribution. • Facultative outcrosser. • Several levels of ploidy. • Late flowering.
- 5. Intermediate population Low population High population Gimenez-Benavides et al. (2007) New Phyto. Study zone What do we know? Floral asynchrony Local adaptation in marginal high mountain populations.
- 6. Intermediate population Low population High population Study zone What do we know? Floral asynchrony Local adaptation Gimenez-Benavides et al. (2007) Ann. Bot. Local adaptation in marginal high mountain populations.
- 7. Study zone What do we know? Floral asynchrony Local adap. Drought resistance García-Fernández et al. (2012) Plant Bio. Local adaptation in marginal high mountain populations. Fv/Fmphtosynteticstatus Days without water supply Low pop. Inter. pop. High pop.
- 8. Study zone What do we know? Floral asynchrony Local adap. Drought resistance Inbreeding García-Fernández et al. (2012) Oikos Local adaptation in marginal high mountain populations. High Pop. Interm. Pop. Low Pop. Surveys %Survival Self-cross Within-cross Betw-cross
- 9. Study zone What do we know? AdAptA New project: Evaluate the importance of peripheral populations vs. central populations. • Population size. • Genetic diversity. • Gene flow. • Potential adaptation. Local adaptation in marginal high mountain populations. Altitude Ecker et al. (2008) Mol. Eco.
- 10. Study zone What do we know? AdAptA New project: Questions: 1. Is there local adaptation between central and peripherial populations? How important is peripheral population size? Local adaptation in marginal high mountain populations.
- 11. Study zone What do we know? AdAptA New project: Questions: 2. Which gene flow is more benefical for peripheral popualtions, central- peripheral or peripheral-peripheral? Local adaptation in marginal high mountain populations.
- 12. Study zone What do we know? AdAptA New project: Questions: 3. Can local adaptation be detected through differential gene expression? Local adaptation in marginal high mountain populations.
- 13. Study zone What do we know? AdAptA Experiments Experiment 1: Population size effect 1. Generate F1 in greenhouse to avoid maternal effects. 2. Set seeds from manual pollinations, F2. 3. Reciprocal sowing and monitoring. Local adaptation in marginal high mountain populations.
- 14. Study zone What do we know? AdAptA Experiments Experiment 2: Gene flow C-P vs. P-P 1. Generate F1 in greenhouse to avoid maternal effects. 2. Seeds from manual pollinations, F2. All crossings. 3. Reciprocal sowing and monitoring. Local adaptation in marginal high mountain populations.
- 15. Study zone What do we know? AdAptA Experiments Experiment 3: Transcriptome analysis 1. Generate F1 from central and marginal populations. 2. Sow in low altitude peripheral location or similar artificial conditions. 3. Analyze seedling transcriptome, Illumina. Local adaptation in marginal high mountain populations.Blavet et al. (2011) BMC Genomics
- 16. Study zone What do we know? AdAptA Experiments Questions Experiment 3: Transcriptome analysis • Best conditions to grow seedlings? • Next Generation Sequencing Plataform? Local adaptation in marginal high mountain populations. Ex situ Greenhouse In situ Field sowing In situ Jiffy pots
Editor's Notes
- Añadir: Winter Spring Sumer? Tx: Ok, pero pon “summer” Indicación sobre el refugio para que sirva como referencia?
- Facultative outcrosser: para indicar que admite tanto la autopolinización como la polinización cruzada
- Añadir una llave a la derecha que haga mención a la conservación? No es necesario y no hay mucho sitio. Indicar que nos concentramos en las poblaciones periféricas de baja altitud Yo añadiría una diapo después de ésta planteando tres cuestiones: Is there local adaptation between central and peripheral populations? How important is peripheral population size? Which geneflow is more beneficial for peripheral populations, central-peripheral or peripheral-peripheral? Can local adaptation be detected through differential gene expression? De esta manera las 3 diapos siguientes sirven para contestar a estas preguntas
- Añadir una llave a la derecha que haga mención a la conservación? No es necesario y no hay mucho sitio. Indicar que nos concentramos en las poblaciones periféricas de baja altitud Yo añadiría una diapo después de ésta planteando tres cuestiones: Is there local adaptation between central and peripheral populations? How important is peripheral population size? Which geneflow is more beneficial for peripheral populations, central-peripheral or peripheral-peripheral? Can local adaptation be detected through differential gene expression? De esta manera las 3 diapos siguientes sirven para contestar a estas preguntas
- Añadir una llave a la derecha que haga mención a la conservación? No es necesario y no hay mucho sitio. Indicar que nos concentramos en las poblaciones periféricas de baja altitud Yo añadiría una diapo después de ésta planteando tres cuestiones: Is there local adaptation between central and peripheral populations? How important is peripheral population size? Which geneflow is more beneficial for peripheral populations, central-peripheral or peripheral-peripheral? Can local adaptation be detected through differential gene expression? De esta manera las 3 diapos siguientes sirven para contestar a estas preguntas
- Añadir una llave a la derecha que haga mención a la conservación? No es necesario y no hay mucho sitio. Indicar que nos concentramos en las poblaciones periféricas de baja altitud Yo añadiría una diapo después de ésta planteando tres cuestiones: Is there local adaptation between central and peripheral populations? How important is peripheral population size? Which geneflow is more beneficial for peripheral populations, central-peripheral or peripheral-peripheral? Can local adaptation be detected through differential gene expression? De esta manera las 3 diapos siguientes sirven para contestar a estas preguntas
- He pasado el esquema de las siembras al punto 3. Creo que es más oportuno presentarlo ahí. Indicar que se evalúan poblaciones periféricas de tamaño pequeño, mediano y grande.
- Resaltar que se evalúan los resultados de los diferentes tipos de cruzamientos en población pequeña, mediana y grande periférica de baja altitud
- En este caso se analiza el transcriptoma y por tanto los efectos maternos no importan. Habría que indicar que se secuenciarán 12 individuos: 3 centrales + 3 periféricos de tamaño pequeño, mediano y grande. También indicar que el mRNA se extraerá cuando las plántulas empiecen a sufrir la sequía estival
- La opción de los jiffy pots consiste en ponerlos en el campo con las semillas y mantenerlos ahí sin considerar un transplante posterior al medio natural. Eso sí, los jiffy pots se pueden enterrar parcialmente o totalmente en el suelo. Una segunda pregunta sería si consideran necesario sembrar semillas de los mismos orígenes en condiciones ambientales correspondientes a la población central y secuenciar igualmente su transcriptoma. En Chipre me recomendaron hacerlo para diferenciar mejor los genes constitutivos (se expresan siempre) de los facultativos (se expresan solo ante determinadas condiciones ambientales, como sequía).