Background: Mycorrhiza the root
• Symbiotic Fungi
• Crucial link between root system and
• Creates surface area
• Releases enzymes in soil to
to break down nutrients
Egli, S.; Brunner, I. (2011): Mykorrhiza. Eine faszinierende
Lebensgemeinschaft im Wald. 3. Aufl. Merkbl. Prax. 35 8 S
Nitrogen Deposition: Questions?
• Will nitrogen exceed the nutritional needs of the
ecosystem causing a decrease in mycorrhizal
• Studies have shown that ecosystems have been
susceptible to decline in response to nitrogen
deposition. (Bevege, 102)
Arbuscular vs Ecto Mycorrhizae
• Ecto can be seen within the naked eye or
dissecting microscope 10x-50x magnification
• Arbuscular can be seen with high
magnification 200x magnification
• In spite of their profound morphological and
taxonomic differences,some of the effects of
AM fungi and ECM fungi on host physiology
are similar (Tinker, Jones & Durall,1992).
• Mycorrhizal colonization will be measured by directly
collecting Populus grandidentata roots. Both AM and
EM mycorrhizal colonization will be quantified on each
root with a microscope and staining techniques.
• The collection of the roots came from
• Three treatment trees and three control trees
• Control trees were sampled outside buffer
and also on same slope
• Organic horizon depth was used to root
Field Methods cont.
• Tree DBH was taken for trees as well as
• Four Cardinal directions for root tracing
• Tree roots were placed in corresponding test
Lab Methods: Ectomycorrhizal
• Roots were separated from soil.
• Cut into 5 mm sections
• Examined under dissecting microscope
• The portion of ectomycorrhizal root tips were
Bevege DI. 1968. A rapid technique for
clearing tannins and staining intact roots
for detection of mycorrhizas caused by
Endogone spp. and some records of
infection in Australasian plants.
Transactions of the British Mycological
Society 51: 808- 810.
Cripps, C. L. 2001. Mycorrhizae of Aspen
Forests: ecology and potential
application. In: Sustaining Aspen in
Western Landscapes: Proceedings of the
Symposium on Western Aspen Forests,
Grand Junction, CO, June 2000. pp. 285-