Applied biochemistry

1. SUB : APPLIED BIO CHEMISTRY
SUZITHRA.R

2.  Electrophoresis is differential
movement or migration of charged
molecules in solution, with response to
an electrical current.
 Separation of molecules according to
size and/or charge.
 Negatively charged in molecules will
be attracted towards anode.
 Positively charged molecules will
move towards cathode.

3.  The rate migration of the molecule
v=Eq/f
Where,
v=MOLECULES VELOCITY
E=ELECTRIC FIELD STRENGTH
q=MOLECULAR CHARGE
f=FRICTION COEFFICIENT OF
MOLECULE

4. ELECTROPHORESIS
1. Moving Boundary Electrophoresis
2. Zone Electrophoresis

5.  Protein solution is injected into a
quartz U-tube.
 The arms of the U-tube are filled with
buffers.
 When electricity is passed, different
species of protein molecule separate in
to bands of protein.
 Discrete zones does not occur.

6.  Separation principle based on charge
to mass ratio molecules.
 Separation is faster.
 Due to high EOF, the molecules
regardless of the charge, they are
moved.