This study examined the effects of applying different rates of biochar and nitrogen fertilizer on the growth and yield of aerobic rice grown in a clayey Rhodic Ferralsol soil in the Brazilian savannah. The study found that: 1) Biochar applications up to 16 Mg/ha did not negatively influence rice grain yield but higher rates of 32 Mg/ha reduced leaf area index and total shoot dry matter. 2) The optimal nitrogen rate to achieve a grain yield above 3 Mg/ha was 46 kg/ha. 3) Higher biochar and nitrogen rates increased soil ammonium and nitrate availability over the growing season.