Single cell protein (SCP) is a dried form of microorganisms like fungi, bacteria, and algae that is used as a protein source for foods and animal feeds. SCP was developed during wartime to address shortages in conventional protein sources. It has several advantages such as producing high quantities of protein from cheap waste materials through simple fermentation processes. Common challenges include removing nucleic acids from SCP to prevent health issues in humans and developing affordable production methods. SCP is now produced commercially from fungi, algae, yeasts and bacteria through large-scale fermentation and harvesting methods.
Fermented foods like cheese, yogurt, and kefir are produced through microbial fermentation. Microorganisms like bacteria, yeasts, and molds interact with foods biochemically, physically, and biologically to produce the final fermented product. In cheese production, a starter culture is added to pasteurized milk, which is fermented to produce curd. The curd is then drained, cut, scalded, stretched, milled, salted, and ripened to produce cheese. Yogurt is made by inoculating milk with bacterial cultures of Lactobacillus bulgaricus and Streptococcus thermophilus, which ferment the milk sugars to produce lactic acid and cause the milk to thicken
Food Industry of Biotechnology involves preparation of different food items that are used as common part of diet throughout the world.The presentation describes the Industrial preparation of Yogurt.
This document discusses various fermented milk products. It begins by describing milk and the fermentation process. It then provides details on different fermented products like cheese, yogurt, cultured buttermilk, acidophilus milk, and kefir. It explains how each product is produced and the microorganisms involved. Various types of each product are also outlined. The document concludes by discussing the health benefits and uses of these fermented milk products.
Lactic acid can be produced through fermentation by microorganisms. It has various industrial uses, especially in cosmetics, pharmaceuticals, chemicals, food, and medical industries. Lactic acid fermentation occurs in wooden fermenters of 25-125 klt capacity using organisms like Lactobacillus kept at temperatures between 30-50°C depending on the species. The pH is maintained between 5.5-6.5 through additions of calcium carbonate or hydroxide and the fermentation takes 5-10 days to complete. Purification includes filtration, acidification, washing, evaporation and passing through ion exchange resins to obtain 50-60% pure lactic acid.
Dairy products like cheese, yogurt, kefir and acidophilus milk are produced through fermentation.
Cheese is made through lactic acid fermentation of milk using starter cultures like Lactococcus lactis. Yogurt is produced using Streptococcus thermophilus and Lactobacillus delbrueckii subspecies bulgaricus. Kefir uses Lactococcus lactis, Lactobacillus delbrueckii subspecies bulgaricus and yeasts. Acidophilus milk production involves Lactobacillus acidophilus.
These fermented dairy products can deliver health benefits like aiding digestion, lowering cholesterol and potentially reducing cancer risks due to the probiotic
Sauerkraut is a fermented cabbage product with a sour taste made through the lactic acid fermentation of shredded cabbage by lactic acid bacteria such as Leuconostoc mesenteroides and Lactobacillus plantarum. The fermentation process typically takes 4-8 weeks to produce sauerkraut with an acidity level of around 1.7% and beneficial probiotic bacteria. Sauerkraut is high in fiber, vitamins, and minerals and consumption has various health benefits such as supporting digestive health and reducing inflammation.
1. The document discusses the production of lactic acid, glutamic acid, and cheese through fermentation processes. Lactic acid bacteria and fungi are used to produce lactic acid from sugars. Corynebacterium glutamicum is commonly used to produce glutamic acid from glucose through biosynthesis pathways.
2. The production of cheese involves pasteurizing milk, adding bacterial cultures, coagulating the milk with rennet enzyme, separating curd from whey, and ripening the curd through the action of molds and bacteria.
3. Specific microorganisms and fermentation steps are outlined for efficiently producing these three compounds at an industrial scale through microbial fermentation of sugars and carbohydrates.
Fermented foods like cheese, yogurt, and kefir are produced through microbial fermentation. Microorganisms like bacteria, yeasts, and molds interact with foods biochemically, physically, and biologically to produce the final fermented product. In cheese production, a starter culture is added to pasteurized milk, which is fermented to produce curd. The curd is then drained, cut, scalded, stretched, milled, salted, and ripened to produce cheese. Yogurt is made by inoculating milk with bacterial cultures of Lactobacillus bulgaricus and Streptococcus thermophilus, which ferment the milk sugars to produce lactic acid and cause the milk to thicken
Food Industry of Biotechnology involves preparation of different food items that are used as common part of diet throughout the world.The presentation describes the Industrial preparation of Yogurt.
This document discusses various fermented milk products. It begins by describing milk and the fermentation process. It then provides details on different fermented products like cheese, yogurt, cultured buttermilk, acidophilus milk, and kefir. It explains how each product is produced and the microorganisms involved. Various types of each product are also outlined. The document concludes by discussing the health benefits and uses of these fermented milk products.
Lactic acid can be produced through fermentation by microorganisms. It has various industrial uses, especially in cosmetics, pharmaceuticals, chemicals, food, and medical industries. Lactic acid fermentation occurs in wooden fermenters of 25-125 klt capacity using organisms like Lactobacillus kept at temperatures between 30-50°C depending on the species. The pH is maintained between 5.5-6.5 through additions of calcium carbonate or hydroxide and the fermentation takes 5-10 days to complete. Purification includes filtration, acidification, washing, evaporation and passing through ion exchange resins to obtain 50-60% pure lactic acid.
Dairy products like cheese, yogurt, kefir and acidophilus milk are produced through fermentation.
Cheese is made through lactic acid fermentation of milk using starter cultures like Lactococcus lactis. Yogurt is produced using Streptococcus thermophilus and Lactobacillus delbrueckii subspecies bulgaricus. Kefir uses Lactococcus lactis, Lactobacillus delbrueckii subspecies bulgaricus and yeasts. Acidophilus milk production involves Lactobacillus acidophilus.
These fermented dairy products can deliver health benefits like aiding digestion, lowering cholesterol and potentially reducing cancer risks due to the probiotic
Sauerkraut is a fermented cabbage product with a sour taste made through the lactic acid fermentation of shredded cabbage by lactic acid bacteria such as Leuconostoc mesenteroides and Lactobacillus plantarum. The fermentation process typically takes 4-8 weeks to produce sauerkraut with an acidity level of around 1.7% and beneficial probiotic bacteria. Sauerkraut is high in fiber, vitamins, and minerals and consumption has various health benefits such as supporting digestive health and reducing inflammation.
1. The document discusses the production of lactic acid, glutamic acid, and cheese through fermentation processes. Lactic acid bacteria and fungi are used to produce lactic acid from sugars. Corynebacterium glutamicum is commonly used to produce glutamic acid from glucose through biosynthesis pathways.
2. The production of cheese involves pasteurizing milk, adding bacterial cultures, coagulating the milk with rennet enzyme, separating curd from whey, and ripening the curd through the action of molds and bacteria.
3. Specific microorganisms and fermentation steps are outlined for efficiently producing these three compounds at an industrial scale through microbial fermentation of sugars and carbohydrates.
Spirulina is a type of blue-green algae that is high in protein and nutrients. It can be consumed as a dietary supplement or used as an animal feed additive. Spirulina has 50-70% protein, more than beef or chicken, as well as fatty acids, vitamins, minerals, and phycocyanin pigment. It is commercially produced through cultivation in open ponds or closed photobioreactors. Proper temperature, lighting, water, and nutrients are needed for growth. The production process involves inoculation, cultivation, harvesting, dewatering, and drying the algal biomass. Operating parameters like light intensity, pH, and temperature must be carefully controlled.
Yogurt is made by fermenting milk with bacterial cultures such as Lactobacillus bulgaricus and Streptococcus thermophilus. The milk is pasteurized, inoculated with the cultures, held to ferment and thicken, and cooled before optional flavors or fruits are added. During fermentation, the cultures convert milk sugars into lactic acid, which coagulates the milk proteins to produce the yogurt's texture while the acidity prevents spoilage.
Single cell oils (SCOs) are oils extracted from microorganisms like algae, bacteria, and yeast. SCOs can serve as raw materials for oleochemical industries and be sources of nutrients. Researchers expressed an inulase gene in the yeast Yarrowia lipolytica to produce oil from inulin-containing materials. The recombinant yeast was able to accumulate 46.3% oil by weight when grown on inulin, converting around 10.7% of the sugar to cell oil which was composed primarily of fatty acids like palmitic acid, oleic acid, and stearic acid. Further research aims to improve oil yield by modifying additional genes in the lipid biosynthesis pathway.
introduction of Fermented food
Fermented foods are an extremely important part of human diet and worldwide may contribute to as much as one third of human diet.
Different types of fermented food isused in butter, cheese, bread, fermented vegetables,fermented meats etc.
The scope of food fermentation ranged from producing alcoholic beverages, fermented milk and vegetable products to genetically engineered super bugs to carry out efficient fermentation to treatment and utilization of waste and overall producing nutritious and safe products with appealing qualities.
2. Fermented Food Definition: Fermented foods are those food produced by modification of raw material of either animal or vegetable origin by the activities of microorganisms. Bacteria , yeast and moulds can be used to produce a diverse range of products that differ in flavor, texture and stability from the original raw material.
Or
Fermented foods are those foods which are subjected to action of microorganisms or enzymes to get desirable biochemical changes and cause significant modification to food.
Fermentation of vegetables and meat productsAman Kumar
This document discusses lactic acid fermentation of vegetables. It begins by explaining that vegetables naturally contain microflora that can be controlled through conditions like acidity or lack of nutrients. Lactic acid fermentation stabilizes this microflora. Starters are now used to initiate controlled fermentations. Many vegetables can undergo lactic acid fermentation including cabbage, carrots, cucumbers and olives. The process enhances quality and nutrition of the vegetables while restricting unwanted bacteria. Popular fermented vegetables from different regions like sauerkraut, kimchi and olives are then described in more detail.
The document discusses various fermented food products and the microbes involved in their production. It describes how bread and idli are produced through fermentation using microbes like Saccharomyces cerevisiae and Lactobacillus mesenteroides. It also discusses various cheeses like cheddar and their microbes such as Lactococcus lactis. Other fermented products mentioned include yogurt, kefir and acidophilus milk along with their associated health benefits and microbes.
This document discusses single cell oils (SCO), which are oils and fats produced through the microbial fermentation of sugar-rich media using oleaginous microorganisms like algae, yeasts and fungi. These microorganisms can accumulate up to 40% of their dry cell weight as lipids. The document outlines various microorganisms used for SCO production, cultivation methods like photobioreactors and open ponds, and extraction techniques for oils from microalgae including physical, chemical and enzymatic methods. Key advantages of SCO are the rapid growth and high yields of oleaginous microorganisms without requiring farmland.
This document discusses microbial flavors produced by microorganisms through metabolic processes. It describes several types of flavor compounds produced, including lactones, alcohols, aldehydes, and methyl ketones. Methods for microbial flavor production are outlined, such as de novo synthesis, biotransformation, and enzymatic methods using microbes like yeast, bacteria, and fungi. Examples of specific flavor compounds produced through these methods and the microbes involved are provided. Advantages of microbial flavor production are noted. The current status and market for microbial flavors is briefly summarized.
Microbial Polysaccharide - Food Application - Food IndustryMUTHUGANESAN N
Polysaccharides are the carbon sources which are found in huge amount in the biosphere
used for food, pharmaceutical, and medical applications
derives from the great diversity in structural and functional properties.
xanthan, xylinan, gellan, curdlan, pullulan, dextran, scleroglucan, schizophyllan, and cyanobacterial polysaccharides
The commercial value of polysaccharides is based on its ability to modify the flow characteristics of solutions (Rheology).
They can incr viscosity and hence used as thickening and gelling agents.
Microorganisms and their role in food technologyJOMIN JOSE
Microorganisms play an important role in food production and spoilage. Yeasts, molds and bacteria have been used for thousands of years to produce various fermented foods like bread, beer, wine, yogurt and cheese. Lactic acid bacteria are commonly used in fermented dairy products to provide flavor and inhibit undesirable microbes. Acetic acid bacteria oxidize sugars and alcohols during fermentation to produce organic acids. Yeasts like Saccharomyces cerevisiae are used in winemaking, baking and brewing due to their ability to grow in low pH environments and metabolize sugars. Molds can also be used to produce foods or food additives. However, some microbes like viruses can cause
This document discusses several types of fermented Asian foods including soy sauce, miso, sufu, natto, and idli. It describes the key ingredients and fermentation processes for each food. Soy sauce is produced from fermented soybeans, wheat, and saltwater using molds and bacteria. Miso is made from fermented soybeans with rice or barley and varies in taste depending on ingredients and fermentation time. Sufu involves drying and air fermenting tofu cubes with molds. Natto is made by fermenting soybeans with Bacillus subtilis. Idli involves the bacterial fermentation of rice and black gram dhal batter.
This document discusses various fermented milk products including cheese, yogurt, cultured buttermilk, acidophilus milk, and kefir. It provides details on the production processes and microorganisms involved in each product. Cheese is produced through fermentation of milk proteins and fats using bacteria and ripening. Yogurt is made by fermenting milk with Lactobacillus bulgaricus and Streptococcus thermophilus. Cultured buttermilk is the fluid remaining after sour cream or ripened cream is churned into butter. Acidophilus milk contains Lactobacillus acidophilus for potential health benefits. Kefir uses "kefir grains" containing various bacteria and yeasts to ferment milk
Beer is an alcoholic beverage made by fermenting grains like barley with hops and yeast. The key ingredients are barley, hops, yeast, and water. There are two main types - top fermented beer made with Saccharomyces cervisiae yeast and bottom fermented beer made with Saccharomyces carlsbergences yeast. Wine is an alcoholic beverage made by fermenting fruit juice, most commonly grape juice. The major steps in the production of beer and wine are preparation of ingredients, fermentation, aging or storage, and packaging. Microbial contamination and temperature fluctuations can affect the quality of beer and wine.
Single cell proteins (SCP) are dried cells of microorganisms that can be used as protein supplements for humans and animals. SCP production was first commercialized in the 1950s using bacteria cultured on methanol. Common microorganisms used for SCP production include fungi, yeast, algae and bacteria. Production involves selecting a suitable microorganism strain, fermenting it under controlled conditions, harvesting the cells, processing them, and isolating the protein. SCP have potential applications as nutritional supplements, health foods, and animal feed due to their protein and nutrient content.
Microbial production of oils and fats involves using microorganisms like yeast, fungi and algae to produce single cell oils (SCO) that contain fatty acids similar to those in plant and animal fats. SCO can be used as substitutes for plant oils in applications like animal feed, aquaculture feed, and biodiesel. Key steps in SCO production include cultivating the microorganisms in conditions of excess carbon and limited nitrogen to trigger lipid accumulation, then extracting the oils. Important SCOs include arachidonic acid and docosahexaenoic acid which have health benefits and are used in infant formula. Though SCO production has higher costs than plant oils, it provides benefits like independence from climate and geography.
The document discusses important industrial microorganisms used in biotechnology and their applications. It describes how industrial microbes like bacteria, fungi, yeast, algae and viruses are employed in mass production of chemicals, foods, fuels, enzymes and antibiotics. Specific examples mentioned include using lactobacillus bacteria in yogurt production, streptomyces bacteria for antibiotics like erythromycin, penicillium fungi for penicillin, and yeast for ethanol fermentation. The document outlines properties of useful industrial microbes and how they are categorized based on their metabolic products and the industries they impact.
This document discusses solid state fermentation and provides details about the process. It describes that solid state fermentation involves fermentation using solids in the absence of free water, though some moisture is needed. Microorganisms like fungi grow on the surface of solid substrates to produce things like enzymes, organic acids, and flavors. Agriculture wastes are commonly used as substrates. Fungi like Trichoderma and Aspergillus species are widely used to produce hydrolytic enzymes. Tray fermenters and rotating drum reactors are two common types of bioreactors used in solid state fermentation.
Cheese production involves several key steps:
1) Curdling of milk through the addition of starter cultures or rennet, which causes casein to coagulate and separate from whey.
2) Draining the curd to remove moisture and separate whey.
3) Salting the curd, which acts as a preservative and controls moisture.
4) Ripening the curd through bacterial or mold cultures, during which flavor and texture develop.
Different cheeses are produced by varying the cultures, temperatures, and other conditions during the production process.
This document discusses single cell protein (SCP), which refers to protein extracted from microorganisms like yeast, algae, fungi and bacteria that can be used as a protein supplement for humans and animals. It provides details on the history of SCP, production methods using various microorganisms, advantages like rapid growth and high protein content, disadvantages like possible toxins and poor digestibility, and applications as a food supplement, in health products, cosmetics, and animal feed. Spirulina is highlighted as a commonly used algae for SCP production due to its high protein content and ease of harvesting. The document concludes that SCP production is still developing but has potential as a sustainable food source.
This document discusses single cell proteins (SCP), which are dried cells of microorganisms that can be used as a dietary protein supplement. SCPs are produced using biomass as a raw material and various microorganisms like fungi, algae, and bacteria that are cultured on the biomass. The production involves selecting suitable microorganism strains, fermenting them, harvesting the cells, and processing them for use as a protein supplement in foods. SCPs have advantages like being a renewable source of protein but also have disadvantages like potentially high nucleic acid content.
Spirulina is a type of blue-green algae that is high in protein and nutrients. It can be consumed as a dietary supplement or used as an animal feed additive. Spirulina has 50-70% protein, more than beef or chicken, as well as fatty acids, vitamins, minerals, and phycocyanin pigment. It is commercially produced through cultivation in open ponds or closed photobioreactors. Proper temperature, lighting, water, and nutrients are needed for growth. The production process involves inoculation, cultivation, harvesting, dewatering, and drying the algal biomass. Operating parameters like light intensity, pH, and temperature must be carefully controlled.
Yogurt is made by fermenting milk with bacterial cultures such as Lactobacillus bulgaricus and Streptococcus thermophilus. The milk is pasteurized, inoculated with the cultures, held to ferment and thicken, and cooled before optional flavors or fruits are added. During fermentation, the cultures convert milk sugars into lactic acid, which coagulates the milk proteins to produce the yogurt's texture while the acidity prevents spoilage.
Single cell oils (SCOs) are oils extracted from microorganisms like algae, bacteria, and yeast. SCOs can serve as raw materials for oleochemical industries and be sources of nutrients. Researchers expressed an inulase gene in the yeast Yarrowia lipolytica to produce oil from inulin-containing materials. The recombinant yeast was able to accumulate 46.3% oil by weight when grown on inulin, converting around 10.7% of the sugar to cell oil which was composed primarily of fatty acids like palmitic acid, oleic acid, and stearic acid. Further research aims to improve oil yield by modifying additional genes in the lipid biosynthesis pathway.
introduction of Fermented food
Fermented foods are an extremely important part of human diet and worldwide may contribute to as much as one third of human diet.
Different types of fermented food isused in butter, cheese, bread, fermented vegetables,fermented meats etc.
The scope of food fermentation ranged from producing alcoholic beverages, fermented milk and vegetable products to genetically engineered super bugs to carry out efficient fermentation to treatment and utilization of waste and overall producing nutritious and safe products with appealing qualities.
2. Fermented Food Definition: Fermented foods are those food produced by modification of raw material of either animal or vegetable origin by the activities of microorganisms. Bacteria , yeast and moulds can be used to produce a diverse range of products that differ in flavor, texture and stability from the original raw material.
Or
Fermented foods are those foods which are subjected to action of microorganisms or enzymes to get desirable biochemical changes and cause significant modification to food.
Fermentation of vegetables and meat productsAman Kumar
This document discusses lactic acid fermentation of vegetables. It begins by explaining that vegetables naturally contain microflora that can be controlled through conditions like acidity or lack of nutrients. Lactic acid fermentation stabilizes this microflora. Starters are now used to initiate controlled fermentations. Many vegetables can undergo lactic acid fermentation including cabbage, carrots, cucumbers and olives. The process enhances quality and nutrition of the vegetables while restricting unwanted bacteria. Popular fermented vegetables from different regions like sauerkraut, kimchi and olives are then described in more detail.
The document discusses various fermented food products and the microbes involved in their production. It describes how bread and idli are produced through fermentation using microbes like Saccharomyces cerevisiae and Lactobacillus mesenteroides. It also discusses various cheeses like cheddar and their microbes such as Lactococcus lactis. Other fermented products mentioned include yogurt, kefir and acidophilus milk along with their associated health benefits and microbes.
This document discusses single cell oils (SCO), which are oils and fats produced through the microbial fermentation of sugar-rich media using oleaginous microorganisms like algae, yeasts and fungi. These microorganisms can accumulate up to 40% of their dry cell weight as lipids. The document outlines various microorganisms used for SCO production, cultivation methods like photobioreactors and open ponds, and extraction techniques for oils from microalgae including physical, chemical and enzymatic methods. Key advantages of SCO are the rapid growth and high yields of oleaginous microorganisms without requiring farmland.
This document discusses microbial flavors produced by microorganisms through metabolic processes. It describes several types of flavor compounds produced, including lactones, alcohols, aldehydes, and methyl ketones. Methods for microbial flavor production are outlined, such as de novo synthesis, biotransformation, and enzymatic methods using microbes like yeast, bacteria, and fungi. Examples of specific flavor compounds produced through these methods and the microbes involved are provided. Advantages of microbial flavor production are noted. The current status and market for microbial flavors is briefly summarized.
Microbial Polysaccharide - Food Application - Food IndustryMUTHUGANESAN N
Polysaccharides are the carbon sources which are found in huge amount in the biosphere
used for food, pharmaceutical, and medical applications
derives from the great diversity in structural and functional properties.
xanthan, xylinan, gellan, curdlan, pullulan, dextran, scleroglucan, schizophyllan, and cyanobacterial polysaccharides
The commercial value of polysaccharides is based on its ability to modify the flow characteristics of solutions (Rheology).
They can incr viscosity and hence used as thickening and gelling agents.
Microorganisms and their role in food technologyJOMIN JOSE
Microorganisms play an important role in food production and spoilage. Yeasts, molds and bacteria have been used for thousands of years to produce various fermented foods like bread, beer, wine, yogurt and cheese. Lactic acid bacteria are commonly used in fermented dairy products to provide flavor and inhibit undesirable microbes. Acetic acid bacteria oxidize sugars and alcohols during fermentation to produce organic acids. Yeasts like Saccharomyces cerevisiae are used in winemaking, baking and brewing due to their ability to grow in low pH environments and metabolize sugars. Molds can also be used to produce foods or food additives. However, some microbes like viruses can cause
This document discusses several types of fermented Asian foods including soy sauce, miso, sufu, natto, and idli. It describes the key ingredients and fermentation processes for each food. Soy sauce is produced from fermented soybeans, wheat, and saltwater using molds and bacteria. Miso is made from fermented soybeans with rice or barley and varies in taste depending on ingredients and fermentation time. Sufu involves drying and air fermenting tofu cubes with molds. Natto is made by fermenting soybeans with Bacillus subtilis. Idli involves the bacterial fermentation of rice and black gram dhal batter.
This document discusses various fermented milk products including cheese, yogurt, cultured buttermilk, acidophilus milk, and kefir. It provides details on the production processes and microorganisms involved in each product. Cheese is produced through fermentation of milk proteins and fats using bacteria and ripening. Yogurt is made by fermenting milk with Lactobacillus bulgaricus and Streptococcus thermophilus. Cultured buttermilk is the fluid remaining after sour cream or ripened cream is churned into butter. Acidophilus milk contains Lactobacillus acidophilus for potential health benefits. Kefir uses "kefir grains" containing various bacteria and yeasts to ferment milk
Beer is an alcoholic beverage made by fermenting grains like barley with hops and yeast. The key ingredients are barley, hops, yeast, and water. There are two main types - top fermented beer made with Saccharomyces cervisiae yeast and bottom fermented beer made with Saccharomyces carlsbergences yeast. Wine is an alcoholic beverage made by fermenting fruit juice, most commonly grape juice. The major steps in the production of beer and wine are preparation of ingredients, fermentation, aging or storage, and packaging. Microbial contamination and temperature fluctuations can affect the quality of beer and wine.
Single cell proteins (SCP) are dried cells of microorganisms that can be used as protein supplements for humans and animals. SCP production was first commercialized in the 1950s using bacteria cultured on methanol. Common microorganisms used for SCP production include fungi, yeast, algae and bacteria. Production involves selecting a suitable microorganism strain, fermenting it under controlled conditions, harvesting the cells, processing them, and isolating the protein. SCP have potential applications as nutritional supplements, health foods, and animal feed due to their protein and nutrient content.
Microbial production of oils and fats involves using microorganisms like yeast, fungi and algae to produce single cell oils (SCO) that contain fatty acids similar to those in plant and animal fats. SCO can be used as substitutes for plant oils in applications like animal feed, aquaculture feed, and biodiesel. Key steps in SCO production include cultivating the microorganisms in conditions of excess carbon and limited nitrogen to trigger lipid accumulation, then extracting the oils. Important SCOs include arachidonic acid and docosahexaenoic acid which have health benefits and are used in infant formula. Though SCO production has higher costs than plant oils, it provides benefits like independence from climate and geography.
The document discusses important industrial microorganisms used in biotechnology and their applications. It describes how industrial microbes like bacteria, fungi, yeast, algae and viruses are employed in mass production of chemicals, foods, fuels, enzymes and antibiotics. Specific examples mentioned include using lactobacillus bacteria in yogurt production, streptomyces bacteria for antibiotics like erythromycin, penicillium fungi for penicillin, and yeast for ethanol fermentation. The document outlines properties of useful industrial microbes and how they are categorized based on their metabolic products and the industries they impact.
This document discusses solid state fermentation and provides details about the process. It describes that solid state fermentation involves fermentation using solids in the absence of free water, though some moisture is needed. Microorganisms like fungi grow on the surface of solid substrates to produce things like enzymes, organic acids, and flavors. Agriculture wastes are commonly used as substrates. Fungi like Trichoderma and Aspergillus species are widely used to produce hydrolytic enzymes. Tray fermenters and rotating drum reactors are two common types of bioreactors used in solid state fermentation.
Cheese production involves several key steps:
1) Curdling of milk through the addition of starter cultures or rennet, which causes casein to coagulate and separate from whey.
2) Draining the curd to remove moisture and separate whey.
3) Salting the curd, which acts as a preservative and controls moisture.
4) Ripening the curd through bacterial or mold cultures, during which flavor and texture develop.
Different cheeses are produced by varying the cultures, temperatures, and other conditions during the production process.
This document discusses single cell protein (SCP), which refers to protein extracted from microorganisms like yeast, algae, fungi and bacteria that can be used as a protein supplement for humans and animals. It provides details on the history of SCP, production methods using various microorganisms, advantages like rapid growth and high protein content, disadvantages like possible toxins and poor digestibility, and applications as a food supplement, in health products, cosmetics, and animal feed. Spirulina is highlighted as a commonly used algae for SCP production due to its high protein content and ease of harvesting. The document concludes that SCP production is still developing but has potential as a sustainable food source.
This document discusses single cell proteins (SCP), which are dried cells of microorganisms that can be used as a dietary protein supplement. SCPs are produced using biomass as a raw material and various microorganisms like fungi, algae, and bacteria that are cultured on the biomass. The production involves selecting suitable microorganism strains, fermenting them, harvesting the cells, and processing them for use as a protein supplement in foods. SCPs have advantages like being a renewable source of protein but also have disadvantages like potentially high nucleic acid content.
This document discusses various fermentation techniques used in industrial bioprocesses. It begins by defining fermentation and describing fermentation techniques. There are several types of fermentations described - batch, continuous, fed-batch, anaerobic, aerobic, surface, submerged, and solid-state fermentations. Each type is briefly explained highlighting its key characteristics and industrial applications. Important fermentation products like ethanol, glycerol, lactic acid are also listed. The document concludes by stating that traditional fermentations will remain important in food production and future research should identify risks and benefits of specific indigenous fermented products.
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CONTENTS-
Introduction
• History
• SCP production in India
• Raw materials
• SCP production
• Advantages and Disadvantages
• Applications
• Conclusion
• References
Fermentation is a process where microorganisms such as bacteria and yeasts produce enzymes and other products. There are two main types of fermentation used to produce enzymes - submerged fermentation and solid-state fermentation. Submerged fermentation involves growing microorganisms in a liquid nutrient media while solid-state fermentation uses a solid substrate. Enzymes can be recovered after fermentation through methods like centrifugation. Fermentation is used across many industries like brewing, baking, and cheese making to produce important products.
Single cell protein (SCP) can be produced from microorganisms like algae, fungi, yeast and bacteria. SCP has the potential to relieve protein deficiency by being used directly as a human food supplement or indirectly by partially replacing soybean meal and fish proteins in animal feed. Microorganisms used for SCP production must meet certain criteria like being non-pathogenic, nutritious, easily produced at large scale, toxin-free, and fast-growing. Different microorganisms have different advantages and disadvantages for SCP production. Nucleic acid levels must be reduced for human consumption. SCP is produced through fermentation using various carbon sources and nutrients, followed by biomass recovery, processing, and evaluation to
Fermentation is a process that uses microorganisms to produce food, pharmaceuticals, and alcoholic beverages on an industrial scale. There are three main types of fermentation processes: batch, fed-batch, and continuous. Batch fermentation involves adding all nutrients at once and allowing the microbes to grow until maximum product concentration is reached. Fed-batch fermentation involves regularly adding fresh media without removing culture. Continuous fermentation continuously removes and replenishes culture media and products to maintain steady conditions. Fermenters come in various sizes and designs including surface, submerged, stirred tank, airlift, and bubble column fermenters. Proper media formulation providing carbon, nitrogen, minerals and other nutrients is essential for microbial growth
Single cell proteins are dried cells from microorganisms like yeast, algae, fungi and bacteria that are used as a protein supplement for animal feed and potentially human consumption. They are produced by fermenting the microorganisms in large vessels using various biomass sources as a carbon source for rapid growth. The harvested cells are processed to extract and purify the protein content while removing other cell components like nucleic acids. Single cell proteins offer benefits like a high protein content, ability to use low-cost substrates, and potential to address malnutrition if produced safely for human use.
Fermentation is defined as the conversion of carbohydrates like sugars and starches into alcohol and acids through the metabolic process of microorganisms like yeast and bacteria under anaerobic conditions. There are several types of fermentation including solid state fermentation, submerged fermentation, anaerobic fermentation, and aerobic fermentation. Key factors that affect fermentation include temperature, pH, oxygen levels, and nutrients available to the microorganisms. Common examples of fermentation products include yogurt, wine, beer, and bread.
This document discusses the production of vitamin B12 and riboflavin through microbial fermentation. It outlines the key microorganisms used, including Streptomyces griseus and Streptomyces olivaceus for vitamin B12 production, and Ashbya gossypii for riboflavin production. The document also provides an overview of the fermentation process, noting that it is carried out through submerged fermentation over 3-5 days, requiring aeration and agitation of the growth medium. Details are given on inoculum preparation and scaling up for vitamin B12 production using S. olivaceus.
Single cell proteins are dried cells of microorganisms that can be used as a dietary protein supplement for humans and animals. They are produced by growing microorganisms like yeast, algae, fungi and bacteria on various substrates. The microorganisms are harvested and processed to extract protein. Single cell proteins have high protein content and offer a sustainable source of nutrition but their production requires sterile conditions and the protein extract may cause allergic reactions in some people.
Fermentation is a process by which microorganisms break down nutrients and produce useful products. There are several types of fermentation processes including solid state, submerged, anaerobic, and aerobic fermentation. Key requirements for fermentation include microorganisms, nutrients for growth of the microorganisms, and control of environmental factors like temperature, pH, and oxygen levels. Common fermentation products include antibiotics, organic acids, vitamins, and enzymes. Fermentors provide the controlled environment needed for efficient fermentation.
1) The document discusses various microbial culture techniques used in food processing and research laboratories. It describes batch, continuous, fed-batch, and synchronous culture techniques.
2) It also categorizes the microbes used in food processing as bacteria, yeasts, and moulds. It provides examples of key bacteria like Lactobacillus species used in food manufacturing as starter cultures.
3) The document concludes that fermented foods produced using microbial cultures contribute significantly to human nutrition and health by providing preservation, enrichment and health benefits.
ANTIBIOTICS /BETA-LACTUM PHARMACOGNOSY PRODUCTION IN DETAILS .pptxsriramsawarni
Pharmacognosy antibiotics sources and productions, beta lactum antibiotics and productions and cephalosporin and monobactum antibiotics production
B.pharm 6th semester B.pharm 6th semester ARYABHATT KNOWELDGE UNIVERSITY PATNA BIHAR
AKU PATNA
A broad module on industrial microbiology is summarized with pictures .It includes the production of vitamins,vaccine ,alcohol,vinegar,steroids,amino acids ,antibiotics .it also includes the general idea on history ,media,equipment,fermentation,procedure ,uses of industrial microbiology .The production of wine,beer and vinegar are mine core interest .Hope may help ....Thank you .
The process of growing microorganisms in culture by taking bacteria from the infection site (in vivo or environment) and grow them in artificial environment in the laboratory (in vitro).
Bacteria may require adequate nutrition, optimum pH, temperature and oxygen for growth and multiplication.
Suitable artificial media containing sources of carbon, nitrogen, hydrogen, oxygen, phosphorous and other elements such as sodium, potassium, magnesium, iron and growth factor (Vitamins) in very small amounts have been used for cultivation of microorganism.
When microorganisms are cultivated in the laboratory, a growth environment called a medium is used. The medium may be purely chemical (a chemically defined medium), or it may contain organic materials, or it may consist of living organisms such as fertilized eggs.
Microorganisms growing in or on such a medium form a culture.
A culture is considered a pure culture if only one type of organism is present and a mixed culture if populations of different organisms are present.
When first used, the culture medium should be sterile, meaning that no form of life is present before inoculation with the microorganism.
Single cell protein (SCP) refers to edible microorganisms or their extracts used as a protein supplement. SCP can be produced using bacteria, yeast, fungi or algae through fermentation. It has high nutritional value but also has some limitations. Research is focused on improving production methods and addressing issues like high nucleic acid content and digestibility. SCP shows potential as a sustainable protein source but more work is needed before it will be widely accepted as human food.
This document discusses industrial fermentation processes. It begins by defining fermentation as biochemical processes carried out by microorganisms. Fermentation is used to produce compounds in industries like energy, materials, pharmaceuticals, chemicals and food. Microbes are grown under controlled conditions to produce useful end products. Common industrial fermentation products include wine, beer, vinegar, ethanol, antibiotics and enzymes. The document then discusses the basic principles and methodology of industrial fermentation, including typical fermentation equipment, growth phases of microbes, and the general fermentation procedure. It provides examples of specific industrial fermentation processes like penicillin production, streptomycin production and riboflavin production.
Bacterial enzymes and industrial enzymes are important for many industries. Bacterial enzymes like amylase, protease, and cellulase are produced through fermentation of bacteria like Bacillus subtilis. The production process involves selecting a microorganism, isolating it in pure culture, improving the strain, formulating growth media, fermentation, and recovering the enzymes. Industrial enzymes have various applications in industries like textiles, detergents, food, and pulp/paper. Examples are amylases for desizing fabrics and dish detergents, proteases for removing stains, and cellulases for biopolishing textiles.
ESA/ACT Science Coffee: Diego Blas - Gravitational wave detection with orbita...Advanced-Concepts-Team
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Speaker: Diego Blas (IFAE/ICREA)
Title: Gravitational wave detection with orbital motion of Moon and artificial
Abstract:
In this talk I will describe some recent ideas to find gravitational waves from supermassive black holes or of primordial origin by studying their secular effect on the orbital motion of the Moon or satellites that are laser ranged.
ESR spectroscopy in liquid food and beverages.pptxPRIYANKA PATEL
With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
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Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
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photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
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A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
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MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
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measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
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spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
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The technology uses reclaimed CO₂ as the dyeing medium in a closed loop process. When pressurized, CO₂ becomes supercritical (SC-CO₂). In this state CO₂ has a very high solvent power, allowing the dye to dissolve easily.
2. SOURCES OF PROTEIN :
https://i.pinimg.com/originals/1e/b4/d
8/1eb4d8c50999fc2cf19cc018ef2c3d4d.
jpg
3. LIMITATIONS:
• crop failure due to unfavorable climatic conditions.
• need a time lapse for the replenishment of stock .
• limited land available for farming .
4. SCP?
• Single cell protein (SCP)
• refers to the dried form of a group of microorganisms’ biomasses.
• Term coined by Professor Carroll L. Wilson.
• Also known as ‘Novel food’ & ‘Mini food’.
• having algal, bacterial or fungal origin.
• protein source for human food supplements and animal feeds.
5. WHY TO USE MICROORGANISMS FOR THE
PRODUCTION OF SCP :
• High quantity of protein produced.
• quality of protein generated is superior.
• easy to manipulate genetically - diverse amino acid compositions.
• Waste products can be employed as a substrate.
• The fermentation procedures, as well as the culture conditions, are both simple.
• The generation of microorganisms is not affected by environmental factors.
6. HISTORY :
Developed during war time
World War I - Saccharomyces
cerevisiae – Germany- from
molasses to replace up to 60% of
imported protein.
World War II -Candida utilis on
sulphite liquor from paper
manufacturing wastes.
7. REASONS :
• conventional foods were in short supply.
• recognized that protein malnutrition is usually far more severe.
• microorganisms would help meet this world protein deficiency.
8. AFTER EFFECTS :
• several plants were built in the US and Europe, mainly for C. utilis production.
• In 1950’s British Petroleum initiated production of SCP on commercial basis.
9. PRUTEEN – (1970s)
• was the 1st commercial SCP
• used as animal feed additive
• had 72 % protein content.
11. IN RECENT TIMES :
• among the European communist countries the USSR had the largest capacity for
SCP production with at least 86 plants in operation using different substrates.
• To date, a profusion of reports about SCP production has appeared in the
scientific literature.
12. TWO MAIN APPROACHES HAVE BEEN FOLLOWED:
• utilization of conventional substrates.
• use of waste materials where SCP production brings about pollution control.
13. SCP PRODUCTION IN INDIA
• CSIR- National Botanical Research Institute (NBRI) Lucknow U.P.
• Central Food Technological Research Institute (CFTRI) Mysore Karnataka.
• Vigyan Institute of Pharmaceutical Technology, Vishakhapatnam A.P.
14. ORGANISMS TO BE USED IN SCP PRODUCTION
SHOULD HAVE THE FOLLOWING PROPERTIES:
1. Absence of pathogenicity and toxicity.
2. Amount of protein should be high & contain amino acids required by man.
3. should be digestible, possess acceptable taste and aroma.
4. must grow rapidly in a cheap, easily available medium.
5. Adaptability to unusual environmental conditions
15. PROBLEMS RELATED WITH SCP :
• many developing countries, lack the expertise and/or the financial resources to develop
the highly capital intensive fermentation industries involved.
Short-coming bridged by the use of improvised fermenters and recovery methods which
do not require sophisticated equipment.
16. ANOTHER PROBLEM:
• Urate oxidase / Uricase
• Absent in higher primates including human.
oxidizes uric acid to the soluble
and excretable allantoin.
17. PROCESSING OF RNA BY BODY :
nucleases - pancreatic juice
+ intestinal juices
converted into nucleosides
nucleic acid eaten
URIC ACID
ALLANTONIN
18. WHY REMOVAL OF RNA ?
• As a result when foods rich in nucleic acid are consumed in large amounts, an
unusually high level of uric acid occurs in the blood plasma.
• Owing to the low solubility of uric acid, uricates may be deposited in various
tissues in the body including the kidneys and the joints leading to kidney stone
formation and gout.
19. WAYS FOR REMOVAL OF NUCLEIC ACID FROM SCP:
Growth and cell physiology method :
• higher the growth rate higher the RNA content.
• The growth rate is therefore reduced as a means of reducing nucleic acid.
• high growth is one of the requirements of reducing costs in SCP.
• hence the method may have only limited usefulness.
20. CONTINUED…
Extraction with chemicals :
• Dilute bases such as NaOH or KOH will hydrolyze RNA easily.
• Hot 10% sodium chloride may also be used to extract RNA.
• The cells usually have to be disrupted before using these methods. In some cases
the protein may then be extracted, purified and concentrated.
21. CONTINUED….
Use of pancreatic juice:
• RNAase from bovine pancreatic juice, which is heat-stable, has been used to
hydrolyze yeast RNA at 80°C at which the cells are more permeable.
Activation of endogenous RNA:
• The RNAase of the organism itself may be activated by heat-shock or by
chemicals.
• The RNA content of yeasts have been reduced in this way.
24. PRINCIPLE USED :
most commonly used principle -chemostat:
a perfectly mixed suspension of biomass into which medium is fed at a constant
rate and the culture is harvested at the same rate so that the culture volume
remains constant.
26. BATCH FERMENTATION :
limited amount of
sterilized nutrient
medium
inoculated with a
suitable
microorganism &run
for a definite period
or until the nutrients
are exhausted
culture broth is
harvested and the
product is
separated
Oxygen in the form of air, an antifoam agent and acid
or base, to control the pH,
27. CONTINUOUS FERMENTATION :
• As a result, volume of the medium and concentration of nutrients at optimum level are being
maintained
Closed fermenter
containing medium and
inoculum forming
cultured medium
fresh nutrient medium
is added
continuously/
intermittently
equivalent amount of
used medium with
microorganisms is
withdrawn
continuously/
intermittently
28. FED BATCH FERMENTATION:
• modification to the batch fermentation.
• substrate is added periodically in installments
• as the fermentation progresses, due to which the substratum is always at an optimal
concentration.
• This is essential as some secondary metabolites are subjected to catabolite repression by high
concentration of either glucose, or other carbohydrate or nitrogen compounds present in the
medium.
• For this reason, the critical elements of the nutrient medium are added in low amount in the
beginning of the fermentation and these substrates continue to be added in small doses during
the production phase.
• generally employed for the production of penicillin.
• Yoshida (1973) introduced this term for the first time for feeding the substrates to the medium
as the nutrients are exhausted, so as to maintain the nutrients at an optimum level.
29. ANAEROBIC FERMENTATION :
• fermentation carried out in the absence of oxygen.
• two types : obligate anaerobic microorganisms e.g., Clostridium sp. and facultative
anaerobic microorganisms e.g., lactic acid bacteria.
• Anaerobic conditions are created either by withdrawing the oxygen present in the
head space by an exhaust pump and pumping some inert gases like nitrogen, argon
etc. or by flushing it out, by the emergence of certain gases like carbon dioxide or
hydrogen
• Stationary medium and viscous medium also creates anaerobic conditions.
• Sometimes in order to create anaerobic condition, medium is inoculated at the
bottom of the fermenter soon after sterilization.
30. AEROBIC FERMENTATION :
• fermentation carried out in the presence of oxygen.
• In most of the commercial processes and majority of the products of human
utility are produced by this type of fermentation.
• Fermentation can be surface culture or static and submerged.
31. SURFACE FERMENTATIONS:
• substratum may be solid or liquid.
• The organism grows and draws the nutrients from the substratum.
• These types of fermentations are desirable where the products are based on sporulation.
• But it has several disadvantages such as it exposes the organism to unequal conditions,
both oxygen and nutrients.
32. SUBMERGED FERMENTATIONS
• nutrient substratum is liquid and the organism grows inside the substratum.
• The culture conditions are made uniform with the help of spargers and impeller
blades.
• Liquid state substratum is also called as broth.
33. SOLID SUBSTRATE/STATE FERMENTATION:
• growth of the microorganism on moist solid materials in the absence or near the
absence of free water.
• substrate itself acts as carbon source.
• employs a natural substrate as above or an inert substrate used as solid support.
• normally many step process involving.
34. HARVESTING :
• The biomass from yeast fermentation processes is harvested normally by continuous
centrifugation.
• Filamentous fungi are harvested by filtration.
• biomass is then treated for RNA reduction and dried in steam drums of spray driers.
Drying is expensive, but results in stabilized product with shelf lives of years.
• Generally, under combined conditions of low water activity and presence of
intractable solid substrate, fungi show luxuriant growth.
• Hence, proper growth of fungi in Solid state fermentation gives much higher
concentration of the biomass and higher yield when compared to submerged
fermentation
36. ALGAE AS SCP :
• microscopic single-cell true algae or prokaryotic cyanobacteria, grown with the
use of carbon dioxide and light energy (autotrophic growth).
• rich in vitamins, especially water-soluble vitamins, and essential fatty acids.
• amino acid content is balanced except, Sulphur-containing amino acids
methionine and cystine.
• cell wall is not readily digestible, treatment to disrupt the cell wall structure will
increase digestibility and hence nutritional value.
37. USES :
• cultivation of daphnid that thrive on plankton as a food source in aquaculture.
• feed for chicken and swine
• Chlorella & Spirulina for the purpose of food.
• preparation of tablets
• protein and vitamin supplements, or to help people lose weight.
• sold as dry powder or as pellets.
• additive or supplement to cereal foodstuffs
• as a garnish to salads.
• mixtures with doughs for baked goods and pasta, such as bread, rolls, cookies and noodles.
• In Mexico, S. maxima has been used as a supplement for biscuits produced by a state company as part of a national breakfast
programme for schoolchildren.
38. DISADVANTAGES :
• bitter flavor
• presence of dark green pigments which are difficult to mask.
• In addition to chlorophylls, other pigments such as carotenes, xanthines and
phycocyanin are present in varying amounts.
Flavor and color may be improved if algal biomass is treated during downstream
processing to remove undesirable components.
39. FUNGI AS SCP :
• fungal protein product, called Quorn,
• meat substitute manufactured by a single strain of a filamentous saprotrophic
ascomycete, Fusarium venenatum.
• produced from a multi-cellular, filamentous fungus, mycoprotein is the preferred
name.
• consumption could raise uric acid levels in the blood and lead to gout and other
illnesses.
• addressed by heating the mycelium at 68 °C for 20 min, which allows endogenous
enzymes to destroy much of the RNA without reducing its protein content.
• then dried and bound with egg white, creates the meaty texture.
40. APPLICATIONS :
• Obesity, stress, weight, cholesterol, and blood sugar levels can all be controlled
with this therapeutic and pharmaceutical use.
• Application for hair and skin care products.
• Malnourished people can benefit from a protein supplement.
• A convenient feeding source for poultry, fisheries, and animal farms.
41. REFERENCES :
• FOOD & INDUSTRIAL MICROBIOLOGY by R.K. MALIK, SUJA SENAN & SHILPA VIJ
• URICASE ENZYME PROTEIN RECOMBINANT BY PROSEC.
• https://www.sciencedirect.com/science/article/pii/B0122270703014501
• Single cell protein yeasts & bacteria by Mariano García Garibay, Eduardo Bárzana, in Encyclopedia of
Food Microbiology, 1999
• M. García-Garibay, E. Bárzana, in Encyclopedia of Food Microbiology (Second Edition), 2014
• https://www.sciencedirect.com/science/article/pii/B9780123820341000128
• https://www.sciencedirect.com/science/article/pii/B9780128012246000072
• https://www.biotechnologynotes.com/industrial-biotechnology/fermentation-process/fermentation-
types-8-types-of-fermentations-industrial-biotechnology/13695