Fruit Loops
  DNA Extraction
Breaking Down Cell Structure
       to Extract DNA



     © T. Rosa & B.R. Shmaefsky   1
Cell Structure
                                             Cell Function
                                             Review

   Ribosome

                                              Endoplasmic
Chromosomes
                                              Reticulum


 Nucleus                                            Lysosome
                                                    (Vacuole)
Nuclear
Membrane
                                                     Golgi
                                                     Body

                                                 Cell Membrane
Cytoplasm
                                           Mitochondria
              © T. Rosa & B.R. Shmaefsky     2
Name that function…
   Ribosome

                                                Endoplasmic
Chromosomes
                                                Reticulum


 Nucleus                                              Lysosome
                                                      (Vacuole)
Nuclear
Membrane
                                                       Golgi
                                                       Body

                                                   Cell Membrane
Cytoplasm
                                             Mitochondria
                © T. Rosa & B.R. Shmaefsky     3
Making our way to the DNA




       © T. Rosa & B.R. Shmaefsky   4
Step 1
     Breaking down the cell
          membrane
How do you break down fats?




                        Cell membrane is a layer of lipid (fat)

                © T. Rosa & B.R. Shmaefsky       5
Step 2
Mix, mix, and more mixing
                        Mixing the cells in the soap
                          breaks down the cell
    La, la, la…
                          membrane and the
                          nuclear membrane to get
                          us to the DNA.




          © T. Rosa & B.R. Shmaefsky      6
Step 3
Filtering out the chunks
                           We filter out the parts of
                              the cell that we do not
                              need:
                           1. Cell membranes
                           2. Nuclear membranes
                           3. Cell organelles
   I’m in here!




          © T. Rosa & B.R. Shmaefsky       7
Step 4
Alcohol Separation
                     Alcohol denatures the DNA
                       and separates it from the
                       liquid by making it
                       clump.

    Denaturing changes
    the structure of the
    molecule


      Denatured DNA

   © T. Rosa & B.R. Shmaefsky       8
Test your knowledge
• Why is it so difficult to get to the DNA?
• What process removes the DNA from the
  cell?
• Why would scientists need to extract
  DNA?
• Can you think of other substances that we
  can extract DNA from?
              © T. Rosa & B.R. Shmaefsky   9

Simple DNA Extraction Background

  • 1.
    Fruit Loops DNA Extraction Breaking Down Cell Structure to Extract DNA © T. Rosa & B.R. Shmaefsky 1
  • 2.
    Cell Structure Cell Function Review Ribosome Endoplasmic Chromosomes Reticulum Nucleus Lysosome (Vacuole) Nuclear Membrane Golgi Body Cell Membrane Cytoplasm Mitochondria © T. Rosa & B.R. Shmaefsky 2
  • 3.
    Name that function… Ribosome Endoplasmic Chromosomes Reticulum Nucleus Lysosome (Vacuole) Nuclear Membrane Golgi Body Cell Membrane Cytoplasm Mitochondria © T. Rosa & B.R. Shmaefsky 3
  • 4.
    Making our wayto the DNA © T. Rosa & B.R. Shmaefsky 4
  • 5.
    Step 1 Breaking down the cell membrane How do you break down fats? Cell membrane is a layer of lipid (fat) © T. Rosa & B.R. Shmaefsky 5
  • 6.
    Step 2 Mix, mix,and more mixing Mixing the cells in the soap breaks down the cell La, la, la… membrane and the nuclear membrane to get us to the DNA. © T. Rosa & B.R. Shmaefsky 6
  • 7.
    Step 3 Filtering outthe chunks We filter out the parts of the cell that we do not need: 1. Cell membranes 2. Nuclear membranes 3. Cell organelles I’m in here! © T. Rosa & B.R. Shmaefsky 7
  • 8.
    Step 4 Alcohol Separation Alcohol denatures the DNA and separates it from the liquid by making it clump. Denaturing changes the structure of the molecule Denatured DNA © T. Rosa & B.R. Shmaefsky 8
  • 9.
    Test your knowledge •Why is it so difficult to get to the DNA? • What process removes the DNA from the cell? • Why would scientists need to extract DNA? • Can you think of other substances that we can extract DNA from? © T. Rosa & B.R. Shmaefsky 9