An existing intravenous infusion technique for administering pharmaceuticals to nonhuman primates was transferred to a new laboratory setting. As part of the technique transfer, port catheters were surgically implanted in two cynomolgus monkeys (Macaca fasicularis) and the animals underwent a 4-week recovery. Continuous intravenous infusion of an unspecified substance was then administered for 24 hours using a portable infusion pump in a backpack. The technique transfer was successful, with no significant clinical issues observed during recovery or dosing. Post-mortem examination found common histopathological lesions at the catheter tip and port site. The transferred technique allows longer intravenous dosing studies in cynomolgus monkeys in accordance with the 3Rs principles