The document outlines the CPCSEA guidelines for animal facilities, including their objectives, functions, and regulatory mechanisms in India. It emphasizes the moral responsibilities of researchers towards laboratory animals, highlights the importance of proper anesthesia and euthanasia methods, and details functional requirements for animal housing. The document also discusses the significance of environmental control and standard operating procedures for maintaining animal welfare in research settings.

1. CPCSEA GUIDELINES FOR
ANIMAL HOUSE
Presented by-
Vasanti Patil
M.Pharm First Year
Quality assurance

2. CONTENTS
INTRODUCTION
OBJECTIVE
WHY ANIMALS ARE USED IN RESEARCH
CPCSEA
COMPOSITION OF COMMITTEE
OBJECTIVES OF CPCSEA
FUNCTIONS OF CPCSEA
FUNCTIONAL REQUIREMENTS OF ANIMAL FACULTY
LOCATION OF ANIMAL FACILITIES TO LABOROTARIES
SOP AND GUIDELINES
ANESTHESIA
VARIOUS ANESTHETIC AGENTS USED FOR ANAESTHESIA
EUTHANASIA
 REGULATORY MECHANISM OF CPCSEA

3. WHY ANIMALS ARE USED IN RESEARCH?
•To understand the principles of anatomy and physiology
which are true for all mammals.
•Certain strains and breeds of animal get the same disease or
as condition as human.
•It is possible to control environment (temperature and
humidity etc.) of animals and shield them from disease not
related to research.
•We can scientifically study physiology by using valid no of
animal.

4. C0mmitee for the purpose of control and
supervision.(CPCSEA)
Statutory body formed by the Act of indian
parliament under the prevention to cruelty to animal
act,1960.
Formed in 1964.
Revived in 1998 under the committed
chairpersonship of Menka Gandhi.
Head quarters at Chennai .Includes various
guidelines and subcommittees.

5. National level expert committee to scrutinise
and approve the research projects conducting
using animals.
Introduce a national “good laboratory practice”
document.
Introduce the credo of ‘3R principles’.
2004 –CPCSEA officially accept the concept of
4th R.
“Investigator have moral responsibility to take
care of laboratory animals after use”.

6. This committee is composed of:-
•Regulatory authority
•Member of scientific community.
•Animal activist
•The committee has 10 experts and 3 official
members.

7. OBJECTIVES :-
•To avoid unnecessary pain before, during and after
experiment.
•To promote human care of animal used in research and
testing.
•To provide guideline for anesthesia , euthanasia, housing ,
care, breeding, and maintenance.
FUNCTION:-
• Approval of Animal House Facility on the basis of
inspection conducted by CPCSEA.
•Recommendation for import of animal used in experiments.
•Action against in case of violation any legal norm.
•Permission for conducting experiments.

8.  Veterinary care
Animal procurement
Quarantine ,separation and Stabilization
 Transport of animal
Servillince , Diagnosis, Treatment of disease
Laboratory animal Husbandry and management
Food and water
Bedding
Animal care and technical personnel
Sanitation and cleanliness
Duration of experiment
Personal hygiene
Caging and housing system

9. Restraint
Waste disposal
Emergency ,Weekend ,Holiday care
Location of animal facilities to laboratoris
Functional area requirement of animal facility

10. FUNCTIONAL AREA REQUIREMENT
OF ANIMAL FACILITY
Building material
Corridor
Utilities
Animal room doors
Exterior window
Floors
Walls and ceilings
Storage area
Experimental area
Environment
Sanitizing equipment

11. Location Of Animal Facilities to laboratories
Animal house should be located in isolated building far away
from human habitations and not exposed to dust , smoke
,noise, wild rodents and birds.
Housing area should be near to laboratories
The space should be well divided for various activities .
1. The animal room should occupy about 50-60% of total
constructed area .
2. 40 to 50 % area for store, washing, office and staff ,
machine room ,quarantine and corridors .
 Since animals are very sensitive to environmental changes
,sharp fluctuation in temperature ,Humidity ,Light , Sound
and Ventilation should be avoided.

12. STANDERD OPERATING PROCEDURE
AND GUIDELINES
SOP should contain following items
1. Name of author
2. Title of SOP
3. Date of approval
4. Reference of previous SOP on same subject and date (Issue
number and date )
5. Location and distribution of SOP s with sign of each
recipient
6. Objective
7. Detailed info of the instrument used in relation with
animals with methodology .

13. 8. The name of manufacturer of the reagents
and methodology of analysis pertaining to
animals
9. Normal value of all parameters
10. Hazards identification and risk assesment

14. Environment .
 Temperature
 Humidity control
 Ventilation
 Power and Lighting
 Noise control
 Between 18-29 celcious
 Range 30-70
 Design with 112-15 air
cycles per hour
 System should be safe
 Fluorescent lights are
eeficient
 Emergency backup needed
 Noise free envioronment
 Concrete walls are more
effective

15. Food and Water
Feed should contain Feed should not contain
Moisture insecticide ,hormones
,antibiotics
crude fiber,crude fat
,crude protein
Potential toxicants
Essential bits Heavy metal

16. Anesthesia
 Before use of actual anesthetic , the animal should be
prepared for anesthesia by overnight fasting and using pre
anesthetic .
 Local anesthetic are used to block the nerve supply to a
limited area and should be used only for rapid and minor
procedure .
 For general anesthetic agents are used in the form of
inhalants , intravenous and intra -ocular type of injections.
The animal should remain in veterinary care till it completely
recover from anesthesia

17. COMMNLY USED ANESTHETIC AGENT FOR LABORATARY ANIMAL
Drug
Mg/ml
mouse rat Guinea
pig
KETAMINE HCL 22-24 im - 22-24 im
PENTO BARBITONE
SODIM
35 Iv
50 ip
35 iv
40 ip 30 iv
THIOPENTONE
SODIUM
25 iv
50 ip
20 iv
40 ip
20 iv
55 ip
URETHANES - 0.75 ip 1.5 ip

18. Euthanasia
In the event of decision to sacrifice the animal on
termination of the experiment or otherwise , an
approved method of euthanasia should be adopted.
The procedure should be carried out quickly and
painlessly in an atmosphere free from anxiety and fear

19. Methods used for euthanasia should meet the
following requirements :
Death , without causing anxiety , pain or distress
with minimum time lag phase
Compatibility with purpose of study and minimum
emotional effects on operator .
Location should be separate from animal room and
free from environmental contaminants .
Minimum physiological and psychological
disturbances .
The investigator must ensure that animal is
clinically dead before it is sent for disposal .

20. Euthanasia of Laboratory Animals
a) INHALATIONAL GASES
Carbon Monoxide
Carbon Dioxide
Carbon Dioxide + chloroform
Halothane
b) DRUG ADMINISTRATION
Barbiturate overdose (Route IP)
Chloral hydrate overdose (Route IP)
ketamine overdose
sodium pentothol overdose
c) PHYSICAL METHODS
Eloctrocution
Decapitation (For analysis of stress)
Cervical dislocation

21. Regulatory Mechanism of CPCSEA
1)CPCSEA- committee for the purpose of control
and supervision of experiments on animals .
2)Sub –committees of the CPCSEA
3)Sub committee on large animal (SCLA)
4)Sub committee for selection of CPCSEA
Nominees on institutional Animal ethics
committee (IAEC)

22. REFERENCES
 http://icmr.nic.in /animal _ethics.htm
cpcsea.nic .in
Google.com: Operational Guidelines
for Observance of Good Practices in
CPCSEA
www.aaalac.org /resource/SOP

23. THANK YOU