This study evaluated the use of an Accutome AccuPach VI pachymetry system to measure corneal thickness in cynomolgus monkeys. The system showed good intra-run precision between 5.3-6.9% and inter-run precision between 1.5-1.9%. Measurements also showed high concordance between technicians of 97.9-98.3%. The mean corneal thickness measurements of 411.3-418.7 μm were within the normal expected range for cynomolgus monkeys using ultrasound pachymetry. The pachymetry system was determined to be valid for measuring corneal thickness in cynomolgus monkeys based on the precision and accuracy of the measurements obtained.