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Sofía López Valencia
Universidad Pontificia Bolivariana
Facultad de Medicina- Biologia
molecular tercer semestre
The giant Malaysian shrimp
Shrimp in the family Palaemonidae in the order
Decapoda, invertebrate
Tropical Asia, Latin America and Africa
It feeds on parasites and dead tissues, can
cause a negative impact on effluents
More than 30 cm, it is the largest shrimp
farmed in the world
The coloration is green or blue with black
stripes.
Macrobrachium rosenbergii
Immune response of invertebrate
organisms such as crustaceans and
insects.
Capable of binding to LPS found in the
membranes of gram-negative bacteria, as
well as beta-1,3-glucans
Acts as a receptor of pathogens,
recognizing the presence of these
microbial components and triggering
defensive responses
Lipopolysaccharide‑ and Beta‑1,3‑Glucan Binding Protein (LGBP)
Characterization of a Lipopolysaccharide‑ and Beta‑1,3‑Glucan
Binding Protein (LGBP) from the Hepatopancreas of Freshwater
Prawn, Macrobrachium rosenbergii, Possessing Lectin‑Like
Activity
General objective
Methods
Native Gradient PAGE Analysis
Gradient of a mild detergent-->
allows proteins to be
separated--> without affecting
their native state
This procedure is used to
separate and determine the
molecular weight of the
proteins in the purified lectin
sample.
SDS‑PAGE Analysis
Protein solution mixed with an
anionic detergent --> eliminating
their secondary and tertiary
structures
This procedure is used to
separate and determine the
molecular weight of the lectin in
the purified lectin sample.
Methods
Sandwich ELISA
Technique that is used when
specific antibodies are available
for both the capture and
detection of the protein of
interest
Quantify the concentration of a
protein called lectin in
hepatopancreas and serum
samples of M. rosenbergii.
Technique used to detect and
analyze specific proteins in a
given sample
The detailed process used to
improve the specificity of the
polyclonal antiserum as well as
the verification of the specificity
of the labeled antibody by
Western blot.
Western Blot
Results
Results
Discussion
The study of lectins, particularly LGBP, in the immune response of
crustaceans provides a crucial scientific basis with practical applications in
the development of therapies, vaccines, diagnostic tools and disease
control strategies in aquaculture, as well as in identification of biomarkers
to evaluate the health and immune response of crustaceans and
potentially other organisms.
Biomarkers identified in studies on LGBP expression could be used to
monitor the immune response of human patients to bacterial infections or
other immunological challenges. This could provide important information
about disease progression and the effectiveness of treatments
Conclusions
seminario bio mol- sofia lopez valenciaa

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seminario bio mol- sofia lopez valenciaa

  • 1. Sofía López Valencia Universidad Pontificia Bolivariana Facultad de Medicina- Biologia molecular tercer semestre
  • 2. The giant Malaysian shrimp Shrimp in the family Palaemonidae in the order Decapoda, invertebrate Tropical Asia, Latin America and Africa It feeds on parasites and dead tissues, can cause a negative impact on effluents More than 30 cm, it is the largest shrimp farmed in the world The coloration is green or blue with black stripes. Macrobrachium rosenbergii
  • 3. Immune response of invertebrate organisms such as crustaceans and insects. Capable of binding to LPS found in the membranes of gram-negative bacteria, as well as beta-1,3-glucans Acts as a receptor of pathogens, recognizing the presence of these microbial components and triggering defensive responses Lipopolysaccharide‑ and Beta‑1,3‑Glucan Binding Protein (LGBP)
  • 4. Characterization of a Lipopolysaccharide‑ and Beta‑1,3‑Glucan Binding Protein (LGBP) from the Hepatopancreas of Freshwater Prawn, Macrobrachium rosenbergii, Possessing Lectin‑Like Activity General objective
  • 5. Methods Native Gradient PAGE Analysis Gradient of a mild detergent--> allows proteins to be separated--> without affecting their native state This procedure is used to separate and determine the molecular weight of the proteins in the purified lectin sample. SDS‑PAGE Analysis Protein solution mixed with an anionic detergent --> eliminating their secondary and tertiary structures This procedure is used to separate and determine the molecular weight of the lectin in the purified lectin sample.
  • 6. Methods Sandwich ELISA Technique that is used when specific antibodies are available for both the capture and detection of the protein of interest Quantify the concentration of a protein called lectin in hepatopancreas and serum samples of M. rosenbergii. Technique used to detect and analyze specific proteins in a given sample The detailed process used to improve the specificity of the polyclonal antiserum as well as the verification of the specificity of the labeled antibody by Western blot. Western Blot
  • 10. The study of lectins, particularly LGBP, in the immune response of crustaceans provides a crucial scientific basis with practical applications in the development of therapies, vaccines, diagnostic tools and disease control strategies in aquaculture, as well as in identification of biomarkers to evaluate the health and immune response of crustaceans and potentially other organisms. Biomarkers identified in studies on LGBP expression could be used to monitor the immune response of human patients to bacterial infections or other immunological challenges. This could provide important information about disease progression and the effectiveness of treatments Conclusions