Santos et al 2010 Chr Res
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This document summarizes a study that mapped 72 dinucleotide microsatellite loci from Drosophila subobscura onto its polytene chromosomes using fluorescent in situ hybridization (FISH). The distribution of microsatellites was not uniform across chromosomes, with higher density in the sex chromosome than autosomes. Homologous regions were identified in D. pseudoobscura and D. melanogaster genomes, supporting conservation of chromosomal elements among Drosophila species but also intrachromosomal rearrangements within lineages. The lack of microsatellite repeats in homologous D. melanogaster sequences suggests convergent evolution for high density in the X chromosome distal region.
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It was a coincidence that during panel discussions I came across team from Deloitte and C44, instrumental in preparation of NTK's winning Smart City Proposal. For me it was one of the happiest moment for being in the same panel with current mentors, as I was involved with the NTK project from pre-takeoff to maturity stage.
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The structure of AA1D was designed to be resilient as it was meant to promote (a) Compact densification; (b) Scaling of a Traditional neighbourhood; (c) Fine Grain diversity
I have also highlighted the fact that what we have done in (terms of TND design) more than 1.5 decades back in the year 2000-01, is being replicated now as a Brownfeild Retrofit by Global Smart City Barcelona through creation of SMART SUPER BLOCKS ( we were ahead of time).
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https://www.linkedin.com/pulse/greenfield-urban-retrofit-inspired-traditional-design-choudhury?trk=pulse_spock-articles
ALADI
La Asociación Latinoamericana de Integración (ALADI) es una organización internacional formada por 13 países latinoamericanos con el objetivo de lograr la integración económica en la región a través del establecimiento de un mercado común. La ALADI sustituyó a la Asociación Latinoamericana de Libre Comercio (ALALC) en 1980 y persigue sus objetivos mediante preferencias arancelarias regionales, acuerdos regionales y acuerdos de alcance parcial entre países miembros.
Power plant control
This document discusses a close loop control system and DDCMIS (Distributed Control and Data Collection and Management Information System). It describes the DDCMIS system as having three levels: process I/O, process control, and management. It then lists common instrumentation used in power plants including temperature, pressure, level, flow, turbine, steam/water analyzers, and gas analyzers. The document shows how these instruments connect to the DDCMIS through a control network. It briefly mentions DDCMIS options and connectivity between the plant and management systems. Finally, it provides examples of process control systems in power plants such as coordinated master control, air and fuel flow control, and drum level/feed water control.
How to select linear actuators for lab instruments
This document discusses various electromechanical drive train options for lab instruments, including ball screws, lead screws, timing belts, rack and pinions, and linear motors. It compares the technologies on factors like precision, repeatability, accuracy, speed, efficiency, expected life, and special considerations. Ball screws are best for high precision applications requiring good repeatability. Timing belts are maintenance-free and ideal for high speeds. Linear motors provide very high precision and speed but can be expensive. The document provides advice on selecting the right option and notes that Parker can help achieve the optimal balance through both standard and custom engineered solutions.
Negotiable instruments
The document discusses various aspects of negotiable instruments law in India including:
1) It defines key negotiable instruments like promissory notes, bills of exchange, and cheques.
2) It explains the requirements for an instrument to be considered negotiable as well as distinguishing features of a holder versus a holder in due course.
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Systemische Professionalität - Dilemma, Sinn und komplexe Wirklichkeiten
Systemische Professionalität - Dilemma, Sinn und komplexe Wirklichkeitenisb GmbH Systemische Professionalität
Kernsapekte:
- Dilemma: Eingebaute Unlösbarkeit
- Innerhalb Dilemmalogik keine oder nur unbefriedigende Lösungen
- Dilemmagleichungen: Bedeutungsgebungen konstituieren Unlösbarkeit
- Dilemmainszenierungen: Verschiedene Inszenierungen auf der selben Bühne; Inszenierungen nach unterverträglichen, konkurrierenden Gesichtspunkten, Mehrfachzugriff auf die selben Ressourcen;
- Dilemmazirkel: Strampeln (Aktivität ohne Zuversicht); Erschöpft abschalten (Pause ohne Neubelebung); Verzweifeln; Vermeiden/ Leugnen
- Chancenreiche Haltungen:
- Verzweifeln als Professionelle Kompetenz: Bei Zeiten loslassen können
Hyperlinks zu youtube*:
Link Slide #1: http://www.youtube.com/watch?v=84JVEw0a6r4&t=0m
Link Slide #2: http://www.youtube.com/watch?v=84JVEw0a6r4&t=16m20s
Link Slide #3: http://www.youtube.com/watch?v=84JVEw0a6r4&t=20m10s
*Slideshare verhindert Hyperlinks auf den ersten drei Folien.Systemische Professionalität - Verantwortung und Macht
Kernaspekte:
- Verantwortung:
- Dimensionen von Verantwortung (Qualifikation, Wertorientierung, Zuständigkeit, Autorisierung)
- Komplementäre Verantwortung (Verantwortung "für"/ Verantwortung "in Bezug auf")
- Störungen im Verantwortungssystem
Hyperlinks zu youtube*:
Link Slide #1: http://www.youtube.com/watch?v=G9s5SUHkBv8&t=24m40s
Link Slide #2: http://www.youtube.com/watch?v=G9s5SUHkBv8&t=26m
Link Slide #3: http://www.youtube.com/watch?v=G9s5SUHkBv8&t=28m30s
*Slideshare verhindert Hyperlinks auf den ersten drei Folien.
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Systemische Professionalität - Dilemma, Sinn und komplexe Wirklichkeiten
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Microsatellites- Molecular fingerprints
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Molecular markers for measuring genetic diversity
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Introduction:
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Presentation1..gymno..non specific markers n microsatellites..by Nikita Patha...
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Santos et al 2010 Chr Res
- 1. FISH mapping of microsatellite loci from Drosophila subobscura and its comparison to related species Josiane Santos & Lluis Serra & Elisabet Solé & Marta Pascual Received: 9 October 2009 /Revised: 6 January 2010 /Accepted: 8 January 2010 /Published online: 3 March 2010 # Springer Science+Business Media B.V. 2010 Abstract Microsatellites are highly polymorphic markers that are distributed through all the genome being more abundant in non-coding regions. Whether they are neutral or under selection, these markers if localized can be used as co-dominant molecular markers to explore the dynamics of the evolutionary processes. Their cytological localization can allow identifying genes under selection, inferring recombination from a genomic point of view, or screening for the genomic reorganizations occurring during the evolution of a lineage, among others. In this paper, we report for the first time the localization of microsatellite loci by fluorescent in situ hybridization on Drosophila polytene chromosomes. In Drosophila subobscura, 72 dinucleo- tide microsatellite loci were localized by fluorescent in situ hybridization yielding unique hybridization signals. In the sex chromosome, microsatellite distribution was not uniform and its density was higher than in autosomes. We identified homologous segments to the sequence flanking the microsatellite loci by browsing the genome sequence of Drosophila pseudoobscura and Drosophila melanogaster. Their localization sup- ports the conservation of Muller’s chromosomal ele- ments among Drosophila species and the existence of multiple intrachromosomal rearrangements within each evolutionary lineage. Finally, the lack of microsatellite repeats in the homologous D. melanogaster sequences suggests convergent evolution for high microsatellite density in the distal part of the X chromosome. Keywords cytogeneticmap . Drosophila . fluorescentin situ hybridization . genome rearrangement . microsatellite loci . repetitive DNA . genome comparison Abbreviations DAPI 4'-6-Diamidino-2-phenylindole dsub ID names of the clones FISH fluorescent in-situ hybridization HP1 Heterochromatin Protein 1 NTP nucleotides triphosphate PCR polymerase chain reaction rDNA ribosomal DNA SSC Sodium Chloride-Sodium Citrate buffer SSR simple sequence repeats UCSC University of California Santa Cruz Introduction Microsatellites are highly polymorphic markers consti- tuted by simple sequence tandem repeats (SSR) of one to six nucleotides per unit (Bachtrog et al. 1999). They are ubiquitous among eukaryotes and have been described from a great variety of taxa (Wilder et al. 2002), being generally considered neutral unless linked to loci under strong selection. Although SSRs typically Chromosome Research (2010) 18:213–226 DOI 10.1007/s10577-010-9112-4 Responsible Editor: Pat Heslop-Harrison. J. Santos :L. Serra :E. Solé :M. Pascual (*) Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain e-mail: martapascual@ub.edu J. Santos CBA, Departamento de Biologia, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisbon, Portugal