This document provides guidelines for the safe preparation, storage, and handling of powdered infant formula. Powdered infant formula is not a sterile product and may contain pathogens like Enterobacter sakazakii and Salmonella enterica that can cause serious illness in infants. Reconstituting powdered infant formula with water heated to at least 70°C and minimizing the time between preparation and consumption are recommended to reduce risks. Separate guidelines are provided for care settings and home environments. The recommendations aim to minimize risks while allowing for the use of powdered infant formula when breastfeeding is not possible.
The document defines and describes the crash cart, which contains emergency equipment and medications needed to treat cardiac emergencies. It outlines the crash cart's history, contents, and proper arrangement according to hospital policy. The crash cart must be checked regularly by nurses to ensure it is fully stocked and functional. It provides a mobile station containing defibrillators, airway equipment, intravenous drugs, and other supplies to quickly respond to life-threatening situations.
This document discusses diabetes and its management. It states that body cells require glucose for energy production, which enters cells through insulin. Without enough insulin, glucose levels in blood increase, potentially causing vision problems, kidney damage, heart attacks and other issues. Proper diet, exercise, medication and monitoring are recommended for diabetes management and prevention of complications. It provides guidance on food choices, exercise, herbal remedies and lifestyle practices to control blood sugar levels.
The document discusses India's cold chain system for transporting and storing vaccines. It defines the cold chain as maintaining vaccines at recommended temperatures from manufacture to point of use. Key components of the cold chain system include equipment like walk-in freezers and coolers, refrigerators, cold boxes, vaccine carriers and day carriers. Vaccines are stored at different levels from national stores down to local health centers. Proper temperature maintenance is important to preserve vaccine potency and effectiveness of immunization programs.
Rabies is a fatal viral disease transmitted through the saliva of infected mammals. It causes acute inflammation of the brain and is nearly 100% fatal in humans if left untreated. Dogs are the primary source of human rabies infections. Post-exposure prophylaxis, consisting of wound cleansing, rabies immune globulin injection, and a vaccine series, is highly effective in preventing the disease if administered promptly after exposure. No approved treatments exist once symptoms develop, making vaccination an important part of rabies prevention.
Health talk on immunization ( presentation, health talk, lesson plan )SADDAM HUSSAIN
A health talk topic on immunization for nursing student .
also usefull for reading and improve knowledge
community health nursing , msc nursing , bsc nursing.
The document provides guidance on bottle feeding for new parents. It discusses the necessary equipment for bottle feeding including the number of bottles, types of bottles and teats, and sterilization equipment. It also provides instructions on how to properly bottle feed an infant, including how to hold the baby, encourage latching, ensure the teat is full of milk, and burp the baby afterwards. The document emphasizes the importance of sterilizing all bottle feeding equipment before each use to prevent bacterial infection in infants.
The document defines and describes the crash cart, which contains emergency equipment and medications needed to treat cardiac emergencies. It outlines the crash cart's history, contents, and proper arrangement according to hospital policy. The crash cart must be checked regularly by nurses to ensure it is fully stocked and functional. It provides a mobile station containing defibrillators, airway equipment, intravenous drugs, and other supplies to quickly respond to life-threatening situations.
This document discusses diabetes and its management. It states that body cells require glucose for energy production, which enters cells through insulin. Without enough insulin, glucose levels in blood increase, potentially causing vision problems, kidney damage, heart attacks and other issues. Proper diet, exercise, medication and monitoring are recommended for diabetes management and prevention of complications. It provides guidance on food choices, exercise, herbal remedies and lifestyle practices to control blood sugar levels.
The document discusses India's cold chain system for transporting and storing vaccines. It defines the cold chain as maintaining vaccines at recommended temperatures from manufacture to point of use. Key components of the cold chain system include equipment like walk-in freezers and coolers, refrigerators, cold boxes, vaccine carriers and day carriers. Vaccines are stored at different levels from national stores down to local health centers. Proper temperature maintenance is important to preserve vaccine potency and effectiveness of immunization programs.
Rabies is a fatal viral disease transmitted through the saliva of infected mammals. It causes acute inflammation of the brain and is nearly 100% fatal in humans if left untreated. Dogs are the primary source of human rabies infections. Post-exposure prophylaxis, consisting of wound cleansing, rabies immune globulin injection, and a vaccine series, is highly effective in preventing the disease if administered promptly after exposure. No approved treatments exist once symptoms develop, making vaccination an important part of rabies prevention.
Health talk on immunization ( presentation, health talk, lesson plan )SADDAM HUSSAIN
A health talk topic on immunization for nursing student .
also usefull for reading and improve knowledge
community health nursing , msc nursing , bsc nursing.
The document provides guidance on bottle feeding for new parents. It discusses the necessary equipment for bottle feeding including the number of bottles, types of bottles and teats, and sterilization equipment. It also provides instructions on how to properly bottle feed an infant, including how to hold the baby, encourage latching, ensure the teat is full of milk, and burp the baby afterwards. The document emphasizes the importance of sterilizing all bottle feeding equipment before each use to prevent bacterial infection in infants.
Nurses’ role in empowering smallholder women in developing countriesRobert Parolin
Smallholder women in developing countries face unique health and economic challenges. They make up the majority of farmers but have less access to resources and face increased risks of illness. Nurses can help empower smallholder women by supporting primary healthcare integrated with rural development, and by advocating for women's agricultural groups which improve access to resources, markets, and policy change. Working with these groups within a framework of rural development and primary healthcare can address the social determinants of health and livelihoods of smallholder women.
The document discusses prosthesis and elder abuse. It defines geriatric nursing and describes different types of prosthesis including dental and hearing aids. It outlines the types, signs, and risk factors of elder abuse such as physical, emotional, sexual, and financial abuse. The document also discusses who typically commits elder abuse and ways to prevent and raise awareness of elder abuse such as educating caregivers, visiting older loved ones, and reporting any suspicions of abuse.
The document discusses the differences in illness between children and adults and outlines guidelines for admitting sick children to the hospital, including preparing the room and family for admission. It also examines children's reactions to hospitalization according to their developmental stage and the roles of nurses in helping children and their families cope with and adapt to being in the hospital.
This document discusses the various roles of a nurse in maternal and child health programs in India. It outlines that nurses serve as service providers by ensuring essential newborn and child care services. They also serve as administrators by developing policies related to maternal and child health. Additionally, nurses take on educational roles by organizing training programs and designing health education materials. Nurses also serve as researchers by identifying areas for research and supporting research activities in maternal and child health.
The document discusses various methods for preventing diarrhoea and acute respiratory infections. It recommends improved sanitation through access to clean water and proper excreta disposal. Maintaining hygiene through hand washing, especially before eating and after using the latrine, is essential. Exclusive breastfeeding for six months and continuing breastfeeding with proper weaning can protect against diarrhoea. Immunization, zinc supplementation, and avoiding overcrowding can also help prevent diarrhoea and respiratory infections. Maintaining indoor air quality through ventilation, avoiding indoor pollution and drinking fluids can reduce respiratory infections.
Prevention & control of malnutrition, st is & cancer........Public Health Update
The document summarizes strategies for preventing malnutrition, sexually transmitted infections, and cancer. It discusses prevention at various levels including family, community, national, and international. For malnutrition, it recommends exclusive breastfeeding, nutritional supplementation, and treating infections early. For STIs, it suggests health education, screening, contact tracing, and condom promotion. For cancer, primary prevention includes a healthy diet, exercise, avoiding tobacco and alcohol, vaccination, and reducing environmental toxins and radiation. Secondary prevention involves cancer screening and early detection.
Essential newborn care (ENC) should be provided to all newborns and includes maintaining warmth, initiating breastfeeding within 1 hour of birth, eye care, vitamin K administration, and immunizations. Immediate newborn care involves drying the baby, maintaining temperature, establishing breathing, vitamin K injection, Apgar scoring, and initiating breastfeeding. Routine newborn care consists of maintaining warmth, exclusive breastfeeding, skin and cord care, eye care, and monitoring growth through measurements.
The document discusses the cold chain system used for storing vaccines. It defines cold chain as a series of links that keep vaccines within the recommended temperature range from manufacture to administration. Key aspects of cold chain discussed include various equipment used like walk-in cold rooms, deep freezers, ice-lined refrigerators, cold boxes, vaccine carriers and ice packs. The document outlines the temperature requirements for different vaccines and nurses' responsibility in maintaining the cold chain to ensure vaccine potency.
Effective Diet plan for diabetic patientMedisys Kart
Diet Plays major role in diabetes. If you are not taking your diet properly and eating foods that should be avoided then it can be dangerous. So we are sharing some of the diet tips for diabetic patient in ppt so that you can eat right food and get rid of diabetes.
HYPERTENSION IN COMMUNITY HEALTH NURSINGHarsh Rastogi
Hypertension is a common cardiovascular disorder that poses a major public health challenge. It is defined as elevated blood pressure and is a major risk factor for cardiovascular mortality. It is classified as primary or secondary, with primary hypertension accounting for 90% of cases and having generally unknown causes. Risk factors include age, genetics, obesity, diet high in salt and saturated fat, physical inactivity, and stress. Prevention strategies focus on lifestyle modifications like healthy diet, exercise, weight control, limiting alcohol and reducing sodium intake.
Cold chain refers to the process of maintaining optimal temperature conditions for vaccines from manufacturer to administration. It involves receipt, storage, and delivery of vaccines using various equipment like cold boxes, vaccine carriers, refrigerators, and freezers. Temperature must be monitored daily and vaccines discarded if exposed to unsuitable temperatures as this impacts potency. Proper estimation, documentation, and emergency procedures are important for ensuring vaccine quality during transportation and storage in the cold chain.
KIDNEY AND DIET
KIDNEY ARE THE IMPORTANT ORGANS OF THE BODY.
THESE HELP TO FLUSH OUT THE HARMFUL PRODUCTS FROM THE BODY.
THESE MAKE A VERY IMPORTANT PART OF URINARY TRACT SYSTEM .
It is advise that the patient suffering from kidney failure,must minimize the consumption of food items,which are rich in potassium, sodium and proteins.
WWW.KARMAAYURVEDA.COM
.
Instead,they must consume food products which are rich in anti-oxidants,vitamins,phytochemicals and flavanoids.
WWW.KARMAAYURVEDA.COM
DIET IN KIDNEY FALIOUR
The patients with kidney failure must pursue a strict diet regime to lead a healthy life and avoid further complications. Following is a diet chart, which the patients suffering from kidney failure must follow.
FOOD PRODUCTS TO BE AVOID
PULSES(rich in proteins and potassium content)
×soybeans
× winged beans
× black beans
× white beans
FOOD PRODUCTS TO BE AVOID
WWW.KARMAAYURVEDA.COM
FOOD PRODUCTS TO BE AVOID
WWW.KARMAAYURVEDA.COM
FOOD PRODUCTS TO BE AVOID
WWW.KARMAAYURVEDA.COM
FOOD PRODUCTS TO BE AVOID
Generally all the spices contain approximately more than 50 percent of potassium in them.
So , it is recommended to avoid much consumption of spices of any from.
Use salt in very less quality.
Don’t use tablets salts.
OTHER FOODS PRODUCTS
FOOD PRODUCTS TO BE COMSUMED
WWW.KARMAAYURVEDA.COM
FOOD PRODUCTS TO BE COMSUMED
FOOD PRODUCTS TO BE CONSUMED
VEGETABLES
FOOD PRODUCTS TO BE CONSUMED
CARDAMOM
BLACKPEPPER
SUNFLOWER OIL
OLIVE OIL
CORIANDER
MINT
BAYLEAVES
FRNUGREEK/METHI
FOOD PRODUCTS TO BE CONSUMED
MILK-LOW FAT
MUNGDAL KHICHADI
WHEAT
RICE
THE PATIENT SUFFERING FROM KIDNEY FALIOUR ARE STRICTLY ADVISE TO FOLLO THE ABOVE DIET CHART PROPERLY.
THIS DIET CHART IS PREPARED BASED ON THE NUTRITIONAL CONTENT CARBOHYDRATES , PROTEINS ,FATS AND VITAMINS
The document discusses key aspects of the Children Act 1989 in the UK. It establishes that a child's welfare is the paramount consideration in legal decisions. It defines parental responsibility and outlines the duties of local authorities to investigate child welfare concerns and protect children, including through care and supervision orders if needed. The Act aims to reform laws around protecting children's rights and well-being.
This document discusses the importance of nutrition education programs in developing countries. It notes that lack of awareness about dietary requirements and food nutrition is a major cause of malnutrition. Nutrition education should be practical and tailored to the local socioeconomic conditions, food habits, and available food resources. The goals of nutrition education programs are to increase awareness, enhance motivation, facilitate actions to improve nutrition, and improve environmental support. Common methods of nutrition education discussed include lectures, demonstrations, workshops, films, posters, printed materials, radio, and television.
This document outlines important nursing diagnoses for several common conditions:
1. For fever, key diagnoses include hyperthermia, risk for dehydration, knowledge deficit about the disease, and anxiety. Sleep may also be disturbed.
2. For diabetes, important diagnoses are knowledge deficit about managing the disease, risk for unstable blood sugar, risk for infection, and activity intolerance from hypo/hyperglycemia. Anxiety is also common.
3. For hypertension, risk for decreased cardiac output, acute headache pain, ineffective tissue perfusion, knowledge deficit, and anxiety are key.
4. For appendectomy, acute pain, risk for fluid deficit, risk for infection, knowledge deficit, and anxiety are the major nursing
A crash cart or code cart (crash trolley in UK medical jargon) or "MAX cart" is a set of trays/drawers/shelves on wheels used in hospitals for transportation and dispensing of emergency medication/equipment at the site of medical/surgical emergency for life support protocols to potentially save someone's life.
This document provides information on newborn characteristics, assessment, and care. It describes the typical physical attributes of newborns such as weight, length, skin appearance. Vital signs at birth and how they adjust in the first hours are outlined. The document emphasizes the importance of thoroughly assessing the newborn to identify any issues and ensure healthy transition after birth. Key parts of physical exam and Apgar scoring are explained. Guidelines for routine newborn care like cleaning, feeding, and rooming-in with mothers are provided.
This document provides information on nursing care for patients undergoing dialysis. It discusses protocols for hemodialysis and peritoneal dialysis. Hemodialysis requires vascular access, a dialysis machine, dialysate solution, and takes place over 12 hours per week divided into 3 sessions. Complications can include infection, blood clotting, and hypotension. Nursing considerations include medication management, access site care, monitoring for complications, and providing education and psychological support. Peritoneal dialysis can cause discomfort and complications like peritonitis. Special care is needed for hospitalized dialysis patients to prevent infections and control electrolyte levels.
An enema is the introduction of fluid into the lower bowel through the rectum to cleanse or administer medication. There are several types of enemas that serve different purposes such as treating constipation, softening stool, administering medication, or nourishing patients. The document outlines various enema solutions used, including soap and water, oil, glycerin, and medications. It also describes procedures for administering different types of enemas and important safety considerations.
The document discusses the benefits of exercise for mental health. Regular physical activity can help reduce anxiety and depression and improve mood and cognitive function. Exercise causes chemical changes in the brain that may help alleviate symptoms of mental illness and boost overall mental well-being.
Multinationals are organizations headquartered in one country that operate in two or more other countries. They have advantages like taking advantage of grants, increasing market share, and cutting costs through cheaper labor. However, they also have disadvantages like paying tax in multiple countries and ensuring they observe local traditions. For the countries they operate in, multinationals can create jobs and introduce new technologies, but can also have too much influence and send profits back to their home country. The document provides an outline for a task analyzing a specific multinational company in terms of its profile, reasons for being multinational, and advantages/disadvantages for the company and countries it operates in.
Nurses’ role in empowering smallholder women in developing countriesRobert Parolin
Smallholder women in developing countries face unique health and economic challenges. They make up the majority of farmers but have less access to resources and face increased risks of illness. Nurses can help empower smallholder women by supporting primary healthcare integrated with rural development, and by advocating for women's agricultural groups which improve access to resources, markets, and policy change. Working with these groups within a framework of rural development and primary healthcare can address the social determinants of health and livelihoods of smallholder women.
The document discusses prosthesis and elder abuse. It defines geriatric nursing and describes different types of prosthesis including dental and hearing aids. It outlines the types, signs, and risk factors of elder abuse such as physical, emotional, sexual, and financial abuse. The document also discusses who typically commits elder abuse and ways to prevent and raise awareness of elder abuse such as educating caregivers, visiting older loved ones, and reporting any suspicions of abuse.
The document discusses the differences in illness between children and adults and outlines guidelines for admitting sick children to the hospital, including preparing the room and family for admission. It also examines children's reactions to hospitalization according to their developmental stage and the roles of nurses in helping children and their families cope with and adapt to being in the hospital.
This document discusses the various roles of a nurse in maternal and child health programs in India. It outlines that nurses serve as service providers by ensuring essential newborn and child care services. They also serve as administrators by developing policies related to maternal and child health. Additionally, nurses take on educational roles by organizing training programs and designing health education materials. Nurses also serve as researchers by identifying areas for research and supporting research activities in maternal and child health.
The document discusses various methods for preventing diarrhoea and acute respiratory infections. It recommends improved sanitation through access to clean water and proper excreta disposal. Maintaining hygiene through hand washing, especially before eating and after using the latrine, is essential. Exclusive breastfeeding for six months and continuing breastfeeding with proper weaning can protect against diarrhoea. Immunization, zinc supplementation, and avoiding overcrowding can also help prevent diarrhoea and respiratory infections. Maintaining indoor air quality through ventilation, avoiding indoor pollution and drinking fluids can reduce respiratory infections.
Prevention & control of malnutrition, st is & cancer........Public Health Update
The document summarizes strategies for preventing malnutrition, sexually transmitted infections, and cancer. It discusses prevention at various levels including family, community, national, and international. For malnutrition, it recommends exclusive breastfeeding, nutritional supplementation, and treating infections early. For STIs, it suggests health education, screening, contact tracing, and condom promotion. For cancer, primary prevention includes a healthy diet, exercise, avoiding tobacco and alcohol, vaccination, and reducing environmental toxins and radiation. Secondary prevention involves cancer screening and early detection.
Essential newborn care (ENC) should be provided to all newborns and includes maintaining warmth, initiating breastfeeding within 1 hour of birth, eye care, vitamin K administration, and immunizations. Immediate newborn care involves drying the baby, maintaining temperature, establishing breathing, vitamin K injection, Apgar scoring, and initiating breastfeeding. Routine newborn care consists of maintaining warmth, exclusive breastfeeding, skin and cord care, eye care, and monitoring growth through measurements.
The document discusses the cold chain system used for storing vaccines. It defines cold chain as a series of links that keep vaccines within the recommended temperature range from manufacture to administration. Key aspects of cold chain discussed include various equipment used like walk-in cold rooms, deep freezers, ice-lined refrigerators, cold boxes, vaccine carriers and ice packs. The document outlines the temperature requirements for different vaccines and nurses' responsibility in maintaining the cold chain to ensure vaccine potency.
Effective Diet plan for diabetic patientMedisys Kart
Diet Plays major role in diabetes. If you are not taking your diet properly and eating foods that should be avoided then it can be dangerous. So we are sharing some of the diet tips for diabetic patient in ppt so that you can eat right food and get rid of diabetes.
HYPERTENSION IN COMMUNITY HEALTH NURSINGHarsh Rastogi
Hypertension is a common cardiovascular disorder that poses a major public health challenge. It is defined as elevated blood pressure and is a major risk factor for cardiovascular mortality. It is classified as primary or secondary, with primary hypertension accounting for 90% of cases and having generally unknown causes. Risk factors include age, genetics, obesity, diet high in salt and saturated fat, physical inactivity, and stress. Prevention strategies focus on lifestyle modifications like healthy diet, exercise, weight control, limiting alcohol and reducing sodium intake.
Cold chain refers to the process of maintaining optimal temperature conditions for vaccines from manufacturer to administration. It involves receipt, storage, and delivery of vaccines using various equipment like cold boxes, vaccine carriers, refrigerators, and freezers. Temperature must be monitored daily and vaccines discarded if exposed to unsuitable temperatures as this impacts potency. Proper estimation, documentation, and emergency procedures are important for ensuring vaccine quality during transportation and storage in the cold chain.
KIDNEY AND DIET
KIDNEY ARE THE IMPORTANT ORGANS OF THE BODY.
THESE HELP TO FLUSH OUT THE HARMFUL PRODUCTS FROM THE BODY.
THESE MAKE A VERY IMPORTANT PART OF URINARY TRACT SYSTEM .
It is advise that the patient suffering from kidney failure,must minimize the consumption of food items,which are rich in potassium, sodium and proteins.
WWW.KARMAAYURVEDA.COM
.
Instead,they must consume food products which are rich in anti-oxidants,vitamins,phytochemicals and flavanoids.
WWW.KARMAAYURVEDA.COM
DIET IN KIDNEY FALIOUR
The patients with kidney failure must pursue a strict diet regime to lead a healthy life and avoid further complications. Following is a diet chart, which the patients suffering from kidney failure must follow.
FOOD PRODUCTS TO BE AVOID
PULSES(rich in proteins and potassium content)
×soybeans
× winged beans
× black beans
× white beans
FOOD PRODUCTS TO BE AVOID
WWW.KARMAAYURVEDA.COM
FOOD PRODUCTS TO BE AVOID
WWW.KARMAAYURVEDA.COM
FOOD PRODUCTS TO BE AVOID
WWW.KARMAAYURVEDA.COM
FOOD PRODUCTS TO BE AVOID
Generally all the spices contain approximately more than 50 percent of potassium in them.
So , it is recommended to avoid much consumption of spices of any from.
Use salt in very less quality.
Don’t use tablets salts.
OTHER FOODS PRODUCTS
FOOD PRODUCTS TO BE COMSUMED
WWW.KARMAAYURVEDA.COM
FOOD PRODUCTS TO BE COMSUMED
FOOD PRODUCTS TO BE CONSUMED
VEGETABLES
FOOD PRODUCTS TO BE CONSUMED
CARDAMOM
BLACKPEPPER
SUNFLOWER OIL
OLIVE OIL
CORIANDER
MINT
BAYLEAVES
FRNUGREEK/METHI
FOOD PRODUCTS TO BE CONSUMED
MILK-LOW FAT
MUNGDAL KHICHADI
WHEAT
RICE
THE PATIENT SUFFERING FROM KIDNEY FALIOUR ARE STRICTLY ADVISE TO FOLLO THE ABOVE DIET CHART PROPERLY.
THIS DIET CHART IS PREPARED BASED ON THE NUTRITIONAL CONTENT CARBOHYDRATES , PROTEINS ,FATS AND VITAMINS
The document discusses key aspects of the Children Act 1989 in the UK. It establishes that a child's welfare is the paramount consideration in legal decisions. It defines parental responsibility and outlines the duties of local authorities to investigate child welfare concerns and protect children, including through care and supervision orders if needed. The Act aims to reform laws around protecting children's rights and well-being.
This document discusses the importance of nutrition education programs in developing countries. It notes that lack of awareness about dietary requirements and food nutrition is a major cause of malnutrition. Nutrition education should be practical and tailored to the local socioeconomic conditions, food habits, and available food resources. The goals of nutrition education programs are to increase awareness, enhance motivation, facilitate actions to improve nutrition, and improve environmental support. Common methods of nutrition education discussed include lectures, demonstrations, workshops, films, posters, printed materials, radio, and television.
This document outlines important nursing diagnoses for several common conditions:
1. For fever, key diagnoses include hyperthermia, risk for dehydration, knowledge deficit about the disease, and anxiety. Sleep may also be disturbed.
2. For diabetes, important diagnoses are knowledge deficit about managing the disease, risk for unstable blood sugar, risk for infection, and activity intolerance from hypo/hyperglycemia. Anxiety is also common.
3. For hypertension, risk for decreased cardiac output, acute headache pain, ineffective tissue perfusion, knowledge deficit, and anxiety are key.
4. For appendectomy, acute pain, risk for fluid deficit, risk for infection, knowledge deficit, and anxiety are the major nursing
A crash cart or code cart (crash trolley in UK medical jargon) or "MAX cart" is a set of trays/drawers/shelves on wheels used in hospitals for transportation and dispensing of emergency medication/equipment at the site of medical/surgical emergency for life support protocols to potentially save someone's life.
This document provides information on newborn characteristics, assessment, and care. It describes the typical physical attributes of newborns such as weight, length, skin appearance. Vital signs at birth and how they adjust in the first hours are outlined. The document emphasizes the importance of thoroughly assessing the newborn to identify any issues and ensure healthy transition after birth. Key parts of physical exam and Apgar scoring are explained. Guidelines for routine newborn care like cleaning, feeding, and rooming-in with mothers are provided.
This document provides information on nursing care for patients undergoing dialysis. It discusses protocols for hemodialysis and peritoneal dialysis. Hemodialysis requires vascular access, a dialysis machine, dialysate solution, and takes place over 12 hours per week divided into 3 sessions. Complications can include infection, blood clotting, and hypotension. Nursing considerations include medication management, access site care, monitoring for complications, and providing education and psychological support. Peritoneal dialysis can cause discomfort and complications like peritonitis. Special care is needed for hospitalized dialysis patients to prevent infections and control electrolyte levels.
An enema is the introduction of fluid into the lower bowel through the rectum to cleanse or administer medication. There are several types of enemas that serve different purposes such as treating constipation, softening stool, administering medication, or nourishing patients. The document outlines various enema solutions used, including soap and water, oil, glycerin, and medications. It also describes procedures for administering different types of enemas and important safety considerations.
The document discusses the benefits of exercise for mental health. Regular physical activity can help reduce anxiety and depression and improve mood and cognitive function. Exercise causes chemical changes in the brain that may help alleviate symptoms of mental illness and boost overall mental well-being.
Multinationals are organizations headquartered in one country that operate in two or more other countries. They have advantages like taking advantage of grants, increasing market share, and cutting costs through cheaper labor. However, they also have disadvantages like paying tax in multiple countries and ensuring they observe local traditions. For the countries they operate in, multinationals can create jobs and introduce new technologies, but can also have too much influence and send profits back to their home country. The document provides an outline for a task analyzing a specific multinational company in terms of its profile, reasons for being multinational, and advantages/disadvantages for the company and countries it operates in.
The document outlines a 5 day travel itinerary through Argentina:
Day 1 in Buenos Aires, staying in a 5 star hotel with meals included. Day 2 in the litoral region visiting Iguazu Falls. Day 3 in Cordoba for adventure activities like trekking. Day 4 in the north region for horseback riding and village tours. Day 5 in the Cuyo region among the Andes mountains for winter sports, hang gliding, and canoeing. The trip offers a variety of landscapes and cultural experiences throughout Argentina.
Обсуждаем мягкие (ПО) тенденции на секции №9 на конгрессе "Подмосковные вечер...Michael Kozloff
В секции №9 мы намерены обсудить мягкие ИТ-тенденции (корпоративное ПО). Возьмем за основу прогноз Gartner и сравним его с мнением наших экспертов.
Главная тенденция в ИТ сегодня состоит в том, что ПО начинает править миром . Об этом, в частности, свидетельствуют недавние события с HP и Google.
Самые быстрорастущие компании в мире строят свой бизнес целиком или во многом на ПО. Проблемы с ПО приводят к остановке ядерной программы Ирана. Фотоиндустрия за какие-то 10-12 лет стала полностью зависимой от ПО...
Мы опросим наших экспертов и построим свой собственный сводный рейтинг Топ 5 тенденций в мире российского корпоративного ПО, сравнив его с глобальным прогнозом Gartner
This document is a newsletter from UNITE, a support group for parents who have experienced miscarriage, stillbirth, or infant death. It contains several poems submitted by members that express their grief over the loss of their children. It also includes announcements about upcoming support group meetings and information on how to sponsor an issue of the newsletter. The overall purpose is to provide a space for bereaved parents to share their experiences and find community.
1) Sinopa gets separated from the others when escaping through a portal in the bookshelves. She ends up in the same room as her cousin Siksika, who has been tortured by their captor Suni.
2) The others escape to a library through the portal bookshelves. They begin searching books for clues while worried about Sinopa and Siksika.
3) Sinopa remains invisible in the corner as Suni continues torturing Siksika. She telepathically updates her twin sister Rozene on the situation, waiting for the right moment to act.
The document discusses developing a marketing analytics platform to optimize marketing campaigns and measure sales response. It involves:
1) Collecting various data sources like call volumes, website behavior, surveys to understand customers.
2) Identifying key performance indicators like awareness, consideration, intent, loyalty that have predictive linkage to sales.
3) Building analytics solutions like dashboards and reports to convert data into actionable insights for marketing programs and sales.
4) Taking an integrated and analytical approach using various tools like regression, genetic programming, structural equation modeling, and simulators to examine relationships and identify patterns in the data.
The document summarizes the history and development of the Minuteman Bikeway in Massachusetts. It began as the Lexington and West Cambridge Railroad in the late 19th century, providing passenger and freight service until declining in the mid-20th century. In the 1980s, Tom Fortmann and Alan McClennen advocated for converting the abandoned rail line into a bike path. The path opened in 1993 after $3.2 million and 15 years of work. It is now an 11.2 mile multi-use path from Bedford to Cambridge that sees over 2 million users annually for activities like biking, walking, running, and cross-country skiing.
This document provides details about a collection of sporting cartoons by British humorist Mark H., including titles, images, and descriptions. There are over 20 cartoons available, focusing on humorous takes on equestrian activities like horse racing, fox hunting, and eventing. Each cartoon captures Mark H.'s unique sense of humor related to the sporting world through witty observations and scenarios. The cartoons are available for purchase from the Chisholm Gallery for $190 each, not including shipping or taxes.
This document is a member directory from the website Patexia.com from November 2014. It lists the first 20 members out of a total of 5,850 members. The members are categorized by their profession as legal, business, or science and technology. The directory provides the members' names and indicates whether they are new members. It also contains navigation buttons to browse through additional pages of members.
Juan has experience with various outdoor activities like shooting guns in the country and fishing but has yet to cook chicken. He has also attended a recital and gone to a race.
María Julia is on holiday in Alta Gracia, a small, beautiful province located a few kilometres from Córdoba city in Argentina. She is staying at the great Howard Johnson Sierras Hotel and has included two photos - the first of the Old Jesuit Residence, now a museum where Jesuit fathers once lived, and the second of the Clock Tower built in 1938 that represents the passing of time in the city's history.
Camila Caunedo was born in Santa Rosa, Argentina. She shares about her family, which consists of her mother, brother, and grandparents who she visits each summer in Mar del Plata. She enjoys swimming, playing with her grandfather, and cooking with her grandmother during these visits. Camila also discusses her friends Julieta, Victoria, Camila, and another Julieta, describing their personalities and activities they enjoy together like playing hockey.
ComunicaCity è una mobile app per smartphone e tablet che permette agli enti pubblici e alle pubbliche amministrazioni (comuni, provincie, regioni, forze dell’ordine, ministeri, comunità) di inviare al cittadino e alle imprese news, segnalazioni, allerte, aggiornamenti, comunicati, il tutto in tempo reale e in maniera semplice e veloce. All’interno dell’app ci sono inoltre sezioni ad hoc per la raccolta differenziata, per il turismo, info utili, ecc. L’aspetto più innovativo e unico di ComunicaCity è la possibilità, per l’utente, di seguire tutti gli enti che desidera, in una sola app. La ricchezza di funzionalità e informazioni di ComunicaCity la rende veramente appetibile per tutti i cittadini e le imprese.
Il servizio prevede un canone annuale per gli enti variabile in base al numero di abitant, mentre è gratuita per gli utenti finali.
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Safe Preparation, Storage And Handling Of Powdered Infant Formula Guidelines
1. Safe preparation,
storage and handling
of powdered infant
formula
Guidelines
World Health Organization
in collaboration with
Food and Agriculture Organization
of the United Nations
3. Safe preparation, storage and handling of powdered infant formula - Guidelines
Contents
Executive Summary ii
Acknowledgements ii
Part 1: Introduction
1.1 Background 2
1.2 Illness associated with PIF 2
1.2.1 E. sakazakii 3
1.2.2 Salmonella 3
1.3 Populations at greatest risk of infection 4
1.4 Contamination of PIF 4
1.5 Breastfeeding recommendation 5
1.6 Purpose 5
1.7 Scope 5
1.8 Assumptions behind the recommendations 6
1.9 Training 6
Part 2: In Care Settings
2.1 Recommendations 8
2.1.1 Use of infant formula 8
2.1.2 General requirements 8
2.1.4 Preparing a feed using PIF 9
2.1.5 Preparing feeds in advance for later use 10
2.1.6 Re-warming stored feeds 10
2.1.7 Transporting feeds 10
2.1.8 Holding and feeding times 11
2.2 Rationale behind recommendations 11
2.2.1 Choice of infant formula 11
2.2.2 General requirements 11
2.2.3 Good hygienic practice 12
2.2.4 Cleaning and sterilizing feeding and preparation equipment 12
2.2.5 Temperature of reconstitution water 12
2.2.6 Volume of container for preparing batches 12
2.2.7 Holding and feeding times 13
2.2.8 Labelling of feed 13
2.2.9 Storage of prepared feeds 13
2.2.10 Re-warming stored feeds 14
2.2.11 Transporting prepared feeds 14
Part 3: In The Home
3.1 Recommendations 16
3.1.1 Cleaning and sterilizing feeding and preparation equipment 16
3.1.2 Preparing a feed using powdered infant formula 17
3.1.3 Preparing feeds in advance for later use 17
3.1.4 Re-warming stored feeds 18
3.1.5 Transporting feeds 18
3.2 Rationale behind recommendations 18
3.2.1 Good hygienic practice 18
3.2.2 Cleaning and sterilizing feeding and preparation equipment 18
3.2.3 Temperature of water for reconstitution 19
3.2.4 Storage of prepared feeds 19
3.2.5 Re-warming and use of stored feeds 19
3.2.6 Transporting feeds 19
3.2.7 Holding and feeding times 20
Appendices
Appendix 1 22
Appendix 2 23
Appendix 3 24
References Cited in the Text 25
4. Safe preparation, storage and handling of powdered infant formula - Guidelines
Executive Summary
Powdered infant formula (PIF) has been associated with serious illness and death in infants due to infections
with Enterobacter sakazakii. During production, PIF can become contaminated with harmful bacteria, such as
Enterobacter sakazakii and Salmonella enterica. This is because, using current manufacturing technology, it is
not feasible to produce sterile PIF. During the preparation of PIF, inappropriate handling practices can exacerbate
the problem.
Recognizing the need to address such hazards in PIF, Codex Alimentarius decided to revise the Recommended
International Code of Hygienic Practice for Foods for Infants and Children. In doing so it requested specific
scientific advice from the Food and Agriculture Organization of the United Nations (FAO) and the World Health
Organization (WHO). FAO and WHO have provided this advice in the reports of two expert meetings held in
2004 and 2006 on Enterobacter sakazakii and other microorganisms in powdered infant formula (PIF). Part of
this advice included recommendation to develop guidelines for the preparation of PIF.
The World Health Assembly (WHA) of WHO requested in 2005 the Organization to develop such guidelines on
the safe preparation, handling and storage of PIF in order to minimize the risk to infants.
The FAO/WHO advice on E. sakazakii in PIF includes a quantitative microbiological risk assessment of E. sakazakii
in PIF. One of the aspects of the risk assessment was to determine relative risk reduction associated with
different preparation, storage and handling scenarios. The recommendations made in the present guideline
document are largely based on the findings of the quantitative risk assessment. No risk assessment was carried
out for Salmonella, but the group reported that the basic risk control principles for E. sakazakii would also hold
true for S. enterica.
In general, sterile liquid infant formula is recommended for infants at the highest risk of infection. Where sterile
liquid infant formula is not available, preparation of PIF with water at a temperature of no less than 70 °C
dramatically reduces the risk. Minimizing the time from preparation to consumption also reduces the risk, as
does storage of prepared feed at temperatures no higher than 5 °C.
Users of PIF are made aware that powdered infant formula is not a sterile product and may be contaminated
with pathogens that can cause serious illness. Correct preparation and handling of PIF reduces the risk of illness.
The present guidelines are presented in two parts. One part provides guidance for the preparation of PIF in care
settings where professional care providers are involved in the preparation of large quantities of PIF for a large
number of infants. The second part provides guidance for the preparation of PIF in a home environment, aimed
at parents and those involved in the care of infants in the home environment.
The document provides specific guidance on the most appropriate practices in the different steps during
the preparation of PIF in these two types of settings. Cleaning and sterilization of feeding and preparation
equipment is an important prerequisite to the safe preparation of PIF. The specific guidance focuses on
the most important parameters during preparation such as the temperature of reconstitution, the cooling,
holding and feeding times, as well as the storage and transportation of prepared PIF. The rationale behind the
recommendations is provided in both sets of guidance.
Acknowledgements
The World Health Organization would like to express its appreciation to all those who contributed to the
preparation of these guidelines. Special appreciation is extended to the Food Safety Authority of Ireland and
particularly to Judith O'Connor and Alan Reilly for their time, efforts and expertise provided in the elaboration
of these guidelines. Appreciation is also extended to the many people in more than 20 countries as well
as several stakeholder associations who have provided their comments and suggestion following a call for
comments issued through the International Food Safety Authorities Network (INFOSAN).
The preparation of these Guidelines was coordinated by WHO in collaboration with FAO, with contributions
from Peter Karim Ben Embarek, Jaap Jansen, Margaret Miller, Jenny Bishop, Janis Bernat, Françoise Fontannaz,
Jenny Murcott and Jørgen Schlundt in WHO, with Sarah Cahill and Maria de Lourdes Costarrica in FAO.
5. Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 1: Introduction
1.1 Background 2
1.2 Illness associated with PIF 2
1.2.1 E. sakazakii 3
1.2.2 Salmonella 3
1.3 Populations at greatest risk of infection 4
1.4 Contamination of PIF 4
1.5 Breastfeeding recommendation 5
1.6 Purpose 5
1.7 Scope 5
1.8 Assumptions behind the recommendations 6
1.9 Training 6
6. Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 1: Introduction
1.1 Background
In 2004, the Food and Agriculture Organization of the United Nations (FAO) and the World Health
Organization (WHO) met jointly in Geneva to convene an expert meeting on Enterobacter sakazakii and other
microorganisms in powdered infant formula (PIF). This meeting was organized in response to a request from the
Codex Committee on Food Hygiene (CCFH) for input into the revision of the Recommended International Code
of Hygienic Practice for Foods for Infants and Children (CAC, 1979).
Based on the literature reviewed, the expert meeting concluded that E. sakazakii and Salmonella enterica
were the organisms of most concern in PIF. The expert meeting conducted a preliminary risk assessment for
E. sakazakii, which established that the inclusion of a pathogenic lethal step at the point of preparation (e.g.
reconstituting PIF with water at no less than 70 °C) and a decrease in the holding time and feeding times would
effectively reduce risk. Based on this preliminary risk assessment, the expert group made recommendations
to FAO, WHO, Codex, member countries, nongovernmental organizations and the scientific community for
minimizing the risk (Appendix 1). One recommendation was that “Guidelines should be developed for the
preparation, use and handling of infant formula to minimize the risk”.
The World Health Assembly (WHA) of WHO, in 2005, in its Resolution WHA 58.32 (WHA, 2005), requested
the Organization to develop such guidelines on the safe preparation, handling and storage of PIF in order to
minimize the risk to infants.
A second meeting of the FAO/WHO expert group was convened in January 2006, to address additional requests
from the CCFH taking into consideration new scientific data (on E. sakazakii and S. enterica), and to apply a
quantitative microbiological risk assessment model for E. sakazakii in PIF. This model had been developed since
the first meeting in 2004. One of the aspects of the risk assessment was to determine relative risk reduction
associated with different preparation, storage and handling scenarios. The recommendations made in this
guidance document are largely based on the findings of the quantitative risk assessment.
No risk assessment was carried out for Salmonella, but the group reported that the basic risk control principles
for E. sakazakii would also hold true for S. enterica. However, the specific risk reductions achieved would vary
to some degree, based on the mode and sources of Salmonella contamination and its growth and survival
characteristics.
A first draft of the present guidelines was developed based on existing national guidelines and on the
outcome of the risk assessment. Extensive consultation on the draft guidelines was undertaken through the
International Food Safety Authorities Network (INFOSAN). Comments received from more than 20 INFOSAN
Member countries and International Organizations representing stakeholders were considered and appropriate
modifications were made to the draft guidelines.
1.2 Illness associated with PIF
PIF is not a sterile product, even if it has been manufactured to meet current hygiene standards. This means
that it may occasionally contain pathogens that can cause serious illness.
The FAO/WHO expert working groups (2004 2006) concluded that E. sakazakii and Salmonella enterica are
the pathogens of most concern in PIF. Severe illness and sometimes death in infants has been attributed to PIF
that has been contaminated with E. sakazakii or Salmonella, at either the manufacturing or preparation stage.
Because the manufacture of commercially sterile PIF is not feasible using current processing technology, there
is a potential risk of infection to infants through consumption of PIF. This risk is increased when prepared feeds
are handled or stored incorrectly.
7. Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 1: Introduction (continued)
Extrinsic contamination of PIF is possible from the person preparing the formula and the environment the
formula is prepared in. Specific food hygiene control measures have been included in these guidelines to help
address these issues.
1.2.1 E. sakazakii
E. sakazakii was first implicated in a case of neonatal meningitis in 1958, and since then there have been
around 70 reported cases of E. sakazakii infection (Drudy et al., 2006). However, it is likely that E. sakazakii is
significantly under-reported in all countries. Although E. sakazakii can cause illness in all age groups, infants are
believed to be at greatest risk of infection.
In 2004, PIF was microbiologically linked to two E. sakazakii outbreaks, in New Zealand and in France (FAO/
WHO, 2006). The French outbreak involved nine cases, and resulted in the death of two infants. While eight of
the cases were in premature infants of low birth weight (2 kg), one case was in an infant born at 37 weeks
and weighing 3.25 kg. The outbreak involved five hospitals, and a review of practices in the hospitals revealed
that one hospital was not following recommended procedures for the preparation, handling and storage of
feeding bottles, and four were storing reconstituted formula for 24 hours in domestic-type refrigerators, with
no temperature control or traceability.
Limited information was available on the numbers of E. sakazakii organisms that ill patients were exposed to
in any of the various outbreaks. It is therefore not possible to develop a dose-response curve for E. sakazakii
(FAO/WHO, 2006). However, it is possible that a small number of cells present in PIF could cause illness. This
risk increases rapidly when bacteria in the reconstituted formula are allowed to multiply, such as by holding at
inappropriate temperatures for an extended period.
In the United States of America, an incidence rate of 1 per 100 000 infants for E. sakazakii infection has been
reported. This incidence rate increases to 9.4 per 100 000 in infants of very low birth weight, i.e. 1.5 kg
(FAO/WHO, 2006).
1.2.2 Salmonella
At least six PIF-associated salmonellosis outbreaks have been described since 1995, across Canada, France,
Korea, Spain, UK and USA (FAO/WHO, 2006). The most recent was an outbreak of S. agona that occurred in
France in 2005. This outbreak involved 141 infants, all of whom were under 12 months of age.
Although the infectious dose for infants, or specific groups of infants, is not known, information from outbreak
investigations indicates that at least some Salmonella serotypes have potential to cause illness at very low doses.
This may be a specific concern for infants, particularly those in the higher susceptibility category (premature;
low birth weight; immunocompromised).
The United States of America reported a salmonellosis incidence rate of 139.4 cases per 100 000 infants in
2002. The incidence rate for infants was more than eight times higher than that of the general population
(16.2 per 100 000) (CDC, 2002).
8. Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 1: Introduction (continued)
1.3 Populations at greatest risk of infection
Although E. sakazakii can cause illness in all age groups, infants (children 1 year) are at most risk with
neonates and infants under two months at greatest risk. The groups of infants at greatest risk includes in
particular pre-term infants, low-birth-weight (2.5 kg) infants or immunocompromised infants. However, infants
who are compromised for any other reason may also be at greater risk of E. sakazakii infection. Infants of
HIV-positive mothers are also at risk because they may be immunocompromised and may specifically require
PIF (FAO/WHO, 2004). There appear to be two distinct infant risk groups for E. sakazakii infection: premature
infants who develop bacteraemia after one month of age, and term infants who develop meningitis during the
neonatal period. Therefore, the FAO/WHO expert working group (2006) concluded that while infants appear to
be the group at particular risk, neonates and also those less than two months of age are at greatest risk (FAO/
WHO, 2006).
It is very important to note that, although high-risk groups of infants have been identified, E. sakazakii infection
has occurred in previously healthy infants outside the neonatal period (Gurtler, Kornacki and Beuchat, 2005).
Furthermore, infections have occurred in both hospital and outpatient settings. For this reason, educational
messages on the safe preparation and handling of PIF are required for health-care workers, parents and other
infant carers.
In the case of salmonellosis, infants are more likely than the general population to experience severe illness or
death. Immunocompromised infants are particularly vulnerable. While infants who are breastfed are 50% less
likely to contract salmonellosis, a few reports have described the transmission of Salmonella via expressed breast
milk (FAO/WHO, 2006).
1.4 Contamination of PIF
Current manufacturing processes cannot achieve the production of sterile PIF. Contamination of PIF with E.
sakazakii and Salmonella can occur intrinsically, or from extrinsic sources. Intrinsic contamination occurs at some
stage during its manufacture (e.g. from the manufacturing environment, or from raw ingredients).
Recent data point to differences in the microbial ecology of Salmonella spp. and E. sakazakii. E. sakazakii is
more commonly found in the manufacturing environment than Salmonella. Surveys have identified E. sakazakii
in 3-14% of PIF samples (FAO/WHO, 2006), but the levels of contamination reported have been low: 0.36-
66.0 cfu/100 g (Forsythe, 2005). In contrast, Salmonella is rarely found in PIF. In one survey, no Salmonella
were found in samples from 141 different formulas (Muytjens, Roelofs-Willemse and Jasper, 1988). The current
Codex specification for Salmonella is the absence of organisms in 60 samples of 25 g each. Specific criteria for
E. sakazakii, however, are not included, but come under the general category of coliforms (CAC, 1979). The
standard requires a minimum of 4 to 5 samples with 3 coliforms/g and a maximum of 1 in 5 control samples
with levels 3 but 20 coliforms/g. This is currently under review in the Codex Committee on Food Hygiene.
Extrinsic contamination can occur when contaminated utensils (e.g. spoons, blenders, bottles, teats) are used
for preparing or feeding PIF, or contamination may occur from the preparation environment.
While E. sakazakii and Salmonella do not grow in dry PIF, they can survive for long periods. E. sakazakii has
been shown to survive up to and beyond one year in dry PIF (Forsythe, 2005). Reconstituted PIF, however,
provides an ideal environment for the growth of pathogens. Storage of reconstituted PIF at temperatures not
more than 5 °C will prevent the growth of Salmonella and E. sakazakii. However, held above this temperature
(e.g. at room temperature), there is the potential for rapid growth of E. sakazakii or Salmonella, particularly if
held for extended periods.
9. Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 1: Introduction (continued)
1.5 Breastfeeding recommendation
WHO recommends that infants should be exclusively breastfed for the first six months of life to achieve optimal
growth, development and health. Thereafter, to meet their evolving nutritional requirements, infants should
receive nutritionally adequate and safe complementary foods while breastfeeding continues for up to two years
of age or beyond (WHO/UNICEF, 2003).
It is important to support breastfeeding and promote its benefits to infants and young children. There are,
however, instances where breast milk is not available, where the mother is unable to breastfeed, where they
have made an informed decision not to breastfeed, or where breastfeeding is not appropriate, e.g. where the
mother is taking medication that is contraindicated for breastfeeding or the mother is HIV-positive1. Similarly,
some very low-birth-weight babies may not be able to breastfeed directly, and in some cases expressed breast
milk may not be available at all or available in insufficient quantities.
Infants who are not breastfed require a suitable breast-milk substitute, for example, an infant formula prepared
in accordance with the present guidelines.
1.6 Purpose
The purpose of this document is to deliver recommendations on the safe preparation, storage and handling of
PIF, in order to reduce the risk of infection from E. sakazakii and S. enterica. In principle, infant formula should
be used only when medically indicated2 in accordance with the 10 Steps of the WHO/UNICEF Baby-Friendly
Hospital Initiative (BFHI) (Appendix 2).
These present guidelines are considered to be a generic document that will provide guidance and support for
countries and governments. When adapted at the country level, conditions (i.e. climatic and socioeconomic
differences, etc.) within the country should be reflected. Individual countries should outline minimum training
requirements for parents, caregivers, and staff in hospitals and day-care centres.
Specific details of illness associated with PIF, sources of contamination, characteristics of E. sakazakii and
Salmonella have been covered in full in FAO/WHO reports (FAO/WHO, 2004, 2006) and are therefore only
summarized in this document (Sections 1.2-1.4).
1.7 Scope
These guidelines provide recommendations for the preparation of PIF in care settings and in the home.
The guidelines in this document apply only to the preparation of PIF for infants no more than 12 months (as
defined in Codex ALINORM 07/30/26) (CAC, 2007). Follow-up formula (as defined in Codex Standard 156-
1987) (CAC, 1987) and formula for special medical purposes intended for infants (as defined in Codex Alinorm
07/30/26, Appendix II) (CAC, 2007) are considered outside the scope of this document. However, it should be
noted that, in the absence of other guidance, the preparation of these formulas should follow that of PIF for
infants no more than 12 months.
1 Exclusive breastfeeding is recommended for HIV-infected women for the first 6 months of life unless replacement feeding is acceptable,
feasible, affordable, sustainable and safe for them and their infants before that time. When replacement feeding is acceptable, feasible,
affordable, sustainable and safe, avoidance of all breastfeeding by HIV-infected women is recommended. http://www.who.int/child-adolescent-
health/publications/NUTRITION/consensus_statement.htm
2 The marketing of PIF should meet the requirements of the WHO/UNICEF International Code of Marketing of Breast-Milk Substitutes (WHO,
1981) and all relevant resolutions of the World Health Assembly. The aim of the Code is to contribute to the provision of safe and adequate
nutrition for infants, by the protection and promotion of breastfeeding, and by ensuring the proper use of breast-milk substitutes, when these
are necessary, on the basis of adequate information and through appropriate marketing and distribution.
10. Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 1: Introduction (continued)
1.8 Assumptions behind the recommendations
As PIF may contain pathogens, one of the recommendations from the joint FAO/WHO meeting in Geneva,
2004 (FAO/WHO, 2004), and the basis for the WHA resolution request, was that guidance on the preparation,
use and handling of PIF is required because many people who prepare PIF (both general public and health
professionals) are not aware of the risks associated with this product, nor are they familiar with best practice for
its reconstitution.
The recommendations are largely based on the findings of a FAO/WHO risk assessment on E. sakazakii in PIF,
conducted in January 2006 (FAO/WHO, 2006). The quantitative risk assessment model that was developed
calculates the increase or decrease in the relative risk associated with different feeding practices, compared to a
baseline scenario.
These recommendations apply to persons preparing and handling PIF in care settings and in the home. In
general, a dramatic reduction in risk is achieved when PIF is reconstituted with water that is no less than 70 °C.
Risk is controlled by minimizing the time from preparation to consumption.
These recommendations are made under the assumption that the person preparing PIF has access to safe
water; soap; a clean preparation environment; boiling water; and refrigeration. Where water quality is poor,
boiling, chlorination and filtration are important means to inactivate microbial pathogens and make the water
safe. To disinfect water: bring to a rolling boil; add 3-5 drops of bleach to 1 litre of water; or physically remove
pathogens with the appropriate filter.
In certain circumstances (e.g. developing countries, or in the case of emergencies) one or more of these
resources may be unavailable. In these cases, the simplest, most effective measures for reducing the risk of
illness from the use of PIF are:
• Reconstitution with boiling water and consumption as soon as the formula is cool.
• Where boiling water is not available, reconstitute PIF with safe water at room temperature, and consume
immediately.
1.9 Training
All persons preparing feeds from PIF should be informed about the risk associated with PIF and trained in or
informed about its safe preparation according to these guidelines. As these guidelines recommend the use
of very hot water, additional information or training, or both, should be provided on the safe handling of
hot water. The present guideline document is divided into two main sections. Part 2 provides guidance for
the preparation of PIF in care settings, and part 3 provides guidance for the preparation of PIF in the home
environment. Both sections include a rational for the recommendations provided. Both environments have many
similarities and therefore part of the guidance and rational is very similar between the two sections. Depending
on the targeted end user, specific guidance material using either part 2 or part 3 should be developed.
11. Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 2: In Care Settings
2.1 Recommendations 8
2.1.1 Use of infant formula 8
2.1.2 General requirements 8
2.1.3 Cleaning and sterilization of feeding and preparation equipment 8
2.1.4 Preparing a feed using PIF 9
2.1.5 Preparing feeds in advance for later use 10
2.1.6 Re-warming stored feeds 10
2.1.7 Transporting feeds 10
2.1.8 Holding and feeding times 11
2.2 Rationale behind recommendations 11
2.2.1 Choice of infant formula 11
2.2.2 General requirements 11
2.2.3 Good hygienic practice 12
2.2.4 Cleaning and sterilizing feeding and preparation equipment 12
2.2.5 Temperature of reconstitution water 12
2.2.6 Volume of container for preparing batches 12
2.2.7 Holding and feeding times 13
2.2.8 Labelling of feed 13
2.2.9 Storage of prepared feeds 13
2.2.10 Re-warming stored feeds 14
2.2.11 Transporting prepared feeds 14
12. Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 2: In Care Settings
PIF is not a sterile product and may be contaminated with pathogens that can cause serious illness. Correct
preparation and handling reduces the risk of illness.
Where available, commercially sterile ready-to-feed liquid infant formula should be used for infants at greatest
risk. Sterile liquid infant formula does not contain pathogenic microorganisms and so does not pose a risk of
infection. However, its use may not always be an option, and the use of PIF may be required.
PIF is not a sterile product and may contain harmful bacteria. Reconstituted PIF provides an ideal environment
for the growth of these pathogens. Even if present in powdered formula at very low levels, inappropriate
preparation and handling of reconstituted PIF provides ideal conditions for these pathogens to multiply, which
greatly increases the risk of infection. However, the risk of illness can be reduced if PIF is prepared safely and
handled correctly.
Care settings include hospitals and day-care centres such as crèches. Hospitals, especially intensive care units,
that provide care for vulnerable infants are at greatest risk of E. sakazakii infection, as discussed earlier.
Hospitals and day-care centres may also be required to prepare large batches of PIF in advance, for later use.
This practice may increase the risk of E. sakazakii infection if not carried out correctly.
Because PIF is not a sterile product, there is an innate risk of infection with bacteria such as E. sakazakii. The
recommendations below outline best practice for the preparation, storage and handling of PIF in care settings in
order to reduce the risk of infection with E. sakazakii. These recommendations are also appropriate for reducing
the risk of infection with Salmonella.
2.1 Recommendations
2.1.1 Use of infant formula
1. Infant formula should be selected based on the medical needs of the infant.
2. Where available, use commercially sterile liquid infant formula for infants at greatest risk.
2.1.2 General requirements
1. Each institution should establish written guidelines for the preparation and handling of PIF.
2. The implementation of guidelines should be monitored.
3. Personnel preparing PIF should be fully trained according to the guidelines and trained in hygiene
requirements for food preparation.
4. There should be full traceability of PIF prepared in care settings.
5. There should be a clean dedicated area for preparation and storage of PIF. Additional guidance on the layout
of the preparation room should be communicated at country level.
2.1.3 Cleaning and sterilization of feeding and preparation equipment
It is very important that all equipment used for feeding infants and for preparing feeds has been thoroughly
cleaned and sterilized before use.
1. Hands should always be washed thoroughly with soap and water before cleaning and sterilizing feeding
and preparation equipment (as described below). In care settings, a dedicated hand-washing sink is
recommended.
2. Cleaning: wash feeding and preparation equipment (e.g. cups, bottles, teats and spoons) thoroughly in hot
soapy water. Where feeding bottles are used, clean bottle and teat brushes should be used to scrub inside
and outside of bottles and teats to ensure that all remaining feed is removed.
13. Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 2: In Care Settings (continued)
3. After washing feeding and preparation equipment, rinse thoroughly in safe water.
4. Sterilizing: if using a commercial sterilizer, follow manufacturer's instructions. Feeding and preparation
equipment can also be sterilized by boiling:
a. fill a large pan with water and completely submerge all washed feeding and preparation equipment,
ensuring that there are no trapped air bubbles;
b. cover the pan with a lid and bring to a rolling boil, making sure the pan does not boil dry; and
c. keep the pan covered until the feeding and preparation equipment is needed.
5. Hands should be washed thoroughly with soap and water before removing feeding and preparation
equipment from a sterilizer or pan. The use of sterilized forceps for handling sterilized feeding and
preparation equipment is recommended.
6. To prevent recontamination, it is best to remove feeding and preparation equipment just before it is required
for use. If equipment is removed from the sterilizer and not used immediately, it should be covered and
stored in a clean place. Feeding bottles can be fully assembled to prevent the inside of the sterilized bottle
and the inside and outside of the teat from becoming contaminated.
2.1.4 Preparing a feed using PIF
It is best to prepare feeds fresh each time and to feed immediately. Hospitals and other care settings will be
required to prepare feeds for many infants. Ideally, each feed should be prepared in an individual feeding cup
or bottle. However, in certain circumstances, feeds are mixed in larger containers, and then transferred into
individual feeding cups or bottles. This practice poses a risk because PIF is more susceptible to contamination in
large, open containers. Also, large volumes of feed take much longer to cool, leaving the potential for growth
of harmful bacteria. The recommendations below outline the safest practice for preparing feed in individual
containers or in batches for immediate consumption:
1. Clean and disinfect a surface on which to prepare the feed.
2. Wash hands with soap and water, and dry using a clean cloth or a single-use napkin.
3. Boil a sufficient volume of safe water. If using an automatic kettle, wait until the kettle switches off;
otherwise make sure that the water comes to a rolling boil. Note: bottled water is not sterile and must be
boiled before use. Microwave ovens should never be used in the preparation of PIF as uneven heating may
result in 'hot spots' that can scald the infant's mouth.
4. Taking care to avoid scalds, pour the appropriate amount of boiled water, which has been allowed to cool
slightly, but not below 70 °C, into a cleaned and sterilized feeding cup or bottle. The temperature of the
water should be checked using a sterile thermometer.
a. If making a batch in a larger container: the container should have been cleaned and sterilized. It should
be no larger than 1 litre, be made from food-grade material and be suitable for pouring hot liquids.
5. To the water, add the exact amount of formula as instructed on the label. Adding more or less powder than
instructed could make infants ill.
a. If using feeding bottles: assemble the cleaned and sterilized parts of the bottle according to the
manufacturer's instructions. Shake or swirl gently until the contents are mixed thoroughly, taking care to
avoid scalds.
b. If using feeding cups: mix thoroughly by stirring with a cleaned and sterilized spoon, taking care to avoid
scalds.
c. If preparing a batch in a larger container: stir formula using a cleaned and sterilized spoon to ensure
even mixing. Immediately pour into individual feeding cups or bottles, taking care to avoid scalds.
14. 0 Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 2: In Care Settings (continued)
6. Cool feeds quickly to feeding temperature by holding under a running tap, or placing in a container of cold
water or iced water. Ensure that the level of the cooling water is below the top of the feeding cup or the lid
of the bottle.
7. Dry the outside of the feeding cup or bottle with a clean or disposable cloth and label with appropriate
information, such as type of formula, infant's name or ID, time and date prepared, and preparer's name.
8. Because very hot water has been used to prepare the feed, it is essential that the feeding temperature is
checked before feeding in order to avoid scalding the infant's mouth. If necessary, continue cooling as
outlined in step 6 above.
9. Discard any feed that has not been consumed within two hours.
2.1.5 Preparing feeds in advance for later use
It is best to make PIF fresh for each feed and to consume immediately, as reconstituted PIF provides ideal
conditions for the growth of harmful bacteria. For practical reasons, feeds may need to be prepared in advance.
In care or institutional settings, feeds may have to be prepared in batches and stored until required. The steps
below outline the safest practice for preparing feeds in advance and storing for later use. If refrigeration is not
available, feeds should be prepared fresh and consumed immediately. They should not be prepared in advance
for later use.
1. Follow steps 1 to 7 in Section 2.1.4. If using feeding cups, a batch of formula should be prepared in a clean,
sterilized jar or container that is no larger than 1 litre and has a lid. The prepared PIF can be refrigerated in
the lidded container and dispensed into cups as needed.
2. Place cooled feeds in a dedicated refrigerator. The temperature of the refrigerator should be no higher than
5 °C and should be monitored daily.
3. Feeds can be stored in the refrigerator for up to 24 hours.
It is not recommended that batches of formula are cooled and stored in large volumes as large volumes can
promote inadequate cooling and hence lead to the growth of harmful bacteria.
2.1.6 Re-warming stored feeds
1. Remove feeds from the refrigerator just before they are needed.
2. Re-warm for no more than 15 minutes.
3. To ensure that the feed heats evenly, periodically shake or swirl the feed in its covered container. Note:
microwave ovens should never be used for re-warming feeds as uneven heating may result in 'hot spots' that
may scald the infant's mouth.
4. Check feeding temperature in order to avoid scalding the infant's mouth.
5. Discard any re-warmed feed that has not been consumed within two hours.
2.1.7 Transporting feeds
In many care settings, feeds are prepared in a central preparation area, and transported to different wards, etc.
Transporting prepared feeds poses a risk as it increases the time from preparation to consumption, providing the
opportunity for growth of harmful bacteria. If feeds will not be consumed within two hours of preparation, they
should be refrigerated before transportation, transported under refrigerated (or cool) conditions, and reheated
at the destination. The recommendations below outline the safest practice for transporting prepared feeds:
15. Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 2: In Care Settings (continued)
1. If feed will be consumed within two hours of preparation:
a. prepare feeds as in Section 2.1.4; and
b. transport and use immediately.
2. If feed will not be consumed within two hours of preparation:
a. prepare feed and place in the refrigerator as outlined in Section 2.1.5;
b. ensure that feed is cold before transporting;
c. to transport, only remove from the refrigerator immediately before transporting;
d. transport the cold feed to its destination (for transport taking over 30 minutes, refrigeration or cool bags
are recommended); and
e. re-warm at the destination, as in Section 2.1.6; or
f. alternatively, feeds transported under cool or refrigerated conditions can be returned to a refrigerator at
the destination and used within 24 hours from preparation. Feeds that have been warmed up or feeds
that have been partially consumed must not be returned to the refrigerator and should be discarded if
not consumed within 2 hours.
2.1.8 Holding and feeding times
1. Discard any feed that has not been consumed within two hours from preparation (unless refrigerated).
2. Prepared feeds can be held in the refrigerator (≤5 °C) for up to 24 hours.
3. Discard all leftover feed.
4. Preferably, the hang-time for continuous or bolus feeds should be no more than two hours at room
temperature.
5. Continuous or bolus feeds should not be warmed during feeding.
2.2 Rationale behind recommendations
2.2.1 Choice of infant formula
Infant formula should be selected based on the medical needs of the infant.
Where feasible, sterile liquid infant formula should be used in care settings, especially when feeding high-
risk infants. These feeds do not contain harmful bacteria. Care settings, such as neonatal intensive care units,
provide care for infants at greatest risk of E. sakazakii infection, i.e. neonates and those less than two months
of age. However, sterile liquid infant formula is not always available (e.g. for infants who have special dietary
needs), and PIF might be used instead.
2.2.2 General requirements
The preparation of feeds in institutions such as hospitals should be carefully controlled. This is because large
volumes of feeds may need to be prepared, and the infants consuming feeds in these settings may be at
particular risk of infection.
In order to help control the preparation of feeds from PIF, a dedicated area for preparation and storage of feeds
should be provided to reduce the risk of cross-contamination with harmful bacteria. Each institution should
establish written guidelines for the preparation and handling of feeds prepared from PIF, the implementation
of which should be monitored. This ensures consistent and safe handling. Full training should be given to staff
preparing feeds, so that they understand the risks involved with PIF and know what steps to take to ensure
these risks are reduced or controlled.
16. Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 2: In Care Settings (continued)
2.2.3 Good hygienic practice
Poor hygiene has been reported as the probable cause of some E. sakazakii outbreaks (Forsythe, 2005). The
person preparing the feed should clean and disinfect the preparation surface and wash hands with soap and
water before preparing a feed. This is because harmful bacteria can be carried on hands and can also be
present on surfaces. Washing hands and cleaning and disinfecting surfaces reduces the risk of feeds becoming
contaminated during preparation.
Hands must also be washed after using the toilet and after diaper changing because harmful bacteria, including
E. sakazakii (Drudy et al., 2006), have been found in the urine and stools of infants. These bacteria can easily be
carried on the hands and contaminate feed during its preparation.
2.2.4 Cleaning and sterilizing feeding and preparation equipment
Outbreaks of E. sakazakii infection have been attributed to equipment used for preparing feeds (Gürtler et al.,
2005). E. sakazakii is widespread in the environment and has been shown to attach and grow (from 'Biofilms')
on surfaces commonly used in infant feeding equipment, such as latex, silicon and stainless steel. It is therefore
important that all infant feeding and preparation equipment (e.g. feeding cups, bottles, rings and teats) has
been thoroughly cleaned and sterilized before use, since the formation of biofilms on such equipment may
result in reservoirs of infection that can continually contaminate feeds (Iversen, Lane and Forsythe, 2004).
2.2.5 Temperature of reconstitution water
According to the FAO/WHO risk assessment (FAO/WHO, 2006), risk is dramatically reduced when PIF is
reconstituted with water that is no less than 70 °C, as this temperature will kill any E. sakazakii in the powder.
This level of risk reduction holds even if feeding times are extended (i.e. up to two hours), and even if ambient
room temperature reaches 35 °C. Consequently, reconstituting PIF with water no less than 70 °C dramatically
reduces the risk to all infants, even slow feeding infants and infants in warm climates where refrigeration for
the prepared formula may not be readily available (e.g. developing countries).
When PIF is prepared with water that is less than 70 °C, it does not reach a high enough temperature to
completely inactivate E. sakazakii present in the powder. This is a concern for the two following reasons: a) A
small number of cells may cause illness, therefore it is important that cells present in the PIF are destroyed; and
b) there is potential for surviving cells to multiply in the reconstituted formula. This risk is increased when the
reconstituted formula is held for extended periods above refrigeration temperature.
Concerns have been raised over the use of very hot water for reconstituting PIF, but risk of E. sakazakii is only
dramatically reduced when water at a temperature of no less than 70 °C is used (see Appendix 3). Currently,
the instructions on many PIF products lead to PIF being reconstituted with water that is around 50 °C. But,
according to the FAO/WHO risk assessment, reconstitution with 50 °C water generally results in the greatest
increase in risk, unless the reconstituted formula is consumed immediately. Under no circumstances is risk
reduced when PIF is reconstituted with 50 °C water. Manufacturer's instructions should be reviewed in the
light of the findings of the risk assessment.
2.2.6 Volume of container for preparing batches
Often in care settings, a number of feeds are prepared in a single, large container, mixed and then transferred
into bottles or feeding cups. Anecdotal evidence suggests that large volumes are prepared, and left to cool for
long periods in the preparation container (refrigerated or not).
17. Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 2: In Care Settings (continued)
Preparation in larger containers increases the risk of infection, as:
• the feed is more likely to become contaminated; and
• large volumes may take a long time to cool down, meaning that the formula remains for extended periods
at a temperature that supports the growth of harmful bacteria.
The FAO/WHO risk assessment found that the use of larger containers (25 litre) for preparing and cooling feeds
was associated with increased risk as a result of the slower cooling of formula, and therefore feeds should be
cooled in small containers where possible.
2.2.7 Holding and feeding times
According to the FAO/WHO risk assessment for E. sakazakii in PIF, increased feeding durations are generally
associated with increased risk due to possible bacterial growth. This risk is increased for warmer ambient
temperatures (30 °C and 35 °C). However, when PIF is reconstituted with ≥70 °C water, risk is dramatically
reduced, and this risk reduction remains valid for feeding times of two hours. This finding has practical
implications for the reduction of risk of E. sakazakii infection for slow-feeding infants and for infants in warm
climates where ambient room temperature may be around 35 °C.
It is recommended that formula is not held at room temperature for more than two hours, even if water at
no less than 70 °C is used to reconstitute PIF. This is because the feed may have become contaminated during
preparation, or harmful bacteria may have been introduced into the cup or feeding bottle from the infant's
mouth. Also, hot water (70 °C) may have activated bacterial spores of harmful bacteria in the formula. Holding
prepared feeds above refrigeration temperature for extended periods provides the opportunity for such bacteria
to grow.
2.2.8 Labelling of feed
Prepared feeds should be labelled with details of the infant formula, patient's name, preparer's name and
preparation time and date. As care institutions look after many infants, feeds tend to be prepared in bulk.
Adequate labelling will ensure traceability of all feeds.
2.2.9 Storage of prepared feeds
If feeds are not to be consumed within two hours after preparation, they should be quickly cooled immediately
after preparation, and stored in a refrigerator (at a maximum temperature of 5 °C). Refrigerated storage, at
temperatures lower than 5 °C, prevents or slows down growth of harmful bacteria. The FAO/WHO assessment
showed a less than 1.3-fold increase in risk when prepared feeds were refrigerated properly.
Feed stored in the refrigerator should be used within 24 hours of preparation. Even if water no less than 70 °C
was used to reconstitute PIF, spoilage bacteria may have survived that can grow at refrigeration temperatures
and can cause feeds to spoil. The quality of reconstituted PIF may also deteriorate on prolonged storage. If
there is an increased risk of microbial contamination in the preparation area or environment, then storage times
should be reduced, or feeds made fresh and consumed immediately.
The refrigerator should be capable of bringing the formula to a temperature of no more than 5 °C within 1
hour of preparation. The temperature of the refrigerator should be monitored daily. Feeds should be cooled
quickly before placing into the refrigerator as hot feeds will increase the refrigerator's temperature. Feeds can
be rapidly cooled by placing under cold running water or in a bowl of cold water.
18. Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 2: In Care Settings (continued)
2.2.10 Re-warming stored feeds
Because of the possibility of growth of harmful bacteria at temperatures above 5 °C, stored formula should
only be removed from the refrigerator and re-warmed immediately before feeding. Feeds should not be left
warming for more than 15 minutes as re-warming for extended periods means that the feed will be held at a
temperature that is ideal for the growth of harmful bacteria. Holding feed in bottle warmers for lengthy periods
was reported as one of the probable causes of an outbreak of E. sakazakii infection (Gurtler, Kornacki and
Beuchat, 2005).
2.2.11 Transporting prepared feeds
In many care settings, feeds are prepared in a central preparation area, and transported to different wards
or areas of the facility. Transporting prepared feeds poses a risk of infection as it increases the time from
preparation to consumption, providing the opportunity for growth of harmful bacteria.
Because of this potential for growth, feeds that will not be consumed within two hours of preparation should
be quickly cooled and refrigerated until the temperature of the feed is reduced to a temperature of no more
than 5 °C. The cooled formula can then be transported to its destination. At the destination, feeds can be re-
warmed for feeding (Section 2.2.10). Alternatively, feeds can be returned to a refrigerator and used within 24
hours from preparation.
If transport takes longer than 30 minutes, it is recommended that feeds are transported under refrigerated
conditions in order to prevent feeds from warming up. If refrigerated transport is not available, feeds can be
transported in a cooled container, such as a cool bag containing ice-packs.
19. Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 3: In The Home
3.1 Recommendations 16
3.1.1 Cleaning and sterilizing feeding and preparation equipment 16
3.1.2 Preparing a feed using powdered infant formula 17
3.1.3 Preparing feeds in advance for later use 17
3.1.4 Re-warming stored feeds 18
3.1.5 Transporting feeds 18
3.2 Rationale behind recommendations 18
3.2.1 Good hygienic practice 18
3.2.2 Cleaning and sterilizing feeding and preparation equipment 18
3.2.3 Temperature of water for reconstitution 19
3.2.4 Storage of prepared feeds 19
3.2.5 Re-warming and use of stored feeds 19
3.2.6 Transporting feeds 19
3.2.7 Holding and feeding times 20
20. Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 3: In The Home
3.1 Recommendations
PIF is not a sterile product and may be contaminated with pathogens that can cause serious illness. Correct
preparation and handling reduces the risk of illness.
Where available, commercially sterile ready-to-feed liquid infant formula should be used for infants at greatest risk.
PIF is not a sterile product and can pose a risk to infants, particularly if it is prepared and handled
inappropriately. Reconstituted PIF provides an ideal environment for the growth of harmful bacteria. Even if
present in powdered formula at very low levels, inappropriate preparation and handling of feeds provides ideal
conditions for the growth of harmful bacteria, which greatly increases the risk of infection. However, the risk
can be reduced if feeds are prepared and handled correctly.
The recommendations below outline the best practice for the safe preparation, storage and handling of PIF in
the home in order to reduce the risk of infection with E. sakazakii. These recommendations are also appropriate
for reducing the risk of infection with Salmonella.
It is recommended healthcare professionals ensure that parents and caregivers are instructed in the safe
preparation, storage and handling of PIF.
3.1.1 Cleaning and sterilizing feeding and preparation equipment
It is very important that all equipment used for feeding infants and for preparing feeds has been thoroughly
cleaned and sterilized before use.
1. Hands should always be washed thoroughly with soap and water before cleaning and sterilizing feeding and
preparation equipment (as described below).
2. Cleaning: wash feeding and preparation equipment (e.g. cups, bottles, teats and spoons) thoroughly in hot
soapy water. Where feeding bottles are used, clean bottle and teat brushes should be used to scrub inside
and outside of bottles and teats to ensure that all remaining feed is removed.
3. After washing the feeding and preparation equipment, rinse thoroughly in safe water.
4. Sterilizing: if using a commercial home sterilizer (e.g. electric or microwave steam sterilizer, or chemical
sterilizer), follow manufacturer's instructions. Feeding and preparation equipment can also be sterilized by
boiling:
a. fill a large pan with water and completely submerge all washed feeding and preparation equipment,
ensuring there are no trapped air bubbles;
b. cover the pan with a lid and bring to a rolling boil, making sure the pan does not boil dry; and
c. keep the pan covered until the feeding and preparation equipment is needed.
5. Hands should be washed thoroughly with soap and water before removing feeding and preparation
equipment from a sterilizer or pan. The use of sterilized kitchen tongs for handling sterilized feeding and
preparation equipment is recommended.
6. To prevent recontamination, it is best to remove feeding and preparation equipment just before it is to be
used. If equipment is removed from the sterilizer and not used immediately, it should be covered and stored
in a clean place. Feeding bottles can be fully assembled to prevent the inside of the sterilized bottle and the
inside and outside of the teat from becoming contaminated.
21. Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 3: In The Home (continued)
3.1.2 Preparing a feed using powdered infant formula
It is best to make PIF fresh for each feed and to consume immediately, as reconstituted PIF provides ideal
conditions for the growth of harmful bacteria. The steps below outline the safest way to prepare individual
feeds of PIF in bottles or in feeding cups for immediate consumption.
1. Clean and disinfect a surface on which to prepare the feed.
2. Wash hands with soap and water, and dry using a clean cloth or a single-use napkin.
3. Boil a sufficient volume of safe water. If using an automatic kettle, wait until the kettle switches off;
otherwise make sure that the water comes to a rolling boil. Note: bottled water is not sterile and must be
boiled before use. Microwaves should never be used in the preparation of PIF as uneven heating may result
in 'hot spots' that can scald the infant's mouth.
4. Taking care to avoid scalds, pour the appropriate amount of boiled water that has been allowed to cool
to no less than 70 °C, into a cleaned and sterilized feeding cup or bottle. To achieve this temperature, the
water should be left for no more than 30 minutes after boiling.
5. To the water, add the exact amount of formula as instructed on the label. Adding more or less powder than
instructed could make infants ill.
a. If using bottles: assemble the cleaned and sterilized parts of the bottle according to the manufacturer's
instructions. Shake or swirl gently until the contents are mixed thoroughly, taking care to avoid scalds.
b. If using feeding cups: mix thoroughly by stirring with a cleaned and sterilized spoon, taking care to avoid
scalds.
6. Immediately after preparation, quickly cool feeds to feeding temperature by holding the bottle or feeding
cup under running tap water, or by placing in a container of cold or iced water. Ensure that the level of the
cooling water is below the top of the feeding cup or the lid of the bottle.
7. Dry the outside of the feeding cup or bottle with a clean or disposable cloth.
8. Because very hot water has been used to prepare the feed, it is essential that the feeding temperature is
checked before feeding in order to avoid scalding the infant's mouth. If necessary, continue cooling as
outlined in step 6.
9. Discard any feed that has not been consumed within two hours.
3.1.3 Preparing feeds in advance for later use
It is best to make PIF fresh for each feed and to consume immediately, as reconstituted PIF provides ideal
conditions for the growth of harmful bacteria. For practical reasons, however, feeds may need to be prepared in
advance. The steps below outline the safest way to prepare and store feeds for later use. If refrigeration is not
available, feeds should be prepared fresh and consumed immediately rather than prepared in advance for later use.
1. Follow steps 1 to 7 of Section 3.1.2. If using feeding cups, a batch of formula should be prepared in a clean,
sterile jar that is no larger than 1 litre, with a lid. The prepared PIF can be refrigerated and dispensed into
cups as needed.
2. Place cooled feeds in a refrigerator. The temperature of the refrigerator should be no higher than 5 °C.
3. Feeds can be stored in the refrigerator for up to 24 hours.
22. Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 3: In The Home (continued)
3.1.4 Re-warming stored feeds
1. Remove stored feed from the refrigerator just before it is needed.
2. Re-warm for no more than 15 minutes. To ensure that the feed heats evenly, periodically shake the covered
jar or container.
3. Microwave ovens should never be used to re-warm a feed as uneven heating may result in 'hot spots' that
can scald the infant's mouth.
4. Check feeding temperature in order to avoid scalding the infant's mouth.
5. Discard any re-warmed feed that has not been consumed within two hours.
3.1.5 Transporting feeds
Because of the potential for growth of harmful bacteria during transport, feeds should first be cooled to no
more than 5 °C in a refrigerator and then transported.
1. Prepare the feed and place in the refrigerator, as outlined in Section 3.1.3.
2. Ensure feed is cold before transporting.
3. Do not remove feed from the refrigerator until immediately before transporting.
4. Transport feed in a cool bag with ice packs.
5. Feeds transported in a cool bag should be used within two hours as cool bags do not always keep foods
adequately chilled.
6. Re-warm at the destination, as in Section 3.1.4.
7. If you reach the destination within two hours, feeds transported in a cool bag can be placed in a refrigerator
and held for up to 24 hours from the time of preparation.
8. Alternatively, if you are going out for the day, individual portions of PIF can be transported in washed and
sterilized containers. At the destination, hot water no less than 70 °C can be used to prepare the feed, using
washed and sterilized feeding and preparation equipment.
3.2 Rationale behind recommendations
3.2.1 Good hygienic practice
Poor hygiene has been reported as the probable cause of some E. sakazakii outbreaks (Forsythe, 2005). The
person preparing the feed should clean and disinfect the preparation surface and wash hands with soap and
water before preparing a feed. This is because harmful bacteria can be carried on hands and can also be
present on surfaces. Washing hands and cleaning and disinfecting surfaces reduces the risk of feeds becoming
contaminated during preparation.
Hands must also be washed after using the toilet and after diaper changing because harmful bacteria, including
E. sakazakii (Drudy et al., 2006), have been found in the urine and stools of infants. These bacteria can easily be
carried on the hands and contaminate feed during its preparation.
3.2.2 Cleaning and sterilizing feeding and preparation equipment
Outbreaks of E. sakazakii infection have been attributed to equipment used for preparing feeds (Gürtler et al.,
2005). E. sakazakii is widespread in the environment and has been shown to attach and grow (form 'biofilms')
on surfaces commonly used in infant feeding equipment, such as latex, silicon and stainless steel. It is therefore
important that all infant feeding and preparation equipment (e.g. feeding cups, bottles, rings and teats) has
been thoroughly cleaned and sterilized before use, since the formation of biofilms on such equipment may
result in reservoirs of infection that can continually contaminate feeds (Iversen, Lane and Forsythe, 2004).
23. Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 3: In The Home (continued)
3.2.3 Temperature of water for reconstitution
According to the FAO/WHO risk assessment, risk is dramatically reduced when PIF is reconstituted with water
that is no less than 70 °C, as this temperature will kill any E. sakazakii in the powder. This level of risk reduction
holds even if feeding times are extended (i.e. up to two hours), and even if ambient room temperature reaches
35 °C. Consequently, reconstituting PIF with water no less than 70 °C dramatically reduces the risk to all
infants, even slow feeding infants and infants in warm climates where refrigeration may not be readily available
(e.g. developing countries).
When PIF is prepared with water cooler than 70 °C, it does not reach a high enough temperature to completely
inactivate E. sakazakii present in the powder. This is a concern for two reasons: a) a small number of cells
may cause illness, therefore it is important that cells present in the PIF are destroyed; and b) the potential for
surviving cells to multiply in the reconstituted formula. This risk is increased when the reconstituted formula is
held for extended periods above refrigeration temperature.
Concerns have been raised over the use of very hot water for reconstituting PIF, but risk of E. sakazakii is only
dramatically reduced when water at a temperature of no less than 70 °C is used. Currently, the instructions
on many PIF products lead to PIF being reconstituted with water that is around 50 °C. But, according to the
FAO/WHO risk assessment, reconstitution with 50 °C water generally results in the greatest increase in risk,
unless the reconstituted formula is consumed immediately. Under no circumstances is risk reduced when PIF is
reconstituted with 50 °C water. Manufacturer's instructions should be reviewed in the light of the findings of
the risk assessment.
3.2.4 Storage of prepared feeds
Because PIF may contain harmful bacteria, it is best to prepare it fresh for each feed. However, in practical terms
this is not always possible. Feeds may need to be prepared in advance, e.g. for the crèche, babysitter, or if you
are going out for the day. In these circumstances, feeds should be prepared using water no less than 70 °C,
cooled quickly immediately after preparation, and stored in the refrigerator (at 5 °C or colder) for no more than
24 hours.
Feed stored in the refrigerator should be used within 24 hours of preparation. Even if water no less than 70 °C
was used to reconstitute PIF, spoilage bacteria may have survived that can grow at refrigeration temperatures
and can cause feeds to spoil. The quality of reconstituted PIF may also deteriorate on prolonged storage.
Feeds should be cooled quickly before placing into the refrigerator, as hot feeds will increase the refrigerator's
temperature. Feeds can be rapidly cooled by placing under cold running water or in a bowl of cold water.
3.2.5 Re-warming and use of stored feeds
Because of the possibility of growth of harmful bacteria at temperatures above 5 °C, stored formula should not
be removed from the refrigerator and re-warmed until immediately before feeding. Feeds should not be left
warming for more than 15 minutes as re-warming for extended periods means that the feed will be held at a
temperature that is ideal for the growth of harmful bacteria. Holding feed in bottle warmers for lengthy periods
was reported as one of the probable causes of an outbreak of E. sakazakii infection (Gurtler, Kornacki and
Beuchat, 2005).
3.2.6 Transporting feeds
Transporting prepared feeds poses a risk as it increases the time from preparation to consumption, providing
the opportunity for growth of harmful bacteria. Because of this potential for growth, feeds that need to be
transported should be quickly cooled and refrigerated until they are cold before transporting.
24. 0 Safe preparation, storage and handling of powdered infant formula - Guidelines
Part 3: In The Home (continued)
In order to minimize growth of harmful bacterial, cooled feeds should only be removed from the refrigerator
at the last minute and transported in a cool bag. At the destination, feeds can be re-warmed for feeding.
Feeds held in a cool bag should be used within two hours. Alternatively, if transported feeds are returned to a
refrigerator within two hours, they can be stored for up to 24 hours from preparation. By following these steps,
feeds will be kept cool, which will slow down or prevent the growth of harmful bacteria.
3.2.7 Holding and feeding times
Minimizing the time from preparation to consumption is an effective measure for controlling the risk of
infection with E. sakazakii. Prepared feed should be discarded after two hours, unless it has been stored in the
refrigerator since preparation (see Section 3.1.3). Leftover feed should never be saved for later, or added to a
freshly prepared feed, as harmful bacteria may have had the chance to grow during the feeding period.
It is recommended that formula is not held at room temperature for more than two hours, even if water at
no less than 70 °C is used to reconstitute PIF. This is because the feed may have become contaminated during
preparation, or harmful bacteria may have been introduced into the cup or feeding bottle from the infant's
mouth. Also, hot water (70 °C) may have activated bacterial spores of harmful bacteria in the formula. Holding
prepared feeds above refrigeration temperature for extended periods provides the opportunity for such bacteria
to grow.
25. Safe preparation, storage and handling of powdered infant formula - Guidelines
Appendix
Appendix 1 22
Appendix 2 23
Appendix 3 24
References cited in the text 25
26. Safe preparation, storage and handling of powdered infant formula - Guidelines
Appendix 1
Summary of recommendations made by the joint FAO/WHO expert meeting
(FAO/WHO, 2004):
• In situations where infants are not breastfed, caregivers, particularly of infants at high risk, should be
regularly alerted that PIF is not a sterile product and can be contaminated with pathogens that can cause
serious illness; they should be provided with information that can reduce the risk.
• In situations where infants are not breastfed, caregivers of high-risk infants, should be encouraged to use,
whenever possible and feasible, commercially sterile liquid formula or formula that has undergone an
effective point-of-use decontamination procedure (e.g. use of boiling water to reconstitute or by heating
reconstituted formula).
• Guidelines should be developed for the preparation, use and handling of infant formula to minimize risk.
• The infant-food industry should be encouraged to develop a greater range of commercially sterile alternative
formula products for high-risk groups.
• The infant-food industry should be encouraged to reduce the concentration and prevalence of E. sakazakii
in both the manufacturing environment and PIF. To this end, the infant-food industry should consider
implementing an effective environmental monitoring programme and the use of Enterobacteriaceae rather
than coliform testing as an indicator of hygienic control in factory production lines.
• In revising its code of practice, Codex should better address the microbiological risks of PIF and, if deemed
necessary, include the establishment of appropriate microbiological specifications for E. sakazakii in PIF.
• FAO/WHO should address the particular needs of some developing countries and establish effective
measures to minimize risk in situations where breast-milk substitutes may be used in exceptionally difficult
circumstances, e.g. feeding infants of HIV-positive mothers or low-birth-weight infants.
• The use of internationally validated detection and molecular typing methods for E. sakazakii and other
relevant microorganisms should be promoted.
• Investigation and reporting of sources and vehicles, including PIF, of infection by E. sakazakii and other
Enterobacteriaceae should be encouraged. This could include the establishment of a laboratory-based network.
• Research should be promoted to gain a better understanding of the ecology, taxonomy, virulence and other
characteristics of E. sakazakii and of ways to reduce its levels in reconstituted PIF.
27. Safe preparation, storage and handling of powdered infant formula - Guidelines
Appendix 2
Ten steps to successful breastfeeding
(Taken from the WHO/UNICEF Baby-Friendly Hospital Initiative (BFHI))
1. Have a written breastfeeding policy that is routinely communicated to all health care staff.
2. Train all health care staff in skills necessary to implement this policy.
3. Inform all pregnant women about the benefits and management of breastfeeding.
4. Help mothers initiate breastfeeding within one half-hour of birth.
5. Show mothers how to breastfeed and maintain lactation, even if they should be separated from
their infants.
6. Give newborn infants no food or drink other than breast milk, unless medically indicated.
7. Practice rooming in - that is, allow mothers and infants to remain together 24 hours a day.
8. Encourage breastfeeding on demand.
9. Give no artificial teats or pacifiers (also called dummies or soothers) to breastfeeding infants.
10. Foster the establishment of breastfeeding support groups and refer mothers to them on discharge
from the hospital or clinic.
28. Safe preparation, storage and handling of powdered infant formula - Guidelines
Appendix 3
The use of very hot water for the reconstitution of PIF has been questioned because of concerns over the loss
of heat-sensitive nutrients; the risk of scalding for infants and the preparer; activation of Bacillus cereus or
other bacterial spores; and clumping of powder (FAO/WHO, 2006). The ESPGHAN1 Committee on Nutrition
disagreed with the use of boiling water and of heating reconstituted formula to temperatures close to boiling
point, because of the possible adverse effects on nutrients such as vitamins (Agostoni et al., 2004). In October
2002, the US Department of Agriculture revised its recommendation to Health Professionals to use boiling
water to reconstitute PIF because of: the potential for the loss of heat sensitive nutrients; changes in physical
characteristics of some formulas; the inability to assure adequate destruction of E. sakazakii; and injury to
hospital staff preparing formula (US FDA, 2002). However, the UK has recently updated advice on preparation
of PIF, recommending reconstitution with water that is greater than 70 °C to reduce the risk associated with
using PIF (FSA, 2006).
The FAO/WHO expert meeting (2006) did note these concerns. Data presented at the meeting on the reduction
in vitamin levels on reconstitution with boiling water showed that vitamin C is the only vitamin that is
significantly affected (reduction ranging from 5.6 to 65.6% in the four powders tested). However, to offset the
loss of vitamins during the shelf-life of the product, all dry formulas actually contained higher levels of vitamin C
than was labelled. After reconstituting with boiling water, three of the four formulas still contained higher levels
than labelled. The fourth, after a vitamin C reduction of 65.6%, contained 9.0 mg/100 calories. This level is
still greater than the minimum level of vitamin C (8 mg/100 calories) required by the Codex Standard for Infant
Formula (CAC, 1981).
This study appears to indicate that reduction in vitamin levels from use of water at 70 °C is not significant.
However, as the results of only one study were discussed, the meeting did not agree to make any specific
recommendations on this issue, but it was noted that the option of fortifying formulas to accommodate
any reduction in vitamins could be possible if the practice of preparing formula with very hot water were
to be recommended.
In addressing the other concerns concerning the use of very hot water, the expert meeting concluded that the
risk of scalding can be addressed through educational messages on the label and training of those preparing
and feeding PIF. Although re-activation of spores may be an issue with the use of very hot water, using the
formula immediately, after cooling to a suitable feeding temperature, or refrigerating if it is to be used later,
should address this issue. Results of studies reported in a recent risk assessment (Food Standard Australia New
Zealand, 2003) show that the level of Bacillus cereus in the formula is not affected by the temperature of
water used (either 56°C or 90°C ) or the subsequent cooling conditions. The assessment also indicates that the
present guidance provided does not lead to a risk of Bacillus cereus. Finally, clumping does not occur with all
formulas when reconstituted with very hot water, and current technology could be applied to address this issue
for products where it does occur.
1 European Society for Paediatric Gastroenterology, Hepatology and Nutrition
29. Safe preparation, storage and handling of powdered infant formula - Guidelines
References Cited in the Text
Agostoni, C., Axelsson, I., Goulet, O., Koletzko, B., Michaelsen, K.F., Puntis, J.W.L. et al. 2004. Preparation
and handling of powdered infant formula: a commentary by the ESPGHAN Committee on Nutrition.
Journal of Pediatric Gasteroenterology and Nutrition, 39:320-322.
CAC [Codex Alimentarius Commission]. 1979. Recommended international code of hygienic practice
for foods for infants and children (CAC/RCP 21-1979).
See: http://www.codexalimentarius.net/web/standard_list do?lang=en
CAC. 1981. Codex Standard for Infant Formula (Codex Stan 72-1981).
See: http://www.codexalimentarius.net/web/standard_list do?lang=en
CAC. 1987. Codex Standard for Follow-up Formula (Codex Stan 156-1987).
See: http://www.codexalimentarius.net/web/standard_list do?lang=en
CAC. 2007. Codex Standard for Infant Formula and formulas for special medical Purposes Intended
for Infants (Codex Alinorm 07/30/26, Appendix II). (To be adopted in July 2007).
See: http://www.codexalimentarius.net/download/report/669/al30_26e.pdf
CAC. 2004. Report of the 25th Session of the Codex Committee on Nutrition and Foods for Special Dietary
Uses, Bonn, Germany, 3-7 November 2003. Codex Alinorm 04/27/26. Document J1464e.
Available from: http://www.fao.org/docrep/meeting/008/j1464e/j1464e00.htm
CDC [Centres for Disease Control and Prevention (USA)]. 2002. FoodNet annual report, 2002.
Available from: http://www.cdc.gov/foodnet/annual/2002/2002AnnualReport_tablesgraphs.pdf
Drudy, D., Mullane, N.R., Quinn, T., Wall, P.G. Fanning, S. 2006. Enterobacter sakazakii: an emerging
pathogen in powdered infant formula. Clinical Infectious Diseases, 42(7):996-1002.
FAO [Food and Agriculture Organization of the United Nations]/WHO [World Health Organization].
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Geneva, Switzerland, 2-5 February 2004. [FAO/WHO] Microbiological Risk Assessment Series, No. 6
FAO/WHO. 2006. Enterobacter sakazakii and Salmonella in powdered infant formula. Meeting Report. Joint
FAO/WHO Technical Meeting on Enterobacter sakazakii and Salmonella Powdered Infant Formula, Rome,
Italy, 16-20 January 2006. [FAO/WHO] Microbiological Risk Assessment Series, No. 10.
Forsythe, S. 2005. Enterobacter sakazakii and other bacteria in powdered infant milk formula. Maternal
and Child Nutrition, 1(1):44-50.
FSA [Food Standards Agency, UK]. 2006. Guidance on preparing infant formula. Article first posted 13 February
2006; accessed 25 November 2006.
Available at: http://www.food.gov.uk/news/newsarchive/2005/nov/infantformulastatementnov05
FSANZ [Food Standard Australia New Zealand]. 2003. Bacillus cereus in infant formula. Microbiological risk
assessment report.
30. Safe preparation, storage and handling of powdered infant formula - Guidelines
References Cited in the Text (continued)
Gurtler, J.B., Kornacki, J.L. Beuchat, L.R. 2005. Enterobacter sakazakii: A coliform of increased concern
to infant health. International Journal of Food Microbiology, 104(1):1-34.
Iversen, C., Lane, M. Forsythe, S.J. 2004. The growth profile, thermotolerance and biofilm formation of
Enterobacter sakazakii grown in infant formula milk. Letters in Applied Microbiology, 38(5):378-382.
Muytjens, H.L., Roelofs-Willemse, H. Jasper, G.H.J. 1988. Quality of powdered substitutes for breast milk
with regard to members of the family Enterobacteriaceae. Journal of Clinical Microbiology, 26:743-746.
US FDA [U.S. Food and Drug Administration]. 2002. Health Professionals Letter on Enterobacter sakazakii
infections associated with use of powdered (dry) infant formulas in neonatal intensive care units.
16-3-2006. See: http://www.cfsan.fda.gov/~dms/inf-ltr3.html
WHA [World Health Assembly]. 2005. Resolution WHA 58.32 on Infant and young-child nutrition.
See: http://www.who.int/gb/ebwha/pdf_files/WHA58/WHA58_32-en.pdf or http://www.who.int/gb/e/
e_wha58.html
WHO [World Health Organization]. 1981. International Code of Marketing of Breast-Milk Substitutes.
Available at: http://whqlibdoc.who.int/publications/9241541601.pdf
WHO/UNICEF [United Nations Children's Fund]. 2003. The Global Strategy for Infant and Young Child Feeding.
WHO, Geneva. See: www.who.int/child-adolescent-health/New_Publications/NUTRITION/gs_iycf.pdf