1) The study characterized synaptic functions of Extended Synaptotagmins (Esyt) mutants in Drosophila by analyzing the neuromuscular junction. In Esyt mutants, active zone density, mEPSP frequency, and mEPSP amplitudes increased, while quantal content decreased, indicating a presynaptic response. 2) Despite Esyt's role in calcium-dependent lipid transfer, mutants showed normal basal synaptic function and no changes in homeostasis. 3) Live imaging will further examine ER localization in Esyt mutants and calcium regulation of lipid exchange to help coordinate neurotransmission.