This document describes a liquid chromatographic method for the simultaneous quantitative determination of candesartan cilexetil and hydrochlorothiazide in pharmaceutical dosage forms. A simple and sensitive RP-HPLC method was developed and validated. The method allows for the separation, identification and quantification of candesartan cilexetil and hydrochlorothiazide in tablet formulations using a C18 column with UV detection. The method was validated in terms of specificity, linearity, precision, accuracy and robustness.
DEVELOPMENT AND VALIDATION OF AN RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATIO...rahul ampati
This document describes the development and validation of an RP-HPLC method for the simultaneous determination of ramipril and amlodipine in tablets. The method utilizes a gradient elution with a C18 column, mobile phase of acetonitrile and sodium perchlorate buffer, and UV detection. The method was validated per ICH guidelines and showed good linearity, accuracy, precision, specificity and robustness. Forced degradation studies demonstrated the method can separate ramipril, amlodipine and their degradation products. The method was successfully applied to determine the content of ramipril and amlodipine in three tablet batches.
This document describes the development and validation of an RP-HPLC method for the simultaneous estimation of atorvastatin and olmesartan tablets. The method was developed using a BDS hypersil C18 column with a mobile phase of ACN-TEA 40-60-0.1 pH 4.0 and detection at 235 nm. The method was validated for parameters such as linearity, precision, accuracy, robustness and system suitability and found to be suitable for the simultaneous quantification of atorvastatin and olmesartan in combined tablet dosage forms.
This document presents the development and validation of an RP-HPLC method for the simultaneous estimation of a muscle relaxant drug and analgesic drug in pure form and pharmaceutical dosage forms. It discusses conducting a literature review on existing methods, determining the physicochemical properties of the drugs, optimizing the analytical method, and validating the developed method as per ICH guidelines. The expected outcomes are an accurate, precise, simple, cost-effective and fast method for simultaneously analyzing the two drugs.
This document describes the development and validation of a stability-indicating RP-LC method for the determination of famciclovir (FCV) in the presence of its impurities and degradation products. A gradient reverse phase liquid chromatographic method was developed using an Inertsil ODS 3V column with a mobile phase of a potassium dihydrogen orthophosphate buffer and methanol mixture. FCV was subjected to oxidative, acid, base, hydrolytic, thermal, and photolytic degradation and was found to degrade significantly under oxidative, acid and base conditions. The developed method was validated according to ICH guidelines and demonstrated specificity, linearity, accuracy, precision, and robustness. This stability-indicating method can
The document presents a method development and validation for the quantitative estimation of ondansetron hydrochloride using high performance thin layer chromatography (HPTLC). An HPTLC method was developed using a methanol and triethylamine-glacial acetic acid mobile phase to separate ondansetron hydrochloride. The method was validated in terms of linearity, accuracy, precision, specificity, limit of detection, limit of quantitation, ruggedness and robustness. The developed and validated HPTLC method was successfully applied for the estimation of ondansetron hydrochloride in bulk drug samples and tablet dosage forms.
This document presents the development and validation of an RP-HPLC assay method for the simultaneous estimation of a muscle relaxant drug and analgesic drug in pure form and pharmaceutical dosage forms. It discusses conducting a literature review, determining drug profiles including solubility and wavelength absorption, optimizing the analytical method, and expected outcomes such as a simple, accurate, precise and cost-effective simultaneous estimation method. The method will be validated as per ICH guidelines and force degradation studies will be performed to evaluate the stability indicating properties of the method.
This document discusses analytical method development for pharmaceutical drug substances and products. It describes the importance of method development and outlines the key steps involved, including collecting sample information, selecting the chromatographic technique, stationary and mobile phases. It also discusses sources of impurities in drugs, control and qualification of impurities according to ICH guidelines, and pharmacopeial and ICH quality guidelines for impurity testing and thresholds.
DEVELOPMENT AND VALIDATION OF AN RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATIO...rahul ampati
This document describes the development and validation of an RP-HPLC method for the simultaneous determination of ramipril and amlodipine in tablets. The method utilizes a gradient elution with a C18 column, mobile phase of acetonitrile and sodium perchlorate buffer, and UV detection. The method was validated per ICH guidelines and showed good linearity, accuracy, precision, specificity and robustness. Forced degradation studies demonstrated the method can separate ramipril, amlodipine and their degradation products. The method was successfully applied to determine the content of ramipril and amlodipine in three tablet batches.
This document describes the development and validation of an RP-HPLC method for the simultaneous estimation of atorvastatin and olmesartan tablets. The method was developed using a BDS hypersil C18 column with a mobile phase of ACN-TEA 40-60-0.1 pH 4.0 and detection at 235 nm. The method was validated for parameters such as linearity, precision, accuracy, robustness and system suitability and found to be suitable for the simultaneous quantification of atorvastatin and olmesartan in combined tablet dosage forms.
This document presents the development and validation of an RP-HPLC method for the simultaneous estimation of a muscle relaxant drug and analgesic drug in pure form and pharmaceutical dosage forms. It discusses conducting a literature review on existing methods, determining the physicochemical properties of the drugs, optimizing the analytical method, and validating the developed method as per ICH guidelines. The expected outcomes are an accurate, precise, simple, cost-effective and fast method for simultaneously analyzing the two drugs.
This document describes the development and validation of a stability-indicating RP-LC method for the determination of famciclovir (FCV) in the presence of its impurities and degradation products. A gradient reverse phase liquid chromatographic method was developed using an Inertsil ODS 3V column with a mobile phase of a potassium dihydrogen orthophosphate buffer and methanol mixture. FCV was subjected to oxidative, acid, base, hydrolytic, thermal, and photolytic degradation and was found to degrade significantly under oxidative, acid and base conditions. The developed method was validated according to ICH guidelines and demonstrated specificity, linearity, accuracy, precision, and robustness. This stability-indicating method can
The document presents a method development and validation for the quantitative estimation of ondansetron hydrochloride using high performance thin layer chromatography (HPTLC). An HPTLC method was developed using a methanol and triethylamine-glacial acetic acid mobile phase to separate ondansetron hydrochloride. The method was validated in terms of linearity, accuracy, precision, specificity, limit of detection, limit of quantitation, ruggedness and robustness. The developed and validated HPTLC method was successfully applied for the estimation of ondansetron hydrochloride in bulk drug samples and tablet dosage forms.
This document presents the development and validation of an RP-HPLC assay method for the simultaneous estimation of a muscle relaxant drug and analgesic drug in pure form and pharmaceutical dosage forms. It discusses conducting a literature review, determining drug profiles including solubility and wavelength absorption, optimizing the analytical method, and expected outcomes such as a simple, accurate, precise and cost-effective simultaneous estimation method. The method will be validated as per ICH guidelines and force degradation studies will be performed to evaluate the stability indicating properties of the method.
This document discusses analytical method development for pharmaceutical drug substances and products. It describes the importance of method development and outlines the key steps involved, including collecting sample information, selecting the chromatographic technique, stationary and mobile phases. It also discusses sources of impurities in drugs, control and qualification of impurities according to ICH guidelines, and pharmacopeial and ICH quality guidelines for impurity testing and thresholds.
Development and validation of a stability-indicating HPLC method for the simultaneous determination of Losartan potassium, hydrochlorothiazide, and their degradationproducts
Development and Validation of Novel RP-HPLC method for the estimation of Nalo...Bhavana Gundavarapu
The RP-HPLC method was developed and validated for quantitative determination of Naloxegol in pharmaceutical dosage forms. The method was validated with regard to linearity, accuracy, precision, selectivity, and robustness. The method was applied successfully for the determination of Naloxegol during kinetic studies in aqueous solutions (pH and thermal degradation).
Stability indicating method development and validation for the simultaneous e...pharmaindexing
Stability indicating method development and validation for the simultaneous estimation of rabeprazole sodium and ketorolac tromethamine in bulk and synthetic mixture by RP-HPLC
This document describes the development and validation of an RP-HPLC method for the simultaneous analysis of diclofenac sodium and rabeprazole sodium without the need for an internal standard. The method utilizes a C8 column with a mobile phase of triethyl amine buffer (pH 5):acetonitrile (50:50 v/v) at a flow rate of 2 mL/min. Validation showed the method to be linear, accurate, precise, sensitive and stable. The method was applied to a pharmaceutical formulation containing both drugs with recoveries from 98-100%.
This document describes the development and validation of an RP-HPLC method for the quantification of flunarizine dihydrochloride. Key steps included selecting the instrument and optimizing conditions such as the column, mobile phase, and detection wavelength. The method was then validated based on parameters like accuracy, precision, specificity, detection limit, quantitation limit, and linearity range according to ICH guidelines. The proposed RP-HPLC method was found to be simple, accurate, precise and cost-effective for the analysis of flunarizine dihydrochloride in drug substances and pharmaceutical formulations.
Stability indicating rp hplc method development and validation for simultaneo...Rajasekhar
The document describes the development and validation of a stability-indicating RP-HPLC method for the simultaneous estimation of levodropropizine and chlorpheniramine maleate in bulk and pharmaceutical dosage forms. The method utilizes a C18 column with a mobile phase of 45% 0.1% orthophosphoric acid and 55% acetonitrile at a flow rate of 1 mL/min. The method was validated for parameters such as linearity, accuracy, precision, specificity, limit of detection, limit of quantification and robustness according to ICH guidelines. Forced degradation studies were also performed to demonstrate the stability-indicating ability of the developed method.
Here are the key IR frequencies identified in the sample that match the reference standard of propafenone:
- C-C stretch at 1186 cm-1
- C=C stretch at 1651 cm-1
- C-H stretch (symmetric) at 2939 cm-1
- C-H bend at 1328 cm-1
- CH2 stretch (symmetric) at 1369 cm-1
- CH2 bend at 1485 cm-1
- CH3 bend at 1398 cm-1
- C-O stretch at 1100 cm-1
- C=O stretch at 1695 cm-1
- N-H stretch at 3417 cm-1
The IR spectrum
This document presents a comparative study of three research papers on RP-HPLC method development and validation for the estimation of diclofenac sodium from tablet dosage forms. The objective is to determine the most effective and cost-efficient method. The materials and methods from each paper are compared based on preparation of standard and sample solutions, run time, and validation parameters. The results show that Paper 1 has the fastest run time of 10 minutes and is more accurate, precise, sensitive and cost-effective than Papers 2 and 3. Paper 1 uses a more optimal column dimension. In conclusion, the RP-HPLC method from Paper 1 is deemed superior for the quantitative analysis of diclofenac sodium in tablet dosage forms.
This document describes the development and validation of a UV spectrophotometric method for the estimation of methocarbamol in bulk and pharmaceutical dosage forms. The method was developed using acetone and 0.1N sodium hydroxide solution as solvents, in which methocarbamol is soluble. The drug has maximum absorbance at 267 nm. The method was validated as per ICH guidelines and was found to be linear, precise, accurate and specific. The developed method can be used for the quantitative analysis of methocarbamol in bulk and pharmaceutical formulations.
RP-HPLC method development and validation of ritonavir in bulk and pharmaceut...SriramNagarajan17
This document describes the development and validation of an RP-HPLC method for the quantification of the HIV protease inhibitor ritonavir (RIT) in bulk and pharmaceutical dosage forms. A simple isocratic RP-HPLC method was developed using a C18 column, mobile phase of 0.02M potassium dihydrogen phosphate buffer and acetonitrile (70:30 v/v), and detection at 237 nm. The method was validated per ICH guidelines and showed good linearity from 25-150 μg/mL, precision <0.5% RSD, accuracy of 99.3-100.6% recovery, and ability to quantify RIT in pharmaceutical tablets without interference from excipients.
Analytical method development and validation of tapentadol hcl by rp hplcShweta Tiwari
This document summarizes the development and validation of an analytical method using reverse phase high performance liquid chromatography (RP-HPLC) to analyze tapentadol hydrochloride in tablet dosage forms. The method utilized a C18 column with a mobile phase of methanol and water, detected the analyte at 272 nm. Validation of the method showed good linearity, precision, accuracy, specificity. The developed and validated method can be used for quality control of tapentadol hydrochloride tablet formulations.
Bioanalytical RP-HPLC Method Development and Validation for Estimation of Cur...Sagar Savale
The present study was aimed at developing a reversed phase high performance liquid chromatography (RPHPLC) method for determination of curcumin (CRM) in plasma and hydrochlorothiazide was used as an internal
standard. The separation was achieved by using C-18 column (Qualisil BDS C18, 250 mm x 4.6 mm I.D.)
coupled with a guard column of silica, mobile phase was consisting of acetonitrile: water with 0.1% formic acid
(40:60 v/v). The flow rate was 0.3 ml/min and the drug was detected using PDA detector at the wavelength of 423
nm. The experimental conditions, including the diluting solvent, mobile phase composition, column saturation
and flow rate, were optimised to provide high-resolution and reproducible peaks. The developed method was
validated in terms of linearity, recovery, precision, ruggedness, sensitivity (LOD and LOQ) and stability study
(short and long-term stabilities, Freeze/thaw stability and post-preparative).
Stability indicating analytical method development and validation for estimat...SriramNagarajan18
Stability indicating analytical method development and validation for estimation of Sacubitril and Valsartan in bulk and pharmaceutical dosage form using RP-HPLC
An emerging way of estimation of Olmesartan medoxomil & Hydrochlorothiazide i...Priyanka Bose
The document describes the development and validation of a UV spectrophotometric method for the simultaneous estimation of Olmesartan medoxomil and Hydrochlorothiazide in bulk and combined tablet dosage forms. The method was developed using absorbance correction and involved preparation of calibration curves for both drugs in the range of 1-20 μg/ml and 1-15 μg/ml, respectively. The method was validated as per ICH guidelines and was found to be precise, accurate, specific, robust and repeatable as indicated by the %RSD and %recovery values obtained during the validation experiments. The developed method can be effectively used for the routine quality control analysis of the drugs in pharmaceutical formulations.
The document describes a method development and validation project for the analysis of drugs using UV spectrophotometry and HPLC. It provides an introduction, objectives, literature review, and plan of work. The literature review summarizes 15 research papers on method development and validation using these techniques. The instruments available for the project are also listed. The conclusion states that the project aims to develop analytical methods for quality assessment of drug products to ensure therapeutic effectiveness.
Validation and method development of Apixaban A research project.Bhavana Gundavarapu
The document presents the development and validation of an HPLC-MS method for the estimation of Apixaban in human plasma. Key points:
- The aim was to develop a simple, precise and accurate HPLC-MS method for quantifying Apixaban levels in plasma using Apixaban-13C D3 as an internal standard.
- The method involved protein precipitation followed by HPLC-MS analysis on a C18 column with mobile phase containing acetonitrile, methanol and ammonium formate buffer at a flow rate of 0.4 mL/min and detection at m/z 459.3 and 462.3 for Apixaban and internal standard.
- The method was
Simultaneous estimation and validation of atenolol hydrochloro thiazide and l...srirampharma
This document describes the development and validation of a reverse phase HPLC method for the simultaneous estimation of atenolol, hydrochlorothiazide, and losartan K from pharmaceutical tablet dosage forms. The method utilizes a C18 column with a mobile phase of acetonitrile and phosphate buffer at a ratio of 70:30 delivered at 1.2 mL/min. The drugs were detected at 229 nm and provided good resolution and peak symmetry. The developed method was validated in terms of accuracy, precision, linearity, limits of detection and quantification, robustness, and solution stability. The method was applied to a commercial tablet dosage form and found to provide accurate results within 100% of the labeled claim for each drug.
IOSR Journal of Pharmacy (IOSRPHR), www.iosrphr.org, call for paper, research...iosrphr_editor
This document presents a stability-indicating reverse phase high performance liquid chromatography (RP-HPLC) method for the estimation of Valsartan in tablet dosage forms. The method uses a Phenomenox C18 column with a mobile phase of methanol and phosphate buffer at a ratio of 65:35 at a flow rate of 1mL/min. The method was validated for linearity, accuracy, precision, limit of detection, limit of quantification and specificity. Stress studies showed Valsartan degraded under acidic, basic, oxidative and heat conditions. The developed method can be used for assay of Valsartan in tablets and its degradation products.
Development and validation of a stability-indicating HPLC method for the simultaneous determination of Losartan potassium, hydrochlorothiazide, and their degradationproducts
Development and Validation of Novel RP-HPLC method for the estimation of Nalo...Bhavana Gundavarapu
The RP-HPLC method was developed and validated for quantitative determination of Naloxegol in pharmaceutical dosage forms. The method was validated with regard to linearity, accuracy, precision, selectivity, and robustness. The method was applied successfully for the determination of Naloxegol during kinetic studies in aqueous solutions (pH and thermal degradation).
Stability indicating method development and validation for the simultaneous e...pharmaindexing
Stability indicating method development and validation for the simultaneous estimation of rabeprazole sodium and ketorolac tromethamine in bulk and synthetic mixture by RP-HPLC
This document describes the development and validation of an RP-HPLC method for the simultaneous analysis of diclofenac sodium and rabeprazole sodium without the need for an internal standard. The method utilizes a C8 column with a mobile phase of triethyl amine buffer (pH 5):acetonitrile (50:50 v/v) at a flow rate of 2 mL/min. Validation showed the method to be linear, accurate, precise, sensitive and stable. The method was applied to a pharmaceutical formulation containing both drugs with recoveries from 98-100%.
This document describes the development and validation of an RP-HPLC method for the quantification of flunarizine dihydrochloride. Key steps included selecting the instrument and optimizing conditions such as the column, mobile phase, and detection wavelength. The method was then validated based on parameters like accuracy, precision, specificity, detection limit, quantitation limit, and linearity range according to ICH guidelines. The proposed RP-HPLC method was found to be simple, accurate, precise and cost-effective for the analysis of flunarizine dihydrochloride in drug substances and pharmaceutical formulations.
Stability indicating rp hplc method development and validation for simultaneo...Rajasekhar
The document describes the development and validation of a stability-indicating RP-HPLC method for the simultaneous estimation of levodropropizine and chlorpheniramine maleate in bulk and pharmaceutical dosage forms. The method utilizes a C18 column with a mobile phase of 45% 0.1% orthophosphoric acid and 55% acetonitrile at a flow rate of 1 mL/min. The method was validated for parameters such as linearity, accuracy, precision, specificity, limit of detection, limit of quantification and robustness according to ICH guidelines. Forced degradation studies were also performed to demonstrate the stability-indicating ability of the developed method.
Here are the key IR frequencies identified in the sample that match the reference standard of propafenone:
- C-C stretch at 1186 cm-1
- C=C stretch at 1651 cm-1
- C-H stretch (symmetric) at 2939 cm-1
- C-H bend at 1328 cm-1
- CH2 stretch (symmetric) at 1369 cm-1
- CH2 bend at 1485 cm-1
- CH3 bend at 1398 cm-1
- C-O stretch at 1100 cm-1
- C=O stretch at 1695 cm-1
- N-H stretch at 3417 cm-1
The IR spectrum
This document presents a comparative study of three research papers on RP-HPLC method development and validation for the estimation of diclofenac sodium from tablet dosage forms. The objective is to determine the most effective and cost-efficient method. The materials and methods from each paper are compared based on preparation of standard and sample solutions, run time, and validation parameters. The results show that Paper 1 has the fastest run time of 10 minutes and is more accurate, precise, sensitive and cost-effective than Papers 2 and 3. Paper 1 uses a more optimal column dimension. In conclusion, the RP-HPLC method from Paper 1 is deemed superior for the quantitative analysis of diclofenac sodium in tablet dosage forms.
This document describes the development and validation of a UV spectrophotometric method for the estimation of methocarbamol in bulk and pharmaceutical dosage forms. The method was developed using acetone and 0.1N sodium hydroxide solution as solvents, in which methocarbamol is soluble. The drug has maximum absorbance at 267 nm. The method was validated as per ICH guidelines and was found to be linear, precise, accurate and specific. The developed method can be used for the quantitative analysis of methocarbamol in bulk and pharmaceutical formulations.
RP-HPLC method development and validation of ritonavir in bulk and pharmaceut...SriramNagarajan17
This document describes the development and validation of an RP-HPLC method for the quantification of the HIV protease inhibitor ritonavir (RIT) in bulk and pharmaceutical dosage forms. A simple isocratic RP-HPLC method was developed using a C18 column, mobile phase of 0.02M potassium dihydrogen phosphate buffer and acetonitrile (70:30 v/v), and detection at 237 nm. The method was validated per ICH guidelines and showed good linearity from 25-150 μg/mL, precision <0.5% RSD, accuracy of 99.3-100.6% recovery, and ability to quantify RIT in pharmaceutical tablets without interference from excipients.
Analytical method development and validation of tapentadol hcl by rp hplcShweta Tiwari
This document summarizes the development and validation of an analytical method using reverse phase high performance liquid chromatography (RP-HPLC) to analyze tapentadol hydrochloride in tablet dosage forms. The method utilized a C18 column with a mobile phase of methanol and water, detected the analyte at 272 nm. Validation of the method showed good linearity, precision, accuracy, specificity. The developed and validated method can be used for quality control of tapentadol hydrochloride tablet formulations.
Bioanalytical RP-HPLC Method Development and Validation for Estimation of Cur...Sagar Savale
The present study was aimed at developing a reversed phase high performance liquid chromatography (RPHPLC) method for determination of curcumin (CRM) in plasma and hydrochlorothiazide was used as an internal
standard. The separation was achieved by using C-18 column (Qualisil BDS C18, 250 mm x 4.6 mm I.D.)
coupled with a guard column of silica, mobile phase was consisting of acetonitrile: water with 0.1% formic acid
(40:60 v/v). The flow rate was 0.3 ml/min and the drug was detected using PDA detector at the wavelength of 423
nm. The experimental conditions, including the diluting solvent, mobile phase composition, column saturation
and flow rate, were optimised to provide high-resolution and reproducible peaks. The developed method was
validated in terms of linearity, recovery, precision, ruggedness, sensitivity (LOD and LOQ) and stability study
(short and long-term stabilities, Freeze/thaw stability and post-preparative).
Stability indicating analytical method development and validation for estimat...SriramNagarajan18
Stability indicating analytical method development and validation for estimation of Sacubitril and Valsartan in bulk and pharmaceutical dosage form using RP-HPLC
An emerging way of estimation of Olmesartan medoxomil & Hydrochlorothiazide i...Priyanka Bose
The document describes the development and validation of a UV spectrophotometric method for the simultaneous estimation of Olmesartan medoxomil and Hydrochlorothiazide in bulk and combined tablet dosage forms. The method was developed using absorbance correction and involved preparation of calibration curves for both drugs in the range of 1-20 μg/ml and 1-15 μg/ml, respectively. The method was validated as per ICH guidelines and was found to be precise, accurate, specific, robust and repeatable as indicated by the %RSD and %recovery values obtained during the validation experiments. The developed method can be effectively used for the routine quality control analysis of the drugs in pharmaceutical formulations.
The document describes a method development and validation project for the analysis of drugs using UV spectrophotometry and HPLC. It provides an introduction, objectives, literature review, and plan of work. The literature review summarizes 15 research papers on method development and validation using these techniques. The instruments available for the project are also listed. The conclusion states that the project aims to develop analytical methods for quality assessment of drug products to ensure therapeutic effectiveness.
Validation and method development of Apixaban A research project.Bhavana Gundavarapu
The document presents the development and validation of an HPLC-MS method for the estimation of Apixaban in human plasma. Key points:
- The aim was to develop a simple, precise and accurate HPLC-MS method for quantifying Apixaban levels in plasma using Apixaban-13C D3 as an internal standard.
- The method involved protein precipitation followed by HPLC-MS analysis on a C18 column with mobile phase containing acetonitrile, methanol and ammonium formate buffer at a flow rate of 0.4 mL/min and detection at m/z 459.3 and 462.3 for Apixaban and internal standard.
- The method was
Simultaneous estimation and validation of atenolol hydrochloro thiazide and l...srirampharma
This document describes the development and validation of a reverse phase HPLC method for the simultaneous estimation of atenolol, hydrochlorothiazide, and losartan K from pharmaceutical tablet dosage forms. The method utilizes a C18 column with a mobile phase of acetonitrile and phosphate buffer at a ratio of 70:30 delivered at 1.2 mL/min. The drugs were detected at 229 nm and provided good resolution and peak symmetry. The developed method was validated in terms of accuracy, precision, linearity, limits of detection and quantification, robustness, and solution stability. The method was applied to a commercial tablet dosage form and found to provide accurate results within 100% of the labeled claim for each drug.
IOSR Journal of Pharmacy (IOSRPHR), www.iosrphr.org, call for paper, research...iosrphr_editor
This document presents a stability-indicating reverse phase high performance liquid chromatography (RP-HPLC) method for the estimation of Valsartan in tablet dosage forms. The method uses a Phenomenox C18 column with a mobile phase of methanol and phosphate buffer at a ratio of 65:35 at a flow rate of 1mL/min. The method was validated for linearity, accuracy, precision, limit of detection, limit of quantification and specificity. Stress studies showed Valsartan degraded under acidic, basic, oxidative and heat conditions. The developed method can be used for assay of Valsartan in tablets and its degradation products.
Differential spectrophotometric method for estimation and validation of verap...roshan telrandhe
The aimed of current research to development of the simple, rapid and sensitive Differential spectrophotometric method for the estimation of Verapamil in tablet dosage form. In this method two medium was use acid and alkaline and the difference spectrum was calculated. 0.1N HCL and 0.1N NaOH was used in this differential method. The λmax 278, Beers law limits 5-25µg/ml, regression equation Y= 0.024x-0.009, slope 0.024, intercept 0.09, correlation coefficient (r2) 0.998, %RSD <1.5, % Recovery (Tablet) 100.46% was shows the good efficacy and results. This method future scope in quality control of the verapamil in simple, precise and economically and it recommended for the routine drug quality analysis investigation
Structural elucidation, Identification, quantization of process related impur...IOSR Journals
Major process related unknown impurity associated with the synthesis of Hydralazine hydrochloride bulk drug was detected by high performance liquid chromatography (HPLC) and was subjected to high resolution accurate liquid chromatography mass spectroscopy (HR/AM-LCMS) for identification. The proposed impurity was isolated from Hydralazine hydrochloride active pharmaceutical ingredient (API) by preparative chromatographic method and was injected on HPLC for comparison of retention time with that of the unknown process related impurity in Hydralazine hydrochloride. The molecular ion peak of preparatively isolated impurity and that of unknown process related impurity in Hydralazine hydrochloride were compared for confirmation. The postulated structure was unambiguously confirmed with the help of HR/AM- LC MS/MS, NMR and FTIR data proposed to be 1-(2-phthalazin-1-ylhydrazino)phthalazine (Hazh Dimer). This impurity of Hydralazine hydrochloride is not been previously reported. A rapid Acquity H-class gradient method with runtime of 15.0min was developed for Quantitation on Unisphere Cyno column and validated for parameters such as accuracy, precision, linearity and range, robustness. The LOD and LOQ of method were 0081% and 0.0246% respectively.
This document describes a spectrofluorimetry method for determining nalidixic acid concentrations in human plasma. The method involves extracting nalidixic acid from plasma samples using chloroform and analyzing the samples using a spectrofluorimeter. The method was used to analyze plasma samples from a study comparing the bioavailability of a new generic nalidixic acid tablet formulation to a standard brand name formulation. Results of in vitro tests like dissolution and assay showed the new generic formulation was comparable to the standard. Pharmacokinetic analysis of plasma concentration data from subjects who received the formulations found no significant differences between the products.
If revisions are requested, the authors need to address the reviewer's comments and make the necessary changes to the paper. The revised paper is then resubmitted to the journal or conference for another round of review or evaluation of the journalism research paper.
Analytical method development and validation for the estimation of quinapril ...SriramNagarajan19
A simple and selective LC method is described for the determination of Quinapril and Tolcapone tablet dosage forms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a Mixed Phosphate buffer (KH2PO4 +K2HPO4): Acetonitrile 40:60, with detection of 239 nm. Linearity was observed in the range 50 - 150 µg /ml for Quinapril (r2 =0.995) and 62.5- 187.5µg /ml for Tolcapone (r2 =0.999) for the amount of drugs estimated by the proposed methods was in good agreement with the label claim.
The proposed methods were validated. The accuracy of the methods was assessed by recovery studies at three different levels. Recovery experiments indicated the absence of interference from commonly encountered pharmaceutical additives. The method was found to be precise as indicated by the repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the methods and can be used for routine analysis of pharmaceutical dosage form.
Novel RP-HPLC Method for Simultanious Determination of Sitagliptin and Simvas...iosrphr_editor
A simple, rapid, accurate, precise and novel high-performance liquid chromatographic method for simultaneous analysis of Sitagliptin and SIMV in pharmaceutical dosage form has been developed and validated. The chromatographic separation was accomplished on Welchrom RP-C18 Column (250 mm X 4.6 mm; 5μm), Shimadzu LC-20AT Prominence Liquid Chromatograph and with a mixture of 10 mM Phosphate buffer: acetonitrile and methanol in the range of (45:35:20 v/v/v). The flow rate was fixed at 1mL/minute and the analysis was performed using Shimadzu SPD-20A Prominence UV-detection was performed at 255 nm. The Sitagliptin and Simvastatin were separated within seven minutes. The retention time for SITA and SIMV was found to be 3.352 minutes and 5.402 minutes respectively. The calibration plots were linear over the concentration range of 10-50 μg/mL for SITA (r2 = 0.9998) and 4-20 μg/mL for SIMV (r2 = 0.9999). There was no interference due to commonly used excipients. The relative standard deviation for inter-day precision was lower than 2.0 % which obviously indicates that the present method was said to be highly precise. Regarding accuracy of the developed method the % RSD were also found less than 2 % which shows the method is completely accurate. The method was very sensitive with regard to LOD 0.681 μg/mL, 0.116 μg/mL and LOQ 2.250 μg/mL, 0.384 μg/mL respectively. The mean assay values for SITA and SIMV were determined in tablet dosage form were found to be within limits. The developed RP HPLC method was found to be simple, rapid, sensitive, highly precise and accurate highly suitable for routine analysis of drug samples containing Sitagliptin and Simvastatin.
1. A new RP-HPLC method was developed and validated for the estimation of Clopidogrel bisulphate in bulk drug and pharmaceutical formulations.
2. The method involved using a C18 column, mobile phase of acetonitrile and phosphate buffer at a ratio of 60:40, and detection at 224 nm.
3. The method was found to be linear, precise, accurate, specific, robust and suitable for the routine analysis of Clopidogrel bisulphate in quality control.
Method Development and Validation of Clopidogrel Bisulphate by Reverse Phase-...SriramNagarajan15
A new, simple sensitive, rapid, accurate and precise RP-HPLC method was developed for the estimation of Clopidogrel bisulphate in bulk drug and pharmaceutical formulation. Clopidogrel bisulphate was chromatographed on a reverse phase C18column (150 mm x 4.5 mm, i.d 5μm) in a mobile phase consisting of acetonitrile and phosphate buffer (pH: 3.0) in the ratio of 60:40 % v/v. The mobile phase was pumped at a flow rate of 1 ml/min with detection at 224 nm. The detector response was linear in the concentration of 50-150 μg /ml. The limit of detection and limit of quantitation was found to be 1.3 and 4.2 µg/ml, respectively. The intra and inter day variation was found to be less than 2%. The mean recovery of the drug from the solution was 99.79%. The proposed method is simple, fast, accurate, precise and reproducible hence, it can be applied for routine quality control analysis of Clopidogrel bisulphate in bulk drug and pharmaceutical formulation. Key words: Clopidogrel bisulphate, RP-HPLC, Validation, Accuracy, Precision.
Method Development and Validation of Clopidogrel Bisulphate by Reverse Phase-...SriramNagarajan15
A new, simple sensitive, rapid, accurate and precise RP-HPLC method was developed for the estimation of Clopidogrel bisulphate in bulk drug and pharmaceutical formulation. Clopidogrel bisulphate was chromatographed on a reverse phase C18column (150 mm x 4.5 mm, i.d 5μm) in a mobile phase consisting of acetonitrile and phosphate buffer (pH: 3.0) in the ratio of 60:40 % v/v. The mobile phase was pumped at a flow rate of 1 ml/min with detection at 224 nm. The detector response was linear in the concentration of 50-150 μg /ml. The limit of detection and limit of quantitation was found to be 1.3 and 4.2 µg/ml, respectively. The intra and inter day variation was found to be less than 2%. The mean recovery of the drug from the solution was 99.79%. The proposed method is simple, fast, accurate, precise and reproducible hence, it can be applied for routine quality control analysis of Clopidogrel bisulphate in bulk drug and pharmaceutical formulation. Key words: Clopidogrel bisulphate, RP-HPLC, Validation, Accuracy, Precision.
1. A new RP-HPLC method was developed and validated for the estimation of Clopidogrel bisulphate in bulk drug and pharmaceutical formulations.
2. The method involved using a C18 column, mobile phase of acetonitrile and phosphate buffer at a ratio of 60:40, and detection at 224 nm.
3. The method was found to be linear, precise, accurate, specific, robust and suitable for the routine analysis of Clopidogrel bisulphate in quality control labs.
Determination of Etodolac in Commercial Formulations by HPLC-UV Methodijtsrd
The aim of this study was to develop and verify a simple, rapid and sensitive high performance liquid chromatography method coupled with UV detector HPLC UV method for the quantitative determination of etodolac in bulk and pharmaceutical dosage forms. Chromatographic separation was performed at ambient conditions on a reverse phase ACE C8 analytical column 250 mm x 4.6 mm ID, 5 umm using the mobile phase containing acetonitrile water 80 20, v v at a flow rate of 1.0 mL min 1. A wavelength of 272 nm was used for etodolok and paracetamol IS . A retention time of 4.21 min and 2.02 min were obtained for etodolac and IS, respectively. The method showed linearity in the range of 0.08 10 µg mL 1 for etodolac R = 0.9999 . The linear regression equations obtained by least square regression method were the ratio of peak area of etodolac and IS =1.559 concentration etodolac µg mL 0.139. The intra day and inter day RE and RSD values of the method were =10.0 and =2.65 , respectively. Limit of detection LOD and limit of quantification LOQ were found to be 0.04 and 0.06 µg mL 1 for etodolac, respectively. A new, simple and sensitive high performance liquid chromatography method was developed and validated for etodolac. The method can be applied for the quantification of etodolac without derivatization in bulk solutions and commercial formulations using the internal standard. Tugrul Cagri Akman | Yucel Kadioglu "Determination of Etodolac in Commercial Formulations by HPLC-UV Method" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-1 , December 2019, URL: https://www.ijtsrd.com/papers/ijtsrd29452.pdfPaper URL: https://www.ijtsrd.com/pharmacy/analytical-chemistry/29452/determination-of-etodolac-in-commercial-formulations-by-hplc-uv-method/tugrul-cagri-akman
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
The current study was performed to develop and validate stability indicating high performance liquid chromatography method (RP-HPLC) for determination of ledipasvir (LPR); to identify and characterize its major degradants by liquid chromatographic– tandem mass spectrometric method (LC-ESI-MS).
Application of High Performance Thin Layer Chromatography with Densitometry f...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
This document describes the development and validation of a stability-indicating RP-HPLC method for the analysis of Methylphenidate Hydrochloride (MPH) and its impurities in pharmaceutical formulations. The method uses a C18 column with a mobile phase of methanol, acetonitrile and buffer at pH 4. MPH and its two impurities were well separated within 6 minutes. The method was validated per ICH guidelines and shown to be accurate, precise, linear, robust and stability-indicating. It can be used to analyze MPH content and degradation products in quality control testing.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
How to Make a Field Mandatory in Odoo 17Celine George
In Odoo, making a field required can be done through both Python code and XML views. When you set the required attribute to True in Python code, it makes the field required across all views where it's used. Conversely, when you set the required attribute in XML views, it makes the field required only in the context of that particular view.
ISO/IEC 27001, ISO/IEC 42001, and GDPR: Best Practices for Implementation and...PECB
Denis is a dynamic and results-driven Chief Information Officer (CIO) with a distinguished career spanning information systems analysis and technical project management. With a proven track record of spearheading the design and delivery of cutting-edge Information Management solutions, he has consistently elevated business operations, streamlined reporting functions, and maximized process efficiency.
Certified as an ISO/IEC 27001: Information Security Management Systems (ISMS) Lead Implementer, Data Protection Officer, and Cyber Risks Analyst, Denis brings a heightened focus on data security, privacy, and cyber resilience to every endeavor.
His expertise extends across a diverse spectrum of reporting, database, and web development applications, underpinned by an exceptional grasp of data storage and virtualization technologies. His proficiency in application testing, database administration, and data cleansing ensures seamless execution of complex projects.
What sets Denis apart is his comprehensive understanding of Business and Systems Analysis technologies, honed through involvement in all phases of the Software Development Lifecycle (SDLC). From meticulous requirements gathering to precise analysis, innovative design, rigorous development, thorough testing, and successful implementation, he has consistently delivered exceptional results.
Throughout his career, he has taken on multifaceted roles, from leading technical project management teams to owning solutions that drive operational excellence. His conscientious and proactive approach is unwavering, whether he is working independently or collaboratively within a team. His ability to connect with colleagues on a personal level underscores his commitment to fostering a harmonious and productive workplace environment.
Date: May 29, 2024
Tags: Information Security, ISO/IEC 27001, ISO/IEC 42001, Artificial Intelligence, GDPR
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LAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UPRAHUL
This Dissertation explores the particular circumstances of Mirzapur, a region located in the
core of India. Mirzapur, with its varied terrains and abundant biodiversity, offers an optimal
environment for investigating the changes in vegetation cover dynamics. Our study utilizes
advanced technologies such as GIS (Geographic Information Systems) and Remote sensing to
analyze the transformations that have taken place over the course of a decade.
The complex relationship between human activities and the environment has been the focus
of extensive research and worry. As the global community grapples with swift urbanization,
population expansion, and economic progress, the effects on natural ecosystems are becoming
more evident. A crucial element of this impact is the alteration of vegetation cover, which plays a
significant role in maintaining the ecological equilibrium of our planet.Land serves as the foundation for all human activities and provides the necessary materials for
these activities. As the most crucial natural resource, its utilization by humans results in different
'Land uses,' which are determined by both human activities and the physical characteristics of the
land.
The utilization of land is impacted by human needs and environmental factors. In countries
like India, rapid population growth and the emphasis on extensive resource exploitation can lead
to significant land degradation, adversely affecting the region's land cover.
Therefore, human intervention has significantly influenced land use patterns over many
centuries, evolving its structure over time and space. In the present era, these changes have
accelerated due to factors such as agriculture and urbanization. Information regarding land use and
cover is essential for various planning and management tasks related to the Earth's surface,
providing crucial environmental data for scientific, resource management, policy purposes, and
diverse human activities.
Accurate understanding of land use and cover is imperative for the development planning
of any area. Consequently, a wide range of professionals, including earth system scientists, land
and water managers, and urban planners, are interested in obtaining data on land use and cover
changes, conversion trends, and other related patterns. The spatial dimensions of land use and
cover support policymakers and scientists in making well-informed decisions, as alterations in
these patterns indicate shifts in economic and social conditions. Monitoring such changes with the
help of Advanced technologies like Remote Sensing and Geographic Information Systems is
crucial for coordinated efforts across different administrative levels. Advanced technologies like
Remote Sensing and Geographic Information Systems
9
Changes in vegetation cover refer to variations in the distribution, composition, and overall
structure of plant communities across different temporal and spatial scales. These changes can
occur natural.
বাংলাদেশের অর্থনৈতিক সমীক্ষা ২০২৪ [Bangladesh Economic Review 2024 Bangla.pdf] কম্পিউটার , ট্যাব ও স্মার্ট ফোন ভার্সন সহ সম্পূর্ণ বাংলা ই-বুক বা pdf বই " সুচিপত্র ...বুকমার্ক মেনু 🔖 ও হাইপার লিংক মেনু 📝👆 যুক্ত ..
আমাদের সবার জন্য খুব খুব গুরুত্বপূর্ণ একটি বই ..বিসিএস, ব্যাংক, ইউনিভার্সিটি ভর্তি ও যে কোন প্রতিযোগিতা মূলক পরীক্ষার জন্য এর খুব ইম্পরট্যান্ট একটি বিষয় ...তাছাড়া বাংলাদেশের সাম্প্রতিক যে কোন ডাটা বা তথ্য এই বইতে পাবেন ...
তাই একজন নাগরিক হিসাবে এই তথ্য গুলো আপনার জানা প্রয়োজন ...।
বিসিএস ও ব্যাংক এর লিখিত পরীক্ষা ...+এছাড়া মাধ্যমিক ও উচ্চমাধ্যমিকের স্টুডেন্টদের জন্য অনেক কাজে আসবে ...
it describes the bony anatomy including the femoral head , acetabulum, labrum . also discusses the capsule , ligaments . muscle that act on the hip joint and the range of motion are outlined. factors affecting hip joint stability and weight transmission through the joint are summarized.
Philippine Edukasyong Pantahanan at Pangkabuhayan (EPP) CurriculumMJDuyan
(𝐓𝐋𝐄 𝟏𝟎𝟎) (𝐋𝐞𝐬𝐬𝐨𝐧 𝟏)-𝐏𝐫𝐞𝐥𝐢𝐦𝐬
𝐃𝐢𝐬𝐜𝐮𝐬𝐬 𝐭𝐡𝐞 𝐄𝐏𝐏 𝐂𝐮𝐫𝐫𝐢𝐜𝐮𝐥𝐮𝐦 𝐢𝐧 𝐭𝐡𝐞 𝐏𝐡𝐢𝐥𝐢𝐩𝐩𝐢𝐧𝐞𝐬:
- Understand the goals and objectives of the Edukasyong Pantahanan at Pangkabuhayan (EPP) curriculum, recognizing its importance in fostering practical life skills and values among students. Students will also be able to identify the key components and subjects covered, such as agriculture, home economics, industrial arts, and information and communication technology.
𝐄𝐱𝐩𝐥𝐚𝐢𝐧 𝐭𝐡𝐞 𝐍𝐚𝐭𝐮𝐫𝐞 𝐚𝐧𝐝 𝐒𝐜𝐨𝐩𝐞 𝐨𝐟 𝐚𝐧 𝐄𝐧𝐭𝐫𝐞𝐩𝐫𝐞𝐧𝐞𝐮𝐫:
-Define entrepreneurship, distinguishing it from general business activities by emphasizing its focus on innovation, risk-taking, and value creation. Students will describe the characteristics and traits of successful entrepreneurs, including their roles and responsibilities, and discuss the broader economic and social impacts of entrepreneurial activities on both local and global scales.
This document provides an overview of wound healing, its functions, stages, mechanisms, factors affecting it, and complications.
A wound is a break in the integrity of the skin or tissues, which may be associated with disruption of the structure and function.
Healing is the body’s response to injury in an attempt to restore normal structure and functions.
Healing can occur in two ways: Regeneration and Repair
There are 4 phases of wound healing: hemostasis, inflammation, proliferation, and remodeling. This document also describes the mechanism of wound healing. Factors that affect healing include infection, uncontrolled diabetes, poor nutrition, age, anemia, the presence of foreign bodies, etc.
Complications of wound healing like infection, hyperpigmentation of scar, contractures, and keloid formation.
Walmart Business+ and Spark Good for Nonprofits.pdfTechSoup
"Learn about all the ways Walmart supports nonprofit organizations.
You will hear from Liz Willett, the Head of Nonprofits, and hear about what Walmart is doing to help nonprofits, including Walmart Business and Spark Good. Walmart Business+ is a new offer for nonprofits that offers discounts and also streamlines nonprofits order and expense tracking, saving time and money.
The webinar may also give some examples on how nonprofits can best leverage Walmart Business+.
The event will cover the following::
Walmart Business + (https://business.walmart.com/plus) is a new shopping experience for nonprofits, schools, and local business customers that connects an exclusive online shopping experience to stores. Benefits include free delivery and shipping, a 'Spend Analytics” feature, special discounts, deals and tax-exempt shopping.
Special TechSoup offer for a free 180 days membership, and up to $150 in discounts on eligible orders.
Spark Good (walmart.com/sparkgood) is a charitable platform that enables nonprofits to receive donations directly from customers and associates.
Answers about how you can do more with Walmart!"
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