general mechanism,overview of different medicines synthesized using this technology to treat different ailments and these medicines can be synthesized in a bulk.
This document discusses new pharmaceuticals derived from biotechnology. It begins by defining biotechnology as the use of living organisms or their components to develop products. It notes that biotechnology relies on disciplines like microbiology, biochemistry, and genetics. The document then discusses several biotechnology processes using bacteria, fungi, plants, insects, and mammalian cells to produce drugs. It provides examples of drug categories produced through biotechnology, including antibiotics, blood factors, hormones, growth factors, cytokines, enzymes, vaccines, and monoclonal antibodies. The summary highlights the multidisciplinary nature of biotechnology and its use of various organisms to produce important drug categories.
1. Molecular pharming involves using plants, plant cells, or plant tissues as bioreactors for the production of pharmaceuticals and other valuable proteins through recombinant DNA techniques.
2. Various systems have been used for molecular pharming including yeast, bacteria, plant viruses, transgenic plants, animal cell cultures, and transgenic animals. Plants have been engineered to produce vaccines, hormones, interferons, monoclonal antibodies, and other proteins.
3. Successful expression of proteins include cholera toxin B subunit and heat-labile enterotoxin in potatoes to induce immunization, hepatitis B surface antigen in tobacco, measles virus antigen in tobacco, growth hormone in tobacco chloroplasts, insulin in tobacco and lettuce, and interfer
It deals with application of such genes and proteins obtained from the animals especially for medicine and also industries. It is much useful to understand the basic.
Recombinant DNA technology allows scientists to isolate individual genes and insert them into other organisms. This has led to the production of recombinant proteins like human insulin, erythropoietin, and blood clotting factor VIII in microorganisms and cell cultures for medical use. The human insulin gene was isolated and inserted into plasmids in bacteria, which then mass-produced human insulin that could be purified and used as treatment for diabetes without side effects. Similarly, the genes for erythropoietin and factor VIII have been expressed in mammalian cells to produce these proteins for therapeutic purposes. Transgenic plants have also been engineered to produce recombinant proteins like the anti-coagulant hirudin in their tissues.
Production of therapeutic proteins in plantsUjala Ejaz
This document discusses the production of therapeutic proteins in plants. Therapeutic proteins are engineered proteins used for pharmaceutical purposes to treat diseases. Plants can be genetically modified to produce these proteins through nuclear or plastid transformation or transient expression. Examples of therapeutic proteins produced in plants include monoclonal antibodies in corn, HIV-suppressing proteins in spinach, and vaccines for hepatitis B in potato. While plant-based production has cost advantages over traditional methods, there are also bio-safety concerns regarding environmental contamination and food supply contamination that must be addressed.
This is about methods of creating transgenic animals,applications of transgenic animals in biotechnology and application of transgenic animals in pharmaceuticals.
The interleukins represent another large family of cytokines, with at least 25 different constituent members having been characterized thus far. Most of the interleukins are produced by a number of different cell types. Here, we introduce one of the important interleukins, interleukin-2. Contents contain interleukin-2 receptor, biological activity, cancer treatment, infectious diseases and Inhibition of interleukin-2 activity.
This document discusses new pharmaceuticals derived from biotechnology. It begins by defining biotechnology as the use of living organisms or their components to develop products. It notes that biotechnology relies on disciplines like microbiology, biochemistry, and genetics. The document then discusses several biotechnology processes using bacteria, fungi, plants, insects, and mammalian cells to produce drugs. It provides examples of drug categories produced through biotechnology, including antibiotics, blood factors, hormones, growth factors, cytokines, enzymes, vaccines, and monoclonal antibodies. The summary highlights the multidisciplinary nature of biotechnology and its use of various organisms to produce important drug categories.
1. Molecular pharming involves using plants, plant cells, or plant tissues as bioreactors for the production of pharmaceuticals and other valuable proteins through recombinant DNA techniques.
2. Various systems have been used for molecular pharming including yeast, bacteria, plant viruses, transgenic plants, animal cell cultures, and transgenic animals. Plants have been engineered to produce vaccines, hormones, interferons, monoclonal antibodies, and other proteins.
3. Successful expression of proteins include cholera toxin B subunit and heat-labile enterotoxin in potatoes to induce immunization, hepatitis B surface antigen in tobacco, measles virus antigen in tobacco, growth hormone in tobacco chloroplasts, insulin in tobacco and lettuce, and interfer
It deals with application of such genes and proteins obtained from the animals especially for medicine and also industries. It is much useful to understand the basic.
Recombinant DNA technology allows scientists to isolate individual genes and insert them into other organisms. This has led to the production of recombinant proteins like human insulin, erythropoietin, and blood clotting factor VIII in microorganisms and cell cultures for medical use. The human insulin gene was isolated and inserted into plasmids in bacteria, which then mass-produced human insulin that could be purified and used as treatment for diabetes without side effects. Similarly, the genes for erythropoietin and factor VIII have been expressed in mammalian cells to produce these proteins for therapeutic purposes. Transgenic plants have also been engineered to produce recombinant proteins like the anti-coagulant hirudin in their tissues.
Production of therapeutic proteins in plantsUjala Ejaz
This document discusses the production of therapeutic proteins in plants. Therapeutic proteins are engineered proteins used for pharmaceutical purposes to treat diseases. Plants can be genetically modified to produce these proteins through nuclear or plastid transformation or transient expression. Examples of therapeutic proteins produced in plants include monoclonal antibodies in corn, HIV-suppressing proteins in spinach, and vaccines for hepatitis B in potato. While plant-based production has cost advantages over traditional methods, there are also bio-safety concerns regarding environmental contamination and food supply contamination that must be addressed.
This is about methods of creating transgenic animals,applications of transgenic animals in biotechnology and application of transgenic animals in pharmaceuticals.
The interleukins represent another large family of cytokines, with at least 25 different constituent members having been characterized thus far. Most of the interleukins are produced by a number of different cell types. Here, we introduce one of the important interleukins, interleukin-2. Contents contain interleukin-2 receptor, biological activity, cancer treatment, infectious diseases and Inhibition of interleukin-2 activity.
The document discusses applications of recombinant DNA technology, focusing on important recombinant proteins and their uses. It provides details on the production of human insulin, interferons, and hepatitis B vaccine through recombinant DNA techniques. Human insulin was the first therapeutic protein produced via recombinant DNA, and is made by inserting the human insulin gene into E. coli bacteria. Interferons are produced recombinantly in yeast cells, which can properly glycosylate the proteins. The hepatitis B vaccine is made from antigenic proteins of the hepatitis B virus produced recombinantly, potentially through genetic engineering of banana plants.
Transgenic organisms are living organisms that contain genetic material from a different organism artificially introduced through recombinant DNA technology. The first transgenic animals were mice created in 1974. Transgenic microorganisms, animals, and plants have various applications and importance in areas like medicine, agriculture, industry, and the environment. However, they also raise political, economic, social, ethical and environmental concerns.
Interferons are proteins naturally produced by cells in response to viral infections and other pathogens. They were discovered in 1957 by Alick Isaacs and Jean Lindenmann who found that a substance from infected chicken cells interfered with viral replication. There are three main types of interferons - alpha, beta, and gamma - which have similar but distinct mechanisms of action involving inducing antiviral genes. Interferons are now used clinically to treat hepatitis C, various cancers, and multiple sclerosis. Recombinant and pegylated versions of interferons have been developed to improve pharmacokinetics. Combination therapy with ribavirin is also commonly used.
Dr. Lewis Teperman directs the Mary Lea Johnson Richards Organ Transplantation Center, where professionals analyze interferon and topics related to liver transplants. Interferons are proteins naturally produced by cells like white blood cells and epithelial cells that help the immune system combat harmful substances like viruses, bacteria, and cancer. There are three classes of interferons - alpha, beta, and gamma - that physicians can use to help treat diseases such as AIDS, leukemia, and hepatitis B.
This document discusses various therapeutic hormones including insulin, growth hormone, gonadotrophins, thyroid stimulating hormone, parathyroid hormone, and calcitonin. It provides details on their structure, function, production, formulations, and medical applications. Key points include: insulin is produced in the pancreas and regulates blood glucose; growth hormone stimulates growth; gonadotrophins like FSH and LH regulate reproduction; recombinant DNA technology is now used to produce many therapeutic hormones which has improved safety over extracts from animal tissues. These hormones are administered to treat various endocrine disorders and fertility issues.
This document discusses the production of recombinant therapeutic proteins. It outlines three main methods: microbial bioreactors like E. coli, mammalian cell culture bioreactors like CHO cells, and transgenic animal bioreactors. Transgenic animals are produced via DNA microinjection into embryos to incorporate expression vectors for target proteins. Their milk can then produce large quantities of complex proteins through scale-up. While advantageous for production scale, transgenic systems have limitations regarding animal health effects and post-translational modifications. Examples of therapeutic proteins produced include antithrombin in transgenic goats and alpha-1-antitrypsin in transgenic sheep.
Protein therapeutics can help treat diseases by replacing missing proteins, augmenting existing pathways, or providing novel functions. Insulin was one of the first successful protein therapeutics, but producing it recombinantly in E. coli addressed issues with sourcing and costs. While proteins are often well-tolerated and specific, challenges include stability, delivery, stimulating immune responses, and post-translational modifications. Advances in recombinant technology and production methods aim to overcome these challenges to develop more effective protein-based therapies.
Applications of Biotechnology in Animal Health
Biotechnology tools and products are useful in animal health research, agriculture, and veterinary medicine. Some key applications include:
1. Biotechnology-derived veterinary vaccines like recombinant vaccines which use genetic engineering to produce antigens without infectious agents. DNA vaccines are another type that use only DNA from pathogens.
2. Veterinary diagnostic systems using techniques like DNA/RNA probes and PCR for fast, sensitive, and specific disease diagnosis directly from samples.
3. Biotechnology-derived therapeutics including gene-deleted vaccines which use genetically engineered pathogens lacking virulence genes to induce immunity safely.
Interleukin-6 (IL-6) is a proinflammatory cytokine that plays a role in cancer progression and prognosis. It stimulates tumor growth through inhibiting apoptosis and promoting angiogenesis. Clinical studies have found that higher serum IL-6 levels correlate with advanced cancer stage and poorer patient survival for several cancers, including colorectal cancer, breast cancer, gastric cancer, and pancreatic cancer. IL-6 may serve as a prognostic biomarker for these malignancies.
Interferons and interleukins are cytokines that play an important role in the immune system. Interferons are proteins produced by cells in response to viral infections that activate immune responses in other cells. The three main types are alpha, beta, and gamma interferons. Interleukins are cytokines that are primarily produced by lymphocytes and macrophages to mediate communication between immune cells. There are over 30 identified interleukins that activate immune responses. Both interferons and interleukins have therapeutic applications for treating viral infections and cancers by enhancing immune activation. They are administered through intravenous or subcutaneous injection.
This document discusses therapeutic proteins, which are proteins engineered in laboratories for pharmaceutical use. It defines therapeutic proteins and outlines their attractive features like specificity and lower side effects compared to small molecule drugs. The document then covers the history and evolution of protein therapeutics, their classification, production methods using various cell types, obstacles in production, delivery systems, and some examples of marketed protein therapeutics.
Cytokines are small glycoproteins that act as signaling molecules between cells of the immune system. They are produced by a variety of immune cells including macrophages, monocytes, lymphocytes, and others. Cytokines function in both autocrine and paracrine manners through binding to specific cell surface receptors. They have a wide range of effects, including promoting or inhibiting inflammation, activating T cells and B cells, regulating hematopoiesis, and exhibiting anti-infective and anti-proliferative properties through interaction with their receptors on target cells. The functions of cytokines are pleiotropic, meaning they can have multiple effects on different cell types.
Periodontitis is a chronic infectious inflammatory disease caused by microbes; however the presence of microbes is not enough for the cause of its complex nature of disease. Inflammation is the prime cause of periodontal disease. It commences with the aggregation of pathogenic microbes that induce the host to stimulate a cascade of inflammatory response reactions which in-turn leads to the destruction of the host tissues itself. There is a complex interplay of innate and adaptive immune responses which fights against the pathogens by direct interaction or by release of certain molecules including cytokines.
Cytokines are cell signalling molecules that aid cell to cell communication in immune responses and stimulate the movement of cells towards sites of inflammation, infection and trauma. Cytokine biology reveals that there are some subsets of cytokines which are pro-inflammatory cytokines which stimulate the inflammatory responses and cause tissue destruction.
A periodontist is expected to have a sound basis of the cytokine profile to understand the pathogenesis of periodontitis and also to discover the new treatment modality of anti-cytokine therapy.
Initially, interleukins were thought to be produced by leukocytes and act on other leukocytes, leading to their name. Interleukins stimulate immune cell growth and differentiation and activate effector mechanisms. They are cytokines secreted by activated immune cells like macrophages and lymphocytes. Interleukins modulate immune and inflammatory responses.
This document provides information about the production of biopharmaceutical products. It begins with an introduction to biotechnology and defines it. It then discusses the development and fields of biotechnology. Specific biotech products like insulin and vaccines are described in detail, outlining their production processes which involve recombinant DNA technology. The document also discusses monoclonal antibodies, their uses as diagnostic tools, and the ELISA test method. It provides information on the production of monoclonal antibodies through hybridoma technology.
Cytokines are low molecular weight proteins or polypeptides that are secreted by cells and act as signaling molecules between cells to regulate immune responses and inflammation. They are classified into interleukins, interferons, tumor necrosis factors, chemokines, colony stimulating factors, and transforming growth factors. Cytokines bind to specific receptors on target cells and exert pleiotropic, redundant, synergistic or antagonistic effects through autocrine, paracrine or endocrine signaling. They are involved in processes like cell growth, differentiation, death, and induction or inhibition of other cytokines. Many cytokines have therapeutic uses like interferons in treating viral diseases and cancers, colony stimulating factors in treating neutropenia, and TNF inhibitors in treating autoimmune
Ethical issues related to transgenic animalsmahathiviji
This document discusses the ethical issues related to transgenic animals. It begins by defining ethics, bioethics, and the two types of genetic modification - altering genes normally present or transferring genes between individuals. Genetic modification of animals is used to help research human diseases, develop new drugs, provide transplant tissues/organs, and enhance livestock. However, this raises ethical concerns like unpredictable ecosystem impacts, animal welfare issues, risks to human and environmental health, "playing God", and religious concerns over gene transfers between species. The document also discusses regulation bodies like GEAC and issues around patents and biopiracy of genetically modified organisms.
Cytokine Receptors, Mohammad Mufarreh AliMMufarreh
A detailed description of the nature, types, and mechanisms of action of cytokine receptors.
Describes the different functions of cytokines and their role in the regulation of the immune response.
Cytokine receptor signalling and their regulation and the role of cytokines in disease is also covered briefly.
Biotechnology involves applying technology to modify organisms by adding genes from other species. Genetic engineering is the basic tool, involving isolating, modifying, and inserting genes into new species to develop transgenes. This allows developing organisms with novel traits like insect or herbicide resistance. Common methods are using Agrobacterium or gene guns to insert transformation cassettes containing the gene of interest, selectable markers, and insertion sequences. Pakistan uses Bt cotton containing genes for toxins killing bollworm pests. Researchers are also developing Bt rice containing genes for resistance to bacterial leaf blight diseases. Proponents argue GM foods are as safe or safer than conventional foods after regulatory testing and oversight.
CHAPTER 12 BIOTECHNOLOGY AND ITS APPLICATIONS.pptxJyoti Gadge
Genetically modified crops can increase food production and reduce reliance on pesticides. The document discusses biotechnological applications in agriculture including GM crops, such as Bt cotton, which are modified to produce toxins that kill pests without insecticides. It also covers applications in medicine like producing human insulin through genetic engineering of E. coli and using gene therapy to treat genetic diseases. Ethical issues around patenting native plants and related traditional knowledge are also addressed.
The document discusses applications of recombinant DNA technology, focusing on important recombinant proteins and their uses. It provides details on the production of human insulin, interferons, and hepatitis B vaccine through recombinant DNA techniques. Human insulin was the first therapeutic protein produced via recombinant DNA, and is made by inserting the human insulin gene into E. coli bacteria. Interferons are produced recombinantly in yeast cells, which can properly glycosylate the proteins. The hepatitis B vaccine is made from antigenic proteins of the hepatitis B virus produced recombinantly, potentially through genetic engineering of banana plants.
Transgenic organisms are living organisms that contain genetic material from a different organism artificially introduced through recombinant DNA technology. The first transgenic animals were mice created in 1974. Transgenic microorganisms, animals, and plants have various applications and importance in areas like medicine, agriculture, industry, and the environment. However, they also raise political, economic, social, ethical and environmental concerns.
Interferons are proteins naturally produced by cells in response to viral infections and other pathogens. They were discovered in 1957 by Alick Isaacs and Jean Lindenmann who found that a substance from infected chicken cells interfered with viral replication. There are three main types of interferons - alpha, beta, and gamma - which have similar but distinct mechanisms of action involving inducing antiviral genes. Interferons are now used clinically to treat hepatitis C, various cancers, and multiple sclerosis. Recombinant and pegylated versions of interferons have been developed to improve pharmacokinetics. Combination therapy with ribavirin is also commonly used.
Dr. Lewis Teperman directs the Mary Lea Johnson Richards Organ Transplantation Center, where professionals analyze interferon and topics related to liver transplants. Interferons are proteins naturally produced by cells like white blood cells and epithelial cells that help the immune system combat harmful substances like viruses, bacteria, and cancer. There are three classes of interferons - alpha, beta, and gamma - that physicians can use to help treat diseases such as AIDS, leukemia, and hepatitis B.
This document discusses various therapeutic hormones including insulin, growth hormone, gonadotrophins, thyroid stimulating hormone, parathyroid hormone, and calcitonin. It provides details on their structure, function, production, formulations, and medical applications. Key points include: insulin is produced in the pancreas and regulates blood glucose; growth hormone stimulates growth; gonadotrophins like FSH and LH regulate reproduction; recombinant DNA technology is now used to produce many therapeutic hormones which has improved safety over extracts from animal tissues. These hormones are administered to treat various endocrine disorders and fertility issues.
This document discusses the production of recombinant therapeutic proteins. It outlines three main methods: microbial bioreactors like E. coli, mammalian cell culture bioreactors like CHO cells, and transgenic animal bioreactors. Transgenic animals are produced via DNA microinjection into embryos to incorporate expression vectors for target proteins. Their milk can then produce large quantities of complex proteins through scale-up. While advantageous for production scale, transgenic systems have limitations regarding animal health effects and post-translational modifications. Examples of therapeutic proteins produced include antithrombin in transgenic goats and alpha-1-antitrypsin in transgenic sheep.
Protein therapeutics can help treat diseases by replacing missing proteins, augmenting existing pathways, or providing novel functions. Insulin was one of the first successful protein therapeutics, but producing it recombinantly in E. coli addressed issues with sourcing and costs. While proteins are often well-tolerated and specific, challenges include stability, delivery, stimulating immune responses, and post-translational modifications. Advances in recombinant technology and production methods aim to overcome these challenges to develop more effective protein-based therapies.
Applications of Biotechnology in Animal Health
Biotechnology tools and products are useful in animal health research, agriculture, and veterinary medicine. Some key applications include:
1. Biotechnology-derived veterinary vaccines like recombinant vaccines which use genetic engineering to produce antigens without infectious agents. DNA vaccines are another type that use only DNA from pathogens.
2. Veterinary diagnostic systems using techniques like DNA/RNA probes and PCR for fast, sensitive, and specific disease diagnosis directly from samples.
3. Biotechnology-derived therapeutics including gene-deleted vaccines which use genetically engineered pathogens lacking virulence genes to induce immunity safely.
Interleukin-6 (IL-6) is a proinflammatory cytokine that plays a role in cancer progression and prognosis. It stimulates tumor growth through inhibiting apoptosis and promoting angiogenesis. Clinical studies have found that higher serum IL-6 levels correlate with advanced cancer stage and poorer patient survival for several cancers, including colorectal cancer, breast cancer, gastric cancer, and pancreatic cancer. IL-6 may serve as a prognostic biomarker for these malignancies.
Interferons and interleukins are cytokines that play an important role in the immune system. Interferons are proteins produced by cells in response to viral infections that activate immune responses in other cells. The three main types are alpha, beta, and gamma interferons. Interleukins are cytokines that are primarily produced by lymphocytes and macrophages to mediate communication between immune cells. There are over 30 identified interleukins that activate immune responses. Both interferons and interleukins have therapeutic applications for treating viral infections and cancers by enhancing immune activation. They are administered through intravenous or subcutaneous injection.
This document discusses therapeutic proteins, which are proteins engineered in laboratories for pharmaceutical use. It defines therapeutic proteins and outlines their attractive features like specificity and lower side effects compared to small molecule drugs. The document then covers the history and evolution of protein therapeutics, their classification, production methods using various cell types, obstacles in production, delivery systems, and some examples of marketed protein therapeutics.
Cytokines are small glycoproteins that act as signaling molecules between cells of the immune system. They are produced by a variety of immune cells including macrophages, monocytes, lymphocytes, and others. Cytokines function in both autocrine and paracrine manners through binding to specific cell surface receptors. They have a wide range of effects, including promoting or inhibiting inflammation, activating T cells and B cells, regulating hematopoiesis, and exhibiting anti-infective and anti-proliferative properties through interaction with their receptors on target cells. The functions of cytokines are pleiotropic, meaning they can have multiple effects on different cell types.
Periodontitis is a chronic infectious inflammatory disease caused by microbes; however the presence of microbes is not enough for the cause of its complex nature of disease. Inflammation is the prime cause of periodontal disease. It commences with the aggregation of pathogenic microbes that induce the host to stimulate a cascade of inflammatory response reactions which in-turn leads to the destruction of the host tissues itself. There is a complex interplay of innate and adaptive immune responses which fights against the pathogens by direct interaction or by release of certain molecules including cytokines.
Cytokines are cell signalling molecules that aid cell to cell communication in immune responses and stimulate the movement of cells towards sites of inflammation, infection and trauma. Cytokine biology reveals that there are some subsets of cytokines which are pro-inflammatory cytokines which stimulate the inflammatory responses and cause tissue destruction.
A periodontist is expected to have a sound basis of the cytokine profile to understand the pathogenesis of periodontitis and also to discover the new treatment modality of anti-cytokine therapy.
Initially, interleukins were thought to be produced by leukocytes and act on other leukocytes, leading to their name. Interleukins stimulate immune cell growth and differentiation and activate effector mechanisms. They are cytokines secreted by activated immune cells like macrophages and lymphocytes. Interleukins modulate immune and inflammatory responses.
This document provides information about the production of biopharmaceutical products. It begins with an introduction to biotechnology and defines it. It then discusses the development and fields of biotechnology. Specific biotech products like insulin and vaccines are described in detail, outlining their production processes which involve recombinant DNA technology. The document also discusses monoclonal antibodies, their uses as diagnostic tools, and the ELISA test method. It provides information on the production of monoclonal antibodies through hybridoma technology.
Cytokines are low molecular weight proteins or polypeptides that are secreted by cells and act as signaling molecules between cells to regulate immune responses and inflammation. They are classified into interleukins, interferons, tumor necrosis factors, chemokines, colony stimulating factors, and transforming growth factors. Cytokines bind to specific receptors on target cells and exert pleiotropic, redundant, synergistic or antagonistic effects through autocrine, paracrine or endocrine signaling. They are involved in processes like cell growth, differentiation, death, and induction or inhibition of other cytokines. Many cytokines have therapeutic uses like interferons in treating viral diseases and cancers, colony stimulating factors in treating neutropenia, and TNF inhibitors in treating autoimmune
Ethical issues related to transgenic animalsmahathiviji
This document discusses the ethical issues related to transgenic animals. It begins by defining ethics, bioethics, and the two types of genetic modification - altering genes normally present or transferring genes between individuals. Genetic modification of animals is used to help research human diseases, develop new drugs, provide transplant tissues/organs, and enhance livestock. However, this raises ethical concerns like unpredictable ecosystem impacts, animal welfare issues, risks to human and environmental health, "playing God", and religious concerns over gene transfers between species. The document also discusses regulation bodies like GEAC and issues around patents and biopiracy of genetically modified organisms.
Cytokine Receptors, Mohammad Mufarreh AliMMufarreh
A detailed description of the nature, types, and mechanisms of action of cytokine receptors.
Describes the different functions of cytokines and their role in the regulation of the immune response.
Cytokine receptor signalling and their regulation and the role of cytokines in disease is also covered briefly.
Biotechnology involves applying technology to modify organisms by adding genes from other species. Genetic engineering is the basic tool, involving isolating, modifying, and inserting genes into new species to develop transgenes. This allows developing organisms with novel traits like insect or herbicide resistance. Common methods are using Agrobacterium or gene guns to insert transformation cassettes containing the gene of interest, selectable markers, and insertion sequences. Pakistan uses Bt cotton containing genes for toxins killing bollworm pests. Researchers are also developing Bt rice containing genes for resistance to bacterial leaf blight diseases. Proponents argue GM foods are as safe or safer than conventional foods after regulatory testing and oversight.
CHAPTER 12 BIOTECHNOLOGY AND ITS APPLICATIONS.pptxJyoti Gadge
Genetically modified crops can increase food production and reduce reliance on pesticides. The document discusses biotechnological applications in agriculture including GM crops, such as Bt cotton, which are modified to produce toxins that kill pests without insecticides. It also covers applications in medicine like producing human insulin through genetic engineering of E. coli and using gene therapy to treat genetic diseases. Ethical issues around patenting native plants and related traditional knowledge are also addressed.
Genetic engineering and pharmaceutical production in microorganismsNawfal Aldujaily
Role Of Genetic Engineering In Improvement Of Pharmaceutical Production of Microorganisms lecture in department of biology.faculty of science.University of Kufa
Cell lines are immortal cell cultures that are used extensively in biomedical research. Some key uses of cell lines include studying basic cell biology, testing drug toxicity, cancer research, virology studies, and gene therapy. Common cell lines include HeLa cells from cervical cancer tissue and MCF-7 cells from breast cancer tissue. Cell lines are also important in the production of vaccines, antibodies, and other biological products through recombinant protein expression in cultured cells. Respiratory and pancreatic beta cell lines have applications in research on inflammation, diabetes, and other diseases.
Cell lines are immortal cell cultures that are used extensively in biomedical research. Some key uses of cell lines include modeling disease and testing drug toxicity. Common cell lines include HeLa cells, MCF-7 breast cancer cells, and VERO monkey kidney cells. Cell lines are also used to produce biological products like vaccines, antibodies, and recombinant proteins. Respiratory cell lines help study lung diseases while pancreatic beta cell lines aid diabetes research. The duck cell line EB66 is being used commercially for viral vaccine production.
Applications of rdna technology in medicinesAdarsh Patil
Recombinant DNA technology has many applications in medicine. It allows for the production of important proteins like human insulin and growth hormones by inserting the gene for that protein into a host cell like E. coli. This is safer than extracting the protein from animal sources. Recombinant DNA also enables the development of vaccines by cloning the gene for the protective antigen, and production of monoclonal antibodies and interferon proteins through genetic engineering techniques. It has revolutionized diagnosis and treatment of diseases like diabetes, dwarfism, cancer, and HIV/AIDS.
This document discusses various applications of biotechnology including health care, agriculture, industrial, environmental and genomics applications. It specifically focuses on topics like recombinant protein production for diseases, genetically modified crops, tissue culture applications in plants, microbial biotechnology, gene therapy and genetically modified organisms. Examples of insulin production, vaccine production, transgenic plants for pest resistance and nutrition improvement, and plants as bioreactors are provided.
The document discusses various applications of genetic engineering in medicine. It describes how genetic engineering is used to produce insulin, vaccines, monoclonal antibodies, and other drugs through the manipulation of genes and transfer of genes between organisms. The document also explores the future potential of gene therapy and tissue engineering to treat genetic diseases and regenerate tissues.
A cell line is a product of immortal cells that are used for biological research.
Cells used for cell lines are immortal, that happens if a cell is cancerous.
The cells can perpetuate division indefinitely which is unlike regular cells which can only divide approximately 50 times.
Human cell lines
MCF-7 breast cancer
HL 60 Leukemia
HEK-293 Human embryonic kidney
HeLa Henrietta lacks
Primate cell lines
Vero African green monkey kidney epithelial cells
Cos-7 African green monkey kidney cells
And others such as CHO from hamster, sf9 & sf21 from insect cells.
Therapeutic proteins are proteins engineered for pharmaceutical use. They are produced through recombinant DNA technology and delivered to replace proteins deficient in certain illnesses. Therapeutic proteins are classified based on their pharmacological action, molecular type, and mechanism. They can be produced through microbial bioreactors, mammalian cell culture bioreactors, or by expressing the protein in the milk of transgenic animals. Common therapeutic proteins include hormones, clotting factors, vaccines, and monoclonal antibodies used to treat diseases like cancer, infections, hemophilia, and hepatitis.
Biotechnology uses living organisms or processes to develop products for human welfare. It includes green biotechnology for agriculture, red biotechnology for medicine, blue biotechnology for aquatic applications, and white biotechnology for industrial uses. Red biotechnology, also called health biotechnology, utilizes biotechnology in medicine through techniques like drug production, pharmacogenomics, gene therapy, and tissue engineering. These applications are improving healthcare by developing new medicines and therapies, personalizing drug treatment, correcting genetic defects, and engineering replacement tissues.
Pharmaceutical biotechnology combines pharmaceuticals and biotechnology to develop improved medicines. This is done through pharmacogenomics, which studies how genetics affect drug responses, allowing for personalized medicines. Some benefits include safer, more effective drugs designed for a person's genetic makeup. Common biopharmaceutical products include monoclonal antibodies, recombinant proteins, vaccines, and drugs produced through recombinant DNA techniques. When combined, pharmaceuticals and biotechnology can advance healthcare through more targeted disease treatment.
application of biotechnology in pharmaceuticalsSansarBookdepot
This document discusses pharmaceutical biotechnology and advanced drug delivery. It explains that biotechnology uses microorganisms, plants, or animal parts to produce useful compounds for pharmaceuticals. Recombinant DNA technology allows controlling gene expression in organisms used for manufacturing. This leads to genetically modified organisms that open new possibilities. The document also discusses biopharmaceuticals like proteins, antibodies, and recombinant DNA products. It describes how monoclonal antibodies are produced and outlines recombinant DNA technology using plasmids and vectors. Finally, it discusses considerations for formulating biotech products, including sterility, viral decontamination, pyrogen removal, and common excipients used in parenteral formulations.
Biopharmaceuticals can be produced in living systems like plants for therapeutic purposes. The use of plants can be more practical, safe and economical than other biological systems for producing proteins. Plants offer advantages for producing proteins through post-translational modification and are capable of glycosylation without assistance. Genetic engineering allows the insertion of DNA encoding a desired protein into plant cells to produce pharmaceutical proteins through transformation techniques like Agrobacterium tumefaciens-mediated transformation or biolistics. Proteins produced in plants can be used for applications like antibodies, vaccines, hormones and enzymes.
Recombinant DNA technology involves combining DNA from different sources in a way that does not occur naturally. This allows genes to be precisely analyzed and manipulated for various applications. Some key uses of recombinant DNA include producing human insulin and growth hormone to treat diabetes and growth disorders. Genetically modified crops have also been developed for increased pest resistance and herbicide tolerance to improve agriculture. Recombinant DNA is widely used in basic research and has generated many medical products as well as applications in agriculture, industry, and controlling mosquito-borne diseases.
Genetic engineering, also called genetic modification, is used to add new traits to organisms that do not naturally occur. The document discusses various applications of genetic engineering including in agriculture, medicine, and genetic studies. In agriculture, genetic engineering has been used to develop crops with traits like virus resistance, insect resistance, and herbicide tolerance. In medicine, genetic engineering is being used to produce human insulin, growth hormone, vaccines, and interferons. It is also being explored for gene therapy applications.
This document discusses biotechnology products produced through transgenic animals, plants, and bacteria. It provides examples of how each are used to develop pharmaceuticals, industrial chemicals, and other products that benefit humanity. Transgenic animals produce drugs, blood substitutes, and hormones. Transgenic plants are engineered for pest resistance, nutrition, and vaccine production. Transgenic bacteria synthesize insulin, vaccines, chemicals, and assist mining by extracting metals. Overall, the document outlines the broad applications of biotechnology across multiple disciplines to develop welfare products using genetic engineering techniques.
Chapter 12. biotechnology and its application2014 by mohanbiomohan bio
The document discusses several applications of biotechnology including:
1. Production of human insulin using recombinant DNA technology and other therapeutic products through genetic engineering.
2. Use of microbes and genetic engineering to produce antibiotics, degrade waste, and extract minerals.
3. Genetic engineering of plants through tissue culture and transgenic techniques to develop pest-resistant, drought-tolerant, and nutrient-enhanced crops.
4. Genetic engineering of animals through transgenic techniques for increased milk and meat production.
Similar to Role of Biotecnology in Pharmaceutical Industory (20)
How to Make a Field Mandatory in Odoo 17Celine George
In Odoo, making a field required can be done through both Python code and XML views. When you set the required attribute to True in Python code, it makes the field required across all views where it's used. Conversely, when you set the required attribute in XML views, it makes the field required only in the context of that particular view.
Chapter wise All Notes of First year Basic Civil Engineering.pptxDenish Jangid
Chapter wise All Notes of First year Basic Civil Engineering
Syllabus
Chapter-1
Introduction to objective, scope and outcome the subject
Chapter 2
Introduction: Scope and Specialization of Civil Engineering, Role of civil Engineer in Society, Impact of infrastructural development on economy of country.
Chapter 3
Surveying: Object Principles & Types of Surveying; Site Plans, Plans & Maps; Scales & Unit of different Measurements.
Linear Measurements: Instruments used. Linear Measurement by Tape, Ranging out Survey Lines and overcoming Obstructions; Measurements on sloping ground; Tape corrections, conventional symbols. Angular Measurements: Instruments used; Introduction to Compass Surveying, Bearings and Longitude & Latitude of a Line, Introduction to total station.
Levelling: Instrument used Object of levelling, Methods of levelling in brief, and Contour maps.
Chapter 4
Buildings: Selection of site for Buildings, Layout of Building Plan, Types of buildings, Plinth area, carpet area, floor space index, Introduction to building byelaws, concept of sun light & ventilation. Components of Buildings & their functions, Basic concept of R.C.C., Introduction to types of foundation
Chapter 5
Transportation: Introduction to Transportation Engineering; Traffic and Road Safety: Types and Characteristics of Various Modes of Transportation; Various Road Traffic Signs, Causes of Accidents and Road Safety Measures.
Chapter 6
Environmental Engineering: Environmental Pollution, Environmental Acts and Regulations, Functional Concepts of Ecology, Basics of Species, Biodiversity, Ecosystem, Hydrological Cycle; Chemical Cycles: Carbon, Nitrogen & Phosphorus; Energy Flow in Ecosystems.
Water Pollution: Water Quality standards, Introduction to Treatment & Disposal of Waste Water. Reuse and Saving of Water, Rain Water Harvesting. Solid Waste Management: Classification of Solid Waste, Collection, Transportation and Disposal of Solid. Recycling of Solid Waste: Energy Recovery, Sanitary Landfill, On-Site Sanitation. Air & Noise Pollution: Primary and Secondary air pollutants, Harmful effects of Air Pollution, Control of Air Pollution. . Noise Pollution Harmful Effects of noise pollution, control of noise pollution, Global warming & Climate Change, Ozone depletion, Greenhouse effect
Text Books:
1. Palancharmy, Basic Civil Engineering, McGraw Hill publishers.
2. Satheesh Gopi, Basic Civil Engineering, Pearson Publishers.
3. Ketki Rangwala Dalal, Essentials of Civil Engineering, Charotar Publishing House.
4. BCP, Surveying volume 1
ISO/IEC 27001, ISO/IEC 42001, and GDPR: Best Practices for Implementation and...PECB
Denis is a dynamic and results-driven Chief Information Officer (CIO) with a distinguished career spanning information systems analysis and technical project management. With a proven track record of spearheading the design and delivery of cutting-edge Information Management solutions, he has consistently elevated business operations, streamlined reporting functions, and maximized process efficiency.
Certified as an ISO/IEC 27001: Information Security Management Systems (ISMS) Lead Implementer, Data Protection Officer, and Cyber Risks Analyst, Denis brings a heightened focus on data security, privacy, and cyber resilience to every endeavor.
His expertise extends across a diverse spectrum of reporting, database, and web development applications, underpinned by an exceptional grasp of data storage and virtualization technologies. His proficiency in application testing, database administration, and data cleansing ensures seamless execution of complex projects.
What sets Denis apart is his comprehensive understanding of Business and Systems Analysis technologies, honed through involvement in all phases of the Software Development Lifecycle (SDLC). From meticulous requirements gathering to precise analysis, innovative design, rigorous development, thorough testing, and successful implementation, he has consistently delivered exceptional results.
Throughout his career, he has taken on multifaceted roles, from leading technical project management teams to owning solutions that drive operational excellence. His conscientious and proactive approach is unwavering, whether he is working independently or collaboratively within a team. His ability to connect with colleagues on a personal level underscores his commitment to fostering a harmonious and productive workplace environment.
Date: May 29, 2024
Tags: Information Security, ISO/IEC 27001, ISO/IEC 42001, Artificial Intelligence, GDPR
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This slide is special for master students (MIBS & MIFB) in UUM. Also useful for readers who are interested in the topic of contemporary Islamic banking.
Strategies for Effective Upskilling is a presentation by Chinwendu Peace in a Your Skill Boost Masterclass organisation by the Excellence Foundation for South Sudan on 08th and 09th June 2024 from 1 PM to 3 PM on each day.
LAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UPRAHUL
This Dissertation explores the particular circumstances of Mirzapur, a region located in the
core of India. Mirzapur, with its varied terrains and abundant biodiversity, offers an optimal
environment for investigating the changes in vegetation cover dynamics. Our study utilizes
advanced technologies such as GIS (Geographic Information Systems) and Remote sensing to
analyze the transformations that have taken place over the course of a decade.
The complex relationship between human activities and the environment has been the focus
of extensive research and worry. As the global community grapples with swift urbanization,
population expansion, and economic progress, the effects on natural ecosystems are becoming
more evident. A crucial element of this impact is the alteration of vegetation cover, which plays a
significant role in maintaining the ecological equilibrium of our planet.Land serves as the foundation for all human activities and provides the necessary materials for
these activities. As the most crucial natural resource, its utilization by humans results in different
'Land uses,' which are determined by both human activities and the physical characteristics of the
land.
The utilization of land is impacted by human needs and environmental factors. In countries
like India, rapid population growth and the emphasis on extensive resource exploitation can lead
to significant land degradation, adversely affecting the region's land cover.
Therefore, human intervention has significantly influenced land use patterns over many
centuries, evolving its structure over time and space. In the present era, these changes have
accelerated due to factors such as agriculture and urbanization. Information regarding land use and
cover is essential for various planning and management tasks related to the Earth's surface,
providing crucial environmental data for scientific, resource management, policy purposes, and
diverse human activities.
Accurate understanding of land use and cover is imperative for the development planning
of any area. Consequently, a wide range of professionals, including earth system scientists, land
and water managers, and urban planners, are interested in obtaining data on land use and cover
changes, conversion trends, and other related patterns. The spatial dimensions of land use and
cover support policymakers and scientists in making well-informed decisions, as alterations in
these patterns indicate shifts in economic and social conditions. Monitoring such changes with the
help of Advanced technologies like Remote Sensing and Geographic Information Systems is
crucial for coordinated efforts across different administrative levels. Advanced technologies like
Remote Sensing and Geographic Information Systems
9
Changes in vegetation cover refer to variations in the distribution, composition, and overall
structure of plant communities across different temporal and spatial scales. These changes can
occur natural.
This presentation includes basic of PCOS their pathology and treatment and also Ayurveda correlation of PCOS and Ayurvedic line of treatment mentioned in classics.
How to Fix the Import Error in the Odoo 17Celine George
An import error occurs when a program fails to import a module or library, disrupting its execution. In languages like Python, this issue arises when the specified module cannot be found or accessed, hindering the program's functionality. Resolving import errors is crucial for maintaining smooth software operation and uninterrupted development processes.
Reimagining Your Library Space: How to Increase the Vibes in Your Library No ...Diana Rendina
Librarians are leading the way in creating future-ready citizens – now we need to update our spaces to match. In this session, attendees will get inspiration for transforming their library spaces. You’ll learn how to survey students and patrons, create a focus group, and use design thinking to brainstorm ideas for your space. We’ll discuss budget friendly ways to change your space as well as how to find funding. No matter where you’re at, you’ll find ideas for reimagining your space in this session.
A review of the growth of the Israel Genealogy Research Association Database Collection for the last 12 months. Our collection is now passed the 3 million mark and still growing. See which archives have contributed the most. See the different types of records we have, and which years have had records added. You can also see what we have for the future.
3. INTRODUCTION
• The use of biotechnology in medicine is the most influential developments in the
world of technology in this 21st century.
• To eradicate diseases and maintain a health and vigor, biotechnology has served
human beings a lot.
• Biotechnology is central in almost all the pharmaceutical processes. Modern
biotechnology is often associated with the use of genetically modified
microorganisms such as Escherichia coli or yeast for the production of substances
like antibiotics and synthetic products.
• Certain products from genetically altered mammalian cells, such as Chinese
hamster ovary cells. Another promising new biotechnology application is the
development of plant-made pharmaceuticals.
4. BIOPHARMING
• This term refers to the use of genetic engineering to insert genes that code
for useful pharmaceuticals into host animals or plants that would otherwise
not express those genes, thus creating a genetically modified organism
(GMO).
• For example the drug is called atryn, is an antithrombin protein purified from
the milk of genetically modified goats. Additionally, a most recent treatment
for gaucher’s disease has been approved. This drug is produced in cultured
transgenic carrots and tobacco cells.
5. • SYNTHESIS OF HORMONES
1) HUMAN GROWTH HORMONE
• Production was first done in 1979
• Produced in the pituitary gland at the base of the brain mediating growth.
• Currently used in the treatment of "hypopituitary dwarfism," a children's
disease in which the pituitary malfunctions and secretes insufficient amounts of
(hgh) for normal growth.
• It is synthesized by:
inserting DNA coding for human growth hormone into a plasmid that was
implanted in Escherichia coli bacteria.
6. 2) SYNTHESIS OF INSULIN
• First produced in Escherichia coli
through recombinant DNA
technology in 1978.
• Prior to the development of this
technique, insulin was extracted
from the glands of cattle, pigs, and
other farm animals.
7. 3) ERYTHROPOIETIN
• Boosts production of red blood cells and used to treat anemia, caused by cancer,
chemotherapy, or kidney failure.
• EPO is a complex protein with chains of sugars on its surface. Chinese hamster
ovary cells, a type of mammalian cells often used in biotech manufacturing, are
generally used to produce a humanlike version of EPO for pharmaceutical use.
• EPO must be produced in mammalian cells because microbes like bacteria and
yeast don’t have the cellular machinery to stick the critical sugar chains onto the
protein.
8. • SYNTHESIS OF CLOTTING FACTORS
• Initially, clotting factors produced from donated blood that was partially screened of
HIV.
• Human clotting factor ix was the first to be produced through recombinant DNA
technology using transgenic Chinese hamster ovary cells in 1986. Plasmids containing
the factor IX gene, along with plasmids were inserted into Chinese hamster ovary cells
via transfection.
• As the development in recombinant DNA technology advanced, FDA approved
production human blood clotting factor VIII using transgenic Chinese hamster ovary
cells, the first such blood clotting factor produced using recombinant DNA technology.
9. Cytokines
Hormone like molecules that can control reactions between cells. Activate
immune-system cells such as lymphocytes and macrophages:
1) Interferons
Synthesized in 1980
act against viruses and uncontrolled cell proliferation, leads to cancer. Virtually all
conventional chemotherapeutic agents act directly on cancer cells. When
interferons act on cancer cells, however, they do so indirectly—by affecting the
functioning of the immune system.
10. 2) interleukins
• Interleukins function as messengers between leukocytes.
• Interleukin-2 (il-2) stimulates t lymphocytes.
• The FDA has approved a recombinant variant of IL-2, aldesleukin for treating
renal cell carcinoma.
• Interleukin receptors on astrocyte in the hippocampus are also known to be
involved in the development of spatial memories in mice
11. MONOCLONAL ANTIBODIES (MOABS)
• Share a specific antigenic target are identical.
• products of hybridomas cells that result from the biotech fusion of bone-
marrow tumor cells and b lymphocytes. Hybridomas can be geared to
produce specific moabs continuously.
• Designed for a particular antigen on cancer cells can initiate an immune
response that would destroy cancer cells without harming normal cells.
• FDA has approved the moab drug muromonab-cd3 for the treatment of
immune-system rejection of transplanted hearts, kidneys, and livers.
• Infliximab (ca2), appears effective against crohn’s disease, an immune-
system disorder marked by intestinal inflammation.
Editor's Notes
Goeddel DV, Heyneker HL, Hozumi T, et al. (October 1979). "Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone". Nature. 281 (5732): 544–8
F.M.Steinberg,j.raso(1998). “biotech pharmaceuticals and biotherapy”.USA.(2):50-53
R.J.Kaufman, L.C.Wasley, B.C.Furie,, C.B.Shoemaker (1986). “Expression,purification f recombinant factor in ovary cells” J. Biol. Chem.261 (21): 9622–8
F.M.Steinberg,j.raso(1998). “biotech pharmaceuticals and biotherapy”.USA.(2):50-53
C.Brocker, D. Thompson , A.Matsumoto, D.W.Nebert (2010).”evolutionary divergence of human interleukin gene family” Human Genomics. 5 (1): 30-35
M. Miller(1998) “To build a better mousetrap, use human parts”. JNCI. 90:14–16.