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For the students studying Medical Microbiology like MSC BSC MBBS DENTAL BPTH Nursing DMLT Pharmacy etc and also for those who are preparing for exams such as NEET
Pneumonia Symposia presented at Hôpital Sacré Coeur in Milot, Haiti, 2011.
CRUDEM’s Education Committee (a subcommittee of the Board of Directors) sponsors one-week medical symposia on specific medical topics, i.e. diabetes, infectious disease. The classes are held at Hôpital Sacré Coeur and doctors and nurses come from all over Haiti to attend.
This document summarizes community acquired pneumonia, its types , causes, organisms, CRUB-65 score, difference with atypical pneumonia, investigations, treatment and prevention.
Multipex for viral and atypical pneumoniaPathKind Labs
Diagnosis of pneumonia can be challeging, especially if pathogens other than Streptococcus pneumoniae are involved Multiplex PCR with results available within the same day can investigate the presence or absence of 16 viruses and 5 bacteria, enablng the physician to make informed decisions about treatment, prognosis and public health and infection control measures.
Upper respiratory tract infections are characterized by self-limited irritation and swelling of the upper airways together with a cough that does not indicate pneumonia, does not have a coexisting medical condition that could be the cause of the patient's symptoms, and does not have a history of chronic bronchitis, emphysema, or COPD. Presentation gives an overview on "Upper Respiratory Tract Infections", including causes, symptoms, diagnosis, and Treatment to cure. For more information, please contact us: 9779030507.
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10. Epiglottis
• Edema and inflammation of epiglottis and soft
tissue above vocal cords
• Age: children 2–6 years
• Symptoms: Fever, Difficulty in swallowing,
Inspiratory stridor
• Most common agent: Haemophilus influenzae
type b
13. CURB-65score
- C (Confusion) = 1 point
- U (blood urea nitrogen >19 mg/dL) = 1 point
- R (respiratory rate >30 min) = 1 point
- B (BP <90/60) = 1 point
- 65 (Age ≥65 years) = 1 point
• Higher the score, greater is the mortality
• If the score ≤1, outpatient therapy is indicated
If the score >1, patient should be hospitalized
19. BRONCHITIS
• Inflammation of bronchus, which occurs
either as an extension of upper respiratory
tract infection such as influenza or may be
caused directly by bacterial agents such as
Bordetella.
• Common symptoms - fever, cough, sputum
production, and rarely croup- like features
21. BRONCHIOLITIS
• Inflammation of the smaller airways (bronchioles)
• It presents as an acute viral infection that
primarily occurs in children less than 2 year
• Symptoms: Acute onset of wheeze, dyspnea,
cough, rhinorrhea, and respiratory distress
• Respiratory syncytial viruses account for 40–80%
24. Microscopy
• Albert staining
• Gram staining
• Acid fast staining
• GMS stain
• Immunofluorescence microscopy of
nasopharyngeal aspirate
25.
26. Culture
• For bacteriological culture: Blood agar, chocolate agar and
MacConkey agar
• For isolation of C. diphtheriae: Loeffler’s serum slope and
potassium tellurite agar
• For M. tuberculosis: LJ medium and incubated for up to 6–
8 weeks
• For fungal pathogen isolation: Sabouraud dextrose agar
• Viral - Appropriate cell lines
30. TREATMENT
• Community-acquired pneumonia (CAP)
Empiric regimen is determined by presence of co-
morbidity and prediction of prognosis by CURB-65
scoring system
• CAP, hospitalized (if CURB65 score >1):
- IV ceftriaxone plus azithromycin or
- IV levofloxacin
- Add vancomycin if CA-MRSA suspected
32. Hospital-acquired pneumonia (HAP)
• Empirical therapy: Gram-negative (e.g.
piperacillin-tazobactam or meropenem) +
Gram-positive coverage (e.g. vancomycin)
• Definitive therapy: The empirical treatment
should be tailored based on the organism
isolated and its
Editor's Notes
Symptoms:
Pharynx and/or tonsils become inflamed, red, swollen, and show exudate, and sometimes a membrane is formed
Viruses: (most common cause) Influenza virus, Parainfluenza virus, Coxsackievirus A, Rhinovirus, Coronavirus, Epstein-Barr virus, Adenoviruses
Bacteria: Streptococcus pyogenes (most common bacterial cause), Streptococcus groups C and G, Arcanobacterium species, Corynebacterium diphtheriae and C. ulcerans, Mycoplasma pneumoniae
Vincent angina - Treponema vincentii & Leptotrichia buccalis
Fungal: Candida albicans
Non-productive, harsh, barking cough
Agents: followed by Mycoplasma pneumoniae
CURB65 scoring system - To predict prognosis of CAP
Score >1 patient should be hospitalized
Else the treatment can be given on outpatient basis
No co-morbidity:
Streptococcus pneumoniae (most common)
Atypical pathogens:
Chlamydophila pneumoniae and C. psittaci
Legionella and Mycoplasma
Coxiella burnetii (Q fever)
Viral pneumonia (influenza, adenovirus, parainfluenza, RSV)
Associated with Co-morbidity:
Alcoholism: S. pneumoniae, H. influenzae
COPD: H. influenzae, M. catarrhalis, S. pneumoniae
Post-CVA-aspiration: S. pneumoniae
Post-obstruction of bronchi: pneumoniae, anaerobes
Post-influenza: S. pneumoniae, S.aureus
Hospitalized patients have increased risk of developing pneumonia; most of which are ventilator-associated pneumonia
VAP can be clinically diagnosed by Clinical Pulmonary Infection (CPIS)
Likelihood of VAP is higher when total CPIS is >6
SCORE:0 NO CORRELATION BETWEEN MICROSCOPY AND CULTURE
SCORE:2 POSITIVE CORRELATION BETWEEN MICROSCOPY AND CULTURE
Gram-negative bacilli (most common)
MDR non-fermenters (Pseudomonas & Acinetobacter)
MDR Enterobacteriaceae (E. coli, Klebsiella & Enterobacter)
Staphylococcus aureus (both MRSA and MSSA)
S. pneumoniae (rarely, in early stage)
Influenza, adenovirus, parainfluenza, RSV
Fever, chills, chest pain and cough
Based on area of lungs involved, and type of cough produced
Lobar pneumonia infecting lung parenchyma (alveoli)
Consolidation and productive cough with purulent sputum
- Mostly caused by pyogenic organisms :
Pneumococcus
Haemophilus influenzae
Staphylococcus aureus
Gram-negative bacilli.
Infection occurs in interstitial space of lungs
Cough is characteristically non-productive
Caused by :
Chlamydophila pneumoniae
Mycoplasma pneumoniae
Viral pneumonia
Legionella species
Throat swab: Two swabs should be collected, one for direct examination, other one for culture
A part of the membrane, if present
Nasopharyngeal aspirate for viral diagnosis or for B.pertussis
Albert staining - metachromatic granules in the ends of the bacilli of C. diphtheriae
Gram staining
Detect the quality of the sputum
Pus cells >25/low power field and epithelial cells <5/low power field good quality sputum
Acid fast staining - M. tuberculosis
GMS stain - Pneumocystis jirovecii
Immunofluorescence microscopy of nasopharyngeal aspirate
BARTLETT CRITERIA, MURRAY WASHINGTON CRITERIA
Streptococcus pneumoniae
Pus cells >25/LPF and epithelial cells <10/LPF gram-positive cocci in pair, lanceolate shaped
Alfa hemolytic, draughtsman-shaped colonies on blood agar Sensitive to optochin
Bile soluble, ferments inulin
Haemophilus influenzae
Pus cells >25/LPF and epithelial cells <10/LPF Pleomorphic gram-negative bacilli
Satellitism on blood agar with S. aureus streak line
Staphylococcus aureus
Pus cells >25/LPF and epithelial cells <10/LPF gram-positive cocci in clusters
BA- golden yellow hemolytic colonies Catalase positive, coagulase positive
Gram-negative bacilli
E. coli, Klebsiella, Pseudomonas, etc.)
Pus cells >25/LPF and epithelial cells <10/LPF gram-negative bacilli
Identification is based on:
Growth on MacConkey agar (LF or NLF colonies) and
Biochemical reactions (ICUT: indole, citrate, urease, TSI)
Chlamydophila pneumoniae
Direct immunofluorescence test
Antigen detection by enzyme immunoassay Nucleic acid amplification test (NAAT) detecting specific genes
Serology-antibody detection by
- CFT using LPS antigen
- ELISA using recombinant LPS antigen
- Micro-IF test using outer membrane protein antigen
Legionella pneumophila
Pus cells >25/LPF and epithelial cells <10/LPF Detection of specific antigen in sputum, urine
Growth on BCYE medium
Mycoplasma pneumoniae
Direct immunofluorescence test
Capture ELISA-detecting antigen (P1 adhesin) PCR targeting P1 adhesin gene
Culture-fried egg colonies on PPLO agar Antibody detection
- Non-specific test (cold agglutination test)
Specific test (e.g. ELISA)
Viral pneumonia
Detection of specific viral antigen in sputum Detection of specific viral genes in sputum (PCR)