Viral vectors have been widely used as gene delivery vehicles. Compared to the conventional transfection methods, transduction usually results in much higher gene delivery efficiency, sometimes almost 100%. These are especially useful for gene delivery into primary cells or stem cells which is recalcitrant to conventional transfection methodologies. With years of experience, Creative Biogene has launched a powerful QVirusTM Platform for Lentivirus, Retrovirus, Adeno-associated Virus (AAV), Adenoviruses, Retrovirus, Oncolytic virus, etc. https://qvirus.creative-biogene.com/

1. Our platform offers a comprehensive portfolio
of services for the efficient design, development,
manufacturing and analytical testing of virus vector products.
QVirusTM Platform
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2. An Overview of QVirusTM Platform
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Viral vectors have been widely used as gene delivery vehicles. Compared to the
conventional transfection methods, transduction usually results in much higher gene
delivery efficiency, sometimes almost 100%. However, construction of a viral vector
is a laborious process, and most commercial services in this area have so far been
either too expensive or not user-friendly. With years of experience, Creative Biogene
has launched a powerful QVirusTM Platform for Lentivirus, Adeno-associated Virus
(AAV), Adenoviruses, Retrovirus, Oncolytic virus, etc.

3. QVirusTM Platform
—— Working Principle
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In order to produce viruses with alternate (non-
virus-producing) genomes, naturally occurring
viral genomes have been adapted into a
plasmid-based technology, such that plasmids
can be used to create viruses with specific
genomes (Figure. 1A). That is to say, instead
of a virus infecting a host and giving rise to
more viruses, researchers can introduce
plasmids to a host to generate virus. Moreover,
these plasmids can be modified to give rise to
viral genomes of choice (Figure. 1B). Therefore,
through standard plasmid cloning, viruses can
be engineered to harbor a lot of viral genomes,
enabling researchers to direct genetic functions
widely in cells. Figure 1. Using viral plasmids to generate virus.

4. QVirusTM Platform
—— Multiple Viral Vectors Available
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There are various types of viruses which are commonly used for research, each of
which exhibits different properties, and therefore, are suited for specific research goals.
For instance, AAV is typically preferred for in vivo studies owing to its low
immunogenicity. Different types of viruses also vary in the composition of their viral
genomes, with some having an RNA genome while others have a DNA genome.
Furthermore, some viruses function by permanently integrating into the host's genome
while other viruses are temporary in the host.
Our QVirusTM Platform has developed a number of viruses, including lentivirus, AAV,
adenovirus and retrovirus, etc.

5. QVirusTM Platform
—— Multiple Viral Vectors Available
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The charateristics of different viruses as follows:

6. QVirusTM Platform
—— Viral Vector Construction
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Our QVirusTM Platform offers a variety of optimized viral vectors for your applications.
We can subclone your gene of interest into the viral vectors with custom choices of
promoters, tags, fusions, and selectable markers under different regulatory elements.

7. QVirusTM Platform
—— Virus Packaging
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Viral vectors are one of the most efficient methods of gene delivery to mammalian cells both
in vitro and in vivo. Through years of experience with viral vectors, our QVirusTM Platform
has developed its own proprietary virus packaging systems and efficient protocols for the
rapid generation of pseudoviral particles. The viral expression constructs packaged into
pseudoviral particles can be transduced into cells with very high efficiency, approaching
100% in some cell types. Packaged viral constructs can be transduced into even the most
difficult to transfect cells, such as primary, stem, and differentiated cells with high efficiency.

8. QVirusTM Platform
—— Virus Packaging
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Our QVirusTM Platform offers packaging services for the following virus types:

9. QVirusTM Platform
—— Virus Purification and Titration
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The highly purified virus is a requirement for a lot of downstream applications since
cellular debris and proteins derived from culture media in crude virus preparation
can be toxic to target cells and can cause immunogenic reactions when used for in
vivo injection. Using purified virus stocks also prevents pseudo-transduction, in
which high levels of recombinant protein in the crude supernatant are passively
transferred to target cells during infection.
With years of experience in research of virus, Creative Biogene is able to produce
high purity and high titer virus that is suitable for both in vitro cell experiments and
in vivo animal tests. Our QVirusTM Platform can help you to remove cellular
contaminants, including uncharacterized infection and transduction inhibitors,
residual plasmid DNA, cellular and serum proteins, and highly immunogenic viral
proteins, nucleic acids, and virus fragments.

10. QVirusTM Platform
—— Application
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Viral vectors are tools commonly used by molecular biologists to deliver genetic material into cells.

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QVirusTM Platform
—— Advantages

12. THANK YOU
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