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GPCR Stable Cell Lines
Creative Biogene specializes in developing stable cell lines for G protein-coupled receptors (GPCRs), which play critical roles in various physiological processes and diseases. The company utilizes advanced methods to monitor GPCR signaling and internalization using fluorescent and chemiluminescent assays. With years of experience and a dedicated team, Creative Biogene offers flexible solutions tailored to clients' needs in GPCR-related drug discovery.
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GPCR Stable Cell Lines
- 1. Email: info@creative-biogene.com Website: http://www.creative-biogene.com GPCRStable Cell Line Development Creative Biogene
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- 3. G protein–coupled receptors(GPCRs) are a large family of seven transmembrane protein receptors found in eukaryotes. Coupling with G proteins, these receptors are activated by binding to diverse extracellular ligands including light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters etc. The activated GPCRs further induce intracellular signal transduction pathways and, ultimately, cellular responses. GPCRs are involved in a wide variety of physiological processes and many diseases. To date, many medicinal drugs are GPCR- targeting, and there is an increasing interest in the study of discovering novel GPCR-related drug candidates. Background
- 4. With years ofexperience and thousands of accomplished projects, Creative Biogene offers flexible GPCR detection solutions which can help customers to achieve an ideal GPCR stable cell line suitable for downstream cell- based assay. Leading Capability Engineered cell lines stably overexpressing GPCRs are useful models for detection of GPCR signaling. Creative Biogene owns a team of experienced GPCR biologists working in the area of GPCR biology and drug discovery. We have generated a wide variety of GPCR stable cells with more under development.
- 5. 01 02 03 SecondMessenger-Mediated Pathway β-arrestin Recruitment Pathway GPCR Internalization Technologies & Methods
- 6. Second Messenger-Mediated Pathway GPCRactivation can be monitored by second messengers such as cAMP and calcium. Upon treatment of ligands, the intracellular cAMP or calcium is changed in a GPCR stable cell line which can be detected using cAMP or calcium cell assay kits. To further simplify and visualize the change of second messengers induced by GPCR activities, Creative Biogene has developed a type of stable cell line containing both a GPCR protein and a fluoresent-based reporter system. Ligand induced GPCR modulation can be measured through fluorescence intensity readout. Plate cells Incubate with ligand Detect cAMP or calcium 500000 400000 300000 200000 100000 90 70 50 30 10 -5 -4 -3 -2 -1 0 1 2 3 -5 -4 -3 -2 -1 0 1 2 3
- 7. β-arrestin Recruitment Pathway β-arrestinis a key protein involved in GPCR signal transduction as a negative regulator. GPCR activation stimulates the β-arrestin to translocate and interact with the activated receptor. This interaction leads to the attenuation of GPCR signaling. The ligand-induced β-arrestin activities have been utilized by our scientists for GPCR stable cell line development. In these cell lines, ligand binding triggers β-arrestin recruitment which can be measured through fluoresent or chemiluminescent output.
- 8. GPCR Internalization GPCR internalizationis a process of endocytosis induced by ligand binding. Creative Biogene can generate stable cell lines for monitoring ligand-induced GPCR internalization. By fusing a fluorescent protein to the GPCR or based on enzyme fragment complementation, the receptor internalization can be detected by fluoresent or chemiluminescent imaging. Plate cells Treat with ligand Read fluorescence
- 9. Investigation of GPCR signaling pathways Screeningof GPCR agonists and antagonists High throughput screening and compound profiling Applications
- 10. Highlights Competitive price andquick turnaround time. Easy detection via commercial assay kits, fluoresent or chemiluminescent imaging. Stability through 10 passages, multiplex assays in single cell line. Flexibility in cell types such as HEK293, CHO-K1, U-2 OS, 1321N1 etc. Covering a large broad of GPCR targets from human, mouse, rats and other species. Highlights
- 11. Based on manyyears' experience on cell line development, Creative Biogene's cell culture scientists focus on optimizing all parameters specific to your cell lines to efficiently produce stable cell lines according to your requirements. Our state-of-the-art labs are staffed by some of the best trained and most experienced subject matter experts in the field. Contact us today for a free consultation with the scientific team and discover how Creative Biogene can be a valuable resource and partner for your organization.