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circRNA Service
Creative Biogene is a global biotechnology company that offers high-quality circular RNA (circRNA) services to enhance biomarker discovery and drug development research. Their services include circRNA sequencing, microarray analysis, and bioinformatic support, with a focus on understanding circRNA's role as a miRNA sponge and in various biological functions. The company emphasizes that their analyses are for research use only and not for diagnostic procedures.
circRNA Service
- 1. 1-631-626-9181 info@creative-biogene.com 45-1 Ramsey Road,Shirley, NY 11967, USA circRNA Service Improving the human condition through biotechnology Bioinformatic analysis circRNA-miRNA interaction Functional analysis www.creative-biogene.com circRNA sequencing circRNA microarray www.creative-biogene.com
- 2. Bioinformatic analysis For ResearchUse Only. Not For Use In Diagnostic Procedures. C 2010 Creative Biogene Inc. All Rights Reserved https://www.creative-biogene.com/services/circrna-service.html circRNA Service Improving the human condition through biotechnology Introduction Creative Biogene is a global biotechnology company devoting to offer high-quality service for customers. Our unparalleled circRNA service will accelerate your biomarker discovery and drug development research. Circular RNA (circRNA) is an emerging class of widespread and abundant noncoding RNA. Most circRNAs are conserved cross species and exhibit cell/tissue-specific property. circRNA is a promising biomarker for diagnosis, prognosis and therapeutic response. In addition, circRNA usually functions as a miRNA sponge to suppress the activity of miRNA, suggesting that circRNA is important target molecule in cancer. Service circRNA Screening RNA-seq Microarray Bioinformatic analysis circRNA inhibition circRNA overexpression in vivo in vitro circRNA Functional Analysis Expression Analysis Cellular level Tissue level Clinical samples Mechanism Analysis circRNA-miRNA/RBP RNA immunoprecipitation RNA pull-down Luciferase reporter assay RT-PCR Sanger sequencing Northern blot RNA-FISH Identification and location 1 2 3 4 5 • circRNA SequencingService • circRNA Functional Analysis Service • circRNA Micoarray Service • circRNA-miRNA Interaction AnalysisService Technique route
- 3. Bioinformatic analysis For ResearchUse Only. Not For Use In Diagnostic Procedures. C 2010 Creative Biogene Inc. All Rights Reserved https://www.creative-biogene.com/services/circrna-service.html circRNA Service Improving the human condition through biotechnology Details Application Biomarker Discovery Molecular Diagnosis Drug Development Mechanism Research RNaseR Digestion circRNA Enrichment Strand-Specific Library Preparation Sequencing circRNA Library HiSeq 2500/4000 Data Analysis Bioinformatic Analysis Total RNA circRNA rRNA linear RNA Sample requirements: • Without degradation or DNA contamination • Total RNA amount ≥ 2 μg • OD260/280 > 1.8, OD260/230 > 1.0 • RIN value ≥ 7.0 • Sample concentration ≥ 100 ng/µl Strategies: • circRNA sequencing rRNA depletion: circRNA+miRNA+lncRNA rRNA depletion & RNaseR digestion: circRNA • whole transcriptome resequencing (mRNA+miRNA+lncRNA+circRNA) Recommended sequencing depth: • 150 bp paired end • >40 million reads Bioinformatics Analysis • circRNA identification and annotation • Statistics of different circRNA types • circRNA localization on genome • Expression pattern analysis of circRNA • Differential circRNA expression analysis • Enrichment analysis including GO and KEGG • Target miRNA prediction • circRNA-miRNA/RBP interaction analysis Workflow circRNA Sequencing Service
- 4. Bioinformatic analysis For ResearchUse Only. Not For Use In Diagnostic Procedures. C 2010 Creative Biogene Inc. All Rights Reserved https://www.creative-biogene.com/services/circrna-service.html circRNA Service Improving the human condition through biotechnology circRNA Sequencing Service Advantages Bioinformic Analysis Case Numbers of circRNAs Genomic origin Clustered heatmap Differential expression analysis Multidimensional scaling screen Interaction network CONSULTING SEQUENCING ANALYSIS RESULTS DG REPORTS
- 5. Bioinformatic analysis For ResearchUse Only. Not For Use In Diagnostic Procedures. C 2010 Creative Biogene Inc. All Rights Reserved https://www.creative-biogene.com/services/circrna-service.html circRNA Service Improving the human condition through biotechnology circRNA Microarray Service Workflow Microarray Formats DetectedcircRNAs Database Human circRNAArray-V2.0 8 x 15K 13600 circBase Mouse circRNAArray-2.0 8 x 15K 14200 circBase Rat circRNAArray-2.0 8 x 15K 14100 circBase Human circRNAMicroarray-V2.0* 4 x 180K 170340 circBase • Excellent sensitivity and dynamic range • Head-to-tail junction specific probes • Efficient circRNA labeling • Accurate and reliable circRNA expression profiling • In depth data analysis for establishing circRNA-miRNA/protein interaction network Creative Biogene utilizes unique head-to-tail junction specific probes, combining with enzymatic linear RNA removal, to enrich, capture and quantify circRNA targets at high sensitivity and specificity. Our circRNA microarray can be widely used in human, rat and mouse. Total RNA isolation RNA QC Linear RNA and rRNA removal cDNA synthesis Data extraction and analysis Array scanning Array hybridization and washing Labeling Microarray list Benefits
- 6. Bioinformatic analysis For ResearchUse Only. Not For Use In Diagnostic Procedures. C 2010 Creative Biogene Inc. All Rights Reserved https://www.creative-biogene.com/services/circrna-service.html circRNA Service Improving the human condition through biotechnology After obtaining candidate circRNA through RNA-seq, microarray and bioinformatics analysis,it is necessary to confirm its localization and manipulate its expression to further elucidate the biological function of circRNA. Service Advantages • Accurate subcellular localization of circRNA • Efficient overexpression system: the expression level is hundreds of times higher than control • Specifically reduce the expression of circRNA and do not affect the host linear RNA expression • Quick turnaround time • Cost-effective circRNA-FISH service • high sensitivity • bright fluorescent signal. circRNA silencing service: • high efficiency • high stability • RT-qPCR service • Northern blot service circRNA overexpression service • high specificity circRNA Functional Analysis circRNA-FISH RT-qPC circRNA overexpression vector
- 7. Bioinformatic analysis For ResearchUse Only. Not For Use In Diagnostic Procedures. C 2010 Creative Biogene Inc. All Rights Reserved https://www.creative-biogene.com/services/circrna-service.html circRNA Service Improving the human condition through biotechnology circRNA-miRNA/RBP Interaction Analysis Service Functions of circRNA In general, circRNA functions as a miRNA sponge to suppress the activity of miRNA, suggesting that circRNA is important target molecule in cancer. Besides, circRNA also functions as RNA banding protein (RBP) sponge or transcription/translation regulator involved in a variety of biological processes. Service Luciferase reporter assay • Validating the sponge function of miRNA • Detecting target miRNA RNA immunoprecipitation (RIP) RNA pull-down assay Creative Biogene utilizes several methods to explore the detailed binding sites for circRNA- mi RNA and circ RNA- RBP ( RNA binding protein) interactions, including RNA immunoprecipitation (RIP) combined with luciferase reporter assay, RIP followed by sequencing, and RNA pull down combined with mass spectrometry. On this basis, Creative Biogene is a reliable partner that can work with you to accelerate the speed to achieve your research goals. 1-631-626-9181 info@creative-biogene.com 45-1 Ramsey Road, Shirley, NY 11967, USA