Abstract: Musa paradisiaca often refereed as Banana is an ancient herbaceous flowering plant and the most earlier crop to be cultivated. It is one the important component of diet for all classes of people worldwide. All the parts of the plant is believed to have beneficial effects in different ailments and an excellent nutritional element. The present study was aimed to find the protective effect of Musa paradisiacal fruit extract on acute pancreatitis in rats.
Male Sprague dawley rats were randomly divided in to 4 groups. Control group with normal saline; disease control group where acute pancreatitis was induced by using a single dose of L-arginine (2.5g/kg b.w); Musa paradisiacal fruit extract were prophylactically administered before the induction of acute pancreatitis at a dose of 200 and 400 mg/kg b.w orally in normal saline for 7 days. At the end of the study, blood samples and isolated pancreas were subjected to different pancreatic, antioxidant and inflammatory biomarker analysis. The remaining tissue was subjected to histopathological studies and DNA fragmentation assay for assessing the damage and protection of the tissues.
The results of the study revealed that prophylactic administration of the extracts reduced the amylase and lipase levels when compared to disease control group as well as improved the overall antioxidant status in a dose dependent manner. Further, the extracts also showed a protective effect against apoptosis.
In conclusion, the present study suggest that administration of Musa paradisiacal fruit extract exhibited a protective effect on acute pancreatitis and further investigation is required for its molecular based mechanisms.
Keywords: Musa paradisiacal, acute pancreatitis, free radicals, apoptosis.
Hepatoprotective Activity of Methanolic Extract of Whole Plant of Pulicaria W...IOSRJPBS
Natural remedies from medicinal plants are considered to be effective and safe alternative treatment for liver injury. The present study was conducted to evaluate the hepatoprotective activity of methanolic extract of whole plant of Pulicaria wightiana in wistar rats. The studies were conducted using the two popular inducing agents Paracetamol (2 g/kg, p.o.) in 1% NaCMC and Carbon tetrachloride (1 ml/kg). Silymarin (100 mg/kg, p.o.) was used as reference drug in the respective models. The effect was estimated by measuring the enzymatic levels and histo- pathological studies. The methanolic extract of whole plant of Pulicaria wightiana has shown very significant hepatoprotection against both Paracetamol and CCl4 - induced hepatotoxicity study models in wistar rats. This was evidenced by marked reduction in marker enzymes in serum. Histopathological studies also confirmed the hepatoprotective nature of the extract
Hepatoprotective Activity of Cinnamon Zeylanicum Leaves against Alcohol Induc...IJERA Editor
Plants play an important role in the life of human, as the major source of food, as well as for the maintenance and improvement of health and for the elimination of the enemies since ages. Plants are the basic source of knowledge of modern medicine. The present study was conducted to evaluate the hepatoprotective activity of aqueos extract of aerial parts of Cinnamon zeylanicum are evaluated in alcohol induced hepatotoxicity in albino rats. Silymarin (100mg/kg) was given as reference standard. The aqueos extract of aerial parts of Cinnamon zeylanicum have shown very significant hepatoprotection against alcohol induced hepatotoxicity in albino rats in reducing SGOT, SGPT, Alkaline phosphatase (ALP) and GGT and levels of total bilirubin and total protein were investigated and showed an increase in alcohol induced rats when compared to control. The extracts of the test plant exhibited significant (p < 0.05) hepatoprotective activity against the alcohol induced liver models by improving liver function which was indicated by reduction in the levels of SGOT, SGPT, ALP, GGT, total bilirubin and total protein.
Background: Dennentia tripetalla (Pepper Fruit) belongs to the Annonaceae family and is abundant in Nigeria. Its
fruit in folklore medicine is used for treatment of varying ailments. While ample research evidence exists on the
plants fruit and seed, no current study exists on the toxicological profile of the plant leaves.
Methods: qualitative and quantitative phytochemicals and In vitro antioxidant assays were carried out using
standard methods. The acute toxicity study indicates that the LD50 was higher than 2000 mg/Kg body weight. Subchronic
toxicity studies was carried out using five groups of rats. Group 1 served as control, 2–5 received 100 mg/
Kg, 200 mg/Kg, 500 mg/Kg and 1000 mg/Kg body weight orally for 28 days.
Results: Post-administration biochemical analysis indicates there was increased weight in rats administered 100
mg/kg and 200 mg/kg while it reduced in the 500 mg/kg group. Significant elevations of liver function markers
were reported for 200 mg/kg and 500 mg/kg respectively. Serum and hepatic protein profiles remained unaltered.
Renal function analysis revealed elevated serum urea and creatinine for 200 and 500 mg/kg groups, elevated serum
Na+ and Ca+ and reduced serum Cl− for the 500 mg/Kg group. Elevated Kidney K+ and Ca+ levels, reduced Cl−
were significantly observed in 500 mg/Kg group. Significant rise in hepatic and renal lipid peroxidation was
observed in 200 and 500 mg/Kg groups. There were observed disarmament of the antioxidant defense systems
occasioned by rise and drop in tissue (hepatic, renal, testes, heart) Superoxide dismutase (SOD), Catalase (Cat),
Glutathione-s-transferase (GST), Glutathione peroxidase (GPx) activities in the test groups relative to control.
Histopathological examination indicated architectural aberrations at 500 and 1000 mg/kg.
Conclusions: It concluded that the plant had significant phytochemical and antioxidant properties of medical
interest and possessed toxic properties in rats when administered at a dose above 200 mg/Kg over a prolonged
period of time.
The Detection of Testicular Protein Expression of Rats (Rattus norvegicus) af...AI Publications
One of the testes' primary functions is to produce the haploid germ cells needed for sexual reproduction. Good vitality can be achieved through a healthy biological pattern and require lasting persistence throughout a productive male's life. This study aims to detect protein expression in rats' testes after being treated with ethanol Fenugreek seeds. As many as 32 male rats (Rattus norvegicus), aged 2-3 months (250-300 g) without anatomical abnormalities were used as experimental animals. They were divided into four groups: P0 as a control group was treated with normal drinking water, while groups of P1 to P3 were given 500, 1000, and 1500 mg kg-1 body weight/day of ethanol extract from Fenugreek seeds. The experiment of testicular protein expression was conducted with Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). The testes data were analyzed by One-way Analysis of Variance (ANOVA) and the Duncan Mean Range Test. The study concluded that Fenugreek's effect is believed to have an inducing impression on protein synthesis in the cells making up the testes. Besides, body weight, body weight gain, testicular weight, and testicular protein showed no significant difference (P> 0.05).
The Detection of Testicular Protein Expression of Rats (Rattus norvegicus) af...AI Publications
One of the testes' primary functions is to produce the haploid germ cells needed for sexual reproduction. Good vitality can be achieved through a healthy biological pattern and require lasting persistence throughout a productive male's life. This study aims to detect protein expression in rats' testes after being treated with ethanol Fenugreek seeds. As many as 32 male rats (Rattus norvegicus), aged 2-3 months (250-300 g) without anatomical abnormalities were used as experimental animals. They were divided into four groups: P0 as a control group was treated with normal drinking water, while groups of P1 to P3 were given 500, 1000, and 1500 mg kg-1 body weight/day of ethanol extract from Fenugreek seeds. The experiment of testicular protein expression was conducted with Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). The testes data were analyzed by One-way Analysis of Variance (ANOVA) and the Duncan Mean Range Test. The study concluded that Fenugreek's effect is believed to have an inducing impression on protein synthesis in the cells making up the testes. Besides, body weight, body weight gain, testicular weight, and testicular protein showed no significant difference (P> 0.05).
Hepatoprotective Activity of Methanolic Extract of Whole Plant of Pulicaria W...IOSRJPBS
Natural remedies from medicinal plants are considered to be effective and safe alternative treatment for liver injury. The present study was conducted to evaluate the hepatoprotective activity of methanolic extract of whole plant of Pulicaria wightiana in wistar rats. The studies were conducted using the two popular inducing agents Paracetamol (2 g/kg, p.o.) in 1% NaCMC and Carbon tetrachloride (1 ml/kg). Silymarin (100 mg/kg, p.o.) was used as reference drug in the respective models. The effect was estimated by measuring the enzymatic levels and histo- pathological studies. The methanolic extract of whole plant of Pulicaria wightiana has shown very significant hepatoprotection against both Paracetamol and CCl4 - induced hepatotoxicity study models in wistar rats. This was evidenced by marked reduction in marker enzymes in serum. Histopathological studies also confirmed the hepatoprotective nature of the extract
Hepatoprotective Activity of Cinnamon Zeylanicum Leaves against Alcohol Induc...IJERA Editor
Plants play an important role in the life of human, as the major source of food, as well as for the maintenance and improvement of health and for the elimination of the enemies since ages. Plants are the basic source of knowledge of modern medicine. The present study was conducted to evaluate the hepatoprotective activity of aqueos extract of aerial parts of Cinnamon zeylanicum are evaluated in alcohol induced hepatotoxicity in albino rats. Silymarin (100mg/kg) was given as reference standard. The aqueos extract of aerial parts of Cinnamon zeylanicum have shown very significant hepatoprotection against alcohol induced hepatotoxicity in albino rats in reducing SGOT, SGPT, Alkaline phosphatase (ALP) and GGT and levels of total bilirubin and total protein were investigated and showed an increase in alcohol induced rats when compared to control. The extracts of the test plant exhibited significant (p < 0.05) hepatoprotective activity against the alcohol induced liver models by improving liver function which was indicated by reduction in the levels of SGOT, SGPT, ALP, GGT, total bilirubin and total protein.
Background: Dennentia tripetalla (Pepper Fruit) belongs to the Annonaceae family and is abundant in Nigeria. Its
fruit in folklore medicine is used for treatment of varying ailments. While ample research evidence exists on the
plants fruit and seed, no current study exists on the toxicological profile of the plant leaves.
Methods: qualitative and quantitative phytochemicals and In vitro antioxidant assays were carried out using
standard methods. The acute toxicity study indicates that the LD50 was higher than 2000 mg/Kg body weight. Subchronic
toxicity studies was carried out using five groups of rats. Group 1 served as control, 2–5 received 100 mg/
Kg, 200 mg/Kg, 500 mg/Kg and 1000 mg/Kg body weight orally for 28 days.
Results: Post-administration biochemical analysis indicates there was increased weight in rats administered 100
mg/kg and 200 mg/kg while it reduced in the 500 mg/kg group. Significant elevations of liver function markers
were reported for 200 mg/kg and 500 mg/kg respectively. Serum and hepatic protein profiles remained unaltered.
Renal function analysis revealed elevated serum urea and creatinine for 200 and 500 mg/kg groups, elevated serum
Na+ and Ca+ and reduced serum Cl− for the 500 mg/Kg group. Elevated Kidney K+ and Ca+ levels, reduced Cl−
were significantly observed in 500 mg/Kg group. Significant rise in hepatic and renal lipid peroxidation was
observed in 200 and 500 mg/Kg groups. There were observed disarmament of the antioxidant defense systems
occasioned by rise and drop in tissue (hepatic, renal, testes, heart) Superoxide dismutase (SOD), Catalase (Cat),
Glutathione-s-transferase (GST), Glutathione peroxidase (GPx) activities in the test groups relative to control.
Histopathological examination indicated architectural aberrations at 500 and 1000 mg/kg.
Conclusions: It concluded that the plant had significant phytochemical and antioxidant properties of medical
interest and possessed toxic properties in rats when administered at a dose above 200 mg/Kg over a prolonged
period of time.
The Detection of Testicular Protein Expression of Rats (Rattus norvegicus) af...AI Publications
One of the testes' primary functions is to produce the haploid germ cells needed for sexual reproduction. Good vitality can be achieved through a healthy biological pattern and require lasting persistence throughout a productive male's life. This study aims to detect protein expression in rats' testes after being treated with ethanol Fenugreek seeds. As many as 32 male rats (Rattus norvegicus), aged 2-3 months (250-300 g) without anatomical abnormalities were used as experimental animals. They were divided into four groups: P0 as a control group was treated with normal drinking water, while groups of P1 to P3 were given 500, 1000, and 1500 mg kg-1 body weight/day of ethanol extract from Fenugreek seeds. The experiment of testicular protein expression was conducted with Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). The testes data were analyzed by One-way Analysis of Variance (ANOVA) and the Duncan Mean Range Test. The study concluded that Fenugreek's effect is believed to have an inducing impression on protein synthesis in the cells making up the testes. Besides, body weight, body weight gain, testicular weight, and testicular protein showed no significant difference (P> 0.05).
The Detection of Testicular Protein Expression of Rats (Rattus norvegicus) af...AI Publications
One of the testes' primary functions is to produce the haploid germ cells needed for sexual reproduction. Good vitality can be achieved through a healthy biological pattern and require lasting persistence throughout a productive male's life. This study aims to detect protein expression in rats' testes after being treated with ethanol Fenugreek seeds. As many as 32 male rats (Rattus norvegicus), aged 2-3 months (250-300 g) without anatomical abnormalities were used as experimental animals. They were divided into four groups: P0 as a control group was treated with normal drinking water, while groups of P1 to P3 were given 500, 1000, and 1500 mg kg-1 body weight/day of ethanol extract from Fenugreek seeds. The experiment of testicular protein expression was conducted with Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). The testes data were analyzed by One-way Analysis of Variance (ANOVA) and the Duncan Mean Range Test. The study concluded that Fenugreek's effect is believed to have an inducing impression on protein synthesis in the cells making up the testes. Besides, body weight, body weight gain, testicular weight, and testicular protein showed no significant difference (P> 0.05).
Phytochemical Analysis and Antibacterial Activity of Psidium Guajava against ...ijtsrd
The some plants have been good source of drugs for microbial resistance. The research for newer source of antibiotics is a global challenges in pharmaceutical companies. Present time plants are being extensively explored for harboring medicinal properties. Phytochemical are naturally occurring compounds present in the medicinal plants such as flavonoids, steroids, alkaloids and phenolic compounds. Psidium guajava is a phototherapeutic plant used in folk medicine and is believed to have active components that helps in treatment and management of various disease such as wounds, ulcers, etc. Guava extract has exhibited remarkable antimicrobial activity against microorganisms. This study investigates the phytochemical and antibacterial properties of Psidium guajava leaf extracts. Antibacterial activity at the plant extract were determined against K. pneumoniae, S. aureus, E. coli. Psidium guajava leaf extracts provided scientific evidence for the rational use of P. juajava leaves in prevention of disorders due to Presence of some useful phytochemicals and in the treatment of disease caused by some bacterial pathogens such as S. aureus, B. cereus, K. pneumonae, E. coli. The present work demonstrates the antimicrobial potential of Psidium guajava leaves extracts by using various solvents. Pooja Soudawat | Anand Verma "Phytochemical Analysis and Antibacterial Activity of Psidium Guajava against Some Clinical Pathogens" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-6 , October 2019, URL: https://www.ijtsrd.com/papers/ijtsrd29134.pdf Paper URL: https://www.ijtsrd.com/biological-science/biotechnology/29134/phytochemical-analysis-and-antibacterial-activity-of-psidium-guajava-against-some-clinical-pathogens/pooja-soudawat
Screening and evaluation of medicinal properties of grape fruit varieties in ...EditorIJAERD
Theni districts could be considered one of the highest grapes producing area also export best quality of
Paneer dhrakshai Indian table grapes in Tamil Nadu. The research work focused to identify the nutritive compounds in
the grapes (Vitis vinifera) species which are a wonderful fruit having pulp content, vibrant color and exert an immense
health benefits. Grape fruits were found to be rich source of Anthocyanin content and vitamin C also macromolecules
contents such as carbohydrate contents of 29mg, Protein 18.7mg and fat content 3.7mg /100 g of f. wt. Micronutrients
were estimated by AAS which showed that calcium content was 3.1mg/g.f.wt, iron content 2.7 mg/g.f.wt, potassium 2.6
mg/g.f.wt found to be more than other minerals in fresh extracts of grape fruits. The comparative results of revealed
considerable amount of phytoconstituents in grape fruits in which anthocyanins alone has been taken to evaluate
therapeutic effects on breast cancer cell MCF-7 cell viability test was carried out to determine cell proliferation. The
results revealed presence of anthocyanins in high quantity in the process of purification through various biochemical
methods that could ultimately be used for preventing cancerous cell proliferation which reduces the development of
cancer diseases. On comparison Grapes (Vitis vinifera) may serve as functional food with vital pharmaceutical and
biological value.
Present study deals with the investigation of hepatoprotective activity of Tephrosia purpurea Linn stem. Powdered stem was extracted with methanol and subjected for the preliminary phytochemical screening. Acute toxicity study of the extract was carried out following OECD guidelines 423 and found safe upto the dose 2000 mg/kg, p.o. Hepatoprotective activity of extract was evaluated against CCl induced hepatotoxicity in Wistar albino rats. Rats were divided into five groups containing 4 6 mice per group. Group 1 animals were administered with vehicle only, Group II animals were administered with CCl (1.4 ml/kg p.o.) to induced hepatotoxicity, 4 group III animals were administered with silymarin (25 mg/kg) for 7 days and CCl (1.4 ml/kg p.o.) on fifth day, group IV and V animals were administered with 4 methanol extract of T. purpurea stem at 75 and 150 mg/kg, po respectively for 7 days and CCl (1.4 ml/kg p.o.) on fifth day of treatment schedule. Biochemical 4 parameters (SGPT, SGOT, ALP, total bilirubin and direct bilirubin) were assessed in all the experimental animals. Phytochemical investigation of methanol extract of T. purpurea stem revealed the presence of flavanoids, phytosterols, alkaloids and proteins. Methanol extract of T. purpurea stem was exhibited dose dependant hepatoprotective activity comparable to that of silymarin.
Biochemical effect of Aqueous Carica papaya Seed and Leaf Extracts on Serum B...iosrjce
The cost of administering modern anti diabetic drugs is usually beyond the reach of most people in
the low income group especially those in the developing world where the disease is on the increase. This has led
to the current shift to the use of preparations from various parts of plants because of the current increase in the
knowledge of their toxicity, side effects, active constituents and doses. This study was conducted to compare the
effects of the aqueous Carica papaya seed and leaf extracts on serum biochemistry of alloxan induced diabetic
rats especially glucose level. Male Wister rats weighing 150-200g were induced with single freshly prepared
alloxan monohydrate (150 mg/kg body weight).Diabetes was confirmed after seven days in alloxan-induced rats
showing fasting blood glucose levels ≥ 200mg/dl. The diabetic rats were randomly allocated into three
experimental groups which received Seed extract, leaf extract or normal saline depending on the group. The
extracts were administered orally for twenty-eight days after which the animals were sacrificed and blood
samples were collected for Biochemical analyses. The results showed that both extracts have significant
hypoglycaemic, hepatoprotective and nephroprotective effects although extract of the seed proved to be more
potent than that of the leaf
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
Preliminary phytochemical studies and evaluation of Antipyretic property of t...researchplantsciences
Purpose-The root of Ritchiea longipedicellata was claimed to have anti pyretic, analgesic and antimicrobial properties. The people of Idemili area in Anambra State of Nigeria use the decoction of it to treat wounds, running stomach, aches and pains as well as fever. It is to this backdrop that this investigation was carried out to ascertain the veracity of the claim.
Methodology-The root of Ritchiea longipedicellata was collected and dried at ambient temperature. It was pulverized into powder. 500 gm of the powdered drug was placed into a two litre beaker containing one litre of methanol. It was allowed to stand with occasional shaking for 48 h. The content was filtered and the filtrate was concentrated using rotary evaporator. The extract contains the following secondary metabolites-alkaloids, flavonoids, terpenoids, saponins and glycosides. The antipyretic activity was investigated using brewer’s yeast to induce pyrexia.
Result -The root of Ritchiea longipedicellata exhibited significant (p<0.05) antipyretic effect at 600 mg/kg from 1 h to 4 h and at 3 h to 4 h at 300 mg/kg compared with the control.
Conclusion-The claim of Idemili people of Anambra State Nigeria on the use of Ritchiea longipedicellata appears to be obvious in line with the results of the investigation.
Article Citation:
Chinedu Fred Anowi, Ezugwu CO, Ilodigwe EE, Ajaghaku DL.
Preliminary Phytochemical Studies and Evaluation of Antipyretic Property of the Methanol Extract of the Rootbark of Ritchiea longipedicellata Gilg (capparidaceae)
Journal of Research in Plant Sciences (2013) 2(2): 192-195.
Full Text:
http://plantsciences.co.in/documents/PS0048.pdf
Cytotoxicity of Blended Versus Single Medicinal Mushroom Extracts on Human Ca...Jolene1981
ABSTRACT: The use of mushrooms contributes to human nutrition by providing low lipid content of lipids and high dietary fiber content, as well as significant content of other biologically active compounds such as polysaccharides, minerals, vitamins, and polyphenolic antioxidants. This study aimed to determine the content of polyphenols and polysaccharides, as well as the cytotoxic and antioxidative properties of several medicinal mushroom preparations. The content of total phenols and flavonoids of preparations of blended mushroom extracts (Lentifom, Super Polyporin, Agarikon, Agarikon Plus, Agarikon.1, and Mykoprotect.1) was evaluated quantitatively by using ultraviolet–visible spectroscopy spectrophotometric methods. The antioxidant capacity of the preparations was evaluated using the ABTS (2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) and ferric reducing/antioxidant power assays. The content of water-soluble polysaccharides was determined using a specific gravimetric method, based on ethanol precipitation. To determine cytotoxic effects of single and blended mushroom extracts, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and neutral red assays were conducted using human small cell lung cancer, lung adenocarcinoma, colon cancer, and brain astrocytoma cancer cells. The obtained results suggest that due to the significant content of beneficial polyphenolic antioxidants and soluble polysaccharides, use of these mushroom preparations is beneficial in maintaining good health, as well as in the prevention and adjuvant biotherapy of various human pathological aberrations. These results reveal that these extracts exhibit different cytotoxic effects on tumor cells originating from different tissues. In addition, the comparison of investigated blended mushroom extracts with three well-known commercial mushroom products derived from single mushroom species or single mushroom compounds shows that blended mushroom extracts exhibit significantly stronger cytotoxic effects on human tumor cell lines.
Preliminary phytochemical analysis and in vivo evaluation of antipyretic effe...CrimsonAlternativemedicine
Preliminary phytochemical analysis and in vivo evaluation of antipyretic effects of methanolic extract of Argyreia pilosa Wight & Arn. by DSNBK Prasanth in Advances in Complementary & Alternative Medicine
The antipyrexia action of the methanol extract of A. pilosa had been explored utilizing the yeast evoked pyrexia procedure in rabbits. Paracetamol utilized as a positive control as well as negative control group acquired distilled water. Rectal temperatures of all rabbits had been documented instantly prior to the administration of the extract or vehicle or paracetamol as well as again at 30min period for 3h utilizing digital thermometer. The extract had been additionally phytochemically tested with regard to alkaloids, tannins, saponins, flavonoids, cardiac glycosides and phenols. At 400mg/kg dosage the extract revealed considerable decrease in yeast evoked raised temperature when compared with that of standard drug paracetamol where by the extract dose 200mg/kg had been less effective as compared to higher dose (p<0.05). Phytochemical testing demonstrated the existence of flavonoids, alkaloids, tannins, phenols, steroids, acid compounds, glycosides, amino acids, and proteins. This research confirmed that this methanol extract of A. pilosa at a dose of 400mg/kg owns considerable antipyretic outcome against the yeast-induced raised temperature. The antipyretic activity of A. pilosaextract could be due to its secondary metabolites, which probably consist of flavonoids like Rutin and Quercetin; sterols like β-Sitosterol. But, further phytochemical, as well as biological tests, are recommended to determine the other active chemical constituents accountable for the antipyretic activity.
Effect of lyophilized aqueous leaf extract of Aquilaria subintegra on aphrodi...Mohammed Muayad TA
Objective: To investigate the effects of Aquilaria subintegra leaf aqueous extracts on the
aphrodisiac properties including sexual behaviour, testosterone level, percentage of pregnancy,
number of offspring and male to female ratio of offspring in ICR mice.
Methods: In this experiment, each male cohabitated with one female in a polysulfone cage.
30 ICR male mice were divided into 6 groups that received normal saline (the control group),
50 mg/kg, 100 mg/kg, 200 mg/kg, 500 mg/kg, and 1 000 mg/kg body weight of Aquilaria
subintegra leaf aqueous extracts orally for 21 days consecutively. Sexual behavior, percentage
of pregnancy, number of offspring and male to female ratio of offspring in ICR mice were
measured according to the established methods. Testosterone level was measured by using
enzyme-linked immunosorbent assay.
Results: Mice that received Aquilaria subintegra leaf aqueous extracts at 50 mg/kg body
weight (day 0) had significantly higher mount frequency as compared to the control group;
groups treated with 100, 500, 1 000 mg/kg body weight extracts produced a greater number
of offsprings when compared to the control group. All aphrodisiac parameters were similar
between the treatment groups and the control group, indicating that Aquilaria subintegra leaf
aqueous extract did not significantly alter the aphrodisiac parameters.
Conclusions: Aquilaria subintegra leaf aqueous extracts have no effect on the aphrodisiac
properties, but could increase the breeding rate in mice.
EVALUATION OF ANALGESIC AND ANTI-INFLAMMATORY.pdfgynomark
Amaranthus roxburghianus is one of the traditionally well-known plants with outstanding therapeutic properties, and
is used mostly in treating different diseases in India. Thus, based on these medicinal properties, various investigations
have been undertaken in order to appraise the pharmacological activities and the chemical composition of these
species. Here, we elucidate the analgesic and anti-inflammatory activity of Amaranthus roxburghianus ethanolic
leaves extract. phytochemical screening of Amaranthus roxburghianus extract showed the presence of alkaloids,
Carbohydrates, Glycosides, Flavonoids, Tannins, Proteins, Amino Acids. the ethanolic leaves extract of Amaranthus
roxburghianus, possess peripheral and central analgesic activity in animal model. The Amaranthus roxburghianus
leaves extract shows anti-inflammatory activity in different animal model. Flavonoids and tannins are the major
constituents of Amaranthus roxburghianus leaves, which may be responsible for its Analgesic, Anti-inflammatory
activity.
KEYWORDS: Amaranthus roxburghianus, Phytochemical screening, Analgesic activity, Anti-inflammatory activity
Polygonum Persicaria (Linn.) and its Active Principle have a hepatoprotective...AI Publications
The aim of this analysis was to see whether the aqueous extract of the roots of Polygonum persicaria (PP) and its active principle, Tannic Acid (TA), had a hepatoprotective and antioxidant effect in rats provided Carbon tetrachloride (1.5 ml/kg, i.p). Twenty albino wistar rats were divided into five groups: control, CCl4-induced hepatotoxicity, hepatotoxicity with Polygonum persicaria and Tannic acid, and a normal group given 100 mg/kg silymarin. After 14 days, the rats were sacrificed. Toxicity testing was carried out on 12 rats. They were randomly allocated to one of three groups: control, Polygonum persicaria 200 mg/kg (B.wt), and Tannic acid 200 mg/kg (B.wt). The amounts of liver homogenate enzymes (glutathione peroxidase, glutathione-S-transferase, glucose-6-phosphatase dehydrogenase, and glutathione reductase enzymes) were greatly restored by extracts of PP and TA at the tested concentrations, supporting the biochemical results. Tannic acid, in contrast to Polygonum persicaria, tends to have a greater liver defensive role toward carbon tetrachloride-induced hepatotoxicity, as well as antioxidant properties and mild anticancer activity against cell viability at higher concentrations. The histological alterations in the liver indicated the injury. Polygonum persicaria & its active principle Tannic acid has strong antioxidant properties as well as hepatoprotective effects against CCl4-induced hepatotoxicity, as demonstrated by these observations.
Anti-anemic Activity Of Polygonum Glabrum.pdfgynomark
In present research work, anti-anemic effect of methanolic extract (ME) of aerial parts of polygonum glabrum (PG) was studied in phenylhydrazine (PHZ) induced anemic rats. Fifteen albino wistar rats (3 rats in each group) weighing 100-150 grams were randomly distributed into: normal control group, anemic control group, standard reference group administered with vitamin B12 syrup and two test control groups administered with 200mg/kg and 400mg/kg of ME of aerial part of PG respectively twice daily. ME of PG was given orally for one month for test groups and collect blood samples from all groups. Collected blood samples were estimated for complete blood count includes RBC, Hb, WBC, PCV, MCH, Platelets, MCV and MCHC. It was observed that, test control group with 400mg/kg of PG dose improves the Hb percentage in PHZ induced anemic rats very similar to standard reference group. It concludes the anti-anemic effect of phenolics and flavonoids of PG in PHZ induced anemic rats.
Key words: Anti-anemic, Methanolic, Polygonum glabrum, Phenylhydrazine.
Evaluation of Anti Inflammatory and Behavioural Activities of Calycophyllum S...gynomark
The Herb Calycophyllum spruceanum (family:Rubiaceae) known to have several medicinal properties. Methanolic extract of bark was administered to albino rats to study the anti-inflammatory activity by carrageenan induced models as well as behavioural activity by using actophotometer. The powdered bark of Calycophyllmm spruceanum was extracted using a Soxhlet apparatus with methanol. The obtained extract was assessed for anti-inflammatory activity at 125, 250 and 500mg/kg/b.w. dose using diclofenac sodium as a standard drug and for the behavioural activity of Calycophyllmm spruceanum at a dose of 125, 260 and 500mg/kg/b.w using diazepam as the standard drug. The obtained results were compared with those of the standard. There results of anti-inflammatory activity revealed that the extract exhibits significant reduction of paw volume in comparison with the control group For the behavioural activity, the extract exhibited significant decrease in the mean score which indicated reduction in aggression in comparison with the control group. Considerable depressant anxiolytic effects in association with anti-inflammatory activity as demonstrated in the present study might balance each present other, and thus, the plant would be utilized in different inflammation and excitatory situations.
More Related Content
Similar to Protective Effect of Musa paradisiaca Fruit.pdf
Phytochemical Analysis and Antibacterial Activity of Psidium Guajava against ...ijtsrd
The some plants have been good source of drugs for microbial resistance. The research for newer source of antibiotics is a global challenges in pharmaceutical companies. Present time plants are being extensively explored for harboring medicinal properties. Phytochemical are naturally occurring compounds present in the medicinal plants such as flavonoids, steroids, alkaloids and phenolic compounds. Psidium guajava is a phototherapeutic plant used in folk medicine and is believed to have active components that helps in treatment and management of various disease such as wounds, ulcers, etc. Guava extract has exhibited remarkable antimicrobial activity against microorganisms. This study investigates the phytochemical and antibacterial properties of Psidium guajava leaf extracts. Antibacterial activity at the plant extract were determined against K. pneumoniae, S. aureus, E. coli. Psidium guajava leaf extracts provided scientific evidence for the rational use of P. juajava leaves in prevention of disorders due to Presence of some useful phytochemicals and in the treatment of disease caused by some bacterial pathogens such as S. aureus, B. cereus, K. pneumonae, E. coli. The present work demonstrates the antimicrobial potential of Psidium guajava leaves extracts by using various solvents. Pooja Soudawat | Anand Verma "Phytochemical Analysis and Antibacterial Activity of Psidium Guajava against Some Clinical Pathogens" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-6 , October 2019, URL: https://www.ijtsrd.com/papers/ijtsrd29134.pdf Paper URL: https://www.ijtsrd.com/biological-science/biotechnology/29134/phytochemical-analysis-and-antibacterial-activity-of-psidium-guajava-against-some-clinical-pathogens/pooja-soudawat
Screening and evaluation of medicinal properties of grape fruit varieties in ...EditorIJAERD
Theni districts could be considered one of the highest grapes producing area also export best quality of
Paneer dhrakshai Indian table grapes in Tamil Nadu. The research work focused to identify the nutritive compounds in
the grapes (Vitis vinifera) species which are a wonderful fruit having pulp content, vibrant color and exert an immense
health benefits. Grape fruits were found to be rich source of Anthocyanin content and vitamin C also macromolecules
contents such as carbohydrate contents of 29mg, Protein 18.7mg and fat content 3.7mg /100 g of f. wt. Micronutrients
were estimated by AAS which showed that calcium content was 3.1mg/g.f.wt, iron content 2.7 mg/g.f.wt, potassium 2.6
mg/g.f.wt found to be more than other minerals in fresh extracts of grape fruits. The comparative results of revealed
considerable amount of phytoconstituents in grape fruits in which anthocyanins alone has been taken to evaluate
therapeutic effects on breast cancer cell MCF-7 cell viability test was carried out to determine cell proliferation. The
results revealed presence of anthocyanins in high quantity in the process of purification through various biochemical
methods that could ultimately be used for preventing cancerous cell proliferation which reduces the development of
cancer diseases. On comparison Grapes (Vitis vinifera) may serve as functional food with vital pharmaceutical and
biological value.
Present study deals with the investigation of hepatoprotective activity of Tephrosia purpurea Linn stem. Powdered stem was extracted with methanol and subjected for the preliminary phytochemical screening. Acute toxicity study of the extract was carried out following OECD guidelines 423 and found safe upto the dose 2000 mg/kg, p.o. Hepatoprotective activity of extract was evaluated against CCl induced hepatotoxicity in Wistar albino rats. Rats were divided into five groups containing 4 6 mice per group. Group 1 animals were administered with vehicle only, Group II animals were administered with CCl (1.4 ml/kg p.o.) to induced hepatotoxicity, 4 group III animals were administered with silymarin (25 mg/kg) for 7 days and CCl (1.4 ml/kg p.o.) on fifth day, group IV and V animals were administered with 4 methanol extract of T. purpurea stem at 75 and 150 mg/kg, po respectively for 7 days and CCl (1.4 ml/kg p.o.) on fifth day of treatment schedule. Biochemical 4 parameters (SGPT, SGOT, ALP, total bilirubin and direct bilirubin) were assessed in all the experimental animals. Phytochemical investigation of methanol extract of T. purpurea stem revealed the presence of flavanoids, phytosterols, alkaloids and proteins. Methanol extract of T. purpurea stem was exhibited dose dependant hepatoprotective activity comparable to that of silymarin.
Biochemical effect of Aqueous Carica papaya Seed and Leaf Extracts on Serum B...iosrjce
The cost of administering modern anti diabetic drugs is usually beyond the reach of most people in
the low income group especially those in the developing world where the disease is on the increase. This has led
to the current shift to the use of preparations from various parts of plants because of the current increase in the
knowledge of their toxicity, side effects, active constituents and doses. This study was conducted to compare the
effects of the aqueous Carica papaya seed and leaf extracts on serum biochemistry of alloxan induced diabetic
rats especially glucose level. Male Wister rats weighing 150-200g were induced with single freshly prepared
alloxan monohydrate (150 mg/kg body weight).Diabetes was confirmed after seven days in alloxan-induced rats
showing fasting blood glucose levels ≥ 200mg/dl. The diabetic rats were randomly allocated into three
experimental groups which received Seed extract, leaf extract or normal saline depending on the group. The
extracts were administered orally for twenty-eight days after which the animals were sacrificed and blood
samples were collected for Biochemical analyses. The results showed that both extracts have significant
hypoglycaemic, hepatoprotective and nephroprotective effects although extract of the seed proved to be more
potent than that of the leaf
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
Preliminary phytochemical studies and evaluation of Antipyretic property of t...researchplantsciences
Purpose-The root of Ritchiea longipedicellata was claimed to have anti pyretic, analgesic and antimicrobial properties. The people of Idemili area in Anambra State of Nigeria use the decoction of it to treat wounds, running stomach, aches and pains as well as fever. It is to this backdrop that this investigation was carried out to ascertain the veracity of the claim.
Methodology-The root of Ritchiea longipedicellata was collected and dried at ambient temperature. It was pulverized into powder. 500 gm of the powdered drug was placed into a two litre beaker containing one litre of methanol. It was allowed to stand with occasional shaking for 48 h. The content was filtered and the filtrate was concentrated using rotary evaporator. The extract contains the following secondary metabolites-alkaloids, flavonoids, terpenoids, saponins and glycosides. The antipyretic activity was investigated using brewer’s yeast to induce pyrexia.
Result -The root of Ritchiea longipedicellata exhibited significant (p<0.05) antipyretic effect at 600 mg/kg from 1 h to 4 h and at 3 h to 4 h at 300 mg/kg compared with the control.
Conclusion-The claim of Idemili people of Anambra State Nigeria on the use of Ritchiea longipedicellata appears to be obvious in line with the results of the investigation.
Article Citation:
Chinedu Fred Anowi, Ezugwu CO, Ilodigwe EE, Ajaghaku DL.
Preliminary Phytochemical Studies and Evaluation of Antipyretic Property of the Methanol Extract of the Rootbark of Ritchiea longipedicellata Gilg (capparidaceae)
Journal of Research in Plant Sciences (2013) 2(2): 192-195.
Full Text:
http://plantsciences.co.in/documents/PS0048.pdf
Cytotoxicity of Blended Versus Single Medicinal Mushroom Extracts on Human Ca...Jolene1981
ABSTRACT: The use of mushrooms contributes to human nutrition by providing low lipid content of lipids and high dietary fiber content, as well as significant content of other biologically active compounds such as polysaccharides, minerals, vitamins, and polyphenolic antioxidants. This study aimed to determine the content of polyphenols and polysaccharides, as well as the cytotoxic and antioxidative properties of several medicinal mushroom preparations. The content of total phenols and flavonoids of preparations of blended mushroom extracts (Lentifom, Super Polyporin, Agarikon, Agarikon Plus, Agarikon.1, and Mykoprotect.1) was evaluated quantitatively by using ultraviolet–visible spectroscopy spectrophotometric methods. The antioxidant capacity of the preparations was evaluated using the ABTS (2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) and ferric reducing/antioxidant power assays. The content of water-soluble polysaccharides was determined using a specific gravimetric method, based on ethanol precipitation. To determine cytotoxic effects of single and blended mushroom extracts, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and neutral red assays were conducted using human small cell lung cancer, lung adenocarcinoma, colon cancer, and brain astrocytoma cancer cells. The obtained results suggest that due to the significant content of beneficial polyphenolic antioxidants and soluble polysaccharides, use of these mushroom preparations is beneficial in maintaining good health, as well as in the prevention and adjuvant biotherapy of various human pathological aberrations. These results reveal that these extracts exhibit different cytotoxic effects on tumor cells originating from different tissues. In addition, the comparison of investigated blended mushroom extracts with three well-known commercial mushroom products derived from single mushroom species or single mushroom compounds shows that blended mushroom extracts exhibit significantly stronger cytotoxic effects on human tumor cell lines.
Preliminary phytochemical analysis and in vivo evaluation of antipyretic effe...CrimsonAlternativemedicine
Preliminary phytochemical analysis and in vivo evaluation of antipyretic effects of methanolic extract of Argyreia pilosa Wight & Arn. by DSNBK Prasanth in Advances in Complementary & Alternative Medicine
The antipyrexia action of the methanol extract of A. pilosa had been explored utilizing the yeast evoked pyrexia procedure in rabbits. Paracetamol utilized as a positive control as well as negative control group acquired distilled water. Rectal temperatures of all rabbits had been documented instantly prior to the administration of the extract or vehicle or paracetamol as well as again at 30min period for 3h utilizing digital thermometer. The extract had been additionally phytochemically tested with regard to alkaloids, tannins, saponins, flavonoids, cardiac glycosides and phenols. At 400mg/kg dosage the extract revealed considerable decrease in yeast evoked raised temperature when compared with that of standard drug paracetamol where by the extract dose 200mg/kg had been less effective as compared to higher dose (p<0.05). Phytochemical testing demonstrated the existence of flavonoids, alkaloids, tannins, phenols, steroids, acid compounds, glycosides, amino acids, and proteins. This research confirmed that this methanol extract of A. pilosa at a dose of 400mg/kg owns considerable antipyretic outcome against the yeast-induced raised temperature. The antipyretic activity of A. pilosaextract could be due to its secondary metabolites, which probably consist of flavonoids like Rutin and Quercetin; sterols like β-Sitosterol. But, further phytochemical, as well as biological tests, are recommended to determine the other active chemical constituents accountable for the antipyretic activity.
Effect of lyophilized aqueous leaf extract of Aquilaria subintegra on aphrodi...Mohammed Muayad TA
Objective: To investigate the effects of Aquilaria subintegra leaf aqueous extracts on the
aphrodisiac properties including sexual behaviour, testosterone level, percentage of pregnancy,
number of offspring and male to female ratio of offspring in ICR mice.
Methods: In this experiment, each male cohabitated with one female in a polysulfone cage.
30 ICR male mice were divided into 6 groups that received normal saline (the control group),
50 mg/kg, 100 mg/kg, 200 mg/kg, 500 mg/kg, and 1 000 mg/kg body weight of Aquilaria
subintegra leaf aqueous extracts orally for 21 days consecutively. Sexual behavior, percentage
of pregnancy, number of offspring and male to female ratio of offspring in ICR mice were
measured according to the established methods. Testosterone level was measured by using
enzyme-linked immunosorbent assay.
Results: Mice that received Aquilaria subintegra leaf aqueous extracts at 50 mg/kg body
weight (day 0) had significantly higher mount frequency as compared to the control group;
groups treated with 100, 500, 1 000 mg/kg body weight extracts produced a greater number
of offsprings when compared to the control group. All aphrodisiac parameters were similar
between the treatment groups and the control group, indicating that Aquilaria subintegra leaf
aqueous extract did not significantly alter the aphrodisiac parameters.
Conclusions: Aquilaria subintegra leaf aqueous extracts have no effect on the aphrodisiac
properties, but could increase the breeding rate in mice.
EVALUATION OF ANALGESIC AND ANTI-INFLAMMATORY.pdfgynomark
Amaranthus roxburghianus is one of the traditionally well-known plants with outstanding therapeutic properties, and
is used mostly in treating different diseases in India. Thus, based on these medicinal properties, various investigations
have been undertaken in order to appraise the pharmacological activities and the chemical composition of these
species. Here, we elucidate the analgesic and anti-inflammatory activity of Amaranthus roxburghianus ethanolic
leaves extract. phytochemical screening of Amaranthus roxburghianus extract showed the presence of alkaloids,
Carbohydrates, Glycosides, Flavonoids, Tannins, Proteins, Amino Acids. the ethanolic leaves extract of Amaranthus
roxburghianus, possess peripheral and central analgesic activity in animal model. The Amaranthus roxburghianus
leaves extract shows anti-inflammatory activity in different animal model. Flavonoids and tannins are the major
constituents of Amaranthus roxburghianus leaves, which may be responsible for its Analgesic, Anti-inflammatory
activity.
KEYWORDS: Amaranthus roxburghianus, Phytochemical screening, Analgesic activity, Anti-inflammatory activity
Polygonum Persicaria (Linn.) and its Active Principle have a hepatoprotective...AI Publications
The aim of this analysis was to see whether the aqueous extract of the roots of Polygonum persicaria (PP) and its active principle, Tannic Acid (TA), had a hepatoprotective and antioxidant effect in rats provided Carbon tetrachloride (1.5 ml/kg, i.p). Twenty albino wistar rats were divided into five groups: control, CCl4-induced hepatotoxicity, hepatotoxicity with Polygonum persicaria and Tannic acid, and a normal group given 100 mg/kg silymarin. After 14 days, the rats were sacrificed. Toxicity testing was carried out on 12 rats. They were randomly allocated to one of three groups: control, Polygonum persicaria 200 mg/kg (B.wt), and Tannic acid 200 mg/kg (B.wt). The amounts of liver homogenate enzymes (glutathione peroxidase, glutathione-S-transferase, glucose-6-phosphatase dehydrogenase, and glutathione reductase enzymes) were greatly restored by extracts of PP and TA at the tested concentrations, supporting the biochemical results. Tannic acid, in contrast to Polygonum persicaria, tends to have a greater liver defensive role toward carbon tetrachloride-induced hepatotoxicity, as well as antioxidant properties and mild anticancer activity against cell viability at higher concentrations. The histological alterations in the liver indicated the injury. Polygonum persicaria & its active principle Tannic acid has strong antioxidant properties as well as hepatoprotective effects against CCl4-induced hepatotoxicity, as demonstrated by these observations.
Anti-anemic Activity Of Polygonum Glabrum.pdfgynomark
In present research work, anti-anemic effect of methanolic extract (ME) of aerial parts of polygonum glabrum (PG) was studied in phenylhydrazine (PHZ) induced anemic rats. Fifteen albino wistar rats (3 rats in each group) weighing 100-150 grams were randomly distributed into: normal control group, anemic control group, standard reference group administered with vitamin B12 syrup and two test control groups administered with 200mg/kg and 400mg/kg of ME of aerial part of PG respectively twice daily. ME of PG was given orally for one month for test groups and collect blood samples from all groups. Collected blood samples were estimated for complete blood count includes RBC, Hb, WBC, PCV, MCH, Platelets, MCV and MCHC. It was observed that, test control group with 400mg/kg of PG dose improves the Hb percentage in PHZ induced anemic rats very similar to standard reference group. It concludes the anti-anemic effect of phenolics and flavonoids of PG in PHZ induced anemic rats.
Key words: Anti-anemic, Methanolic, Polygonum glabrum, Phenylhydrazine.
Evaluation of Anti Inflammatory and Behavioural Activities of Calycophyllum S...gynomark
The Herb Calycophyllum spruceanum (family:Rubiaceae) known to have several medicinal properties. Methanolic extract of bark was administered to albino rats to study the anti-inflammatory activity by carrageenan induced models as well as behavioural activity by using actophotometer. The powdered bark of Calycophyllmm spruceanum was extracted using a Soxhlet apparatus with methanol. The obtained extract was assessed for anti-inflammatory activity at 125, 250 and 500mg/kg/b.w. dose using diclofenac sodium as a standard drug and for the behavioural activity of Calycophyllmm spruceanum at a dose of 125, 260 and 500mg/kg/b.w using diazepam as the standard drug. The obtained results were compared with those of the standard. There results of anti-inflammatory activity revealed that the extract exhibits significant reduction of paw volume in comparison with the control group For the behavioural activity, the extract exhibited significant decrease in the mean score which indicated reduction in aggression in comparison with the control group. Considerable depressant anxiolytic effects in association with anti-inflammatory activity as demonstrated in the present study might balance each present other, and thus, the plant would be utilized in different inflammation and excitatory situations.
Evaluation of Antiulcer Activity of Extract of Calycophyllum Spruceanum (Bent...gynomark
Objective The objective of the study was to evaluate the antiulcer activity of ethanolic extract of Calycophyllum spruceanum bark. Methodology The ethanol-HCL and pylorus ligation induced models was selected using swiss albino mice and the animals were fasted overnight and then grouped as negative control (0.9% normal saline), standard (omeprazole 30mg/kg), test 1 (CSBE 100mg/kg) and test 2 (CSBE 200mg/kg). The ulcer score, ulcer index and percent ulcer inhibition were determined in ethanol/HCL model. The volume of gastric secretion, pH, total acidity, ulcer index and percent of ulcer inhibition was determined in pylorus ligation model. The ulcerogenic abrasions in the mucosal membrane were observed under a microscope (10X) and scored manually. Results The preliminary phytochemical screening indicated the presence of sterols, alkaloids, glycosides, flavonoids, saponins and coumarins. The bark extract was tolerable at dose of 2000mg/kg. The selected doses of the extracts of 100, 200mg/kg was shown the significant and dose dependent antiulcer activity in ethanol/HCL and pylorus-ligation models as 11.08 %, 32.59% and 28.29%, 37.03% respectively. The dose of 200mg/kg of CSBE was shown the superlative activity ((#p?0.001)) in both models. Conclusion: It was concluded that, the ethanolic extract of Calycophyllum spruceanum bark has a significant antiulcer activity. This activity might be due to presence of supportive phytochemical like flavonoids, alkaloids, steroids. and glycosides. Hence, further evaluation is needed to isolate and identify the specific constituents responsible for the activity.
Evaluation of Antihyperlipidemic activity of Calycophyllum Spruceanum.pdfgynomark
Introduction:Hyperlipidemia is a metabolic disorder characterized by fluctuated blood lipid
levels which can lead to several diseases.Learning is the ability to acquire new information and
skills through experience whereas Memory is a process by which information acquired through
learning is stored and retrieved.
Objective:To evaluate the antihyperlipidemic activity and also nootropic activity of methanolic
extract of Calycophyllum spruceanumbark
Materialsand methods:Screening of methanolic extract of Calycophyllum spruceanum bark
(MECSB)for antihyperlipidemic activity was done in high-fat diet Induced rats using atorvastatin
as standard and extracts at a dose of 125, 250, and 500mg/kg. To screen nootropic activity rats
were pretrained using Cook’s Pole Climbing Apparatus.scopolamine was used to induce amnesia
in rats and piracetam as standard and extracts at a dose of 125, 250, and 500mg/kg.
Results: Significantdecrease (p <0.5) in cholesterol, triglycerides, LDL, VLDL, and increase in
HDL levels shown that the extract has Antihyperlipidemic activity. An increase in CAR
(Conditioned Avoidance Response) and less time taken by the animal as jump response to avoid
shock shows that the extract has Nootropic Activity.
Key Words – Hyperlipidemia, Nootropic activity,Calycophyllum spruceanum, Cholesterol, pole climbing apparatus
EVALUATION OF ANTI HYPERLIPIDEMIC ACTIVITY OF ETHANOLIC EXTRACT OF AMARANTHUS...gynomark
Hyperlipidemia is a disorder of lipid metabolism manifested by increase of plasma concentrations of the various lipid and lipoprotein fractions such as increase of serum total cholesterol (TC), low-density lipoprotein (LDL), triglyceride (TG) concentrations, and a decrease in the high-density lipoprotein (HDL) concentration. The aim of the present study is to promote a potential new herbal formulation to prevent atherosclerosis at a low cost. In this study ethanolic extract of Amaranthus roxbhurgianus- showed positive to following phytochemical constituent’s Carbohydrates, Glycosides, Flavonoids, Proteins, Sterols. The significant reduction in serum cholesterol,TG,LDL, ,AI, and also increased in total HDL level in the different dose level(100,200 mg/kg) in high cholesterol diet. Hence it is going to be concluded that the potential benefits of the extracts of Amaranthus roxbhurgianus has been demonstrated well in advance and can be used further to demonstrate the antihyperlipidemic as well as controlling of both triglyceride levels and reducing the risk of factors of cholesterol inducers. The aforementioned results of the research suggest that the Amaranthus roxbhurgianus found to have the potential antihyperlipidemic action.
Keywords: Anti-hyperlipidemic activity, Amaranthus Roxbhurgianus, Albino rats
Thus the results of the present investigation clearly indicated that the selected medicinal plants possess good antihyperlipidemic activity in atherogenic diet induced hyperlipidemic rats and led to the development of new Herbal formulation possessing antihyperlipidemic and antiatherosclerotic activities.
Hence it is going to be concluded that the potential benefits of the extracts of Amaranthus roxbhurgianus has been demonstrated well in advance and can be used further to demonstrate the antihyperlipidemic as well as controlling of both triglyceride levels and reducing the risk of factors of cholesterol inducers. The aforementioned results of the research suggest that the Amaranthus roxbhurgianus found to have the potential antihyperlipidemic action.
The results found are encouraging for further studies on the selected plants and to identify the bioactive compounds.
EVALUATION OF NEPHROPROTECTIVE ACTIVITY OF MORNIDO CITROFOLIA IN ALBINO RATS.pdfgynomark
Nephroprotective agents are material that has potential to minimize the effects of nephrotoxic agents. Medicinal
plants have curative properties due to the presence of various complex chemical substances. Morinda citrifolia L
(Noni) also known as Indian mulberry is a common plant known to grow in the tropical countries. The present study
was focused on the investigation of Nephroprotective activity of the ethanolic extract of fruit Juice of Morinda
citrifolia on cisplatin induced nephrotoxicity in Wistar albino rats. The phytochemical investigation revealed the
presence of carbohydrate, alkaloids, flavanoids, glycosides, saponins, tannins, phenols and anthroquinone in
EEMC. The administration of cisplatin during experimentation is effectively induced apoptosis and necrosis, which
was similar to acute renal failure in human. Therefore,it is an effective and an ideal model for nephrotoxicity
research. The evaluation of renal parameters on nephrotoxic rats with EEMC showed significantly elevate the
attenuated body weight, urine volume, creatinine clearanceand significantly reduce in elevated serum creatinine
level, which supports its Nephroprotective activity.
Key words: Nephroprotective activity, Mornido citrofolia, Albino rats, phytochemical screening
Evaluation of Antiurolithiatic Activity of Canthium dicoccum Ethanolic Extrac...gynomark
Objective:
The aim of the present study was to evaluate the effect of ethanolic extract of Canthium dicoccum on
Gentamicin and Caliculi Producing Diet (CPD) induced urolithasis in rats.
Methods:
Thirty male albino wistarrats were divided randomly into six groups. Group I were treated with normal
saline, Group II were treated with Gentamicin for a period of 7 days, Group III treated with Cystone
(p.o), Group IV,V,VI was treated with plant extract 150, 300,600 mg/kg respectively for 14 days with
gentamicin treatment from day 7 to 14. All the animals were fed with caliculi Producing diet for entire
study period. Later, urine samples were collected at the end of the studyand analysed for renal function
parameters like Creatiinine, BUN, Serum Urea etc. The kidneys of the sacrificed animals were isolated
and sectioned for histopathological studies.
Results:
The rats treated with ethanolic extract of canthiumdicoccum at doses 150,300,600 mg/kg significantly
showed improved effect and prevented urolithasis in rats which is evident in the reduction of creatinine,
Urea and BUN levels when compared to gentamicin treated group. The histopathological studies also
showed less degenerative changes.It also decreased crystal deposition markedly into the renal tubules
in number as well as size and prevented damage to the renal tubules.
Conclusion:
The results demonstrated that the ethanolic extract had significantly reduced the urolithiasis that was
caused by an CPD diet combined with gentamicine injection in rats.
Keywords: Gentamicin, Anti-Urolithiatic activity, Canthiumdicoccum, ethanol.
EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF SALIX SUBSERRATA IN PARACETAMOL IN...gynomark
The present study was designed to evaluate the possible protective effect of ethanolic extract of Salix subserratainn (EESS) against paracetamol induced hepatoxicity in animals. A literature survey revealed that more studies were needed for this plant to ascertain the hepatoprotective potential. The detailed preliminary phytochemical investigations rationalized its use as a drug of therapeutic importance. Theethanolic extract of the planthas phytoconstituents like flavonoids, terpenoids, sterods, alkaloids, saponins and tannins. The hepatoprotective effect was assessed using a battery of biochemical and histopathological tests. SGOT, SGPT, ALP, LDH, ACP were some of the biochemical tests done. In vivo tests for antioxidants (SOD, CAT, GSH, LPO) were conducted on albino mice and wistar rats. In both paracetamol induced hepatotoxicity, a lower dose and a high dose of extract were used and compared with the hepatoprotective activity of standard drug silymarin. Control group and an only drug group were also used. EESS ethanolic extract has hepato protective effects against liver toxicity induced by TAA as proven bymacroscopical, microscopical, and biochemical analyses. The effects of EESS are comparable to that of Silymarin, the standard hepatoprotective drug. Accordingly, EESS could beused as an effective herbal product for the prevention of chemical-induced hepatic damage. In conclusion, we can say that has the ability to protect the liver from the damaging effects of paracetamol toxic doses and stimulation of endogenous anti-oxidant defense system.
Key words: Hepatoprotective Activity, Salix Subserrata, Paracetamol Induced, Wistar Rat
Evaluation of Hepatoprotective Activity and Oxidative Stress parameters of Al...gynomark
In spite of the tremendous advances made, no significant and safe
hepatoprotective agents are available in modern therapeutics.
Therefore, due importance has been given globally to develop plantbased
hepatoprotective drugs, effective against a variety of liver
disorders. The aim of the current study is to screen the alcoholic
extract of Artabotrys hexapetalus (L.f) Bhandari (AEAH) for
hepatoprotective activity in rats which were intoxicated by
paracetamol. This article describes phytochemical (qualitative),
hepatoprotective activity and oxidative stress parameters of the above
selected plant drugs by studying the serum enzyme levels like SGOT,
SGPT, ALP, ACP, Total Bilirubin, Direct Bilirubin, SOD, GSH,
Vitamin C and Catalase levels of the animals treated with hepato
toxicant paracetamol. The alcoholic extract of AEAH reversed the
hepatotoxicity induced by paracetamol in the rats, indicating their
hepato-protective action. The study was also supported by the
histopathological studies which reversed structural damage occurred
due to paracetamol. This study was further supported by the DNA
fragmentation studies which showed the absense of fragmentation of
DNA in AEAH treated groups, indicating the hepatoprotective activity
of Artabotrys hexapetalus (L.f) Bhandari. Hence it can be concluded
that the plant extract possesses a promising hepatoprotective and
antioxidant effect.
Pharmcological screening of antidepressant activity of plant Tricholepis glab...gynomark
ABSTRACT
Tricholepis glaberrima (Asteraceae), popularly known as “Brahmdandi” has been used for the treatment of variety
of disease. The main objective of this research work was to evaluate the antidepressant activity of Tricholepis
glaberrima in rats. The study was undertaken to evaluate the possible antidepressant effect of Tricholepis
glaberrima aerial parts using forced swimming test and tail suspension test models of depression. Imipramine has
been taken as a standard drug with a dose of 10mg/kg, Group-1(untreated), group-2 (standard) received
imipramine orally. Group 3, 4 and 5 received METG at the doses of 200, 400 and 600mg/kg respectively.
Methanolic extract of aerial parts of Tricholepis glaberrima produced significant antidepressant like effect at the
dose of 600mg/kg in both models of FST and TST which indicated reduction in immobility time. The efficacy of
METG at 600mg/kg found to be comparable to that of standard drug Imipramine at 10mg/kg. The results of
present study indicated that methanolic extract of aerial parts of Tricholepis glaberrima possesses significant
antidepressant activity compared to that of standard drug imipramine.
KEYWORDS: Tricholepis glaberrima, Forced swimming test, Tail suspension test, Methanolic extract of
Tricholepis glaberrima, Imipramine.
The mood changes are part of our daily life, when
reactions to these situations become extreme that leads to
clinical condition called depression and it is associated
with lots of morbidity. Hence, it is very important to
address these problems and find effective remedies. Thus
the antidepressant study of tricholepis glaberrima was
done on different groups of white albino rats at the doses
of (200mg/kg, 400mg/kg and 600mg/kg) by using forced
swimming test (fst) and tail suspension test (tst). Results
showed that the administration of the methanolic extract
of tricholepis glaberrima (metg) produced a decreased
immobility time of rats and at the dose of 600mg/kg
produced significant antidepressant like effect in both
FST and TST models of depression and their efficacies
were found to be comparable to Imipramine (10mg/kg).
The results concluded that the shortening of immobility
time in the (FST) and (TST) mainly depends on the
enhancement of central 5HT and catecholamine
neurotransmitters, these effects are thought to be due to
the presence of chemical constituents like, alkaloids,
flavonoids and glycosides. Hence Tricholepis glaberrima
aerial parts extract possesses antidepressant effect in
animal models of depression. Further investigations in
this line is essential to establish its other therapeutic
benefits.
Phytochemical screening antiepileptic and analgesic activity of leaf extract ...gynomark
Antiepileptic activity of passiflora foetida was evaluated in mice against maximum
electroshock induced convulsions and pentylenetetrazole induced covulsions.Screening
of analgesic activity was evaluated using Eddy’s hot plate method using aspirin as
standard.Antispamodic activity was evaluated in mice using Diazepam as standard.
Hypnotic activity was evaluated using thiopentone as standard
evaluation of hepatoprotective activity of bauhinia purpurea linn.pdfgynomark
Present study was carried out to investigate different extracts of Bauhinia purpurea (B.P) for its
hepatoprotective activity against CCl4 induced hepatotoxicity. Mature leaves of Bauhinia purpurea were
collected, authenticated and was subjected to extraction using different solvents like chloroform, alcohol and
water. Healthy wistar albino rats (150-200g) of male sex were used for the in-vivo investigations. Liver damage
was induced by administration of 30% CCl4 suspended in olive oil (1ml/kg body weight). Activities of liver
marker enzymes, glutamate oxaloacetate transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT),
acid phosphatase (AP), alkaline phosphatase (ALP),total albumin(TA), total bilirubin(TB), Total protein(TP),
direct bilirubin (DB) at a dose of aqueous extract of leaves (100 mg/kg) chloroform extract of leaf of B.P
(100mg/kg and 150 mg/kg) and ethanol extract of leaf of B.P (100mg/kg and 150 mg/kg) showed a significant
hepatoprotective effect in comparison with the standard (sylimarin). It is also confirmed by liver
histopathology of treated animals. The present study demonstrated the extracts of B.P have hepatoprotective
effect against CCl4 induced hepatotoxicity.
The present study revealed a significant decrease in
the serum enzyme levels which can be attributed to
hepatoprotection. BP extract was found to decrease
the levels of ALP, ACP significantly and there is a
dose dependent decrease in the elevated SGOT and
SGPT levels of the extracts when compared to CCl4
group.
CCl4 treated Liver showed perivenular necrosis,
steatosis with degree of steatosis being variable
from ballooning degeneration to necrosis. Central
lobular vacuoles, frequently dilated and congested
central veins were seen with dilatation of
surrounding sinusoids, which contradicted to the
observations of standard sylmarin, the aqueous,
chloroform and alcoholic extracts showed a clear
portal tract and central vein with normal lobular
architecture and decreased cell degeneration
indicating the hepatoprotective action of extracts of
B.purpurea. The histopathological studies further
confirmed the above results presented in fig 1-8.
Therefore, from the above study the extracts of
Bauhinia purpurea exhibited potent
hepatoprotective activity against CCl4 induced liver
toxicity which can be ascribed to its ability to
decrease the oxidative damage.
Obesity is very serious and concerned problem these days. Despite availability of many drugs in market to treat
obesity, no single drug is ideal for treating all sorts of problems caused by obesity. The obesity models available
for inducing obesity are by using chemicals and high fat diet. Wistar albino rats were used to study anti-obesity
activity of methanolic extract of Tricholepisglaberrima plant aerial parts at doses 100 mg/kg p.o. and 200
mg/kg p.o. against the standard orlistat 50 mg/kg p.o. in models of anti-obesity activity viz. High fat induced
obesity, Monosodium glutamate induced obesity model. The induction of obesity is done by diet (20
grams/animal/day) and Monosodium glutamate (oral). The study period is 28 days for both models. In both
models, the plant showed anti-obesity activity significantly at a dose of 100mg/kg and 200 mg/kgp.o. by
reducing the body weight, fat pads weight, total cholesterol, triglycerides, LDL, VLDL, biomarkers enzymes like
SGOT, SGPT and ALP, whereas significant increase in HDL levels was observed. Further multiple dose preclinical studies and clinical studies have to be carried out for proving for human obesity treatment.
The biological activities of methanolic extract of
Tricholepisglaberrima observed in this study
strongly indicated their great potential as anti-obese
and obesity associated complications like
hyprlipidemia. Oral administration of extracts
reduced the level of circulating lipids significantly,
resulting in the decrease of body weights in various
animal models of obesity bearing close
resemblance to human obesity. Extract appear to
show such activities by modulating the lipid
metabolism through the decreased activity in
lipogenesis or by inhibition of pancreatic lipase
activity.
The methanolic extract of aerial parts of
Tricholepisglaberrima at a dose of 200mg/kg b.w.
p.o. significantly reduced total cholesterol,
triglycerides, LDL, VLDL, biomarkers enzymes
like SGOT, SGPT and ALP, whereas significant
increase in HDL levels was observed.
Phytoconstituents like saponins, tannins and
flavonoids in METG may be responsible for its anti-obesity and anti-hyperlipidemic activities by
multiple actions.
Apart from anti-obesity and anti-hyperlipidemic
agent, It may also act as hepatoprotective agent due
to possessing significant reduction in SGOT, SGPT
and ALP levels and significant increase in HDL
levels respectively.
Thus it can be said that METG is effective in
ameliorating abnormalities in lipid profile and fat
accumulation in rats and results provides useful
information for the clinical research that this plant
can be used as herbal drug in the treatment of
obesity and hyperlipidemia. Further studies on this
extract may be focused on the possible mechanism
of action, isolation, characterization and
purification of active constituents which is
responsible for anti-obesity and anti-hyperlipidemic
activities.
The present study reveals the acute toxicity of Diospyros malabarica fruit extract. No morbidity and mortality were observed at a higher dose of 2000 mg/kg throughout the 14 days observation period. This acute study helps to predict that it does not contain any type of toxicity and it it full safe. Gentamicin induced nephroxicity is characterized by elevated levels of urea, creatinine, uric acid, total protein sodium and potassium in serum as well as urine urea and creatinine, severe proximal tubularnecrosis, renal failure were found to be significantly increased in rats treated with only gentamicin. Similar pattern of changes were also observed in this study following gentamicin treatment. DM supplemation to GM treated rats recorded decrement in levels of urea, creatinine, uric acid, total protein, sodium and potassium in serum and also in urine urea and creatinine. These observations indicate an improved in renal function. GM administration to control rats produced a typical pattern of nephrotoxicity which was manifestated by marked increase in serum BUN. DM supplementations to GM treated rats recorded decrement in levels of blood urea nitrogen in plasma. Histopathological results demonstrating structural changes n renal tissue of aminoglycoside antibiotics such as GM were reported by some researches. Histopathological view of renal sections in GM treated groups showed the degeneration, desquamation and necrosis in tubules, blood vessel congestion and swelling in glomerulus, as compared to control groups. Groups treated with GM + DM 200 mg/ kg showed tubular necrosis, necrotic changes, karyopicnosis, glomeruli showed mesangeal matrix expansion. Glomerular and tubular epithelial changes were considerably mild in groups treated with GM + 400 mg/kg and GM + 600 mg/kg i.e animal treated with DM 400 mg/kg showed mild glomerular mesangeal matrix expansion, mild tubular epithelial changes and no congestion in blood vessels while in case of animal treated with DM 600 mg/kg showed regeneration in tubular epithelial cells. Thus, morphological changes in kidneys were because of GM administration, but these changes tended to be mild in GM + DM treatment.
EVALUATION OF ANTI-ALZHEIMER AND ANTIPARKINSON ACTIVITY OFETHANOLIC EXTRACT.pdfgynomark
Whole plant extract of celosia cristata linn showed anti alzheimer's and anti parkinson's activity as evidenced by the statistical data provided by the study
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
1. ISSN 2348-1218 (print)
International Journal of Interdisciplinary Research and Innovations ISSN 2348-1226 (online)
Vol. 6, Issue 4, pp: (217-224), Month: October - December 2018, Available at: www.researchpublish.com
Page | 217
Research Publish Journals
Protective Effect of Musa paradisiaca Fruit
Extract On L-Arginine Induced Acute
Pancreatitis In Rats
Veena Gadicherla1
, Siva R Challa2
, Basaveswara Rao M V3
, Veena Rani I4
,
Geetha Parvathi A5
1*
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Krishna University, Machilipatnam,
Andhra Pradesh, India
2
Department of Pharmacology, KVSR Siddhartha Institute of Pharmaceutical Sciences, Vijayawada, Krishna Dist.,
Andhra Pradesh, India
3
Department of Chemistry, Faculty of Sciences, Krishna University, Machilipatnam, Andhra Pradesh, India
4,5
Department of Pharmacology, SSJ College of Pharmacy, Vattinagulapally, Hyderabad, Telangana State, India
*
Corresponding author: Email id: anugad@gmail.com
Abstract: Musa paradisiaca often refereed as Banana is an ancient herbaceous flowering plant and the most earlier
crop to be cultivated. It is one the important component of diet for all classes of people worldwide. All the parts of
the plant is believed to have beneficial effects in different ailments and an excellent nutritional element. The
present study was aimed to find the protective effect of Musa paradisiacal fruit extract on acute pancreatitis in rats.
Male Sprague dawley rats were randomly divided in to 4 groups. Control group with normal saline; disease
control group where acute pancreatitis was induced by using a single dose of L-arginine (2.5g/kg b.w); Musa
paradisiacal fruit extract were prophylactically administered before the induction of acute pancreatitis at a dose of
200 and 400 mg/kg b.w orally in normal saline for 7 days. At the end of the study, blood samples and isolated
pancreas were subjected to different pancreatic, antioxidant and inflammatory biomarker analysis. The remaining
tissue was subjected to histopathological studies and DNA fragmentation assay for assessing the damage and
protection of the tissues.
The results of the study revealed that prophylactic administration of the extracts reduced the amylase and lipase
levels when compared to disease control group as well as improved the overall antioxidant status in a dose
dependent manner. Further, the extracts also showed a protective effect against apoptosis.
In conclusion, the present study suggest that administration of Musa paradisiacal fruit extract exhibited a
protective effect on acute pancreatitis and further investigation is required for its molecular based mechanisms.
Keywords: Musa paradisiacal, acute pancreatitis, free radicals, apoptosis.
I. INTRODUCTION:
Acute pancreatitis is a inflammatory disorder of exocrine pancreas with high mortality. It may be acute or chronic forms
depending on the pathogenesis [1]. Acute pancreatitis can be self limiting form to necrotizing pancreatitis where the
severity of the disease correlates with the degree of necrosis [2]. The pathogenesis of the disease is complicated and the
most accepted theory is the auto digestion of the tissue due to the release of pancreatic digestive enzymes [3]. Another
widely reported theory is the involvement of oxidative and nitrosative stress [4]. These free radicals have an important
role in inflammation and systemic complications of pancreatitis. They damage the tissue cells by attacking the
polyunsaturated fatty acids of the lipid membrane, structural and enzymatic proteins and DNA [4]. The treatment of acute
2. ISSN 2348-1218 (print)
International Journal of Interdisciplinary Research and Innovations ISSN 2348-1226 (online)
Vol. 6, Issue 4, pp: (217-224), Month: October - December 2018, Available at: www.researchpublish.com
Page | 218
Research Publish Journals
pancreatitis is limited to supportive and symptomatic therapies. Several antioxidants have been examined for their
protective actions against acute pancreatitis [5-8]. Use of agents with antioxidant and anti-inflammatory features with high
nutritional supplement might exercise a beneficial effect on acute pancreatitis.
Banana is an early cultivated plant and a major component of economy for various countries [9]. Banana belongs to the
species Musa paradisiaca of the family Musaceae and offer many healthy benefits. They act as a rich source of Vitamins,
potassium, calcium, phosphorus, magnesium etc [10]. All the parts of a banana plant possess certain medicinal properties,
and thus have respective medicinal applications. The fruit of Musa paradisiaca is an exceptional fruit offering different
forms of nutrition. It is an excellent source of potassium and a single banana is said to provide 23% of potassium on daily
basis [10], [11]. The flowers are useful in bronchitis, dysentery, and ulcers, whereas cooked flowers are given to people
with diabetes. The fruit and leaves of banana are useful in treating burns and wounds.]. The fruit has a mild laxative
property where as the plant sap is an astringent which is used in cases of hysteria, epilepsy, leprosy, fevers, hemorrhages,
dysentery and diarrhoea. The unripe fruits have been evaluated for its antihyperglycemic, antioxidant, wound healing,
hypolipidemic, hair growth promoter, cardioprotective and against thyroid dysfunction [12], [13],[14],[15]. Banana is also
considered to be a rich source of antioxidants [9]. Bioactive compounds in banana include, phenolic compounds,
carotenoids, biogenic amines, phytosterols. Thus, the present study was designed to evaluate the beneficial effects of
M.paradiscia fruit on L arginine induced pancreatitis in rats.
II. MATERIALS AND METHODS
A. Chemicals:
L-arginine was purchased from Sigma chemicals pvt ltd. Amylase estimation kit was purchased from Akray healthcare
pvt ltd. Lipase estimation kit was purchased from Aggape diagnostics ltd. C Reative protein (CRP) estimation kit from
Akray healthcare pvt ltd. DNA isolation kit was obtained from Bioartis pvt ltd. All other chemicals used in the study are
of analytical grade and were purchased from SD fine pvt ltd.
B. Animals:
Male Sprague dawley rats were selected for the study. They were housed in clean polypropylene cages at 25 ± 5o
C with
12 hr light and dark cycle and acclimatized for 1 week before the start of the experiment. The animals were fed on
standard pellet diet with water ad libitum. The study has been approved by Institutional animal ethics committee
(1448/PO/Re/S/11/CPCSEA/07/2016).
C. Plant material:
The unripe fruits of M.paradisica were collected from the local market. The fruits were shade dried, powdered and stored
till use. The dried powdered were subjected to soxhlation with ethanol for 72hrs. The resultant extract was evaporated to
dryness and stored at 4o
C till use. The fruit material was authenticated by taxonomist, Department of Botany, S V
University, Tirupati with voucher no 1003. The dried extract was re-suspended in normal saline and subjected for acute
toxicity studies by fixed dose method of OECD guideline 420. The LD 50 was found to be more than 2000 mg/kg and
1/10th
and 1/5th
dose of LD50 was used in the study.
D. Study protocol:
Animals were randomly divided into groups consisting of 6 animals each. Group I considered as control group received
normal saline orally throughout the study. Group II, disease control group received a single dose of 20% of L-arginine Hcl
[2x2.5 g/kg, intraperitonially (i.p), 1 hour apart] in 0.5% normal saline on day 5 of the study. Group III served as positive
control and administered Melatonin (10 mg/kg); Groups IV and V treatment groups received 200 and 400 mg/kg of
M.paradisica fruit extract (MPFE) orally for 7 days. On day 5 the positive control and treatment groups received a single
dose of 20% of L-arginine Hcl in 0.5% normal saline 1 hr after the administration of the extracts. At the end of the study
on day 7, blood samples were collected from retro-orbital plexus for the enzyme estimations. The animals were sacrificed,
pancreas were isolated and subjected for tissue enzyme estimation, histopathological study and DNA fragmentation assay.
E. Biochemical enzyme estimations:
Serum amylase, C-reactive protein (CRP) and lipase were estimated by the use of commercial kits from Akray healthcare
pvt ltd and Aggape diagnostics ltd. respectively.
3. ISSN 2348-1218 (print)
International Journal of Interdisciplinary Research and Innovations ISSN 2348-1226 (online)
Vol. 6, Issue 4, pp: (217-224), Month: October - December 2018, Available at: www.researchpublish.com
Page | 219
Research Publish Journals
Briefly, serum amylase was estimated by mixing 20µl of serum with 1000µl of amylase mono reagent and the absorbance
was read at 405nm after 60 seconds.
Serum lipase was measured by taking 20µl of the serum and to it 1000µl of reagent 1 was added and incubated it for 1-5
minutes at 37o
C. 250µl of reagent 2 was added and incubated it for another 2 minutes. The resultant solution was checked
for absorbance at 580nm.
CRP was estimated quantitatively by preparing a series of dilutions of the test serum in normal saline (eg: 1:2, 1:4, 1:8
etc) and to which one drop of CRP latex reagent was added. The formation of agglutination on the glass slide was taken as
the highest titre for CRP and represented as factor of 6 with units in micrograms /ml.
Antioxidant enzymes superoxide dismutase (SOD), was measured by reported method according to Misra and Fridovich,
1977 [16], Catalase estimated as per the method by Beers and Sizer, 1952 [17] and Vitamin C levels were estimated by
method reported by Omaye et al., 1979 [18].
Tissue enzyme estimations were estimated by homogenizing a part of the tissue and their supernatants were used for the
estimation of Glutathione, Lactate dehydrogenase (LDH), Myeloperoxidase (MPO), Malonylaldehyde (MDA) and nitrite
levels as per the reported methods [19], [20], [21], [22], [23].
F. DNA fragmentation Assay:
The DNA fragmentation assay was performed on SDS page gel electrophoresis. Briefly, the DNA from the pancreatic
tissue was isolated by using the kit by Bioartis pvt ltd. The isolated DNA pellet is air dried and resuspended in TE buffer
[pH-8.0] and 1mM EDTA. The resuspended DNA is loaded on to the SDS Page Gel electrophoresis for analysis [24].
G. Histopathological studies:
Paraffin sections of 5 µm were cut and stained with hematoxylin and eosin and then assessed under dark field microscope
for histopathological changes. Pancreatic damage was assessed for acinar cell degeneration, interstitial inflammation,
edema, and hemorrhage.
H. Statistical analysis:
All the values are expressed as Mean ± SEM. Statistical Analysis was performed by one way ANOVA followed by
Dunett’s Multiple Comparison Test using Graph pad Prism 5 software with statistically significance up to p < 0.001.
III. RESULTS
To evaluate the protective effect, rats were pretreated with MPFE as described in the experimental design. L arginine was
used for inducing acute pancreatitis and few hours after the induction, blood and tissue samples were collected and
subjected for enzymatic analysis.
Rats in the disease control group showed an increase in the levels of serum amylase and lipase significantly [p<0.001]
when compared to control and melatonin treated rats indicating the induction of pancreatitis [Table I]. The levels of SOD,
Catalase, Vitamin C and glutathione in disease control rats were significantly lower than the control group of rats [Table
II]. However, the levels of MDA, MPO, CRP, Nitrite levels were higher indicating the involvement of inflammation
[Table III].
In contrast with the disease control group, Melatonin and MPFE treated rats showed a significant protection against
damage to pancreas in dose dependent manner. The serum amylase and lipase levels were significantly [p<0.001] lower
than the disease group of rats but a little higher than the control group [Table I]. The levels of SOD, Catalase, Glutathione
and Vitamin C were higher in the MPFE treated rats indicating the antioxidant activity of MPFE and Melatonin [Table II].
The levels of MPO, MDA, CRP, Nitrite were significantly [p<0.001] reduced in MPFE treated rats dose dependently and
the same was observed in melatonin treated rats indicating their anti-inflammatory effects [Table III.]. Although the levels
of enzymes in MPFE treated rats differ significantly when compared to control group of rats.
In addition to this, Histopathological studies were carried out on the isolated pancreatic tissue by using hematoxylin and
eosin stain. Pancreas of rats treated with L-arginine showed necrosis of the tissue with vacuolar degeneration and
infiltration of leucocytes, whereas control, melatonin and MPFE treated rats showed a normal echotexture with retained
cell membrane and no vacuolar degeneration [Fig. 1]. Further, DNA Fragmentation assay was carried out for detection of
4. ISSN 2348-1218 (print)
International Journal of Interdisciplinary Research and Innovations ISSN 2348-1226 (online)
Vol. 6, Issue 4, pp: (217-224), Month: October - December 2018, Available at: www.researchpublish.com
Page | 220
Research Publish Journals
apoptosis. DNA of L-arginine group of rats showed extensive damage which is depicted as a smear pattern in the gel
picture. In disparity to it the control group, melatonin and MPFE treated rats had intact DNA when compared to disease
control [Fig.2]
IV. DISCUSSION
The present study established the protective effect of MPFE on L-arginine induced acute pancreatitis in rats. Acute
pancreatitis induced by L-arginine is a dose dependent induction model with hyperamylasemia and the histopathological
findings are similar to Human disease [25]. After the initial attack of pancreatitis, within 4-8 hrs the amylase and lipase
levels were increased swiftly reaching its peak at 24hours [25]. This observation is consistent with the present study
where L-arginine administration increased the levels of amylase and Lipase. Melatonin and MPFE administration reduced
the levels of amylase and lipase eliciting its protective effects. M.paradisica has been reported to inhibit serum α-amylase,
lipase in diabetic rats and has been suggested that the presence of of polyphenolic compounds could attribute to its
inhibitory effect [26], [27].
Free radical generation and depression of antioxidant defense have been reported to be central mediators of pancreatic
tissue damage in acute pancreatitis [28]. The generated oxygen and nitrogen free radicals at the early onset of the disease,
damage the mitochondria, activate nitric oxide synthase and polymorphonuclear leucocytes there by triggering various
inflammatory markers. Thus, oxidative and nitrosative stress, mark as a significant role in the pancreatic damage and extra
organ manifestations [29]. Further, the reactive oxygen species directly target the phospholipids and increase the levels of
MDA and MPO which are the byproducts of lipid peroxidation. In the current investigation, L-arginine increased the
levels of MDA, MPO, Nitrate, CRP and LDH levels and reduced the levels of SOD, Glutathione, Catalase and Vitamin C
enlightening its role in stimulating the oxidative stress. Melatonin, in contrast have reduced the oxidative stress levels and
improved the levels of antioxidant enzymes and is in accord with the previous reports [30]. MPFE administration
reversed the conditions by improving the levels of SOD, Catalase, Glutathione and Vitamin C which form a mutual
supportive team in eradicating the free radicals which was observed as reduced levels of MDA, MPO, Nitrate, CRP and
LDH levels. The results are in consistent with the previous reports on M.paradisica fruit for its antioxidant and anti-
inflammatory properties [31],[32]. The activities were suggested due to the presence of vitamin C, Kaempferol,
Myrecetin, Quercetin and rutin flavonoids as their major constituents [33].
In addition to these estimations, the study also explored the protective effects on apoptotic damage of pancreas by using
DNA fragmentation assay. The presumed consequence of apoptosis was damage to mitochondria due to increased calcium
uptake triggering the apoptotic processes [34], [35]. This is visualized as a fragmented DNA with a smear pattern on the
gel electrophoresis in rats treated with L-arginine. In contrast to this, pancreas of control and MPFE treated rats showed
an intact DNA eliciting its protective effects. Further, the histopathological findings also suggest a positive role of MPFE
in eradicating the inflammation and maintaining the normal echotexture and integrity of the pancreas when compared to
L-arginine treated group of rats.
V. CONCLUSION
The present investigation concludes that MPFE administration has protective effects against L-arginine induced
pancreatitis and it may be due to its antioxidative and anti-inflammatory effects. However, there is a need to explore in
depth molecular mechanism for its ability as a new supplementation in acute pancreatitis.
REFERENCES
[1] Sidhu, S., Pandhi, P., Malhotra, S., Vaiphei, K., & Khanduja, K. L. (2011). Beneficial effects of Emblica officinalis
in L-arginine-induced acute pancreatitis in rats. Journal of medicinal food, 14(1-2), 147-155.
[2] Lacy PE. “The pancreas. In: Anderson’s Pathology”, 9th ed. CV Mosby Co., St. Louis, 1990, pp. 871–919.
[3] Greenberger NJ, Toskes PP. “Acute and chronic pancreatitis. In:Harrison’s Principles of Internal Medicine (Hauser
S, Longo D,Jameson J, eds.)”. McGraw-Hill, New York, 2005, pp. 1895–1906.
[4] Szabolcs, A., Reiter, R. J., Letoha, T., Hegyi, P., Papai, G., Varga, I. & Lonovics, J. (2006). Effect of melatonin on
the severity of L-arginine-induced experimental acute pancreatitis in rats. World journal of gastroenterology: WJG,
12(2), 251.
[5] Czako L, Takacs T, Varga IS, Tiszlavicz L, Hai DQ, Hegyi P, Matkovics B, Lonovics J. (2000). Oxidative stress in
distant organs and the effects of allopurinol during experimental acute pancreatitis. Int J Pancreatol; 27: 209-216
5. ISSN 2348-1218 (print)
International Journal of Interdisciplinary Research and Innovations ISSN 2348-1226 (online)
Vol. 6, Issue 4, pp: (217-224), Month: October - December 2018, Available at: www.researchpublish.com
Page | 221
Research Publish Journals
[6] Virlos I, Mazzon E, Serraino I, Di Paola R, Genovese T, Britti D, Thiemerman C, Siriwardena A, Cuzzocrea S.
(2003). Pyrrolidine dithiocarbamate reduces the severity of cerulein-induced murine acute pancreatitis. Shock 20:
544-550
[7] Wenger FA, Kilian M, Jacobi CA, Gregor JI, Guski H, Schimke I, Muller JM. (2002). Effects of octreotide on lipid
peroxidation in pan creas and plasma in acute hemorrhagic necrotizing pancreatitis in rats. Pancreatology 2: 211-
216
[8] Yagci G, Gul H, Simsek A, Buyukdogan V, Onguru O, Zeybek N, Aydin A, Balkan M, Yildiz O, Sen D. (2004).
Beneficial effects of N-acetylcysteine on sodium taurocholate-induced pancreatitis in rats. J Gastroenterol 39: 268-
276
[9] Thompson, A.K. 2011. “Postharvest Biology and Technology of Tropical and Subtropical Fruits Banana (Musa
Spp.)” .Woodhead Publishing Limited..pg. ??
[10] Kumar, K. S., Bhowmik, D., Duraivel, S., & Umadevi, M. (2012). Traditional and medicinal uses of banana. Journal
of Pharmacognosy and Phytochemistry, 1(3), 51-63.
[11] Rajesh, N. (2017). Medicinal benefits of Musa paradisiaca (Banana). International Journal of Biology Research.
Volume 2; Issue 2;; Page No. 51-54.
[12] Yakubu, M. T., Nurudeen, Q. O., Salimon, S. S., Yakubu, M. O., Jimoh, R. O., Nafiu, M. O., ... & Williams, F. E.
(2015). Antidiarrhoeal activity of Musa paradisiaca Sap in Wistar rats. Evidence-Based Complementary and
Alternative Medicine, 2015.
[13] Kumar, S., Mishra, C., Ahuja, A., Rani, A., & Nema, R. K. (2012). Phytoconstituents and Pharmacological activities
of Musa paradisiaca Linn. Asian Journal of Biochemical and Pharmaceutical Research, 4(2), 199-206.
[14] Vijayakumar, S., Presannakumar, G., & Vijayalakshmi, N. R. (2008). Antioxidant activity of banana flavonoids.
Fitoterapia, 79(4), 279-282.
[15] Parmar, H. S., & Kar, A. (2007). Protective role of Citrus sinensis, Musa paradisiaca, and Punica granatum peels
against diet-induced atherosclerosis and thyroid dysfunctions in rats. Nutrition Research, 27(11), 710-718.
[16] Misra HP, Fridovich I. (1977). Superoxide dismutase: a photochemical augmentation assay. Archives of
Biochemistry and Biophysics. May 1;181(1):308-12.
[17] Beers RF, Sizer IW. (1952). A spectrophotometric method for measuring the breakdown of hydrogen peroxide by
catalase. J Biol chem. Mar 1;195(1):133-40.
[18] Omaye ST, Turnbull JD, Sauberlich HE. (1979). Selected methods for the determination of ascorbic acid in animal
cells, tissues, and fluids. In Methods in enzymology Jan 1 (Vol. 62, pp. 3-11). Academic Press.
[19] Sedlak J, Lindsay RH. (1968). Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with
Ellman's reagent. Analytical biochemistry. Jan 1;25:192-205.
[20] Bergmeyer, H.U., and Bernt, E.(1974). “In Methods of Enzymatic Analysis”;2nd ed.; Academic Press: New York,
NY, Volume II, 574-579.
[21] Haqqani AS, Sandhu JK, Birnboim HC. (1999). A myeloperoxidase-specific assay based upon bromide-dependent
chemiluminescence of luminol. Analytical biochemistry. Aug 15;273(1):126-32
[22] Ohkawa H, Ohishi N, Yagi K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction.
Analytical biochemistry. Jun 1;95(2):351-8.
[23] Laura C.Green David A.Wagner Joseph Glogowski Paul L.Skipper John S.Wishnok Steven R.Tannenbaum. (1982).
Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Anal Biochem. Oct;126(1):131-8
[24] Alexei G. Basnakian and S.Jill James. (1994). A rapid and sensitive assay for the detection of DNA fragmentation
during early phases of apoptosis. Nucleic Acids Research, Vol. 22, No. 13 pg no. 2714-15
[25] Hegyi, P., Rakonczay Jr, Z., Sári, R., Góg, C., Lonovics, J., Takács, T., & Czakó, L. (2004). L-arginine-induced
experimental pancreatitis. World journal of gastroenterology: WJG, 10(14), 2003.
6. ISSN 2348-1218 (print)
International Journal of Interdisciplinary Research and Innovations ISSN 2348-1226 (online)
Vol. 6, Issue 4, pp: (217-224), Month: October - December 2018, Available at: www.researchpublish.com
Page | 222
Research Publish Journals
[26] Shodehinde, S. A., Ademiluyi, A. O., Oboh, G., & Akindahunsi, A. A. (2015). Contribution of Musa Paradisiaca in
the inhibition of α-amylase, α-glucosidase and angiotensin-I converting enzyme in streptozotocin induced rats. Life
sciences, 133, 8-14.
[27] Eleazu, C., & Okafor, P. (2015). Use of unripe plantain (Musa paradisiaca) in the management of diabetes and
hepatic dysfunction in streptozotocin induced diabetes in rats. Interventional Medicine and Applied Science, 7(1), 9-
16.
[28] Rattan, S. I. (2006). Theories of biological aging: genes, proteins, and free radicals. Free radical research, 40(12),
1230-1238.
[29] Abdin, A. A., El-Hamid, M. A. A., El-Seoud, S. H. A., & Balaha, M. F. (2010). Effect of pentoxifylline and/or alpha
lipoic acid on experimentally induced acute pancreatitis. European journal of pharmacology, 643(2-3), 289-296.
[30] Sidhu, S., Pandhi, P., Malhotra, S., Vaiphei, K., & Khanduja, K. L. (2010). Melatonin treatment is beneficial in
pancreatic repair process after experimental acute pancreatitis. European journal of pharmacology, 628(1-3), 282-
289.
[31] Dikshit, P., Tyagi, M. K., Shukla, K., Gambhir, J. K., & Shukla, R. (2016). Antihypercholesterolemic and
antioxidant effect of sterol rich methanol extract of stem of Musa sapientum (banana) in cholesterol fed wistar rats.
Journal of food science and technology, 53(3), 1690-1697.
[32] Rao, U. M., Ahmad, B. A., & Mohd, K. S. (2016). In vitro nitric oxide scavenging and anti-inflammatory activities
of different solvent extracts of various parts of Musa paradisiaca. Malaysian J. Anal. Sci, 20(5), 1191-1202.
[33] Shodehinde, S. A., & Oboh, G. (2013). Antioxidant properties of aqueous extracts of unripe Musa paradisiaca on
sodium nitroprusside induced lipid peroxidation in rat pancreas in vitro. Asian pacific journal of tropical
biomedicine, 3(6), 449.
[34] Wen L, Mukherjee R, Huang W, Sutton R. (2016). Calcium signaling, mitochondria and acute pancreatitis: avenues
for therapy. Pancreapedia: The Exocrine Pancreas Knowledge Base. Jul 30.
[35] Kang, R., Lotze, M. T., Zeh, H. J., Billiar, T. R., & Tang, D. (2014). Cell death and DAMPs in acute pancreatitis.
Molecular medicine, 20(1), 466.
TABLE I: CHANGES IN THE LEVELS OF AMYLASE AND LIPASE IN RATS TREATED WITH L-ARGININE AND
DIFFERENT DOSES OF THE MPFE EXTRACTS.
Serum Enzyme
Normal
Control
Disease
Control
Melatonin
10 mg/kg
MPFE
200 mg/kg
MPFE
400 mg/kg
Amylase (IU/L) 14.17 ± 0.116#
330.5 ± 3.233*
98.8 ± 2.234*#
202.5 ± 6.091*
161.5 ± 7.714*
Lipase (U/L) 16.17 ± 0.9804#
83.17 ± 1.138*
34.45 ± 1.456*#
58.16 ± 2.32*#
42.5±3.082*#
Values are expressed in Mean ± SD (n=6); *, #
p <0.001 when compared to Normal Control and Disease control
group respectively.
TABLE II: CHANGES IN THE LEVELS OF ANTIOXIDANT ENZYMES IN RATS TREATED WITH L-ARGININE AND
DIFFERENT DOSES OF THE MPFE EXTRACTS.
Antioxidant
enzyme
Normal
Control
Disease
Control
Melatonin
10 mg/kg
MPFE
200 mg/kg
MPFE
400 mg/kg
SOD
(mg/protein/min)
18.5 ± 0.4282#
6.167 ± .3073*
61.23 ± 1.724#*
25.6±2.66*#
40.3±2.50*#
Catalase
(µM of
H2O2/mg/protein
/minute)
48.18± 0.3056#
22.7± 0.2811*
47.14 ± 0.234#
36.16±1.17*
41.83±2.04#
Glutathione
(mg/dl)
2236± 0.9574#
575.8± 1.493*
3124 ± 3.549#*
2170.8±4.66#
2292.5±2.88#
Vitamin C
(mg/dl)
95.73 ± 0.3509#
21.35 ± .5771*
212.34±0.6784*#
181.66 ±0.876*#
228.8 ±1.812*#
Values are expressed in Mean ± SD (n=6); *, #
p<0.001 when compared to Normal Control and Disease control
group respectively.
7. ISSN 2348-1218 (print)
International Journal of Interdisciplinary Research and Innovations ISSN 2348-1226 (online)
Vol. 6, Issue 4, pp: (217-224), Month: October - December 2018, Available at: www.researchpublish.com
Page | 223
Research Publish Journals
TABLE III: CHANGES IN THE LEVELS OF TISSUE BIOMARKERS IN RATS TREATED WITH L-ARGININE AND
DIFFERENT DOSES OF THE MPFE EXTRACTS.
Tissue marker
Normal
Control
Disease
Control
Melatonin
10 mg/kg
MPFE
200 mg/kg
MPFE
400 mg/kg
MDA
(mM/dl/hr)
14.89± 0.3381#
86.87± 0.8747*
35.56 ± 0.9852*#
21.08±1.05#
14.53±0.99#
Nitrate
(µM/g)
11.08 ± 0.212#
35.05 ± .1522*
14.34 ± 0.8765#
26.85±0.40*#
18.48±0.77#
CRP
(µg/ml)
486.8± 2.358#
19601± 2.455*
697 ± 4.345*#
1596.6 ±4.92*#
1301±3.34*#
MPO
(µM of peroxide/min)
4.032± 0.05486#
25.69± 0.3327*
6.45 ± 0.4563#
11.97 ± 0.2828#
7.902 ±0.2765#
LDH
(U/L)
23.4± 0.5379#
127.5± 0.3785*
45.56 ± 0.8976*#
95.75±1.127*#
71.86±2.55*#
Values are expressed in Mean ± SD (n=6); *, #
p<0.001 when compared to Normal Control and Disease control
group respectively.
Fig 1: Histopathologgical changes in Pancreas of rats treated with L-arginine and MPFE extracts
Fig. 1. Histopathological findings of formalin fixed paraffin embedded section of pancreas with Hematoxylin and
Eosin. (A) Photomicrograph of the normal Pancreas (B) L-arginine induced pancreas showing extensive acinar
cell damage with interstitial edema, vacuolar degeneration. (C & D) protective effect of MPFE. Sections show
normal acinar cells as that of Normal pancreas.
8. ISSN 2348-1218 (print)
International Journal of Interdisciplinary Research and Innovations ISSN 2348-1226 (online)
Vol. 6, Issue 4, pp: (217-224), Month: October - December 2018, Available at: www.researchpublish.com
Page | 224
Research Publish Journals
Fig 2: Gel picture of DNA fragmentation Assay
Figure 2: Gel picture of DNA fragmentation assay of the pancreatic tissue.
1 – Group I; Control group pancreas showing intact DNA
2 – Group II; Disease control pancreas treated with L-arginine showing fragmented DNA in the form of smear
indicating extensive damage;
3, 4- Group IV & V; MPFE treated pancreas showing intact DNA eliciting its protective effect
5 – Group III; Melatonin treated pancreas showing intact DNA