This document discusses several physical differences between male and female spiders that can be used to determine their sex. It notes that females are generally larger than males, with some females being multiple times the size. It also describes differences in coloration, pedipalp size, venom sac size, abdomen size, use of pheromones, and various anatomical structures like the epigynum and epiandrous fusillae that vary between sexes. The document provides examples from species like black widows and golden silk spiders to illustrate these distinguishing physical traits.