Presentation UAPID 16-0049
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This document describes an educational research kit that uses CRISPR-Cas10 gene editing technology. The kit allows students to explore gene transfer between phages, which is currently not well understood. It leverages the accessibility of CRISPR to enable high school, college, and graduate students to learn about gene editing and DNA analysis of bacteria. The technology was successfully piloted and demonstrates that CRISPR research can be made accessible through an educational kit. The kit was invented by Dr. Asma Hatoum, an assistant professor of biological sciences.
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This resume is for Kalyan Chakravarthy, who is seeking a full-time job in the biomedical/pharmaceutical industry. He has a M.S. in Biomedical Engineering from Drexel University with a 3.8 GPA and a B.S. in Biotechnology from ICFAI Institute of Science and Technology with a 3.6 GPA. His experience includes research assistant roles at Drexel University, Merck Research Laboratories, Global Hospitals and Research Laboratories, and Center for Cellular and Molecular Biology. His skills include laboratory techniques, data analysis tools, and computer programs. He has authored publications and conference presentations related to plasma medicine, gene signatures, and compound screening.
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The document provides an overview of the CRISPR/Cas9 gene editing technology. It discusses the history and components of the CRISPR system, how it works, applications in various fields like microbiology, biomedicine, agriculture, and therapeutics. Recent advances expand its use for transcriptional regulation, epigenetic editing, and live imaging. While powerful, it faces challenges like off-target effects that require further research to optimize its safe and ethical application.
Considerations and challenges in building an end to-end microbiome workflow
Many of the data management and analysis challenges in microbiome research are shared with genomics and other life-science big-data disciplines. However there are aspects that are specific: some are intrinsic to microbiome data, some are related to the maturity of the field, with others related to extracting business value from the data.
CINECA webinar slides: Making cohort data FAIR
Cohort studies, which recruit groups of individuals who share common characteristics and follow them over a period of time, are a robust and essential method in biomedical research for understanding the links between risk factors and diseases. Through questionnaires, medical assessments, and other interactions, voluminous and complex data are collected about the study participants. While cohort studies present a treasure trove of data, the data is often not FAIR (findable, accessible, interoperable and reusable). First, due to the sensitive and private nature of medical information, cohort data are often access controlled. Due to the lack of information about the studies (metadata), often one needs to dig deep to know what data is available in a cohort study. Therefore, many cohort datasets suffer from the findable and accessible issues. Second, often data collection is performed with instruments and data specifications tailored to the study. As a result, combining data across cohorts, even ones with similar characteristics, is difficult, making interoperability and reusability a challenge. In this presentation, we will explore several informatics techniques, such as the use of ontology, to make cohort data more FAIR. We will also consider the implications of making cohort data more open and the ethical and governance issues associated with open science benefit sharing.
This webinar is part of the “How FAIR are you” webinar series and hackathon, which aim at increasing and facilitating the uptake of FAIR approaches into software, training materials and cohort data, to facilitate responsible and ethical data and resource sharing and implementation of federated applications for data analysis.
The CINECA webinar series aims to discuss ways to address common challenges and share best practices in the field of cohort data analysis, as well as distribute CINECA project results. All CINECA webinars include an audience Q&A session during which attendees can ask questions and make suggestions. Please note that all webinars are recorded and available for posterior viewing. CINECA webinars include an audience Q&A session during which attendees can ask questions and make suggestions.
This webinar took place on 17th February 2021 and is part of the CINECA webinar series.
For previous and upcoming CINECA webinars see:
https://www.cineca-project.eu/webinars
Grand round whsiao_may2015
Genomic epidemiology uses whole genome sequencing data from pathogens combined with epidemiological investigations to track the spread of infectious diseases. The document discusses making genomic epidemiology a widespread reality in public health. It outlines key requirements including building a user-friendly analysis platform, developing portable analysis pipelines, providing training to public health personnel, and improving information sharing between organizations.
How Can We Make Genomic Epidemiology a Widespread Reality? - William Hsiao
The document discusses genomic epidemiology and the requirements to bring genomic sequencing into routine public health practice. It outlines two parts: (1) what genomic epidemiology is and why it is important; and (2) the requirements for genomic sequencing to be used routinely in public health. Whole genome sequencing is seen as a way to generate high quality pathogen genomes quickly and allow for more detailed tracking of disease spread compared to traditional methods. However, bringing genomic sequencing into public health practice requires overcoming barriers such as the need for user-friendly analysis platforms, training public health personnel in genomics, and improving information sharing between organizations.
"CRISPR" Gene Editing Tool
The document summarizes a seminar presentation on CRISPR gene editing. It discusses how CRISPR/Cas systems in bacteria provide adaptive immunity by integrating fragments of viral DNA. It then describes some applications of CRISPR technology, including inactivating genes in human and other cells, modifying crops and mosquitoes. Recent developments include licensing of CRISPR kits and using CRISPR/Cas9 to introduce targeted mutations in model organisms and cell lines. Chinese researchers also used CRISPR/Cas9n to produce cattle with increased resistance to bovine tuberculosis by inserting a new gene.
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CRISPR Gene Editing Congress, 25-27 February 2015 in Boston, MA
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Presentation UAPID 16-0049
- 1. UAIPD 16-0049 CRISPR-Cas10 Gene Editing Educational Research Kit Dr. Asma Hatoum Triage Name February 17, 2017
- 2. Current Problems • Gene transfer between phages is a process that is not currently well understood • Gene editing market is expected to be $5.54 billion by 2021 • Leaders include Thermo Fisher Scientific, Merck KGAA, Lonza, & Agilent Technologies
- 3. • Two week phage recovery research kit which allows exploration of gene editing through CRISPR-10 CRISPR-CAS10 Gene Editing Educational Research Kit
- 4. • Our CRISPR-Cas10 technology leverages the ease, low-cost, and accessibility of CRISPR- based research on a large scale • High school, college, and graduate students can learn about CRISPR and simultaneously contribute to DNA analysis of Staphylococci. • The technology had success in a pilot program demonstrating the accessibility of the kit Advantages
- 5. • Inventor: Dr. Asma Hatoum • Assistant Professor • Biological Sciences • ahatoum@ua.edu Inventor
- 6. Office for Technology Transfer 2704 8th street Tuscaloosa, AL 35401 16-0049 OTT@UA.edu www.OTT.UA.edu
Editor's Notes
- Make sure to list references for all numbers, trends, etc. on each slide. Many inventors like to see exactly where the data comes from.
- Should read like Wikipedia entry. Try to focus on the high points.
- Make sure to list references for all numbers, trends, etc. on each slide. Many inventors like to see exactly where the data comes from.