enfleurage method, preparation of scented candle , evaluation parameters of volatile oils.

1. PREPARATION AND EVALUATION OF
POLYHERBAL SCENTED CANDLE
USING VOLATILE OILS EXTRACTED
BY ENFLEURAGE METHOD
SUBMITTED BY :
PUTTAMREDDY KAVYASRI​ (62001711)
BANAGANAPALLISHAIK SONY(62001725)
AAMUDALA SANDHYA(620001731)
B.PHARMACY(4TH YEAR).
SVU School of pharmaceutical sciences(SVU)
UNDER GUIDANCE OF
T.USHA KIRAN REDDY
M.PHARM(PHD)

2. 2
SVU PHARMACEUTICAL
SCIENCE,TIRUPATI
6/5/2023
CONTENTS:
1. INTRODUCTION
2. REVIEW OF LITERATURE
3. PLANT PROFILE
4. AIM AND OBJECTIVE
5. PLAN OF WORK
6. MATERIALS AND METHODS
7. CONCLUSION
8. REFERENCE.

3. INTRODUCTION:
MEDICINAL HERBS:
Medicinal plants are those plants rich in
secondary metabolites and potential sources of
drugs .
According to WHO a medicinal plant is any
plant which in one or more of its organs/parts
contain substances that can be used for therapeutic
purpose .
Eg: 1.Eucalyptuas leave used as traditional
medicine for cough and cold.
2.orange essential oil can be used for reducing
stress and it adds a fresh citrus aroma to room
3. Tulasi used for indigestion, heart health,
respiratory diseases

4. VOLATILE OIL/ ETHEREAL
OILS/ ESSENTIALOILS:
They evaporate when exposed to the at
ordinary temperature so they are called
volatile oils. They represents the essence of
plant so they called as essential oil.
SOURCES OF VOLATILE OIL
Dried ripe fruits : lemon oil.
Fresh leaves: eucalyptus oil
Ripe bulb: garlic oil
Methods of extraction of volatile oils
1. steam distillation
2. solvent extraction
3. enfleurage method
4. maceration method
USES OF VOLATILE OILS:
• The oil is responsible for different scents
that plant emits.
• They were widely used in cosmetics industry,
perfumes and also aroma therapy

5. Enfleurage is a process that
uses odourless fats that are solid
at room temperature to capture the
fragnant compounds excluded by
plants.
In this method essential oils are
extracted from fatty materials
using a solvent such as alcohol.
ENFLEURAGE METHOD:

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REVIEW OF LITERATURE
Amit Tiwari et al., (2016). Tagetes minuta L., commonly known as African marigold, is reputed as a source of ‘Tagetes oil’ of
trade that finds an extensive use in food, flavouring, pharmaceutical, perfumery and cosmetic industry. In present study,
variation in the essential oil content and composition of T. minuta grown in foot hills agroclimatic conditions of northernIndia and
harvested in flower initiation, full flowering, late flowering and seed setting stages were compared and analysed using GC-FID
and GC-MS.
Vipin Kesharwani et al., (2018). Eucalyptus globulus a widely observed plant has a tremendous latent in term of medicinal
uses. Eucalyptus globulus plant from family Myrtle, commonly known as blue gum grows well in Nilgiris, Annamalai, Palani
and Shimla hills. It rich sources of phytochemical constituents which contain flavonoids, alkaloids, tannin and propanoids.
Which are present in leaves, steam, and root of the plant. They are various properties like anti-inflammatory, antibacterial,
antiseptic, astringent, anti-diabetic, antioxidative, antiviral, antitumor, antihistaminic, anticancer cytochrome p450 inhibitor and
hepatoprotective effect have also been reported by many re searcher. The present review articlecritically discusses about various
phytochemical associated with the plant along with numeroustherapeutics application exhibited by the plant.
N Z Immaroh et al., (2021). Reviewed on Eucalyptus globulus (one of the species Eucalyptus)which is an essential oil-
producing plant that widely used in the medical sector. Generally, theuseful part of Eucalyptus is leaves. The presence of these
bioactive compounds made ofessential oils can be used as an antibacterial, antioxidant, and anti-inflammatory. The extraction
method has a major effect on the production of essential oil because it can affect yield and bioactive compound. The extraction
methods can be used to produce essential, such as maceration, Ultrasound-Assisted Extraction (UAE), Microwave Assisted
Extraction (MAE), water distillation, steam distillation, water-steam and distillation. This review discusses the extraction
methods and the main factor can affect the extraction process to produce E. globulusessential oils.

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Calendula officinalis
Kingdom : Plantae
Division : Magnoliophyta
Class : Magnoliopsida
Order : Asterales
Family : Asteraceae
Genus : Calendula L.
Species : Calendula officinalis
Common name
India :Marigold
French : TagetePatula
Hindi : Gainda
Africa :Tagetes Erecta
English :Marigold
Medicinal uses:
Jaundice,liver problem ,immune
boosting,eye infection,skin
treatment

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EUCALYPTUS GLOBULUS
Taxonomy:
kindom :plantae
Division : magnoliphyta
Class : dicotyledons
Sub class :
rosidaeorder:myrtales
Family: myrtaceae
Genious: eucalyptus
Species: eucaluptus
globuluslabill
Common names:
India: Tailapatra
Hindhi: Niligiri
English: Tasmanianbluegum
French: Arbre ac caoutchouc
Tamil :pothunalam
Medicinal uses:
Antimalarial, antihistaminic, antiseptic, anticancer,
antidiabetic, asthama.

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CITRUS SINENSIS
Taxonomy
Kindom: plantae
Division1: Magnoliophyte
class: magnoliophyte
Sub class: rosidae
Order: Sapindales
Family:rutaceae
Genus: citrus
Species: sinensis
Common names:
India : mosambi
French : les oranges
English : citrus sinensis
Hindhi : malta
Medicinal uses:
Asthma, Alzheimer’s , parkinsons disease , gallstones, optimal lung
function

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SVU COLLEGE OF PHARMACUTICAL SCIENCES,TIRUPATI.
6/5/2023
AIM AND OBJECTIVE
AIM:
The aim of the present study is to extract volatile oils from the herbs like eucalyptus ,orange,
marigold, which is further study for phytochemical analysis and FTIR studies, preparation of
scented herbal candle.
OBJECTIVES:
• Identification of plant materials and authentication of the scented plant
• Collection of fresh herbs
• Extraction of volatile oil using enfleurage method.
• Preparation of bed using vegetable fat.
• Phytochemical screening
• Volatile oils subjected for UV and FTIR studies
• Praparation of a polyherbal scented candle.

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SVU
PHARMACEUTICAL
SCIENCES,TIRUPATI
2023
Stress –Relief Therapy
Preparation of Scented Candles Using Extracts
Extracts are used for Phytochemical Analysis, Detection of Volatile Oils , U.V And FTIR Studies
Filtered and Extracted on a Hot Plate at 40-60 C
Bed is Removed and Digested With 95% Ethanol for 1month
Fixing of Fresh Plant Material on the Bed and Changed for Every 2 Days Till 2-3 Months
Preparation of Wax Bed using Vegetable Fat for Enflurage Method
Collection and Authentication of Fresh Plant Material
Selection of the Plant Material
Plan of action:

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SVU
PHARMACEUTICAL
SCIENCE,TIRUPATI.
2023
Materials & Methods
Collection of the Plant Material
1. Marigold flower petals:
Fresh and healthy flower petals i.e., Marigold (Tagetes erecta) is collected from flower market of Tirupati.
2. Orange peels:
Fresh and healthy orange (citrus sinensis) peels collected from market of Tirupati.
3. Eucalyptus leaves:
Fresh leaves of eucalyptus (Eucalyptus globulus) are collected.
Chemicals Required
• Distilled water, Ethanol, Vegetable fat, Dil. HCL, Picric acid, Mayer’s reagent, Dragendroff’s reagent, Wagner’s reagent, Hager’s reagent, Molish reagent, Fehling’s
reagent, Barfoed’s solution, Sodium hydroxide, Chloroform, Biuret reagent, Nitric acid, Lead acetate, Sodium picrate,Ferric chloride
Materials Required:
• Test tubes, Weighing balance, Hot air oven, Whattman filter paper, Aluminum foil, Trays, Glass rod.

13. 13
SVU
PHARMACEUTICAL
SCIENCE,TIRUPATI.
2023
METHADOLOGY:
1. Preparation of vegetable fat bed
2. Add orange peels , eucalyptus
leaves and mari gold petals to the fat
bed upto 2-3 months every day.
3. Keep fat in alcohol for 1
month

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4. Collecting of extract by filtration
method
5. Collection of oil by evaporation
method
6. Phtochemical screening for
volatile oils

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7. UV analysis
8. FTIR ANALYSIS 9. Formulation of polyherbal scented
candle

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10. Fragnence test for candles

17. Formalation of polyherbal scented candle
F1 F2 F3
Q1 Eucalyptus leaf oil 1ml 1ml 1ml
Q2 Orange peels oil 1.5ml 1.5ml 1ml
Q3 Marrigold oil 1.5ml 1.5ml 1.5ml
Q4 - - - -
17 6/5/2023

18. Nature based polyherbal scented candle was successfully
developed in this research work. Evaluation tests found that
the drug was very effective and safe to use. Statistically it
indicates that the commodity being offered is really
successufull.no complaints about allergic effects and this is
absolutely healthy product. While the formulation gives a
fresh fragrance of herbs it will relieve the stress even the
wordings was climate conscious ,comitative and wallet safe
conclusion
18 6/5/2023

19. 19 SVU COLLEGE OF PHARMACUTICAL
SCIENCES,TIRUPATI.
6/5/2023
Reference
1.Dixit Priyanka, Tripathi Shalini, Verma Kumar Navneet (2013). Botanical study of the marigold. International research journal of pharmacy
2.Tejal Rao (2010). The edible part of the calendula officinalis.
3.Barbara H. Smith, (2020) the height of the marigold.
4.Karthikeya Patil, CJ Sanjay, Nagabhushana doggalli, KR Renuka devi, N Harshitha. The chemical constituents present in the calendula officinalis. Review
article (2020).Nelofer Jan, Khurshid Iqbal Andrabi and Rifat john (2017). medicinal properties of the calendula officinalis. Review article from research gate
6.By AHPA (2015). Macroscopic and microscopic Characteristics of calendula officinalis.
7.Vipin kesharwani, Shashank Gupta, Nikhil Kushwaha, Roohi Kesarwani and Dilip KM Patel taxonomical classification, Chemical constituent of eucalyptus
globulus leaves and fruits and wood. International journal of herbal medicine (2018).
8.Vipin the chemical constitution and medicinal uses of eucalyptus globulus. Semantic scholar,
9.AHPA. (2015), macroscopic and microscopic characteristics of eucalyptus globulus.
10.Integrated taxonomical system- report taxonomical classification of citrus sinenesis
11.Juan Manuel J. favela-Hernandez, Omar Gonzalez-Santiago (2016) et., al. The chemical constituents present in citrus sinensis. Pub med central.
12.Mohamed hersi Farah (2020) the medicinal uses of the orange peel, the and macroscopic microscopic properties of the citrus sinensis.
13.Nayam Pushpa Sri A.S Dwi saptati N.H,dan Diah Agustina et.al.,(2016) the enfleurage method for extraction of volatile oil.
14.Sashikiran Palithya, Susmila Aparna Gaddam, Venkatasubbaiah kotakadi, Joshthna penchalaneni, Narasimha Golla, Suresh babu Naidu Krishna and C.V.
Naidu (2021) an international journal. The FTIR and U.V. Procedures.

20. Thank you
SVU COLLEGE OF PHARMACUTICAL SCIENCES,
TIRUPATI.