Anti-oxidant activity of ethanolic extract of roots of hiptage madablota gaertn

1. ANTI-OXIDANT ACTIVITY OF ETHANOLIC
EXTRACT OF ROOTS OF HIPTAGE
MADABLOTA GAERTN

2. A review on Hiptage madablota Gaertn (Madhavilata)
used as an ayurvedic drug
ABSTRACT: Hiptage madablota (L) Kurz belongs to the family Malphigiaceae. The plant has
strong therapeutic potential thus occasionally cultivated for medicinal purposes in several
traditional medicines to cure various diseases. This plant has been known to possess
antibacterial, antifungal, anti-diabetic, anti inflammatory, anti-cancerous, anti
mutagenic and hepatoprotective activity. According to Ayurveda, Hiptage madablota is cooling,
vulnerary, astringent, expectorant, cardiotonic, anti-inflammatory, insecticidal, wound healing
and used in burning sensation of the body, wound, pruritus, foul ulcers, scabies, leprosy, skin
diseases, cough, asthma, cardiac debility, rheumatism, hyperdipsia, obesity,
intrinsic hemorrhage etc. The presented review summarizes the information concerning the
botany, ethno pharmacology, phytochemistry and biological activity of this plant.

3. PLANT CLASSIFICATION:
Hiptage madablota Gaertn.
Kingdom : Plantae – Plants
Subkingdom : Tracheobionta – Vascular plants
Division : Magnoliophyta – Flowering plants
Class : Magnoliopsida – Dicotyledons
Order : Polygalales
Family : Malpighiaceae
Genus : Hiptage Gaertn
Species : madablota
Common Name : Helicopter flower
Vernacular Names : Hindi - Madhmalti, Madhavilata
Tamil - Vasantakaala malligai, Karipakkukodi
Telugu - Bandi Guriginja, Kuru Venda, Sura Gata
Synonyms : Hiptage benghalensis

4. Origin and cultivation:
Hiptage benghalensis (L) Kurz is native to subtropical
and tropical Asia (parts of southern China, Taiwan, India,
Nepal, Indonesia, Malaysia, Myanmar, Philippines, Sri
Lanka and Thailand). It is found throughout India,
predominantly in Western Ghats, Konkan,
Deccan, Kumaon, Assam and Andaman island, chiefly in
damp places up to an altitude of 1500 m (Data Base and
API, 2008). Hiptage benghalensis plant is large,
, climbing shrubs. It is cultivated in gardens also
for its attractive flowers.
Botanical description:
Leaves simple, opposite, blade usually elliptic with an attenuate
tip. Flowers, fragrant, borne in compact axillary racemes. Corolla
of five free, elliptic to round, reflexed petals, white with one petal
yellow in the center, margins fringed. Fruit a samara with three
spreading, papery oblanceolate to elliptic wing. White and
fragrant flowers are borne in erect, pubescent racemes. Flowers
have a yellow centre and orbicular to elliptic petals that is hairy
outside. Seeds are subglobose (Warrier et al., 1995).

5. Ethnomedicinal uses of Hiptage madablota
Hiptage madablota is cultivated for medicinal purposes and holds a reputed position in
Indian medicine. The leaves and bark are hot, acrid, bitter, insecticidal, vulnerary and useful
in treatment of cough, burning sensation, and inflammation; it has the ability to treat skin
diseases, particularly useful for dermatitis. An application made out of the plant is highly
beneficial in scabies. It's bark is aromatic and is used in medicine to cure rheumatism and
asthma.
Hiptage madablota (H. madablota) root is cultivated in India for medicinal purposes (Bailey
and Bailey 1976). Hiptage madablota is widely cultivated in the tropics for its attractive and
fragrant flowers; It is effect on Dosha: It pacifies Kapha and Vata.Traditionally, the root of H.
madablota used in the treatment of obesity and reduced the weight gain(Staples et al., 2000;
Whistler WA, 2000).
Whole plant of
Hiptage madablota Gaertn.
Root of
Hiptage madablota Gaertn.

6. Method:
Collection: The plant sample of Hiptage benghalensis was
collected in the form of leaf shavings. The collected plant
leaves were washed with water to rinse away undesirable
materials and plant parts. The cleansed leaves were then
partially dried by fan aeration and then fully dried in the oven
at below 40ºC.
Ethanolic Extract of Roots of Hiptage Madablota Gaertn
(EEHM): 103gram of powered material were placed in a
clean, flat bottomed glass container and soaked in 500 ml of
80%ethanol. The container was then sealed and subjected to
occasional shaking and stirring for a period of 2 days. After 48
hours the homogenous mixture was filtered first by cotton
material and twice through whatman filter paper to obtain a
finer filtrate. The filtrate (Ethanol extract) obtained was
evaporated by Rotary evaporator at 4 to 5 rpm and at 65ºc
temperature. The separated filtrate was found to be a
precipitate of dark green color and the gummy concentrate was
designated as the crude ethanol extract of the leaves of Hiptage
Benghalensis. It was then dried in the freeze drier and
preserved at +4ºC for two weeks

7. PHYTOCHEMICAL INVESTIGATION OF THE ROOT OFHIPTAGE
MADABLOTA GAERTN.
S.NO Phytoconstituents Ethanol Extract
1.
Alkaloids - ve
2.
Carbohydrates - ve
3.
Proteins +ve
4.
Terpenoids - ve
5. Phenolic compounds +ve
6. Flavonoids +ve
7. Tannins +ve
8. Glycosides +ve
9. Gums and mucilage - ve
10. Saponins +ve
11. Steroids - ve
12. Fixed oils & Fats - ve

8. INVITRO ANTIOXIDANT ACTIVITY OF ETHANOL EXTRACT OF ROOT OF
HIPTAGE MADABLOTA GAERTN.
The ethanol extract of Hiptage madablota L. root were investigated by using DPPH
scavenging test. The free radical scavenging activity of the EEHM was estimated by using
2, 2 Diphenyl-l-picrylhydrazyl radical (DPPH) using UV-Spectrometry (Blois, 1958) at
517 nm.
The DPPH solution was prepared in 95% ethanol. The EEHM was mixed with 95%
ethanol to prepare the stock solution in required concentration (10mg/100ml or
100µg/ml). From the stock solution 2ml, 4ml, 6ml, 8ml and 10ml of this solution were
taken by serial solution and were made the final volume of each test tube up to 10 ml
whose concentration was then 20µg/ml, 40µg/ml, 60µg/ml, 80µg/ml and 100 µg/ml
respectively. EEHM solution was prepared by serial dilution (20 µg/ml, 40µg/ml, 60
µg/ml, 80 µg/ml and 100 µg/ml) and after 10 min, the absorbance was taken at 517nm,
using a visible spectrophotometer. Ascorbic acid was used as a reference standard. 95%
methanol was used as blank .
% scavenging of the DPPH free radical was measured using following equation,
% of DPPH radical Scavenging activity = [(A0 - A1)/A0)] × 100
Where A0 is the absorbance of the control, and A1 is the absorbance of the extract and
standard.

9. S.
N
o
Conc.
(µg/ml)
Absorb of
ascorbic
acid (Ref)
%
scavenging
DPPH of
Ascorbic
acid (Ref)
Absorb of
EEHM
%
scavenging
DPPH of
EEHM
1 20µg/ml 0.146±0.02 37.19±0.27 0.132±0.02 43.27±0.21
2 40 µg/ml 0.104±0.01 55.27±0.21 0.092±0.01 60.37±0.13
3 60 µg/ml 0.085±0.02 63.4±0.1 0.072±0.01 68.9±0.1
4 80 µg/ml 0.059±0.01 74.55±0.05 0.044±0.02 81.04±0.01
5 100
µg/ml
0.012±0.01 94.82±0.01 0.017±0.02 92.6±0.1
Results:
EEHM exhibited a significant dose dependent inhibition of DPPH activity, the IC 50 value
of the EEHM and reference standard ascorbic acid were found to be 53.99µg/ml and
52.73µg/ml respectively.
EEHM exhibited the highest activity i.e., 92.60% whereas ascorbic acid exhibited 94.82%
( Table : DPPH radical scavenging activity of EEHM )
0
0.2
0.4
0.6
0.8
1
1.2
0 50 100 150
Absorbance
Concentration (µg/ml)
Absorbance of
Ascorbic acid
Absorbance of
EEHM

10. CONCLUSION
Hiptage madablota is cultivated for medicinal purposes and holds a reputed
position in Indian medicine. These results suggest that the presence of
Phenolic compounds and flavonoids present in EEHM will be the key
factor for the traditional uses of Hiptage Madablota Gaertn for various
diseases.

11. References:
1. Maheshwari P., Baburao B., Reddy Narsimha Rama. Hepatoprotective activity
of methanolic extract of Hiptage bengalensis leaves against CCl4-
induced hepatotoxicity in rats. Toxicology Mechanisms and Methods. 2012, Vol. 22,
No. 6, 483-487.
2. Winka Janorious J., Maithili V, Narayanan Jayaprakash J., Rajkumar M and
Kumar Senthil K .L.) Pharmacognostical and Priliminary Phytochemical Study
of Hiptage Benghalensis (L) KURZ. IOSR Journal of Pharmacy. 2012, Vol. 2(2):
170-179.
3. Akinmoladun, A.C., EO. Ibukun, E. A for EM. Obuotor and EO. Farombi.
Phytochemical constituent and antioxidant activity of extract from the leaves
of Ocimum gratissimum. Sci. Res. Essay 2007; 2: 163-166.
4. Chenthurpandy P, Kalidass C and Mohan V.R: Pharmacognostical Investigation
of Hiptage benghalensis (L.) Kurz. (Malpighiaceae). Pharmacognosy journal.
2010; 1(2).
5. Data Base on Medicinal Plants Used In Ayurveda and Siddha., Central Council
for Research in Ayurveda and Siddha, Dept. of AYUSH, Min. of Health and F.W.,
Govt. of India, Volume 3; 398-400.
6. Lalrotluanga, Ngente L, Nachimuthu SK, Guruswami G. Insecticidal and
repellent activity of Hiptage benghalensis L. Kruz (Malpighiaceae) against
mosquito vectors. Parasitol Res. 2012; 111(3):1007-1017.

12. THANK
YOU