The document outlines a study exploring how temperature affects the relationship between the enzyme catalase and its substrate hydrogen peroxide. Potato catalase and 3% hydrogen peroxide solution will be used in the experiment. Potato cubes will be placed in test tubes labeled for different temperatures (hot, cold, room temperature), then hydrogen peroxide will be added to observe the bubble production, measuring the reaction rate. The results will show how temperature impacts the activity of the catalase enzyme.

1. Write in the following in your notebook Title: The Properties of Enzymes: A Study of Catalase Purpose: Potato catalase and hydrogen peroxide will be used to explore the relationship between enzymes and substrates at various temperatures (maybe pH too). Procedures: You are going to write them down. Yay!

2. Enzyme-Substrate Complex

3. Denaturing Enzymes x x

4. What is Catalase Enzyme that breaks down hydrogen peroxide (H 2 O 2 ), which is a naturally occurring poison. The reaction catalyze by catalase is: 2H 2 O 2  2H 2 O + O 2 When you put hydrogen peroxide on a cut, what are the bubbles? Oxygen

5. Complete this sentence… The _____________ (more/less) enzyme, the faster the product is made.

6. The Procedure Part A: Label 3 large test tubes hot, cold and room temp. Cut 3 1.5cm 3 pieces of potato with peel removed. Use a mortar and pestle to macerate (mash/grind up) each cube. Place ALL of the macerated potato cubes into each labeled test tube.

7. 6. Add 1ml of distilled water to each test tube containing potato mash. 7. Place the test tube labeled hot in the hot water (100 o C) bath for 3 minutes. 8. Place the test tube labeled cold in the cold water bath (0-4 o C) for 3 minutes. 9. Leave the test tube labeled room temperature in the test tube rack. 10. After 3 minutes, remove the test tube from the baths and allow the hot test tube to cool.

8. 11. Add 5ml of H 2 O 2 (3.0% hydrogen peroxide) to each tube. 12. Wait one minute while reaction occurs then measure the height of the bubbles in each tube in cm. 13. Record in Table 1. Measure the radius of the test tube and record in Table 1. 14. Record your data in the class data table.

9. Teacher Role Student Role Safety Read procedure to know what to do next Follow the directions Stay on task Clean up Safety Have materials available