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Mathias Kinner1
, Susanne Siebenhandl-Ehn1
, Heinrich Grausgruber2
, Gabriele Kronawetter1
, Luis Pérez Berrocal1
, Tanja Gollinger1
, Philipp Wurbs1
, Michael Prückler1
, Emmerich Berghofer1
1
Department of Food Sciences and -technology and 2
Department of Applied Plant Sciences and Plant Biotechnology, University of Natural Resources and Life Sciences, Vienna Austria
AACC No
INTRODUCTION
Naked (Hordeum vulgare var. nudum) and hulled barley varieties share the same genetic background, except that the naked gene, nud, is expressed in the naked types. Naked barley
may be two- or six-rowed, have short or long awns, vary in straw height, and occur in hooded (awnless)-type barley. Environmental growing conditions have major influences on kernel
size and composition, but kernel size and shape are also genetically controlled characteristics (Newman and Newman, 2005).
In contrast to hulled barleys, the caryopsis threshes free of the hull in the manner of common wheat. With the removal of the hulls, nutrients in the caryopsis increase in relative proportion
because of reduction of fibre represented in the hulls (McGuire and Hockett, 1981). Naked barley is a good source of dietary fibre providing soluble and insoluble dietary fibre fractions
(Bhatty, 1999; Izydorczyk et al., 2000). Mixed-linkage (1→3),(1→4)-β-d-glucans (hereafter termed as β-glucans) are a major part of the soluble dietary fibre (SDF) in barley. Together with
arabinoxylans, a fraction of partly soluble nonstarch polysaccharides (NSP) occurring in the cell walls, β-glucans have a great impact on cereal processing and product properties.
The positive effects of β-glucans are mainly attributed to its high viscosity in aqueous solution and thus increasing the viscosity of the
contents within the intestinal tract (Jalili et al., 2000). Studies underline its short time blood sugar regulating effects (Cavallero et
al., 2002), health benefits for the cardiovascular system and weight management and helping maintain cholesterol levels
that are already within the normal range. The US Food and Drug Administration (FDA) approved the use of the health
claim for soluble fibre from certain foods and risk of coronary heart disease (21CFR101.81) for β-glucans-
containing food. In Europe, barley- and oat-containing products will be allowed to claim the
cholesterol lowering properties of β-glucans (EFSA, 2009).
AIMS and SCOPE
With the upcoming Health Claim
within the EC for beta-glucans to maintain
normal blood cholesterol concentrations, an
increased demand for these soluble cereal fibers is foreseen.
Bread, pasta and extrudates were used as model food to implement the
requested content of beta-glucans through naked barley flour fractions. The
influences of added naked barley flour fractions on the product specific physical
and sensory properties were investigated. The results showed that a replacement
of up to 80 % of wheat flour results in sensory palatable products.
Thus, it is possible to produce naked barley products with a relevant amount of beta-glucans.
Utilizing colored varieties, containing anthocyanins and phenolic acids may broaden the diversity
in nutrition and consequently add further health beneficial substances into staples.
METHODS
GRAIN SAMPLES
Four different coloured (black, purple, blue and yellow)
genotypes were used. The ß-glucans content ranged
between 5.7 – 6.5 mg/100 g middlings and was
determined according to the AACC method 32-23.
BREAD
Ingredients: barley middlings/wheat flour mix,
acidifier, pre-gelatinized wheat flour, malt flour,
margarine, dry yeast, sugar, salt and water.
Procedure: After mixing, the dough was proofed at
30°C and 85% rH in a proofer (G66W, MANZ,
Germany) and scaled thereafter to 400 g into the
baking tins and proofed again at the same conditions.
Finally, baking started at 230°C with top and bottom
heat for 35 min in a baking station (BS60/3W, MANZ,
Germany). The oven was steamed once after loading
at the maximum level and the temperature was
subsequently reduced to 180°C.
Literature:
Bhatty, R. S., 1999. The potential of hull-less barley. Cereal Chemistry 76, 589-599.
EFSA, 2009. Scientific Opinion on the substantiation of health claims related to beta-glucans and maintenance of normal blood
cholesterol concentrations (ID 754, 755, 757, 801, 1465, 2934) and maintenance or achievement of a normal body weight (ID 820,
823) pursuant to Article 13(1) of Regulation (EC) No 1924/2006. EFSA Journal 7, 1-18.
Izydorczyk, M. S. et al., 2000. Variation in total and soluble β-glucan content in hulless barley: effects of thermal, physical, and
enzymic treatments. Journal of Agricultural and Food Chemistry, 48, 982-989.
Jalili, T., Wildman, R.E.C., Medeiros, D.M., 2000. Nutraceutical roles of dietary fiber. Journal of Nutraceuticals, Functional and
Medical Foods 2 (4), 19-34.
McGuire, C.F., Hockett, E.A., 1981. Effect of awn length and naked caryopsis on malting quality of “Betzes” barley. Crop Science 21,
18-21.
Newman, C.W., Newman, R.K., 2005. Hulless barley for food and feed. In: Abdel-Aal E., Wood, P. (Eds.), Specialty grains for food
and feed. American Association of Cereal Chemists, Chapter 7.
Why is naked barley flour still hardly used for human consumption?
Because ...
… the availability and market of landraces is rather small.
… the knowledge related to the production, diversity and use is lost.
… the technological aspects as decreased water availability &
sparsely formed gluten network need adaptation.
… the sensory properties of the final products are different
compared to our common food.
Menu of the dayChoose one of the following dishes
and ingest your
daily portion of beta-glucans- 3g per day -according to the Health claim
proposed by EFSA
4 slices of bread
or100g of extrudatesor300g of fresh cooked noodles
Contact:
Susanne Siebenhandl-Ehn
University of Natural Resources and Life Sciences, Vienna
Muthgasse 18
1190 Vienna, Austria
e-mail: susanne.siebenhandl@boku.ac.at
EXTRUDATES
Ingredients: Barley middlings fractions and wheat
flour at a proportion of 50% in weight.
Procedure: The extruder used was a conical,
counter-rotating twin-screw hot-extruder (CM45-F,
Cincinnati Milacron, Austria) equipped with a matrix of
4 mm diameter and a screw of 1000 mm length and
90-45 mm diameter. Once the operating parameters
were achieved, the barley mixes were introduced
through the hopper and extrudates were cut with
knifes. Extruded samples were cooled down before
packing into plastic bags and storing at 20°C and
50% humidity.
PASTA
Ingredients: barley middlings, wheat semolina and
water.
Procedure: The final dough moisture was adjusted to
42%. The dough was transferred into the pasta press
(LaMonferina) and the pressing process was started.
The wet noodles were dried in a conditioning cabinet.
Once dry, the noodles were packed in bags and stored
at 20°C and 50% humidity before they were used for
further analysis.

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Poster_AACC_2010

  • 1. Mathias Kinner1 , Susanne Siebenhandl-Ehn1 , Heinrich Grausgruber2 , Gabriele Kronawetter1 , Luis Pérez Berrocal1 , Tanja Gollinger1 , Philipp Wurbs1 , Michael Prückler1 , Emmerich Berghofer1 1 Department of Food Sciences and -technology and 2 Department of Applied Plant Sciences and Plant Biotechnology, University of Natural Resources and Life Sciences, Vienna Austria AACC No INTRODUCTION Naked (Hordeum vulgare var. nudum) and hulled barley varieties share the same genetic background, except that the naked gene, nud, is expressed in the naked types. Naked barley may be two- or six-rowed, have short or long awns, vary in straw height, and occur in hooded (awnless)-type barley. Environmental growing conditions have major influences on kernel size and composition, but kernel size and shape are also genetically controlled characteristics (Newman and Newman, 2005). In contrast to hulled barleys, the caryopsis threshes free of the hull in the manner of common wheat. With the removal of the hulls, nutrients in the caryopsis increase in relative proportion because of reduction of fibre represented in the hulls (McGuire and Hockett, 1981). Naked barley is a good source of dietary fibre providing soluble and insoluble dietary fibre fractions (Bhatty, 1999; Izydorczyk et al., 2000). Mixed-linkage (1→3),(1→4)-β-d-glucans (hereafter termed as β-glucans) are a major part of the soluble dietary fibre (SDF) in barley. Together with arabinoxylans, a fraction of partly soluble nonstarch polysaccharides (NSP) occurring in the cell walls, β-glucans have a great impact on cereal processing and product properties. The positive effects of β-glucans are mainly attributed to its high viscosity in aqueous solution and thus increasing the viscosity of the contents within the intestinal tract (Jalili et al., 2000). Studies underline its short time blood sugar regulating effects (Cavallero et al., 2002), health benefits for the cardiovascular system and weight management and helping maintain cholesterol levels that are already within the normal range. The US Food and Drug Administration (FDA) approved the use of the health claim for soluble fibre from certain foods and risk of coronary heart disease (21CFR101.81) for β-glucans- containing food. In Europe, barley- and oat-containing products will be allowed to claim the cholesterol lowering properties of β-glucans (EFSA, 2009). AIMS and SCOPE With the upcoming Health Claim within the EC for beta-glucans to maintain normal blood cholesterol concentrations, an increased demand for these soluble cereal fibers is foreseen. Bread, pasta and extrudates were used as model food to implement the requested content of beta-glucans through naked barley flour fractions. The influences of added naked barley flour fractions on the product specific physical and sensory properties were investigated. The results showed that a replacement of up to 80 % of wheat flour results in sensory palatable products. Thus, it is possible to produce naked barley products with a relevant amount of beta-glucans. Utilizing colored varieties, containing anthocyanins and phenolic acids may broaden the diversity in nutrition and consequently add further health beneficial substances into staples. METHODS GRAIN SAMPLES Four different coloured (black, purple, blue and yellow) genotypes were used. The ß-glucans content ranged between 5.7 – 6.5 mg/100 g middlings and was determined according to the AACC method 32-23. BREAD Ingredients: barley middlings/wheat flour mix, acidifier, pre-gelatinized wheat flour, malt flour, margarine, dry yeast, sugar, salt and water. Procedure: After mixing, the dough was proofed at 30°C and 85% rH in a proofer (G66W, MANZ, Germany) and scaled thereafter to 400 g into the baking tins and proofed again at the same conditions. Finally, baking started at 230°C with top and bottom heat for 35 min in a baking station (BS60/3W, MANZ, Germany). The oven was steamed once after loading at the maximum level and the temperature was subsequently reduced to 180°C. Literature: Bhatty, R. S., 1999. The potential of hull-less barley. Cereal Chemistry 76, 589-599. EFSA, 2009. Scientific Opinion on the substantiation of health claims related to beta-glucans and maintenance of normal blood cholesterol concentrations (ID 754, 755, 757, 801, 1465, 2934) and maintenance or achievement of a normal body weight (ID 820, 823) pursuant to Article 13(1) of Regulation (EC) No 1924/2006. EFSA Journal 7, 1-18. Izydorczyk, M. S. et al., 2000. Variation in total and soluble β-glucan content in hulless barley: effects of thermal, physical, and enzymic treatments. Journal of Agricultural and Food Chemistry, 48, 982-989. Jalili, T., Wildman, R.E.C., Medeiros, D.M., 2000. Nutraceutical roles of dietary fiber. Journal of Nutraceuticals, Functional and Medical Foods 2 (4), 19-34. McGuire, C.F., Hockett, E.A., 1981. Effect of awn length and naked caryopsis on malting quality of “Betzes” barley. Crop Science 21, 18-21. Newman, C.W., Newman, R.K., 2005. Hulless barley for food and feed. In: Abdel-Aal E., Wood, P. (Eds.), Specialty grains for food and feed. American Association of Cereal Chemists, Chapter 7. Why is naked barley flour still hardly used for human consumption? Because ... … the availability and market of landraces is rather small. … the knowledge related to the production, diversity and use is lost. … the technological aspects as decreased water availability & sparsely formed gluten network need adaptation. … the sensory properties of the final products are different compared to our common food. Menu of the dayChoose one of the following dishes and ingest your daily portion of beta-glucans- 3g per day -according to the Health claim proposed by EFSA 4 slices of bread or100g of extrudatesor300g of fresh cooked noodles Contact: Susanne Siebenhandl-Ehn University of Natural Resources and Life Sciences, Vienna Muthgasse 18 1190 Vienna, Austria e-mail: susanne.siebenhandl@boku.ac.at EXTRUDATES Ingredients: Barley middlings fractions and wheat flour at a proportion of 50% in weight. Procedure: The extruder used was a conical, counter-rotating twin-screw hot-extruder (CM45-F, Cincinnati Milacron, Austria) equipped with a matrix of 4 mm diameter and a screw of 1000 mm length and 90-45 mm diameter. Once the operating parameters were achieved, the barley mixes were introduced through the hopper and extrudates were cut with knifes. Extruded samples were cooled down before packing into plastic bags and storing at 20°C and 50% humidity. PASTA Ingredients: barley middlings, wheat semolina and water. Procedure: The final dough moisture was adjusted to 42%. The dough was transferred into the pasta press (LaMonferina) and the pressing process was started. The wet noodles were dried in a conditioning cabinet. Once dry, the noodles were packed in bags and stored at 20°C and 50% humidity before they were used for further analysis.