Study of the physiological and genetic differences between the White collared flycatcher and Pied flycatcher of the genus Ficedula / M.V Drahulian, A.B Chaplygina, S.O. Kostenko, N.O.Savynska, P.P. Ostrovskyi, K.Yu.Gusar, Yu.V Grishenko // Conference: FEBS3+ Meeting - XI Parnas Conference - young scientistits forum "Biochemistry and Molecular Biology for Innovative Medicine" 3-5 September, 2018, Kyiv Ukraine. Ukr. Biochem. J., 2018. Vol. 90, Special Issue. P. 130. DOI: 10.13140/RG.2.2.29972.14723/1

1. STUDY OF THE PHYSIOLOGICAL AND GENETIC DIFFERENCES BETWEEN
THE WHITE COLLARED FLYCATCHER AND PIED FLYCATCHER OF THE GENUS FICEDULA
1Drahulian M.V., 2Chaplygina A.B., 1Kostenko S.O., 2Savynska N.O.,1Ostrovskyi P.P., 2Gusar K.Yu., 2Grishenko Yu.V.
1National University of Life and Environmental Sciences of Ukraine, Heroiv Oborony St., 15, 03041 Kyiv, Ukraine;
2 H.S. Skovoroda National Pedagogical University, Alchevskyh St., 29, 61002 Kharkiv, Ukraine
Fig.3. Screening of ISSR with
the primer (AGC) 6G: 1 -
marker, 2, 3,– Ficedula
albicollis; 4, 5 – Ficedula
hypoleuca. Electrophoresis
exposure time - 40 minutes of
exposure, 250 W.
Using of the primer (AGC)6G with
the DNA of the pied and white
collared flycatchers revealed quite
high level of genetic polymorphism.
Thus, a total sum of amplicons
formed in the PCR with the selected
primer ranged from 9 in the pied
flycatcher to 14 in the white collared
flycatcher. The length of amplicons
ranged between 270-1400 bp. A
majority of PCR-loci (83.33 % of the
total number of loci) received with
this primer were of average size.
Four PCR loci (16.67 %) were less
then 500 bp long. The spectra of
amplification products obtained with
the primer (AGC)6G showed that the
white-collared flycatchers had a more
heterogeneous structure.
Conclusions: The study of the leukogram, micronucleus test, and
the conduction of the ISSR analysis can be especially effective in the
study of intraspecies genetic differentiation.
Introduction and aim: The fact that the flycatchers are capable of interspecies hybridization is
one of a reason of particular interest to these species
Material and methods: We studied birds of the genus Ficedula (Ficedula albicollis and
Ficedula hypoleuca) to detect physiological and genetic differences between two species of
flycatchers. We took blood samples from the birds eye’s sinus in the protected area of
Homilshanski Forests, Kharkiv, Ukraine to study their leukogram patterns.
Results: We did not find any significant
difference in the leukogram between the
adults of two species. However, the
difference in the leukogram distribution was
found between the nestlings of white collared
and pied flycatchers. The heterophile
(granulocyte) indices were higher in the white
collared flycatcher nestlings by 2.96% (12.53
± 0.56) (p < 0.001), and those of lymphocytes
were higher in the pied flycatcher nestlings by
2.94% (86.0 ± 1.15) (p < 0.01). This may
indicate difference in the immune system
development, although, as mentioned above,
there was no particular leukogram difference
between the adults of two species. It seems
that with age the immune system of two
species is levelled and becomes similar. The
micronucleus test did not reveal any
significant difference between species and
age bird groups
3.46
0.73
0.46
3.14
1.71
1.0
0
0.5
1
1.5
2
2.5
3
3.5
4
eosinophils monocytes basophils
nestlings Ficedula
albicollis (n=15)
nestlings Ficedula
hypoleuca (n=7)
12.53
83.06
9.57
86.0
0
10
20
30
40
50
60
70
80
90
100
heterophiles lymphocytes
nestlings Ficedula
albicollis (n=15)
nestlings Ficedula
hypoleuca (n=7)
Fig. 1. Difference of indicators eosinophils, monocytes, basophils
between nestlings Ficedula albicollis and Ficedula hypoleuca
Fig. 2. Difference of indicators heterophiles and lympocytes
between nestlings Ficedula albicollis and Ficedula hypoleuca
The bird feathers were also collected for genetic
analysis to identify interspecies differences with
the application of the ISSR technique.