This document describes procedures for plant regeneration through callus culture using tobacco plants. It involves initiating callus cultures from tobacco explant tissue, observing the growth pattern of callus cultures over multiple phases, and inducing shoot and root organogenesis from the callus to regenerate tobacco plants. The objectives are to initiate and maintain callus cultures from tobacco explants, create cell suspension cultures, and induce organ development from callus to regenerate whole plants. Standard procedures are provided for callus initiation, maintenance, measurement of growth curves, and regeneration of shoots and roots. Results showed high rates of callus formation and organogenesis from explants and callus on optimal media formulations.