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Presented By
Sushil Kumar
Departement of Botany , Institute of Science
Banaras Hindu University
INTRODUCTION
Organisms that are free-floating or found in the water column (i.e. pelagic)
but are unable to swim against typical currents , generally small
• Neuston = plankton living at or just below the water surface
(e.g. Bacterial film)
• Pleuston = plankton that live at the surface but protrude into the air
(e.g. Portuguese Man O’War)
•Nekton = pelagic animals that are powerful enough swimmers to move at will
in the water column, under most conditions
(e.g. fish, dolphins, squid)
Benthos = organisms living on or
in the bottom (in the subtidal
zone)
Plankton, from Greek word for ‘drifter’ or ‘wanderer
Zooplankton
Phylum : Arthropoda
Subphylum: Crustacea
Cladocerans (4 orders)
• most with calcareous shell
(usually clear)
• Holoplankton
Polyphemus
Daphnia pulex
Zooplankton
Phylum: Arthropoda
Subphylum :Crustacea
• mostly marine, also few, terrestrial
•~50,000 spp.
Copepods – (> 10,000 spp.)
most abundant animals in oceans
mostly holoplankton (some parasitic)
swim with antennae, thoracic legs,
or mouthparts.
Candacia
Zooplankton
Phylum Nematoda (roundworms)
• small, unsegmented, smooth; bilateral symmetry
• mostly benthic
• move via ‘lashing’ movement (wiggling motion)
• no planktonic larvae
• ~12,000 spp.
Phytoplankton - Dinoflagellates
Kingdom Protista
Division Dinophyta (Gr. dinos: whirling; L. flagellum: whip)
(=Pyrrophyta) (Gr. pyros: fire, and phyton: plant)
– refers to bioluminescence of many forms
• most have 2 flagella
• may be armored (thecate)
or
Ceratium
Phytoplankton – Diatoms
•Pennate (L. pinnatus: feathered) – elongate; bilaterally symmetric
pinnate refers to markings on walls of some of these forms
Cymbella affinis
Pseudo-nitzschia
cleaned
frustule
of pennate
diatom
Why are plankton important?
 Important part of global carbon cycle
Food source (basis of the food web)
 Producer of oxygen (photosynthesis)
8
Plankton are an energy source for Marine
Ecosystems
 Many plankton are primary producers
Over 90 % of Marine Primary Production (energy produced) is from
Phytoplankton! The rest is from marine plants and other sources.
9
Satellite view: Map
Productivity in the
Ocean
Photo:NOAA
How are plankton studied?
 Collected with special nets
and sampling bottles
 Underwater cameras
 Microscopes
 Satellites
10
Plankton
collection
using Net in a
pond
Van Dorn Bottle
Do organisms spend their entire lives as
Plankton?
 Holoplankton spend
their entire life cycle as
plankton. i.e.-
Dinoflagellates, Diatoms
and Krill
 Meroplankton : spend
part of life as plankton i.e.
11
Diatom (Unicellular phytoplankton)
Photo: Rolf Gradinger, NOAA/OER
How are phytoplankton different from
zooplankton?
Phytoplankton
 Producers
 Single cells or chains of cells
including the smallest
plankton – picoplankton
(0.2 -2 microns)
 Remain near the surface
Zooplankton
 Consumers (including
herbivores and carnivores)
 Include microscopic and
macroscopic organisms
 May vertically migrate (to a
depth of 200m) during the
day for protection but
resurface at night to feed
12
Steps of Plankton isolation
 Selection of source:marine or fresh water body-(Sainta
Cruz Wetland)
 Collection : using nets
 Filtration - different sizes of filters / bolting cloth
 Planktons concentration
 Observation (preliminary)
 Staining(Rose bengal Dye)
 Observation under Stereo microscope/microscope
Planktons Counting Cell
Wiggling motion
Plankton Taxonomy
 Phytoplankton groups can be categorized
according to size and taxonomic features.
 Size category Linear size (µm)-
 Picoplankton - 0.2 – 2
 Nanoplankton - 2 - 20
 Microplankton - 20 - 200
 Macroplankton - > 200
Special Thanks to my Prof.
Sandeep Kumar Melhotra
Thank
You

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Plankton isolation / Classification

  • 1. Presented By Sushil Kumar Departement of Botany , Institute of Science Banaras Hindu University
  • 2. INTRODUCTION Organisms that are free-floating or found in the water column (i.e. pelagic) but are unable to swim against typical currents , generally small • Neuston = plankton living at or just below the water surface (e.g. Bacterial film) • Pleuston = plankton that live at the surface but protrude into the air (e.g. Portuguese Man O’War) •Nekton = pelagic animals that are powerful enough swimmers to move at will in the water column, under most conditions (e.g. fish, dolphins, squid) Benthos = organisms living on or in the bottom (in the subtidal zone) Plankton, from Greek word for ‘drifter’ or ‘wanderer
  • 3. Zooplankton Phylum : Arthropoda Subphylum: Crustacea Cladocerans (4 orders) • most with calcareous shell (usually clear) • Holoplankton Polyphemus Daphnia pulex
  • 4. Zooplankton Phylum: Arthropoda Subphylum :Crustacea • mostly marine, also few, terrestrial •~50,000 spp. Copepods – (> 10,000 spp.) most abundant animals in oceans mostly holoplankton (some parasitic) swim with antennae, thoracic legs, or mouthparts. Candacia
  • 5. Zooplankton Phylum Nematoda (roundworms) • small, unsegmented, smooth; bilateral symmetry • mostly benthic • move via ‘lashing’ movement (wiggling motion) • no planktonic larvae • ~12,000 spp.
  • 6. Phytoplankton - Dinoflagellates Kingdom Protista Division Dinophyta (Gr. dinos: whirling; L. flagellum: whip) (=Pyrrophyta) (Gr. pyros: fire, and phyton: plant) – refers to bioluminescence of many forms • most have 2 flagella • may be armored (thecate) or Ceratium
  • 7. Phytoplankton – Diatoms •Pennate (L. pinnatus: feathered) – elongate; bilaterally symmetric pinnate refers to markings on walls of some of these forms Cymbella affinis Pseudo-nitzschia cleaned frustule of pennate diatom
  • 8. Why are plankton important?  Important part of global carbon cycle Food source (basis of the food web)  Producer of oxygen (photosynthesis) 8
  • 9. Plankton are an energy source for Marine Ecosystems  Many plankton are primary producers Over 90 % of Marine Primary Production (energy produced) is from Phytoplankton! The rest is from marine plants and other sources. 9 Satellite view: Map Productivity in the Ocean Photo:NOAA
  • 10. How are plankton studied?  Collected with special nets and sampling bottles  Underwater cameras  Microscopes  Satellites 10 Plankton collection using Net in a pond Van Dorn Bottle
  • 11. Do organisms spend their entire lives as Plankton?  Holoplankton spend their entire life cycle as plankton. i.e.- Dinoflagellates, Diatoms and Krill  Meroplankton : spend part of life as plankton i.e. 11 Diatom (Unicellular phytoplankton) Photo: Rolf Gradinger, NOAA/OER
  • 12. How are phytoplankton different from zooplankton? Phytoplankton  Producers  Single cells or chains of cells including the smallest plankton – picoplankton (0.2 -2 microns)  Remain near the surface Zooplankton  Consumers (including herbivores and carnivores)  Include microscopic and macroscopic organisms  May vertically migrate (to a depth of 200m) during the day for protection but resurface at night to feed 12
  • 13. Steps of Plankton isolation  Selection of source:marine or fresh water body-(Sainta Cruz Wetland)  Collection : using nets  Filtration - different sizes of filters / bolting cloth  Planktons concentration  Observation (preliminary)  Staining(Rose bengal Dye)  Observation under Stereo microscope/microscope
  • 15. Plankton Taxonomy  Phytoplankton groups can be categorized according to size and taxonomic features.  Size category Linear size (µm)-  Picoplankton - 0.2 – 2  Nanoplankton - 2 - 20  Microplankton - 20 - 200  Macroplankton - > 200
  • 16. Special Thanks to my Prof. Sandeep Kumar Melhotra