2. Group Members
•Adan Fatima Javed
•Sehrish Shoukat
•Zunaira Khalil
•Basharat Khan
•Syed Haider Ali
•Salimullah Khan Ayubi

4. Pesticides
Pesticides are substances meant for
preventing, destroying or mitigating
any pest.

5. Types of pesticides/Classes breakup
2%
Insecticides
9%
Herbicides
14%
Fungicides
75%
Acricides

6. Why Pesticides are used?
• Population pressure
• Food security
• Higher yield

7. • Province wise distribution
KPK 2%
Sindh 8%
Punjab
90%

8. Toxicity potential of Pesticides
Direct impact on humans
• Adversely impact on
Central Nervous
System (CNS)
• Toxic

9. Fate of Pesticide in Environment

10. Profenofos

11. Selected Pesticide-Profenofos
• Widely used in Pakistan
• Hazardous to health

12. Reasons
• Among top ten
used pesticides in
Pakistan
– Used on cotton
– Commonly found
as groundwater
contaminants
– More effective

13. Biodegradation
• Microorganisms
• Use compound as carbon and energy
source

14. Methodology

15. Methodology Steps
Sampling
Enrichment
Incubation
Spreading
Plate Count
Isolation
Acclimatization
Serial Dilutions
Characterization

16. Media
Mineral Salt Media (MSM)
Use Distilled Water
For 100 ml of media
(0.15g/100 ml)

17. Autoclave
•Media
•Glass wares
e.g petri plates
etc….

18. Enrichment
Soil Sample
+
Media
Orbital Shaker
For 2 Days

19. Acclimatization
1 ml
Inoculum
Media
100 ml
100 ml
Concentration
of
profenofos
control
50 ppm
1 ml
100 ml
100 ppm
1 ml
100 ml
150 ppm

22. Incubation
 For 2 days (48 hours)
at 37 oC.

23. Plate Count

24. Characterization
•Morphological Characterization
 Colony Morphology (Microscopy)
 Cell Morphology (Through staining)
•Physiological Characterization
•Biochemical Characterization
•Molecular Characterization

25. Conclusion
•Specific growth of pesticide degrading microorganisms
•Planning for isolation and Characterization in the next step

26. Experience
•Very good experience
•Applications of Microbiology in Environmental
Engineering
•Would like to continue the research in the next
semesters

27. Facilities in IESE Microbiology Lab
•Sterile Environment
•Well equipped
•Very cooperative and trained Staff
•Students/Researchers are always welcomed

28. Suggestions
•
Separate lab for Bioremediation
• HEPA Filters

29. Acknowledgement


Professor Dr. Imran Hashmi
Miss Arish Naseem