The document summarizes a genetic transformation workshop where students learned how to transform bacteria by adding exogenous DNA. They used a plasmid containing the gene for green fluorescent protein (GFP) to transform bacterial colonies. The transformed bacteria were plated on selective media where only colonies containing the GFP plasmid would grow over the course of a week, demonstrating successful genetic transformation.

1. José Gerardo Cruz Rivera804-10-1716<br />pGLO Bacterial<br />Transformation<br />The workshop given by Dra. Belinda Roman was about how to perform a procedure known as genetic transformation. This genetic transformation is the genetic alteration of a cell resulting from the direct uptake, incorporation, and expression of exogenous genetic material (exogenous DNA) from its surrounding and taken up through the cell membrane(s). Transformation occurs most commonly in bacteria, and in some species it occurs naturally. Also, we used a procedure to transform bacteria within genes that code for Green Fluorescent Protein (GFP). GFP is a protein composed of 238 amino acid residues (26.9kDa) that exhibits bright green fluorescence when exposed to blue light. In this part of the lab, we added a transformation solution to tubes identified as +pGlo and -PGlo. Then, we added colonies of bacteria to +pGlo and –Pglo. In + PGLO, we only added a plasmid DNA. After hot water baths and adding LB broth to both tubes, we added + PGLO to transformation plates and -pGlo to control plates. At the end of the week, we would observe in which plates colonies grew.<br />