This study aimed to introduce the green fluorescent protein (GFP) gene into E. coli and human cells. GFP was successfully inserted into E. coli and shown to be expressed under control of the L-arabinose promoter. Addition of restriction sites to GFP was also successful, allowing for digestion of the GFP and pcDNA plasmid. However, ligation of the digested GFP fragment into pcDNA was unsuccessful, likely due to nuclease contamination. Expression of GFP in human cells could not be verified due to a technical error during immunoblotting. While some goals were achieved, such as GFP expression in E. coli, further optimization is needed to fully introduce GFP into human cells via this methodology.