1. The document describes an experiment to isolate plasmid DNA from E. coli bacteria transformed with the pGLO plasmid. 2. The plasmid DNA was isolated using a modified alkaline lysis method. Samples of the isolated plasmid DNA were run on a gel electrophoresis along with a ladder for comparison but the ladder showed an unusual single band. 3. While single bands were observed for the plasmid DNA samples, the abnormal ladder prevented further analysis of the isolated plasmid DNA.