This document provides an overview of Scintica's preclinical imaging product portfolio, including their technical capabilities. It describes several of Scintica's imaging systems - the Prospect T1 compact ultrasound system, M-Series compact MRI systems, FIVE2 fluorescence endomicroscopy, Newton 7.0 optical imaging system, and SuperArgus PET/CT systems. Sample images from live demonstrations of the Prospect T1, M-Series, and Newton 7.0 are also presented to showcase their imaging capabilities.
How high frequency ultrasound imaging is supporting preclinical research appl...Scintica Instrumentation
This free webinar hosted by Scintica Instrumentation introduced participants to some of the basics of high frequency ultrasound imaging and reviewed the most common preclinical research applications for this exciting technology. Our presenter, Ms. Tonya Coulthard, discussed the basic principles of ultrasound imaging, presented sample images from various organs and tissues as a way of surveying common research applications, and introduced a few novel techniques that will be of interest to many researchers. Throughout the webinar, technical information, product specifications and sample images where shown from the Prospect T1 compact, high-frequency, preclinical ultrasound imaging system.
Recent advances in MRI technology include faster scan times through simultaneous multi-slice imaging and automated brain scans. Lung MRI is now possible using new sequences. Cardiac MRI has been simplified through automated full-volume scans. Software guides scans for patients with MR-conditional implants. The first 7T MRI system was approved for clinical use. New MRI systems have entered the market with upgrades like ambient lighting experiences to reduce patient anxiety.
This document discusses various types of medical imaging technologies. It describes radiologic/x-ray technology, ultrasound technology, CT scans, MRI scans, and nuclear imaging including PET and SPECT. The goal of medical imaging is to non-invasively examine the inside of the body to diagnose health problems and guide treatment. Each technology has advantages for certain applications based on the type of information and depth of imaging it provides. Together these modalities provide physicians a variety of tools to accurately diagnose and monitor patient health issues.
The document discusses general-purpose ultrasound scanners used in hospitals. It describes the key components of an ultrasound system including the transducer probe, ultrasound monitor, and image storage system. It explains the imaging principles of ultrasound, how ultrasound waves are transmitted and reflected at tissue interfaces depending on acoustic impedance, and how the reflected echoes are used to generate images. It also outlines different types of ultrasound scanners and transducers as well as imaging modes.
Radiology uses medical imaging to diagnose and sometimes treat diseases. Modalities include X-ray, ultrasound, CT, nuclear medicine including PET, and MRI. Interventional radiology uses imaging to guide minimally invasive procedures. Plain radiography is commonly used for initial assessment but has lower sensitivity than newer modalities. It is useful for visualizing bone tumors, fractures, and arthritis. Ultrasound uses sound waves to image soft tissues in real time without radiation. CT obtains 3D images but has disadvantages of cost and radiation exposure. MRI provides high soft tissue contrast images in multiple planes but has contraindications for patients with metallic implants. Nuclear medicine involves radioactive tracers that accumulate in tissues to evaluate physiological function.
The document discusses the main components of a CT scanner system. It describes the key components as including the x-ray source, high-powered generator, detector, data transmission systems, and computer system for image reconstruction. It provides details on the gantry, detectors, data acquisition system, slip-ring technology that allows continuous rotation, and operating console as the main control center.
This document discusses analyzing PHANTOM images to determine the reliability of PET/SPECT cameras. It begins with introducing nuclear medicine imaging techniques like PET and SPECT that use gamma cameras. The goal is to automate the quality assurance testing procedure for these cameras to reduce the time from over 4 hours to less. The document then covers the background theory on image registration and marker detection algorithms that could be used. It outlines the software requirements, structure, and testing plans. It concludes with discussing simulations run to solve problems in working with DICOM images, mask creation, finding best slices, and more to optimize the automated QA procedure.
How high frequency ultrasound imaging is supporting preclinical research appl...Scintica Instrumentation
This free webinar hosted by Scintica Instrumentation introduced participants to some of the basics of high frequency ultrasound imaging and reviewed the most common preclinical research applications for this exciting technology. Our presenter, Ms. Tonya Coulthard, discussed the basic principles of ultrasound imaging, presented sample images from various organs and tissues as a way of surveying common research applications, and introduced a few novel techniques that will be of interest to many researchers. Throughout the webinar, technical information, product specifications and sample images where shown from the Prospect T1 compact, high-frequency, preclinical ultrasound imaging system.
Recent advances in MRI technology include faster scan times through simultaneous multi-slice imaging and automated brain scans. Lung MRI is now possible using new sequences. Cardiac MRI has been simplified through automated full-volume scans. Software guides scans for patients with MR-conditional implants. The first 7T MRI system was approved for clinical use. New MRI systems have entered the market with upgrades like ambient lighting experiences to reduce patient anxiety.
This document discusses various types of medical imaging technologies. It describes radiologic/x-ray technology, ultrasound technology, CT scans, MRI scans, and nuclear imaging including PET and SPECT. The goal of medical imaging is to non-invasively examine the inside of the body to diagnose health problems and guide treatment. Each technology has advantages for certain applications based on the type of information and depth of imaging it provides. Together these modalities provide physicians a variety of tools to accurately diagnose and monitor patient health issues.
The document discusses general-purpose ultrasound scanners used in hospitals. It describes the key components of an ultrasound system including the transducer probe, ultrasound monitor, and image storage system. It explains the imaging principles of ultrasound, how ultrasound waves are transmitted and reflected at tissue interfaces depending on acoustic impedance, and how the reflected echoes are used to generate images. It also outlines different types of ultrasound scanners and transducers as well as imaging modes.
Radiology uses medical imaging to diagnose and sometimes treat diseases. Modalities include X-ray, ultrasound, CT, nuclear medicine including PET, and MRI. Interventional radiology uses imaging to guide minimally invasive procedures. Plain radiography is commonly used for initial assessment but has lower sensitivity than newer modalities. It is useful for visualizing bone tumors, fractures, and arthritis. Ultrasound uses sound waves to image soft tissues in real time without radiation. CT obtains 3D images but has disadvantages of cost and radiation exposure. MRI provides high soft tissue contrast images in multiple planes but has contraindications for patients with metallic implants. Nuclear medicine involves radioactive tracers that accumulate in tissues to evaluate physiological function.
The document discusses the main components of a CT scanner system. It describes the key components as including the x-ray source, high-powered generator, detector, data transmission systems, and computer system for image reconstruction. It provides details on the gantry, detectors, data acquisition system, slip-ring technology that allows continuous rotation, and operating console as the main control center.
This document discusses analyzing PHANTOM images to determine the reliability of PET/SPECT cameras. It begins with introducing nuclear medicine imaging techniques like PET and SPECT that use gamma cameras. The goal is to automate the quality assurance testing procedure for these cameras to reduce the time from over 4 hours to less. The document then covers the background theory on image registration and marker detection algorithms that could be used. It outlines the software requirements, structure, and testing plans. It concludes with discussing simulations run to solve problems in working with DICOM images, mask creation, finding best slices, and more to optimize the automated QA procedure.
LIVE DEMONSTRATION: Understanding the Complimentary Nature of BLI, Ultrasound...Scintica Instrumentation
This will be a full day of live imaging where we will be examining the complimentary nature of three preclinical imaging modalities – bioluminescence, high-frequency ultrasound, and MRI. We will image the same tumor models on each of the imaging modalities; but will also have a normal mouse available to look at cardiovascular function using ultrasound, and brain anatomy using MRI.
Changing how researchers think about MRI: Utilizing a simple to use, compact...Scintica Instrumentation
In this webinar Tonya Coulthard discussed features and benefits of the M-Series product line, focusing on the unique self-shielded compact design of the systems which allows them to be placed in any existing laboratory or animal facility right next to existing instrumentation or fixtures, without the need for added infrastructure, plumbing or cryogens. Ms. Coulthard highlighted the intuitive software interface, and optimized default sequences, which allow users to acquire high resolution anatomical, functional, and molecular images without any prior experience in MR imaging. Key research applications and example images were also reviewed.
Hands-on demonstration of the Prospect T1, high-frequency ultrasound system
During this live demonstration we demonstrated the animal preparation steps and positioning for both mice and rats on the Prospect T1. We also showed the following cardiac views on both species:
Long Axis, in B-Mode
Short Axis, in B-Mode and M-Mode
Pulmonary Artery, in B-Mode, Color Doppler, Pulsed Wave Doppler
Apical 4-Chamber to Measure Mitral Valve Flow, in B-Mode, Color Doppler, Pulsed Wave Doppler, and Tissue Doppler
We also examined the aortic arch, carotid arteries, abdominal aorta, and other abdominal organs throughout the demonstration.
(May 17, 2021) Introducing the Newton 7.0 Optical Imaging System: The Modalit...Scintica Instrumentation
In this webinar, Katie reviewed how optical imaging works and provided some common examples of how it’s used within preclinical research. She introduced the Newton 7.0 FT500, an optical imaging system manufactured by Vilber that included bioluminescence, fluorescence, and 3D tomography capabilities. She concluded the webinar by going through some of the most common questions/considerations that come up for those looking to add optical imaging capabilities to their lab.
The Newton 7.0 is a highly sensitive optical imaging system dedicated to preclinical imaging of small animals in vivo, and may also be used on a variety of in vitro and ex vivo samples. It combines the best optics and animal handling features for high-quality image data and quantification. The Newton 7.0 system is capable of bioluminescence, fluorescence as well as 3D tomographic imaging.
The system is:
User-friendly
Does not require any radiation to acquire images
Is non-invasive, allowing for longitudinal studies
Allows for up to 5 mice or 3 rats to be imaged simultaneously
The Newton 7.0 is highly sensitive and can be used in a wide variety of research applications. Various bioluminescent reporters like firefly luciferase and many fluorescent molecular reagents can be used to visualize and track tumors, monitor disease or inflammation development, target molecules to nanoparticles or follow biodistribution and pharmacokinetics noninvasively.
Whether you are just exploring the idea of adding optical capabilities to your lab or you’ve been imaging for years, you won’t want to miss the opportunity to learn about this cool new technology and ask questions about your specific research applications and study design.
Learning objectives:
How does optical imaging work?
Common applications of preclinical optical imaging
A product overview: Newton 7.0 FT500
What makes the system unique?
How to add optical imaging capabilities to your lab?
In collaboration with The Keenan Research Center for Biomedical Science, Toronto
Expanding preclinical and histopathology capabilities with MRI technology: a ...Scintica Instrumentation
This free webinar hosted by Scintica Instrumentation reviewed the fundamentals of Magnetic Resonance Histology (MRH) and provided a number of relevant examples. Magnetic Resonance Imaging (MRI) has been used for years in preclinical research to perform in vivo studies allowing for the sensitive detection of pathological changes in soft tissue and to provide quantitative three-dimensional data.It has been used in longitudinal studies to noninvasively monitor the genesis, progression and regression of a wide variety of diseases, reducing the need for interim sacrifice of animals at specified time points, thus allowing the same animal to be used as its own control within a given study. MRH is the use of MR imaging on formalin-fixed tissues for high resolution characterization of tissue structure. It is a highly valuable complimentary adjunct to conventional histopathology, as it permits a thorough examination to be performed through multiple digital slices of an entire organ, while leaving the formalin-fixed specimen intact for subsequent definitive conventional diagnostic histopathology.
Optimal fuzzy rule based pulmonary nodule detectionWookjin Choi
The document describes a lung cancer detection system that uses CT scans. It discusses (1) segmenting the lungs from CT images using adaptive thresholding and connected component analysis, (2) detecting nodule candidate regions using multi-thresholding and rule-based pruning, and (3) optimizing the rule-based pruning using a genetic algorithm trained fuzzy inference system to reduce false positives while maintaining high sensitivity. Experimental results on a publicly available lung image database show the optimized fuzzy system achieved better performance than a conventional rule-based approach.
PET scan uses radiotracers like FDG to produce 3D images showing biochemical functioning in the body. It has several components including detectors that detect gamma rays emitted by the radiotracer and a computer that analyzes the data to create images. PET scans are used to detect cancer, determine if cancer has spread, evaluate brain abnormalities, and more.
This document discusses various imaging techniques used in veterinary medicine, including fiberoptic endoscopy, computed tomography, magnetic resonance imaging, nuclear medicine techniques like scintigraphy, and fluoroscopy. It provides information on the principles, equipment, clinical applications, and procedures for each technique. Key points covered include how endoscopy can be used to evaluate the gastrointestinal tract, the physics behind computed tomography image formation using x-rays, the use of radioisotopes and radiopharmaceuticals in scintigraphy, and safety considerations for fluoroscopy due to radiation exposure.
This document discusses the basics of CT scanning, including its history and key components. It describes how CT scanning works, from the x-ray tube emitting radiation that is detected after passing through the body, to the computer using this data to reconstruct cross-sectional images. It outlines the main parts of a CT system, including the gantry, detector, and control console. It also explains different scanning methods and how image quality is determined.
Invasive Blood Pressure: fundamentals and best-practices for preclinical rese...InsideScientific
In this webinar sponsored by ADInstruments, Tom Smith, Professor of Orthopaedic Surgery at Wake Forest School of Medicine, and expert in microsurgery and vascular pressure measurement techniques discusses the fundamental properties of pressure measurements in the vascular system, including the history and physics behind solid-state manometry. In addition, he explains the importance and impact of high fidelity solid-state catheters as it relates to accuracy, consistency and research outcomes.
The SuperArgus state-of-the-art preclinical PET/CT system: An overview of the...Scintica Instrumentation
These systems are ideally suited for pre-clinical imaging of small animals such as mice and rats, all the way up to medium sized animals such as rabbits, non-human primates and other similarly sized animals. Some of the unique imaging capabilities include real-time imaging of awake animals, as well as multiplexed PET imaging of standard and non-standard isotopes. Key research applications and example images were reviewed.
Positron Emission Tomography (PET) is the gold standard in metabolic imaging, providing high sensitivity to specific radiotracer used to detect specific metabolic activity or biomarkers in vivo. The most common uses for PET imaging in pre-clinical research include oncology, neurobiology, cardiology, as well as dynamic imaging.
These systems are considered to be best in class imaging system with state of the art detectors and electronics. The systems have been designed to be self-shielded, requiring no additional shielding at the location selected for installation. The systems come in a three different bore sizes allowing for imaging of animals such as mice all the way up to rabbits and even non-human primates. The CT component of these systems has been optimized for reduced radiation exposure, rapid acquisition times, and high resolution images; all ideal for the longitudinal studies so commonly performed in pre-clinical research.
The SuperArgus system is uniquely designed to provide consistent resolution across the entire field of view, while maintaining sensitivity and system performance. Reconstruction algorithms have also been implemented to rapidly process and display the acquired images. The system performs very well for standard imaging applications such as oncology, cardiology, etc. Additionally, the system has some unique features which allow for some unique imaging capabilities such as real-time awake animal imaging, self-gated cardiac imaging, as well as multiplex imaging of standard and non-standard isotopes.
This document discusses the use of computers in veterinary surgery and medicine. It outlines the history of computers from Charles Babbage's concept in the 1830s to their use in veterinary science in the 1980s. Computers can be used as virtual labs to model drug effects, as simulators for surgical and medical training, and for data management in veterinary hospitals. They also assist with diagnosis, developing treatment plans, education, and various imaging and surgical techniques like digital radiography, ultrasound, CT scans, and MRI.
The document describes the IVM-MS intravital microscopy system from Scintica. The system provides an all-in-one solution for intravital imaging of various organs in live animals. It integrates hardware and software optimized for high-quality intravital imaging. The system includes components for animal maintenance during imaging and can perform functions like z-stack, mosaic, and time-lapse imaging. Examples shown include brain, tumor, lung, and heart imaging applications in window chamber models using confocal and two-photon microscopy.
Ultrasound Machine-A Revolution In Medical ImagingRAVI KANT
What is medical imaging?
Why ultrasound imaging is required?
History of ultrasound
What is ultrasound
Physical definition
Medical definition
Ultrasound production
The Returning echo
Doppler effect
What is Doppler ultrasound
Principles of instrumentation in ultrasonography
Transmitter and receiver circuits of ultrasound
Mechanical assembly of ultrasound machine
Manufacturing companies of USG
Sonoscape S40 color Doppler ultrasound system
Clinical applications of ultrasound
Future of ultraso
The document discusses problems caused by respiratory motion during radiotherapy treatment planning and delivery. It describes limitations in image acquisition, treatment planning, and radiation delivery due to organ motion. It then outlines several methods to account for respiratory motion, including motion encompassing techniques like slow CT, inhale/exhale breath-hold CT, and 4D CT. Respiratory gating techniques using external markers like the Varian Real-time Position Management (RPM) system or internal markers are also summarized. The RPM system and process for using external markers for respiratory gated imaging and treatment are described in detail.
Dr. Lawrence Yip explained how Photoacoustic (PA) imaging works, where it fits in with other modalities and, how your research could benefit from this emerging technology.
Excellent spatial resolution, depth penetration, and superior contrast are just some of the advantages often associated with PA imaging. In this webinar, we dove into the advantages, where they can be beneficial, and how the TriTom’s patented technology overcomes some of the challenges experienced by early adopters of this imaging modality.
The TriTom is a turnkey, compact, tabletop imaging system that combines the sensitivity of fluorescence molecular tomography with the depth penetration and spatial resolution of PA tomography. Many applications including cancer, neuroimaging, developmental biology, and cardiovascular research could benefit from adding these imaging modalities, and we will draw from literature and concrete examples to demonstrate this advantage.
Freedom: The Promise of Telemetry Revisited - Stellar Telemetry Webinar (TSE ...InsideScientific
The innovative Stellar Telemetry System allows individual recording of key physiological parameters in freely roaming and socially active animals. In this webinar we will discuss added capabilities of such a system, in particular flexibility and freedom for both scientist and subject. In one 'subtropical' setting we will learn how such a system can be used in a colony of primates that are free to roam, without traditional geographical or data management limitations. In the other setting we return to the lab and see how a system like this can enhance measurement capabilities in a traditional rodent model.
Lecture 3 & 4 anam sanam chick ldkfdlsfldfjdlsjfdlks .pptxfaiz3334
Computed tomography (CT) scans create cross-sectional images of the body by using X-rays and computer processing. An X-ray tube rotates around the body and produces multiple images from different angles, which are used to reconstruct cross-sectional slices using back projection. These slices can be combined to create 3D images. CT scans provide more detailed images than basic X-rays due to their ability to distinguish between different tissue densities and visualize structures throughout the body.
CT or CAT scanners use X-rays and computer technology to create cross-sectional images of the body. Godfrey Hounsfield invented the first commercially viable CT scanner in 1967. A CT scanner is composed of a gantry with an X-ray tube that rotates around the patient, detectors, and a data system. X-rays pass through the body and are measured by detectors. A computer uses reconstruction algorithms to generate images from the data. CT scans provide detailed images and have advantages like detecting multiple types of tissues, being fast and painless, and helping diagnose many conditions.
(March 13, 2024) Overview of Preclinical Small Animal and Multimodal ImagingScintica Instrumentation
In this webinar, we reviewed some of the most commonly used preclinical imaging modalities, including magnetic resonance imaging (MRI), positron emission tomography (PET), computer tomography (CT), ultrasound, photoacoustic, bioluminescence, fluorescence, dual-energy x-ray absorptiometry (DEXA/DXA), and intravital microscopy. For each modality, we spent time reviewing the basics of how each worked, the strengths and considerations of each, and some key application areas and example images. Finally, we discussed the benefits of multimodal imaging and reviewed a few papers utilizing a variety of imaging modalities to help support their research outcomes.
We ended with a very brief introduction to Scintica Instrumentation and our philosophy behind the various products we represented. However, the main focus of the webinar was on education, and not our diverse product portfolio.
(June 12, 2024) Webinar: Development of PET theranostics targeting the molecu...Scintica Instrumentation
Targeting Hsp90 and its pathogen Orthologs with Tethered Inhibitors as a Diagnostic and Therapeutic Strategy for cancer and infectious diseases with Dr. Timothy Haystead.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
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LIVE DEMONSTRATION: Understanding the Complimentary Nature of BLI, Ultrasound...Scintica Instrumentation
This will be a full day of live imaging where we will be examining the complimentary nature of three preclinical imaging modalities – bioluminescence, high-frequency ultrasound, and MRI. We will image the same tumor models on each of the imaging modalities; but will also have a normal mouse available to look at cardiovascular function using ultrasound, and brain anatomy using MRI.
Changing how researchers think about MRI: Utilizing a simple to use, compact...Scintica Instrumentation
In this webinar Tonya Coulthard discussed features and benefits of the M-Series product line, focusing on the unique self-shielded compact design of the systems which allows them to be placed in any existing laboratory or animal facility right next to existing instrumentation or fixtures, without the need for added infrastructure, plumbing or cryogens. Ms. Coulthard highlighted the intuitive software interface, and optimized default sequences, which allow users to acquire high resolution anatomical, functional, and molecular images without any prior experience in MR imaging. Key research applications and example images were also reviewed.
Hands-on demonstration of the Prospect T1, high-frequency ultrasound system
During this live demonstration we demonstrated the animal preparation steps and positioning for both mice and rats on the Prospect T1. We also showed the following cardiac views on both species:
Long Axis, in B-Mode
Short Axis, in B-Mode and M-Mode
Pulmonary Artery, in B-Mode, Color Doppler, Pulsed Wave Doppler
Apical 4-Chamber to Measure Mitral Valve Flow, in B-Mode, Color Doppler, Pulsed Wave Doppler, and Tissue Doppler
We also examined the aortic arch, carotid arteries, abdominal aorta, and other abdominal organs throughout the demonstration.
(May 17, 2021) Introducing the Newton 7.0 Optical Imaging System: The Modalit...Scintica Instrumentation
In this webinar, Katie reviewed how optical imaging works and provided some common examples of how it’s used within preclinical research. She introduced the Newton 7.0 FT500, an optical imaging system manufactured by Vilber that included bioluminescence, fluorescence, and 3D tomography capabilities. She concluded the webinar by going through some of the most common questions/considerations that come up for those looking to add optical imaging capabilities to their lab.
The Newton 7.0 is a highly sensitive optical imaging system dedicated to preclinical imaging of small animals in vivo, and may also be used on a variety of in vitro and ex vivo samples. It combines the best optics and animal handling features for high-quality image data and quantification. The Newton 7.0 system is capable of bioluminescence, fluorescence as well as 3D tomographic imaging.
The system is:
User-friendly
Does not require any radiation to acquire images
Is non-invasive, allowing for longitudinal studies
Allows for up to 5 mice or 3 rats to be imaged simultaneously
The Newton 7.0 is highly sensitive and can be used in a wide variety of research applications. Various bioluminescent reporters like firefly luciferase and many fluorescent molecular reagents can be used to visualize and track tumors, monitor disease or inflammation development, target molecules to nanoparticles or follow biodistribution and pharmacokinetics noninvasively.
Whether you are just exploring the idea of adding optical capabilities to your lab or you’ve been imaging for years, you won’t want to miss the opportunity to learn about this cool new technology and ask questions about your specific research applications and study design.
Learning objectives:
How does optical imaging work?
Common applications of preclinical optical imaging
A product overview: Newton 7.0 FT500
What makes the system unique?
How to add optical imaging capabilities to your lab?
In collaboration with The Keenan Research Center for Biomedical Science, Toronto
Expanding preclinical and histopathology capabilities with MRI technology: a ...Scintica Instrumentation
This free webinar hosted by Scintica Instrumentation reviewed the fundamentals of Magnetic Resonance Histology (MRH) and provided a number of relevant examples. Magnetic Resonance Imaging (MRI) has been used for years in preclinical research to perform in vivo studies allowing for the sensitive detection of pathological changes in soft tissue and to provide quantitative three-dimensional data.It has been used in longitudinal studies to noninvasively monitor the genesis, progression and regression of a wide variety of diseases, reducing the need for interim sacrifice of animals at specified time points, thus allowing the same animal to be used as its own control within a given study. MRH is the use of MR imaging on formalin-fixed tissues for high resolution characterization of tissue structure. It is a highly valuable complimentary adjunct to conventional histopathology, as it permits a thorough examination to be performed through multiple digital slices of an entire organ, while leaving the formalin-fixed specimen intact for subsequent definitive conventional diagnostic histopathology.
Optimal fuzzy rule based pulmonary nodule detectionWookjin Choi
The document describes a lung cancer detection system that uses CT scans. It discusses (1) segmenting the lungs from CT images using adaptive thresholding and connected component analysis, (2) detecting nodule candidate regions using multi-thresholding and rule-based pruning, and (3) optimizing the rule-based pruning using a genetic algorithm trained fuzzy inference system to reduce false positives while maintaining high sensitivity. Experimental results on a publicly available lung image database show the optimized fuzzy system achieved better performance than a conventional rule-based approach.
PET scan uses radiotracers like FDG to produce 3D images showing biochemical functioning in the body. It has several components including detectors that detect gamma rays emitted by the radiotracer and a computer that analyzes the data to create images. PET scans are used to detect cancer, determine if cancer has spread, evaluate brain abnormalities, and more.
This document discusses various imaging techniques used in veterinary medicine, including fiberoptic endoscopy, computed tomography, magnetic resonance imaging, nuclear medicine techniques like scintigraphy, and fluoroscopy. It provides information on the principles, equipment, clinical applications, and procedures for each technique. Key points covered include how endoscopy can be used to evaluate the gastrointestinal tract, the physics behind computed tomography image formation using x-rays, the use of radioisotopes and radiopharmaceuticals in scintigraphy, and safety considerations for fluoroscopy due to radiation exposure.
This document discusses the basics of CT scanning, including its history and key components. It describes how CT scanning works, from the x-ray tube emitting radiation that is detected after passing through the body, to the computer using this data to reconstruct cross-sectional images. It outlines the main parts of a CT system, including the gantry, detector, and control console. It also explains different scanning methods and how image quality is determined.
Invasive Blood Pressure: fundamentals and best-practices for preclinical rese...InsideScientific
In this webinar sponsored by ADInstruments, Tom Smith, Professor of Orthopaedic Surgery at Wake Forest School of Medicine, and expert in microsurgery and vascular pressure measurement techniques discusses the fundamental properties of pressure measurements in the vascular system, including the history and physics behind solid-state manometry. In addition, he explains the importance and impact of high fidelity solid-state catheters as it relates to accuracy, consistency and research outcomes.
The SuperArgus state-of-the-art preclinical PET/CT system: An overview of the...Scintica Instrumentation
These systems are ideally suited for pre-clinical imaging of small animals such as mice and rats, all the way up to medium sized animals such as rabbits, non-human primates and other similarly sized animals. Some of the unique imaging capabilities include real-time imaging of awake animals, as well as multiplexed PET imaging of standard and non-standard isotopes. Key research applications and example images were reviewed.
Positron Emission Tomography (PET) is the gold standard in metabolic imaging, providing high sensitivity to specific radiotracer used to detect specific metabolic activity or biomarkers in vivo. The most common uses for PET imaging in pre-clinical research include oncology, neurobiology, cardiology, as well as dynamic imaging.
These systems are considered to be best in class imaging system with state of the art detectors and electronics. The systems have been designed to be self-shielded, requiring no additional shielding at the location selected for installation. The systems come in a three different bore sizes allowing for imaging of animals such as mice all the way up to rabbits and even non-human primates. The CT component of these systems has been optimized for reduced radiation exposure, rapid acquisition times, and high resolution images; all ideal for the longitudinal studies so commonly performed in pre-clinical research.
The SuperArgus system is uniquely designed to provide consistent resolution across the entire field of view, while maintaining sensitivity and system performance. Reconstruction algorithms have also been implemented to rapidly process and display the acquired images. The system performs very well for standard imaging applications such as oncology, cardiology, etc. Additionally, the system has some unique features which allow for some unique imaging capabilities such as real-time awake animal imaging, self-gated cardiac imaging, as well as multiplex imaging of standard and non-standard isotopes.
This document discusses the use of computers in veterinary surgery and medicine. It outlines the history of computers from Charles Babbage's concept in the 1830s to their use in veterinary science in the 1980s. Computers can be used as virtual labs to model drug effects, as simulators for surgical and medical training, and for data management in veterinary hospitals. They also assist with diagnosis, developing treatment plans, education, and various imaging and surgical techniques like digital radiography, ultrasound, CT scans, and MRI.
The document describes the IVM-MS intravital microscopy system from Scintica. The system provides an all-in-one solution for intravital imaging of various organs in live animals. It integrates hardware and software optimized for high-quality intravital imaging. The system includes components for animal maintenance during imaging and can perform functions like z-stack, mosaic, and time-lapse imaging. Examples shown include brain, tumor, lung, and heart imaging applications in window chamber models using confocal and two-photon microscopy.
Ultrasound Machine-A Revolution In Medical ImagingRAVI KANT
What is medical imaging?
Why ultrasound imaging is required?
History of ultrasound
What is ultrasound
Physical definition
Medical definition
Ultrasound production
The Returning echo
Doppler effect
What is Doppler ultrasound
Principles of instrumentation in ultrasonography
Transmitter and receiver circuits of ultrasound
Mechanical assembly of ultrasound machine
Manufacturing companies of USG
Sonoscape S40 color Doppler ultrasound system
Clinical applications of ultrasound
Future of ultraso
The document discusses problems caused by respiratory motion during radiotherapy treatment planning and delivery. It describes limitations in image acquisition, treatment planning, and radiation delivery due to organ motion. It then outlines several methods to account for respiratory motion, including motion encompassing techniques like slow CT, inhale/exhale breath-hold CT, and 4D CT. Respiratory gating techniques using external markers like the Varian Real-time Position Management (RPM) system or internal markers are also summarized. The RPM system and process for using external markers for respiratory gated imaging and treatment are described in detail.
Dr. Lawrence Yip explained how Photoacoustic (PA) imaging works, where it fits in with other modalities and, how your research could benefit from this emerging technology.
Excellent spatial resolution, depth penetration, and superior contrast are just some of the advantages often associated with PA imaging. In this webinar, we dove into the advantages, where they can be beneficial, and how the TriTom’s patented technology overcomes some of the challenges experienced by early adopters of this imaging modality.
The TriTom is a turnkey, compact, tabletop imaging system that combines the sensitivity of fluorescence molecular tomography with the depth penetration and spatial resolution of PA tomography. Many applications including cancer, neuroimaging, developmental biology, and cardiovascular research could benefit from adding these imaging modalities, and we will draw from literature and concrete examples to demonstrate this advantage.
Freedom: The Promise of Telemetry Revisited - Stellar Telemetry Webinar (TSE ...InsideScientific
The innovative Stellar Telemetry System allows individual recording of key physiological parameters in freely roaming and socially active animals. In this webinar we will discuss added capabilities of such a system, in particular flexibility and freedom for both scientist and subject. In one 'subtropical' setting we will learn how such a system can be used in a colony of primates that are free to roam, without traditional geographical or data management limitations. In the other setting we return to the lab and see how a system like this can enhance measurement capabilities in a traditional rodent model.
Lecture 3 & 4 anam sanam chick ldkfdlsfldfjdlsjfdlks .pptxfaiz3334
Computed tomography (CT) scans create cross-sectional images of the body by using X-rays and computer processing. An X-ray tube rotates around the body and produces multiple images from different angles, which are used to reconstruct cross-sectional slices using back projection. These slices can be combined to create 3D images. CT scans provide more detailed images than basic X-rays due to their ability to distinguish between different tissue densities and visualize structures throughout the body.
CT or CAT scanners use X-rays and computer technology to create cross-sectional images of the body. Godfrey Hounsfield invented the first commercially viable CT scanner in 1967. A CT scanner is composed of a gantry with an X-ray tube that rotates around the patient, detectors, and a data system. X-rays pass through the body and are measured by detectors. A computer uses reconstruction algorithms to generate images from the data. CT scans provide detailed images and have advantages like detecting multiple types of tissues, being fast and painless, and helping diagnose many conditions.
(March 13, 2024) Overview of Preclinical Small Animal and Multimodal ImagingScintica Instrumentation
In this webinar, we reviewed some of the most commonly used preclinical imaging modalities, including magnetic resonance imaging (MRI), positron emission tomography (PET), computer tomography (CT), ultrasound, photoacoustic, bioluminescence, fluorescence, dual-energy x-ray absorptiometry (DEXA/DXA), and intravital microscopy. For each modality, we spent time reviewing the basics of how each worked, the strengths and considerations of each, and some key application areas and example images. Finally, we discussed the benefits of multimodal imaging and reviewed a few papers utilizing a variety of imaging modalities to help support their research outcomes.
We ended with a very brief introduction to Scintica Instrumentation and our philosophy behind the various products we represented. However, the main focus of the webinar was on education, and not our diverse product portfolio.
Similar to Overview of Scintica’s Preclinical Imaging Product Portfolio: Technical Capabilities (20)
(June 12, 2024) Webinar: Development of PET theranostics targeting the molecu...Scintica Instrumentation
Targeting Hsp90 and its pathogen Orthologs with Tethered Inhibitors as a Diagnostic and Therapeutic Strategy for cancer and infectious diseases with Dr. Timothy Haystead.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
This presentation highlights the applications and capabilities of the M-Series™ compact MRI systems. Anatomical, functional, and molecular imaging can be performed on the M-Series and are often applied in cancer, cardiac, neuroscience, and multimodal imaging studies. It showcases example data from a variety of papers and training sessions in which the focus is on anatomy, neurobiology, and oncology. The presentation shows data from contrast agents which further enhances the capabilities of the M-Series, providing invaluable insights into tissue/tumor perfusion, myocardial infarction size, and molecular targets.
Ultrasound color Doppler imaging has been routinely used for the diagnosis of cardiovascular diseases, enabling real-time flow visualization through the Doppler effect. Yet, its inability to provide true flow velocity vectors due to its one-dimensional detection limits its efficacy. To overcome this limitation, various VFI schemes, including multi-angle beams, speckle tracking, and transverse oscillation, have been explored, with some already available commercially. However, many of these methods still rely on autocorrelation, which poses inherent issues such as underestimation, aliasing, and the need for large ensemble sizes. Conversely, speckle-tracking-based VFI enables lateral velocity estimation but suffers from significantly lower accuracy compared to axial velocity measurements.
To address these challenges, we have presented a speckle-tracking-based VFI approach utilizing multi-angle ultrafast plane wave imaging. Our approach involves estimating axial velocity components projected onto individual steered plane waves, which are then combined to derive the velocity vector. Additionally, we've introduced a VFI visualization technique with high spatial and temporal resolutions capable of tracking flow particle trajectories.
Simulation and flow phantom experiments demonstrate that the proposed VFI method outperforms both speckle-tracking-based VFI and autocorrelation VFI counterparts by at least a factor of three. Furthermore, in vivo measurements on carotid arteries using the Prodigy ultrasound scanner demonstrate the effectiveness of our approach compared to existing methods, providing a more robust imaging tool for hemodynamic studies.
Learning objectives:
- Understand fundamental limitations of color Doppler imaging.
- Understand principles behind advanced vector flow imaging techniques.
- Familiarize with the ultrasound speckle tracking technique and its implications in flow imaging.
- Explore experiments conducted using multi-angle plane wave ultrafast imaging, specifically utilizing the pulse-sequence mode on a 128-channel ultrasound research platform.
Accelerating the Delivery of New Treatments for Children with Neuroblastoma 2...Scintica Instrumentation
Neuroblastoma is a tumour arising from anomalies in the development of the sympathic nervous system and still accounts for 13% of all cancer-related death in children due to resistant, relapsing and metastatic diseases. There is an urgent need for the development of new treatment against high-risk relapsed neuroblastoma.
Overview:
Here we will discuss the ICR Paediatric Mouse Hospital approach which integrates more advanced mouse modelling, such as the use of genetically-engineered mouse (GEM) models and patient-derived xenografts to accelerate the discovery and evaluation of novel therapeutic strategies and help shape the clinical trial pipeline priorities for children with high-risk relapsing/refractory neuroblastoma.
We will also highlight the pivotal role of MRI within the Mouse Hospital which includes:
Enhancing and accelerating preclinical trials
Quantitatively inform on tumour phenotype and tumour response to treatment to:
Develop in vivo models that emulate the clinical treatment resistant phenotype using chemotherapy-dose escalation protocol
Characterize tumour spatial heterogeneity and evolution over treatment and guide the pathological and molecular characterization of the resistant phenotype
Finally we will also discuss how the compact, cryogen-free and user-friendly Aspect Imaging M-Series has transformed our way of working within the mouse hospital by providing a shared and easily accessible resource for tumour screening (with minimal onboarding) .
(March 14, 2024) Webinar: Validation of DEXA for Longitudinal Quantification ...Scintica Instrumentation
Noninvasive imaging is central to preclinical, in vivo models of pancreatic ductal adenocarcinoma (PDAC). While bioluminescent imaging (BLI) is a gold standard, its signal is dependent on the metabolic activity of tumor cells. In contrast, dual energy X-ray absorptiometry (DEXA) is a direct measure of body composition. Thus, this project aimed to assess the potential of using DEXA for longitudinal quantification of tumor burden versus BLI in an orthotopic KCKO murine model of PDAC. In short, DEXA successfully identified a growing tumor burden and accurately predicts ex vivo tumor mass in a time sensitive manner.
Learning objectives:
Learn to take advantage of DEXA for things other than bone density and bone health (i.e., lean, and fat mass)
Understand that DEXA can reproducibly and accurately be used to monitor tumor burden and growth in orthotopic murine models of pancreatic cancer
Understand the importance of repurposing techniques and equipment for new analysis
Understand that non-invasive in vivo imaging is crucially important in severely compromised models like those for PDAC and other cancers
See the value of utilizing multiple techniques throughout an experiment to enhance data collection
Overview:
In this webinar, Dr. Edwin C. Pratt discussed the realm of positron emission tomography (PET) imaging and explained the innovative concept of multiplexed PET. This new scientific advancement makes it possible to perform simultaneous imaging with two different isotopes providing more in depth information with a single scan.
Key Takeaways:
Multiplexed PET is a new reconstruction method to identify and separate positron from positron-prompt gamma emissions without new hardware from list mode PET scanners or energy discrimination of events.
Multiplexed PET is a quantitative method that is agnostic to the type of radiotracer used (IE no compartment modeling). Only a simple uniformity and sensitivity phantom is required.
Acquisition has been shown in a variety of preclinical and clinical PET scanners, though not all scanners can natively acquire data for multiplexing.
Multiplexed PET enables faster throughput for screening radiotracers, or conversely two tracer information of a tissue of interest, like imaging the tumor microenvironment for two immune populations.
(June 29, 2023) Webinar: Designer and Targeted Contrast Agent for Photoacoust...Scintica Instrumentation
Overview:
The talk focused on the synthesis, characterization and use of a novel contrast agent composed of indocyanine green dye for NIR-I photoacoustic (PA) imaging. The contrast agent can be easily tuned to different sizes without enclosure in nanocarriers, has strong optical absorption and PA signal at 895 nm, can be easily functionalized with different targeting molecules and can be imaged for 120 minutes in vivo. The presentation explained details on the genesis of the idea for building a biocompatible contrast agent and give details on its easy synthesis protocols, touch upon a functionalization scheme for adding targeting molecules and demonstrate its use as a PA contrast in mice using the TriTom small animal imaging system.
Photoacoustic imaging (PAI) is a noninvasive imaging modality that relies on absorption of laser light and thermal expansion of biological tissues, which generate ultrasonic waves. These ultrasound waves are then used to reconstitute an image of the tissues with anatomical details and functional information. To increase imaging depth and resolution, PAI requires exogenous molecular contrast agents with high optical absorption in the near infrared (NIR). However, the current repository of NIR dyes that are suitable for PAI is extremely limited. The FDA-approved indocyanine green (ICG) is the only commercially available contrast agent with NIR absorbance that is already used for PAI. However, ICG dyes suffer from poor photostability and high clearance rate.
In this webinar, Dr. Shrishti Singh presented a synthesis method for clinically translatable ICG-JA whose mean size can be finely tuned from 200 nm to 1000 nm and that does not require encapsulation in a nanocarrier. The talk will also detail complete characterization of the agent and steps for functionalization with targeting peptides or antibodies. Additionally, the webinar also provided details about the PA properties of the contrast in vitro in different conditions including whole blood, followed by details on the photoacoustic imaging in vivo using the TriTom system.
Learning Objectives:
Get details on the synthesis of a NIR contrast without the need of a nanocarrier.
Learn in detail about what characteristics a contrast agent should possess to qualify as a clinically translatable technology.
Become familiar with methods to create a targeted contrast agent.
This document compares two dual-energy X-ray absorptiometry (DXA) systems - PIXImus and Insight - for skeletal phenotyping in mice. It finds that while both systems can measure bone mineral density (BMD) and content (BMC) non-invasively and rapidly, they produce somewhat different quantitative results. PIXImus is a portable unit that uses low X-ray energies and high-resolution pixels, allowing measurements in low-density bone, while Insight requires a longer scanning time. Overall, the document evaluates the two DXA systems for analyzing skeletal changes in mouse models of bone disease.
(May 3, 2023) Webinar: Exploring a Novel NIR-2 Photoacoustic Agent to Improve...Scintica Instrumentation
The document introduces a novel biodegradable and biocompatible semiconductor nanocrystal called bornite that could improve photoacoustic imaging contrast for deep tissue applications. Experiments show bornite generates a 5x stronger photoacoustic signal than gold nanorods and indocyanine green. It also allows 2-3x deeper imaging of up to 5cm in tissue phantoms and provides around 2x better contrast in vivo. Bornite could be a safer and more effective photoacoustic contrast agent compared to existing alternatives.
(April 5, 2023) Webinar: Prodigy Open-Platform Research Ultrasound System Ov...Scintica Instrumentation
Overview:
In this webinar, we provided an overview of the Prodigy open-platform research ultrasound system. The Prodigy by S-Sharp is a flexible and powerful ultrasound platform enabling research in ultrasound imaging, high-intensity focused ultrasound (HIFU), non-destructive testing (NDT), and much more. Sold for many years as an OEM component of other systems (e.g., for photoacoustic imaging), this highly capable system is now available to laboratories and researchers around the world.
This compact, high-performance ultrasound system is optimized for a variety of engineering research applications. As an open platform research ultrasound system, the Prodigy allows almost every aspect of ultrasound generation and detection to be customized. This includes true arbitrary transmit waveforms, super-fast acquisition capabilities, rapid data transfer, and a software backend that allows for real-time access and processing of both raw and beamformed data.
Some highlights of the Prodigy include its capability for true arbitrary transmit waveforms by using linear amplifiers with digital-to-analog converters (DAC) and the availability of a graphic user interface for designing pulse sequences and adjusting transmit/receive parameters.
Learn the capabilities of this flexible system with peer-reviewed examples of its many possible applications.
Key Points:
(April 4, 2023) Overview of Preclinical Small Animal Imaging Modalities & Mul...Scintica Instrumentation
Overview:
In this webinar, we will review some of the most used preclinical imaging modalities, including magnetic resonance imaging (MRI), positron emission tomography (PET), computer tomography (CT), ultrasound, photoacoustic, bioluminescence, fluorescence, dual-energy x-ray absorptiometry (DEXA/DXA) and intravital microscopy. For each modality, we will spend time reviewing the basics of how each works, the strengths and considerations of each, and some key application areas and example images. Finally, we will discuss the benefits of multimodal imaging and review a few papers utilizing a variety of imaging modalities to help support their outcomes. This webinar will introduce our educational focus on preclinical imaging modalities coming up in 2023.
The webinar will be a brief introduction for those who need to become more familiar with all or some of the preclinical imaging modalities. At the same time, our educational focus over the year will dive deeper into each modality, talk more in-depth about multimodal imaging and its benefits, and explore some of the newer topics emerging in the preclinical imaging world, including theranostics, contrast agent development, and many others. Please join us as we start this journey and continue to check back as we expand upon the basics introduced during this webinar.
Learning Objectives:
Understand convection-enhanced delivery and its implication for brain tumour treatment
Understand how gold nanoparticles can be used to construct radiation nanomedicine
Learn how to evaluate the safety, toxicity, and effectiveness of radiation nanomedicines
Overview:
Glioblastoma is a devastatingly aggressive type of brain tumour with a low median, and 5-year survival that has lacked new treatment options, in part due to the inability of therapeutic agents to cross the blood-brain barrier. Convection Enhanced Delivery (CED), a clinical neurosurgical strategy has been used to locoregionally deliver various therapeutic agents within the brain. Radiotherapeutic agents, such as 177Lu-labeled gold nanoparticles (177Lu-AuNP), hold promise for treatment of glioblastoma when administered by CED. Intratumoural injections of 177Lu-AuNP administered by CED was evaluated in an orthotopic xenograft mouse model of glioblastoma. SPECT/CT and biodistribution studies were used to evaluate the fate of the 177Lu-AuNP after injection. These results were used to estimate organ radiation absorbed doses. Normal tissue toxicity was evaluated to confirm the safety of the injections. Magnetic resonance imaging and bioluminescence imaging were used to monitor tumour growth after administration of 177Lu-AuNP, and median survival was estimated.
(February 16, 2023) Webinar: Intracerebral Transplantation of Autologous Bone...Scintica Instrumentation
Overview:
In this webinar, Max Myers presented his work on the use of autologous bone marrow-derived stem cells injected into the cortex of rats, following a stable stroke. Max also demonstrated its lab’s findings and talked about the Aspect Imaging M7 compact MRI system as it relates to its use in this project.
Key Points:
The critical use of stem cells in stroke research
Overcoming the blood-brain barrier via intracerebral injection of stem cells
The introduction of stem cells led to improved functional recovery following an ischemic stroke
How MRI can contribute to the understanding of treatments following stroke
Sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) uncoupling in skeletal muscle and mitochondrial uncoupling via uncoupling protein 1 (UCP1) in brown/beige adipose tissue are two primary mechanisms implicated in energy expenditure. Here, the effects of glycogen synthase kinase 3 (GSK3) inhibition via lithium chloride (LiCl) treatment on SERCA uncoupling in skeletal muscle and UCP1 expression in adipose were investigated. C2C12 and 3T3-L1 cells treated with LiCl had increased SERCA uncoupling and UCP1 protein levels, respectively, ultimately raising cellular respiration; however, this was only observed when LiCl treatment occurred throughout differentiation. In vivo, LiCl treatment (10 mg/kg/day) increased food intake in chow-fed and high-fat diet (HFD, 60% kcal) fed male mice without increasing body mass – a result attributed to elevated daily energy expenditure.
In soleus muscle, the lab determined LiCl treatment promoted SERCA uncoupling via increased expression of SERCA uncouplers, sarcolipin and/or neuronatin, under chow and HFD-fed conditions. They attribute these effects to the GSK3 inhibition observed with LiCl treatment as partial muscle specific GSK3 knockdown produced similar effects. In adipose, LiCl treatment inhibited GSK3 in inguinal WAT (iWAT) but not in brown adipose tissue under chow-fed conditions, which in turn led to an increase in UCP1 in iWAT and a beiging-like effect with a multilocular phenotype. The beiging-like effect was not observed, and increase in UCP1 when mice were fed a HFD, as LiCl could not overcome the ensuing overactivation of GSK3. Nonetheless, the study establishes novel regulatory links between GSK3 and SERCA uncoupling in muscle and GSK3 and UCP1 and beiging in iWAT.
This document summarizes research on molecular mechanisms behind lameness in meat chickens. The research found alterations to bone homeostasis and bacterial immune responses that contribute to lameness. Specifically, it was found that bacterial infection dysregulates genes involved in mitochondrial function, dynamics, and biogenesis in bone cells, leading to mitochondrial dysfunction, increased cell death, and disruption of cellular processes. Additionally, genes related to the autophagy pathway were downregulated in lame chickens, suggesting bacterial infection impairs autophagy in bone tissue. The research provides insights into how bacteria may cause lameness at the molecular level by interfering with mitochondrial health and autophagy in leg bones.
In this webinar, Katie will discuss the role hypoxia plays in disease progression and treatment response, specifically in cancer. She will also dive into the various molecular imaging technologies that can be used to visualize and assess hypoxia in preclinical cancer models. Some modalities that will be covered include magnetic resonance imaging (MRI), positron emission tomography (PET), and optical imaging.
Topics to be covered:
What is hypoxia?
Is there a link between hypoxia and cancer?
What imaging modalities can be used to visualize hypoxia in vivo?
What are the advantages and limitations of each technique?
What are some applications of hypoxia imaging?
Hypoxia has been shown to influence many facets of cancer including tumor growth, treatment response, and metastatic potential. Thus, the ability to noninvasively visualize hypoxia in vivo may be critical to understanding the underlying tumor biology, guiding treatment plans, and determining prognosis in the clinic.
Many different modalities have been used for preclinical hypoxia imaging. While some techniques have been around for decades and have extensive data behind them, others are emerging technologies that aim to overcome existing limitations in the field. Choosing the right modality can be challenging and is dependent on experimental conditions including tumor model, animal strain, and the desired measurement, as each technique will target a different aspect of hypoxia. In this webinar, we will discuss some molecular imaging techniques that can be used to visualize and characterize tumor hypoxia including MRI, PET, optical, and PAI. We will compare the various options, discuss the advantages and limitations of each approach, and show some examples of how scientists are using these techniques within their research.
References
Rebecca A. D’Alonzo, Suki Gill, Pejman Rowshanfarzad, Synat Keam, Kelly M. MacKinnon, Alistair M. Cook & Martin A. Ebert (2021) In vivo noninvasive preclinical tumor hypoxia imaging methods: a review, International Journal of Radiation Biology, 97:5, 593-631, DOI: 10.1080/09553002.2021.1900943
(December 2, 2021) The Bench to Bedside Series: Preclinical Cancer Research w...Scintica Instrumentation
Overview:
The goal of this webinar will be to provide a high-level overview of the various stages of preclinical cancer research and discuss the role that innovative instrumentation can play in moving science forward.
To better understand how to treat and control cancer, researchers start by investigating the basics – the cells, molecules, and genes that make up the human body. This type of study, which is often referred to as basic or discovery research, aims to understand the underlying mechanisms contributing to cancer growth and spread. This knowledge is an essential starting point for developing future diagnostic tests and treatment strategies.
After finding an innovative idea that works in cells, researchers need to take their studies to the next level by employing animal models that have similar biology to humans. Animal models have helped scientists make some of the most important cancer discoveries over the years. Furthermore, preclinical imaging technologies allow researchers to perform longitudinal animal studies that are noninvasive leaving the underlying biology intact so that one can track changes throughout the entire disease process.
It was previously thought that the journey from bench to bedside was unidirectional, starting with discovery research and moving towards clinical trials. However, in the last decade, it has become crucial for basic scientists and clinicians to work together towards finding innovative solutions that will positively impact patient care.
After attending this webinar, we hope you will have a better understanding of the preclinical workflow needed to push an idea from bench to bedside as well as some of the key equipment that is needed along the way.
This webinar series will be hosted by Drs. Katie Parkins and Tyler Lalonde, both of which have extensive experience in translational research areas including oncology, neuroscience, molecular imaging, and drug development.
In this webinar we will discuss the following topics:
• Introduction To Cancer Research
• What does “Bench to Bedside” mean?
• In vitro characterization
• Rapid throughput screening
• Quantitative tools
• Moving towards translation
Overview:
Muscles are vital for everyday life, from every move we make to every beat of the heart. Conditions that lead to muscle wasting can drastically reduce our health and quality of life. This presentation will discuss the possibility of inhibiting an enzyme called glycogen synthase kinase 3 (GSK3) for the treatment/management of muscular dystrophy and spaceflight.
Without providing too much detail we will show our results with tideglusib treatment - a clinically advanced GSK3 inhibitor - on mdx mice. We will also discuss some of our ideas moving forward with spaceflight and how we plan on leveraging new infrastructure.
Objectives:
The importance of muscle health for overall health
Glycogen synthase kinase 3 and its role in regulating muscle size and composition
Calcium regulation in the heart
Muscular dystrophy
Spaceflight
(October 12, 2021) Webinar: Clinical Field MRI As A Measurement Instrument fo...Scintica Instrumentation
Watch our webinar where Professor Marc-Andre Fortin presented about the 3D printing of hydrogels and hydrated substances that have been introduced in various fields of biomedical research including regenerative medicine, cosmetic surgery, orthopedics, and medical physics.
However, one of the main challenges faced by 3D printing and bioprinting is geometrical conformity. In this presentation, studies requiring hydrogel 3D printing in the fields of ophthalmology, regenerative medicine, and medical physics, were described. MRI scanning procedures were developed and optimized for these specific applications.
The presentation highlighted the potential role of MRI in the development of more accurate, more precise 3D-printed hydrogel objects.
CapTechTalks Webinar Slides June 2024 Donovan Wright.pptxCapitolTechU
Slides from a Capitol Technology University webinar held June 20, 2024. The webinar featured Dr. Donovan Wright, presenting on the Department of Defense Digital Transformation.
Brand Guideline of Bashundhara A4 Paper - 2024khabri85
It outlines the basic identity elements such as symbol, logotype, colors, and typefaces. It provides examples of applying the identity to materials like letterhead, business cards, reports, folders, and websites.
THE SACRIFICE HOW PRO-PALESTINE PROTESTS STUDENTS ARE SACRIFICING TO CHANGE T...indexPub
The recent surge in pro-Palestine student activism has prompted significant responses from universities, ranging from negotiations and divestment commitments to increased transparency about investments in companies supporting the war on Gaza. This activism has led to the cessation of student encampments but also highlighted the substantial sacrifices made by students, including academic disruptions and personal risks. The primary drivers of these protests are poor university administration, lack of transparency, and inadequate communication between officials and students. This study examines the profound emotional, psychological, and professional impacts on students engaged in pro-Palestine protests, focusing on Generation Z's (Gen-Z) activism dynamics. This paper explores the significant sacrifices made by these students and even the professors supporting the pro-Palestine movement, with a focus on recent global movements. Through an in-depth analysis of printed and electronic media, the study examines the impacts of these sacrifices on the academic and personal lives of those involved. The paper highlights examples from various universities, demonstrating student activism's long-term and short-term effects, including disciplinary actions, social backlash, and career implications. The researchers also explore the broader implications of student sacrifices. The findings reveal that these sacrifices are driven by a profound commitment to justice and human rights, and are influenced by the increasing availability of information, peer interactions, and personal convictions. The study also discusses the broader implications of this activism, comparing it to historical precedents and assessing its potential to influence policy and public opinion. The emotional and psychological toll on student activists is significant, but their sense of purpose and community support mitigates some of these challenges. However, the researchers call for acknowledging the broader Impact of these sacrifices on the future global movement of FreePalestine.
6. • Prospect T1 System Technical Overview
• Sample images acquired during the live virtual demo (Oct 1)
Topics of Discussion
7. Prospect T1 System Technical Overview
• System components and standard configuration
• Add-on hardware and software components
8. Prospect T1
System Components
• The Prospect T1 is the first tablet based high-frequency
ultrasound system specifically designed for pre-clinical
imaging of small animals
• System components:
• Tablet
• Probe
• Scanning Platform
9. Prospect T1
System Components:
Probes
• Three single element probes are available:
• 20 MHz (user selectable between 15-30 MHz)
• Primarily used for rat imaging, as well as harmonic contrast imaging
• 40 MHz (user selectable between 30-50 MHz)
• Primarily used for mouse imaging, and superficial anatomical
targets in larger species like rats
• 50 MHz (user selectable between 30-50 MHz)
• Primarily used for superficial anatomical targets in both mice and
rats
10. Prospect T1
System Components:
Scanning Platforms
• The platform is compact in design, again to limit the footprint of
the system
• The scanning platform has been designed for ergonomical
positioning of the probe
• Animal beds have integrated heating, and ECG and
respiratory monitoring
• Interchangeable beds are available for mice or rats
• Animal beds can be precisely adjusted in the X, Y, and Z axis
11. Standard System Configuration
• Standard system configuration for mouse
• B-Mode
• M-Mode
• Pulsed Wave / Color / Power / Tissue Doppler Mode
• Contrast Mode
• Comprehensive Measurement and Analysis Tools
• Scanning Platform – with mouse bed
• 40 MHz probe
• Standard system configuration for rat
• B-Mode
• M-Mode
• Pulsed Wave / Color / Power / Tissue Doppler Mode
• Contrast Mode
• Comprehensive Measurement and Analysis Tools
• Scanning Platform – with rat bed
• 20 MHz probe
12. Add-Ons:
3D Motor
• The 3D motor expands the capabilities of the Prospect T1 to
acquire 3D B-mode images
• Add-on includes the software analysis package to view the 3D
images and perform volume calculations
13. Add-Ons:
Image Guided Needle
Injection Mount
• The image guided needle injection mount integrates with probe
• Injections may be performed with a regular syringe and steel
needle, or pulled glass capillary needle
• Injections may be made into developing embryos, adult
myocardium, or abdominal/muscle targets
E15.5 mouse
embryo
Adult mouse
myocardium
14. Sample Images
• Images acquired during the live virtual demo on Oct 1
• Additional images to show capability not shown in the live demo
15. Tumour Imaging – 2D B-Mode Imaging
• Tumour identification, and basic linear and area measurements
• Investigation of surrounding structures
• Longitudinal imaging to monitor tumor progression or
therapeutic response
Orthotopic Mammary Fat Pad
Tumour MDA-MB-231
• Power Doppler (not shown during the demo due to a technical issue with the demo system)
can be used to assess tumor vasculature using endogenous signal within the tissue
• Contrast Imaging (linear and subharmonic - not shown during the demo as no contrast
agent was available) can be used to assess tumor microvasculature using microbubbles
16. Tumour Imaging – 2D B-Mode Imaging
• The complex nature of tumors can be investigated using B-Mode imaging, as well as surrounding structures
IP Injection of Ovarian Tumour Cells
SKOV-3
Tumour
Stomach
IP Injection of Ovarian Tumour Cells
SKOV-3
Kidney Splenic Vein
TumourIntestine
17. Tumour Imaging – 3D B-Mode Imaging
• 3D volume measurements
• Longitudinal imaging to monitor
tumor progression or therapeutic
response
Volume = 263mm3
Orthotopic Mammary Fat Pad
Tumour MDA-MB-231
18. Tumour Imaging – 3D B-Mode Imaging
• 3D volume measurements
• Complex tumor structures
can be visualized
• Longitudinal imaging to
monitor tumor progression or
therapeutic response
IP Injection of Ovarian Tumour Cells
SKOV-3
Tumo
ur
22. Cardiovascular Imaging – Left Carotid Artery
• Color Doppler (not shown during the demo due to a technical issue with the demo system)
can be used to identify and assess blood flow velocity and direction within the heart and
surrounding vasculature
24. Topics of Discussion
• M-Series System Technical Overview
• Sample images acquired during the live virtual demo (Oct 1)
25. M-SeriesTM System Technical Overview
• M-SeriesTM Compact magnet design
• Animal handling system
• SimPET insert option for simultaneous PET/MR imaging
26. System Components
• Compact magnet – select either the M3, M5, or M7 magnet;
• Electronics cabinet and User Workstation - is the same for all
systems.
• Accessories and add-ons
• Animal handling system with heating and physiological monitoring
• Anesthesia delivery and exhaust gas scavenging
• SimPETTM insert – simultaneous PET/MRI (M7 system only)
27. M-SeriesTM Compact MRI Systems from Aspect Imaging
M3 – Mouse Only M7 – Mouse & Large RatsM5 – Mouse & Small Rats
28. M-SeriesTM Compact Magnet Design
• Require no special infrastructure
• Compact and self shielded with minimal external fringe field
• Operate very quietly during image acquisition
• Systems are installed with first images being acquired in less
than 1 day
Installed within an animal facility, or existing laboratory
next to other equipment or furnishings
29. Animal Handling System
• Fully integrated animal handling and coil system includes
• Mouse or rat beds to suite varying animal sizes
• Anatomy specific coils, with automatic tuning
• Heating
• Physiological monitoring
• Anesthesia delivery and scavenging
30. Imaging Coils
Type
Dimensions
Application
Inner Diameter Length
Mouse Head 23 mm 25 mm Neurological imaging in mice
Mouse Body 30 mm 50 mm
Extremity, abdominal, and thoracic cavity imaging in
mice
Mouse Whole Body 30 mm 80 mm Whole body imaging in mice
Large Mouse Body 38 mm 50 mm
Extremity, abdominal and thoracic cavity imaging in
large/obese mice
Rat Head 35 mm 40 mm Neurological imaging in rats
Rat Body 50/60 mm ellipsoid 90 mm
Extremity, abdominal and thoracic cavity imaging in
rats
Large Rat Body 71 mm 90 mm
Extremity, abdominal and thoracic cavity imaging in
large rats
Imaging coils should fit as tightly as
possible to the anatomical target for high
quality images
32. SimPET insert option for simultaneous PET/MR imaging
• The SimPETTM insert expands the capabilities of the M7 system to allow
for simultaneous PET/MR imaging
• MR images compliment the highly sensitive PET images in detecting
functional information, abnormalities, and early disease, providing an
anatomical context
34. Tumour Imaging – Orthotopic Mammary Fat Pad Tumour
• MDA-MB-231 cell line
• T1 and T2 weighted image contrast helps
to identify anatomy; along with
pathological changes, including tumors
• 250µm in-plane resolution with 1mm slice
thickness
• Acquisition was around 4.5 minutes
T1 Weighted T2 Weighted
35. Tumour Imaging – Orthotopic Mammary Fat Pad Tumour
• Tumour volume was found to be 273mm3
T1 Weighted T2 Weighted
MDA-MB-231
36. Tumour Imaging – IP Injected Ovarian Tumour Cells
• SKOV3 cell line, injected IP
• These tumors may form anywhere within the
peritoneal cavity
• Numerous tumors were located throughout the
abdomen
T2 WeightedT1 WeightedT1 Weighted T2 Weighted
37. Tumour Imaging – IP Injected Ovarian Tumour Cells
• VivoQuant was used to visualize the acquired images in 3D and
to quantify the tumor volumes.
• Various manual and semi-automatic tools are available
T2 WeightedT1 Weighted
Region Of
Interest
Color
Volume
(mm³)
Upper Tumour red 144
Mid Tumour green 6
Lower Tumour blue 9
Lower Tumour #2 cyan 7
Mid Tumour #2 magenta 64
38. Brain Imaging – Normal Brain
T2 WeightedT1 Weighted
• Various structures can be visualized within the brain
• 200µm in-plane resolution, 1mm slice thickness; 7.5-minute acquisition time
40. Newton 7.0 FT
In Vivo Optical Imaging System
COURTESY : Matthieu Germain , Nanobiotix/Curadigm
41. TOPICS OF DISCUSSION
• Applications of optical imaging
• Product overview
• Images acquired during the live demo
42. Main applications of optical imaging
Luciferase
expressing
metastases
mCherry
expressing
cancer cells
Fluorescence Imaging Bioluminescence Imaging
• Oncology
• Neurology
• Biodistribution studies
• Treatment studies (BLI)
• Cell tracking
• Ex vivo imaging
43. Proprietary CCD Camera
16-bit Scientific CCD
Camera
Grade 0 – No dead pixels
NIST calibrated
4.8 Orders of Magnitude
Key Technical Features of the Newton 7.0 FT
CAMERA
DARQ-9
44. Key Technical Features of the Newton 7.0 FT
CAMERA
RESOLUTION
4.6 MP
Newton 7.0 FT / 4.6MP Other systems / >1MP
Better Performance in patter recognition
45. Key Technical Features of the Newton 7.0 FT
LENS APERTURE
F/0.70
Newton 7.0 FT = f/0.70 Other systems = f/0.95
More light collection = More sensitivity
Wider lens
aperture
Smaller lens
aperture
46. Key Technical Features of the Newton 7.0 FT
MOTORIZED
DARKROOM
Infinite imaging Capabilities
47. Key Technical Features of the Newton 7.0 FT
Green
520nm
Green
580nm
Red
640nm
IR
740nm
Blue
480nm
NIR
680nm
IR
780nm
Blue
420nm
Full Spectrum Tunability
• 8 Excitation channels (400nm > 800nm)
• Powerful Laser Class II illumination
• Reducing the cross stimulation
• Increasing the sensitivity of your
images
48. Key Technical Features of the Newton 7.0 FT
NARROW BAND-PASS FILTERS
Emission Filters
• 8 Narrow Bandpass emission filters (500nm >
900nm)
• Dual Magnetron sputter-coated technology
• Ensuring transmission above 90%
• Improving spectral separation for multi-spectral
imaging
49. Key Technical Features of the Newton 7.0 FT
CCD camera
3D
NIR
Camer
a
3D
NIR
Camer
a
How 3D Optical Tomography is achieved in the Newton
7.0 ?
• First, the Topographic image of the mouse is acquired with
the help of 3D NIR Cameras
• Then, the main CCD camera acquires multiple images of
the signal using various emission filters from wavelengths
in the spectral emission of the specific probe used.
• The signal is reconstructed with the use of an algorithm
and repositioned within the topographic image of the
animal.
50. Key Technical Features of the Newton 7.0 FT
Co-registration with digital Organ & Bones
Atlas
• Choose to add or hide individual organs and
bones
• Axial, Sagittal and Coronal Views
• Quantification of the signal in units of volume
55. TOPICS OF DISCUSSION
• Product Overview
• What Makes the Sedecal Systems Unique
• Multi-animal Handling System
56. • Self-shielded to meet FDA guidelines
• no special room preparation, controls, or
additional shielding required
• Systems can easily be placed within existing
laboratory environments, imaging cores, or
within the animal facility
• Can have the following integrated into animal
handling:
• Anesthesia – isoflurane with gas scavenging
• Heating
• Physiological monitoring
• Image may be saved as DICOM, Interfile, or JPEG
REVIEW OF SUPERARGUS AND COMPACT MODELS
57. REVIEW OF SUPERARGUS AND COMPACT MODELS
• Compact (Mouse only) Model
• PET or CT
• Dimensions : 60 x 60 x 40cm
• 55 mm bore
• Static FOV: 45mm axial; 100mm
trans-axial
• CT Specifications
• 65µm resolution (max.)
• 15sec acquisition time (min.)
• PET Specifications
• 32 tDOI Phoswitch detectors; 4 rings
• ≤1.0mm resolution using 3D OSEM
reconstruction (≤2 min to perform)
58. • Super Argus Models (PET and/or CT)
• r models – mouse, rat, marmoset
• 90mm bore
• FOV – 220mm axial (dynamic); 80mm transaxial
• R models – up to 3kg rabbit
• 160mm bore
• FOV – 350mm axial (dynamic); 120mm transaxial
• P models – up to non-human primate, canine, porcine, etc.
• 260mm bore
• FOV – 650mm axial (dynamic); 210mm transaxial
• 2, 4, or 6 PET ring options for a 50mm, 100mm, or 150mm fixed axial FOV
• CT is focused on low does, high resolution (15µm) rapid scanning options (15sec)
REVIEW OF SUPERARGUS AND COMPACT MODELS
59. • Core Phoswich detector technology along with the electronics provide the following best in
class specifications:
• Highest resolution on the market (≤1.0mm)
• Resolution uniformity across the entire FOV, with FOV filling majority of the bore size due
to the tDOI technology correcting the parallax error
• Highest sensitivity (11%; at 100-700keV)
• Real-time imaging (up to 2.5msec frame rate if desired)
• Dynamic imaging – possible using time stamp acquisition electronics
• Parallel electronics acquisition
SUPERARGUS IS CONSIDERED TO BE BEST IN CLASS
60. MULTI-ANIMAL HANDLING SYSTEM
• Up to 4 mice can be scanned simultaneously in the R model
(160mm bore) using the multi-animal handling system
• Individually controlled anesthesia flow and heating controls
• Separate animal preparation workstation is also included
• Additional configurations are available for rats and/or mice
depending on specific needs
61. MULTI-ANIMAL HANDLING SYSTEM
Resolution and
sensitivity are
maintained throughout
the entire FOV due to
the unique tDOI
Phoswich detectors –
correcting for the
parallax error
62. • Sample images acquired during the live virtual demo (Oct 1)
Understanding the Complementar y Nature of Imaging Modalities
63. Understanding the Complementar y Nature of Imaging Modalities
T1 Weighted
• Bioluminescence helps to identify where the tumors may be located, however differentiating tumors from one another, and
measuring tumor volume is better done using an anatomical imaging modality such as MRI or Ultrasound
Kidney Splenic Vein
TumourIntestine
IP Injection of SKOV3 Ovarian
Tumour Cells
64. Understanding the Complementar y Nature of Imaging Modalities
T2 Weighted
• Bioluminescence helps to confirm viability of the tumor cells, as they express luciferase, approximate volumes may be
possible from the BLI signal; anatomical images help to confirm tumor volume - ultrasound (263mm3) or MRI (273mm3)
Orthotopic Mammary Fat Pad
Tumour (MDA-MB-231)
66. Katie Parkins, PhD
Preclinical Imaging Specialist
kparkins@scintica.com
Tonya Coulthard, MSc
Manager, Imaging Division
Tcoulthard@scintica.com
Overview of Scintica’s
Preclinical Imaging Product
Portfolio:
Technical Capabilities
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