DNA cloning is the process of making multiple, identical copies of a particular piece of DNA. In a typical DNA cloning procedure, the gene or other DNA fragment of interest (perhaps a gene for a medically important human protein) is first inserted into a circular piece of DNA called a plasmid.- [https://www.khanacademy.org/science/...dna.../dna-cloning.../a/overview-dna-cloning]
these slides have general information about somoclonal and gametoclonal variation... and have flow chart explain how this will be done and have many notefication related to the mutagenic agent and the genetic disorder and the detection.
In humans, approximately 25,000 genes exit among the 3 billion base pairs of DNA in the genome.
To study anyone of these genes, a researcher first isolates it from all of the other genes in an organisms DNA.
One isolation method has a relatively long history and involves the construction of a DNA library
When a gene is identified and copied, it is said to have been “cloned”
Recombinant Antibody Overview I - Creative BiolabsCreative-Biolabs
Hello. The slide is produced by Creative Biolabs who has extensive experience in therapeutic antibody and recombinant antibody production. In this video, we will give you a whole understanding to the conventional antibody, recombinant antibody and their basic information and expression.
An overview on role of signal transduction in inducing plant innate immunity which includes both systemic acquired resistance as well as induced systemic resistance.
DNA cloning is the process of making multiple, identical copies of a particular piece of DNA. In a typical DNA cloning procedure, the gene or other DNA fragment of interest (perhaps a gene for a medically important human protein) is first inserted into a circular piece of DNA called a plasmid.- [https://www.khanacademy.org/science/...dna.../dna-cloning.../a/overview-dna-cloning]
these slides have general information about somoclonal and gametoclonal variation... and have flow chart explain how this will be done and have many notefication related to the mutagenic agent and the genetic disorder and the detection.
In humans, approximately 25,000 genes exit among the 3 billion base pairs of DNA in the genome.
To study anyone of these genes, a researcher first isolates it from all of the other genes in an organisms DNA.
One isolation method has a relatively long history and involves the construction of a DNA library
When a gene is identified and copied, it is said to have been “cloned”
Recombinant Antibody Overview I - Creative BiolabsCreative-Biolabs
Hello. The slide is produced by Creative Biolabs who has extensive experience in therapeutic antibody and recombinant antibody production. In this video, we will give you a whole understanding to the conventional antibody, recombinant antibody and their basic information and expression.
An overview on role of signal transduction in inducing plant innate immunity which includes both systemic acquired resistance as well as induced systemic resistance.
Presentation at the March 2013 dialogue workshop of the Biosciences for Farming in Africa media fellowship programme in Accra, Ghana.
Please see www.sti4d.com/b4fa for more information
What Remains to be Discovered: Unlocking the Potential of Modern BiosciencesSIANI
Presented at the workshop "Moving Africa Towards a Knowledge based Bio-economy: How can Sweden assist?" organised by the SIANI Bio-economy Expert Group. More at: http://www.siani.se/news/siani-bioeconomy-expert-group-business
A transplastomic plant is a genetically modified plant in which the new genes have not been inserted in the nuclear DNA but in the DNA of the chloroplasts.
Efficient, quick and tissue culture independent system for crop plants improvement useful for those plants that lack tissue culture and regeneration system.
Two most common Agrobacterium mediated in-planta methods such as floral dip and vacuum infiltration have been successfully used by many researchers in both dicot and monocot plants.
Main advantages of in-planta transformation are to produce large number of transgenic plants and accumulation of high concentration of total soluble protein in short time.
Establishment of an in vitro propagation and transformation system of Balani...PGS
This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University
Abstract
Balanites aegyptiaca is a drought-tolerant but salt-sensitive tree species distributed in the tropical and arid lands in Africa and Asia; the seeds were used in biodiesel production. This study aimed to establish an in vitro propagation system of two B. aegyptiaca provenances from nodal and cotyledon explants. The explants were placed on Murashige and Skoog medium supplemented with different concentrations of 6-benzyladenine (BA) and thidiazuron (TDZ) for shoot induction. BA was significantly more effective in shoot induction from nodal explants. Three different Agrobacterium tumefaciens strains (EHA105, GV3101, and LBA4404) harboring the plasmid pCAMBIA2301 containing the nptII marker and gus reporter genes were used to establish a transformation system in B. aegyptiaca. Strain GV3101 resulted in the highest survival rates and highest number of explants positive in the GUS assay. This selected A. tumefaciens strain was used to introduce pBinAR containing the sequence encoding ERD10 (early responsive to dehydration 10) to produce salt-tolerant B. aegyptiaca plants.
Dendrobium
Family – Orchidaceae
Exhibits a vast diversity in vegetative and floral characteristics
1,600 Dendrobium species are recognized worldwide
High value of crop – for flower and medicinal purpose
D. husohanense- Anti-tumor and Anti-
inflammatry
D. longicornu - used to treat fever and
coughs
Micropropagation
Seeds are minute and lack endosperm
Micropropagation has been achieved using
Shoot tip culture
Seed culture (Immature and mature embryo)
Auxiliary Bud culture
Pseudobulb segment culture
Shoot Tip culture
Sterlization of Explant
Shoot tips(0.5–0.8 cm) harvested from mother plants
carefully washed in distilled water
surface decontaminated with 0.1% streptomycin (20 s) 70% (v/v) ethanol
(50 s) and 0.1% (w/v) HgCl2 (2 min)
Thoroughly rinsed with sterilized distilled water
Media
Subculturing
Micropropagation of Dendrobium From Pseudobulb segment
Regeneration from Pseudobulb Segment Cultures
Pseudobulb segments of about 0.5-1.0 cm excised from the 1 year old in vitro raised seedlings
Any leaves or roots, if present, were removed from the segments prior to inoculation
Each segment had one or two axillary buds. Single pseudobulb segment was cultured in test tubes (25 mm × 150 mm), each containing 12 mL half-strength MS basal medium supplemented with BAP 1.0 mg L-1 individually or in combination with NAA at 1 mg L-1
The medium was solidified with 4 g L-1 agar
Rooting of Regenerated Shoots (Pseudobulbs)
Small clumps of shoots having 2-3 pseudobulbs (3-4 cm in length) were cultured in test tubes (25 mm × 150 mm)
Each containing 12 mL half-strength MS basal medium supplemented with or without 1.0 mg L-1 IAA or IBA or NAA.
The cultures were incubated for 3 months under the conditions as described above.
The pH was adjusted to 5.8 before autoclaving at 121°C, 15 lb in-2 for 15 min.
Micropropagation of Dendrobium From Auxiliary bud
Stem (1–2 cm long), each comprising a node and axillary bud are used as explant- wash in running tape water for 15-20 minute
Surface sterilization with-
- 10 % (v/v) NaClO solution for 10 minute
- 0.1 % (w/v) HgCl2 for 2 min
- washing 5–6 times with sterilized distilled water
The explants were shortened to 3–4 mm after the removal of leaves, dry sheaths and other external tissues
Micropropagation of Dendrobium from immature seeds
Capsules collected from hand-pollinated plants after 8–14 wk of pollination
Surface-disinfected in 70% ethanol for 30 s, followed by 1.0% sodium
hypochlorite with two drops of Tween 20 per 100 ml for 10 min and rinsed five
times with sterile distilled water
After sterilization, the c
The presentation describes the advantages of plastid transformation over 'conventional' nuclear transformation, hurdles to plastid transformation, its advantages. The presentation also covers some successful plastid engineering and its potential.