The document describes a new Bacillus consortium product called Novo MAC (Novozymes Biologicals, Inc.) that contains the BioSTM microbial blend. Testing showed the BioSTM blend exhibited higher enzyme activity and a broader range of degradation compared to the market-leading MSB blend. It also produced superior levels of cellulase, lipase, and protease enzymes, resulting in faster breakdown of proteins, fats, oils, grease, and starches. This makes BioSTM more effective at eliminating buildups and odors in drains, grease traps, and septic systems. Studies showed BioSTM to be safe for use.

1. NOVOZYMES BIOLOGICALS, INC.
© Novozymes Biologicals, Inc.
Novo MAC Series with BioS™ Consortium
2006-43311-01

2. Novo MAC - technology so new it deserves a new brand
© Novozymes Biologicals, Inc.
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• The Novo brand represents the “best of the best”… premium products
made from the very best of the ever-expanding Novozymes’ culture
collection.
• The multi-action five strain Bacillus consortium (MAC) is referred to as the
BioS™ consortium.
• Compared to the market leading MSB blend, the new BioS™ Bacillus
spore blend (Novo MAC) exhibits higher levels of enzyme activity,
synergism among the chosen strains, and a broader range of aerobic and
anaerobic activity.
• The BioS™ blend excels in producing enzyme activities for multi-purpose
applications - amylase, cellulase, lipase and protease.

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Improved performance in applications
• Drain lines and grease traps
Faster degradation to eliminate build-ups of proteins, fats, oils,
grease, starches and toilet tissue.
• Septic and waste treatment
More effective waste degradation in septic systems to better
protect drainfield, avoid excessive pumping and eliminate odors
caused by volatile fatty acids.
• Bathroom cleaner and odor controller
Faster breakdown of residual soils leaving a visually cleaner
surface while eliminating odors at their source.

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The best technology on the market just got better
Microbes in Novo MAC breakdown organics faster than MSB strains
Respirometry was used to
monitor the effectiveness of
synthetic sludge degradation by
comparing BioS™ microbial
blend and the MSB microbial
blend as measured by oxygen
consumption.
The cumulative uptake is 74%
higher for the BioS™ microbial
blend, indicating far better
degradation activity.

5. The BioS™ microbial blend
demonstrates superior cellulase,
lipase and protease enzyme
production when compared to the
market leading MSB blend. Both
blends produce high levels of
amylase.
© Novozymes Biologicals, Inc.
after 24-48 hours BioS™
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Enzyme profiles of BioS™ consortium
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MSB
Cellulase Amylase Protease Lipase
Size of zones of clearing (mm)
Differentiated media on Petri plates visibly demonstrate breakdown of organic compounds by
producing a “zone of clearing” (hydrolysis) around each colony. A given quantity of microbial
culture is spotted onto a disk that is placed on a Petri plate containing a solid media (substrate)
that allows visualization of an enzyme reaction.
As the targeted enzyme is produced and diffuses throughout the media, a “zone of clearing” is
created where the substrate is broken down by the enzyme. The zones of clearing are measured
from the edge of a microbial colony outwards to the edge of the zone of clearing.

6. Enzyme profiles of BioS™ consortium - Cellulase
• Is the most abundant biological compound on earth
• Is present in stains, grime and organic deposits
The BioS™ consortium produces cellulase enzymes that break down this large-chain
insoluble molecule into much smaller water soluble, more readily biodegradable fragments.
CMC plate stained with Congo red
solution
© Novozymes Biologicals, Inc.
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BioS™ and MSB consortiums are each spotted onto
separate disks placed on a Petri plate containing a
carboxymethylcellulose (CMC) medium. As cellulase
is produced and excreted, the CMC is broken down.
After the plate is stained with a congo red solution,
the zone of clearing becomes visible around the
colony where the CMC has been broken down.
Zones of clearing
MSB
BioS™
The BioS™ consortium produces
significantly more cellulase when
compared to the MSB consortium.

7. Enzyme profiles of BioS™ consortium - Lipase
The BioS™ consortium shows a deeper Lipase plate
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• Fats, oils and grease (FOG) are major portions of soils and organic deposits.
• FOG is primarily composed of triglycerides. The BioS™ consortium produces a lipase
enzyme that breaks down triglycerides into its more basic biodegradable components.
BioS™ and MSB consortiums are each spotted
onto separate disks placed on a Petri plate
containing a lipase medium. As lipase is
excreted, the triglycerides are hydrolyzed and
the pH drops in the area where the break down
has occurred.
Zones of clearing
Zones of clearing
MSB BioS™
MSB
Zones of clearing
BioS™
MSB
BioS™
green color revealing that more
triglycerides were broken down, i.e. the
BioS™ consortium produced more
lipase enzyme, when compared to the
MSB consortium.

8. Enzyme profiles of BioS™ consortium - Protease
Proteases are enzymes that hydrolyze peptide bonds in proteins to break down the large
structure into smaller groups of amino acids.
• Due to the high degree of charged amino acids in proteins they are usually very sticky,
and the proteases produced by the BioS™ microbial blend help to reduce this stickiness by
breaking down the protein into more readily biodegradable parts.
Casein plate The BioS™ consortium produces a
Zones of clearing
© Novozymes Biologicals, Inc.
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BioS™ and MSB consortiums are each spotted
onto separate disks placed on a Petri plate
containing a casein skim milk medium. As
protease is produced and excreted, a zone of
clearing becomes visible where the skim milk
has been broken down.
MSB
BioS™
significantly higher level of protease
when compared to the MSB consortium.

9. Enzyme profiles of BioS™ consortium - Amylase
• Is present in most food substances
• Contributes to soiling by causing particles to stick more readily to surfaces
The amylase produced by the BioS™ consortium breaks down the starch into a more
biodegradable form, reducing the stickiness of particles and making them easier to remove.
© Novozymes Biologicals, Inc.
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BioS™ and MSB consortiums produce
considerable levels of amylase.
Zones of clearing
MSB BioS™
Starch plate stained with an iodine
solution
BioS™ and MSB consortiums are each spotted onto
separate disks placed on a Petri plate containing
a starch medium. As amylase is produced and
excreted, the starch is broken down.
After the Petri plate is stained with a iodine solution,
the zone of clearing becomes visible where the starch
has been broken down.

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Summary
The “Best of the Best” BioS™ microbial blend demonstrates superior
cellulase, lipase and protease enzyme production when compared to the
market-leading MSB blend for faster degradation of organics.
• Activity 74% higher than the market leading MSB consortium on simulated
drain sludge.
• Improved enzyme production means improved degradation and removal of
proteins, fats, oils, grease, starches and toilet tissue for better cleaning,
eliminating build-ups in drains and grease traps, and more effective septic
systems.
• They are safe to use as evidenced by acute oral, dermal sensitization, and
acute inhalation studies.