M Phil, Final seminar, Topic: Effect of Dietary Selenium supplementation on gross morphology, antioxidant status and biochemical composition of young goat

2. Special Thanks to Dr Nimra Bhatti, This presentation is being
published by her permission.
Dr. Mubasher Solangi, Research Fellow

3. Department of Veterinary Physiology and Biochemistry
Faculty of Animal Husbandry and Veterinary Sciences
Sindh Agriculture University, Tandojam
Effectof Dietary SeleniumYeast Supplementationon Gross
Morphology antioxidantstatus and Biochemical composition of
theOviduct on Young Goats
M.Phil Thesis Defense Seminar
Titled
By
Nimra bhatti
2k15-BP-99

4. Department of Veterinary Physiology and Biochemistry
Faculty of Animal Husbandry and Veterinary Sciences
Sindh Agriculture University, Tandojam
DR. MOOLCHAND MALHI
Associate Professor
DR. ALLAH BUX KACHIWAL
Professor
DR. REHANA BURIRO
Associate Professor

5. INTRODUCTION
TheFemale ReproductiveTract
Female reproductive tract consist of the Ovary ,oviduct (also called
Fallopian tube),body of the uterus, uterine horns, cervix, vagina
(Copulatory organ) and vulva .

6. INTRODUCTION
TheFemale ReproductiveTract
• The oviduct, a part of the female reproductive tract, is a tube-like
structure that connects the ovary to the uterus. The oviduct is consists of
• Infundibulum
• Ampulla
• Isthmus
With these three distinct structures, the oviduct serves as a passage that
transports gametes and the embryo as well as provides important
structural, environmental and nutritional support.

7. INTRODUCTION
Oviduct and oxidative stress
• Oviduct is a very complex and metabolically active
organ, the active metabolism in oviduct may
generate free radicals, if these free radicals are not
removed or deactivated by the time the condition is
developed known as oxidative stress.

8. Selenium as an antioxidant:
• Se is an integral part of Glutathione peroxidase (GSH-
Px), an antioxidant which helps repair cell damage

9. • Because Selenium is integral part of GPx that is
synthesized in oviductal tissue, would directly
depend upon selenium concentration that ultimately
comes through diet.
• We hypothesized that addition of Se in diet would
positively affect on oviductal growth by improving it
anti-oxidant status.
Hypothesis

10. Objectives
1. To evaluate the effect of dietary SY
supplementation on antioxidant status in oviduct
of goat.
2. To evaluate the effect of dietary SY
supplementation on biochemical composition in
ovidutal fluid of goat.
3. To evaluate the effect of dietary SY
supplementation on Histomorphology in oviduct of
goat

11.  The Experimental Animals:
10 young cross bred female goats (4 mo, 15.17 kg BW
Feeding
Concentrate 140 g/day, Hay and water adlib (Basal Diet)
Brief Methodology
Group B
(SY, n=5)
Se (0.3mg/kg diet)
Group A
(C, n=5)
Se (0 mg/kg diet)
 Se source: Synthetic organic form, Selenium Yeast
Selemax™, Biorigin®, Lençóis Paulista, São Paulo, Brazil
Experimental Design:
(Two groups)

12. Experimental Period, Slaughter and Sampling:
After slaughter isolation of
oviduct from rest of the
reproductive organ
Brief Methodology
Adaptation Experimental Period
Weeks

13. Gross
Morphology
Histo-Morphology
Length
Weight
Width
GPx Activity
SOD Activity in
oviductal fluid
and tissue
 Histological
architecture of
oviduct.
Thickness
Height of mucosal
folds
Height of ciliated cell
Thickness of t.m,
t.mus. t. s
biochemical
composition
Total protein
Glucose
Cholesterol
Total Lipid
from
oviductal
fluid
Antioxidant

14. Data Analysis
o Data was statically anatomized as Mean ± SEM
and differences were considered significant at P <
0.05 by one way ANOVA, Means were compared
by Tukey’s HSD (Statstix 10, USA, © statstix.com)
statistical software package

15. RESULTS

16. Gross Morphology

17. Effect of dietary SY supplementatioon on length
and weight of oviduct
Item
Groups
A B P- value
Right oviduct
Weight (g) 0.34 ± 0.1 0.44 ± 0.01 0.002
Length (cm) 11.97 ± 0.55 15.55 ± 0.35 0.001
Left oviduct
Weight (g) 0.35 ± 0.01 0.45 ± 0.01 0.001
Length (cm) 12.90 ± 0.36 16.05 ± 0.28 0.005
Goats fed on basal diet as control group (A) whereas in group B goats fed basal diet supplemented with selenium yeast at the dose
rate of 0.3mg/kg body weight for 10 weeks. Date is presented as Mean ± SE, significant difference was considered at P < 0.05.

18. Effect of dietary SY supplementatioon on length
of various parts of oviduct
Goats fed on basal diet as control group (A) whereas in group B goats fed basal diet supplemented with selenium yeast at the dose
rate of 0.3mg/kg body weight for 10 weeks. Date is presented as Mean ± SE, significant difference was considered at P < 0.05.
Item
Groups
A B P- value
Right oviduct (cm)
Isthmus 5.49 ± 0.27 7.04 ± 0.16 0.002
Ampulla 5.36 ± 0.27 6.96 ± 0.16 0.002
Infundibulum 1.12 ± 0.02 1.19 ± 0.05 0.63
Left oviduct (cm)
Isthmus 5.95 ± 0.19 7.42 ± 0.14 0.001
Ampulla 5.82 ± 0.15 7.30 ± 0.12 0.003
Infundibulum 1.17 ± 0.02 1.23 ± 0.04 0.380

19. Effect of dietary SY supplementatioon on width
of various parts of oviduct
Goats fed on basal diet as control group (A) whereas in group B goats fed basal diet supplemented with selenium yeast at the dose rate
of 0.3mg/kg body weight for 10 weeks. Date is presented as Mean ± SE, significant difference was considered at P < 0.05.
Item
Groups
A B P- value
Right oviduct (cm)
Isthmus 0.12 ± 0.02 0.20 ± 0.04 0.09
Ampulla 0.20 ± 0.00 0.26 ± 0.01 0.07
Infundibulum 0.80 ± 0.10 1.32 ± 0.17 0.43
Left oviduct (cm)
Isthmus 0.13 ± 0.12 0.18 ± 0.02 0.14
Ampulla 0.15 ± 0.03 0.21 ± 0.07 0.08
Infundibulum 0.50 ± 0.10 1.25 ± 0.21 0.12

20. Effect of dietary SY plementatioon on
biochemical values of oviduct flushing
Goats fed on basal diet as control group (A) whereas in group B goats fed basal diet supplemented with selenium yeast at the dose
rate of 0.3mg/kg body weight for 10 weeks. Date is presented as Mean ± SE, significant difference was considered at P < 0.05.
Item
Groups
A B P- value
Right oviduct
Glucose (mg/dl) 54.36 ± 0.92 60.36 ± 1.31 0.02
Protein (g/dl) 3.67 ± 0.21 5.14 ± 0.11 0.04
Cholesterol (mg/dl) 41.29 ± 0.62 38.62 ± 0.46 0.02
HDL (mg/dl) 11.22 ± 0.69 17.47 ± 0.51 0.01
Triglyceride (mg/dl) 57.20 ± 0.34 37.88 ± 0.60 0.01
LDL (mg/dl) 41.50 ± 1.17 28.41 ± 1.02 0.01

21. Effect of dietary SY supplementatioon on
biochemical values of oviduct flushing
Goats fed on basal diet as control group (A) whereas in group B goats fed basal diet supplemented with selenium yeast at the dose
rate of 0.3mg/kg body weight for 10 weeks. Date is presented as Mean ± SE, significant difference was considered at P < 0.05.
Item
Groups
A B P- value
Left oviduct
Glucose (mg/dl) 52.70 ± 0.83 57.85 ± 1.09 0.02
Protein (g/dl) 4.11 ± 0.36 5.93 ± 0.09 0.008
Cholesterol (mg/dl) 44.26 ± 0.33 38.78 ± 0.40 0.005
HDL (mg/dl) 10.75 ± 0.92 21.71 ± 1.08 0.001
Triglyceride (mg/dl) 57.23 ± 0.34 47.01 ± 0.46 0.001
LDL (mg/dl) 44.94 ± 0.92 26.46 ± 1.38 0.004

22. Effect of dietary SY supplementatioon on Antioxidant
status of oviduct
Goats fed on basal diet as control group (A) whereas in group B goats fed basal diet supplemented with selenium yeast at the dose
rate of 0.3mg/kg body weight for 10 weeks. Date is presented as Mean ± SE, significant difference was considered at P < 0.05.
Item Groups
A B P- value
Flushing
GPx (U/ml) 18.00 ± 0.20 26.26 ± 0.75 0.005
SOD (U/ml) 3.90 ± 0.52 9.11 ± 0.24 0.008
Tissue
GPx (U/ml) 69.23 ± 0.60 90.80 ± 2.00 0.005
SOD (U/ml) 22.10 ± 1.23 40.82 ± 0.86 0.002

23. Histology
Micrograph of cs of isthmus of goat showing isthmic folds and histological membranes (H&E, 10x).
Goats fed on basal diet as control group (A) whereas in group B goats fed basal diet supplemented with
selenium yeast at the dose rate of 0.3mg/kg body weight for 10 weeks. Date is presented as Mean ± SE,
significant difference was considered at P < 0.05.

24. Effect of dietary SY supplementatioon on
histomorphometry of Isthmus
Goats fed on basal diet as control group (A) whereas in group B goats fed basal diet supplemented with selenium yeast
at the dose rate of 0.3mg/kg body weight for 10 weeks. Date is presented as Mean ± SE, significant difference was
considered at P < 0.05.
Item (µm) Groups
A B P- value
Thickness of primary fold 48.50 ± 2.50 150.00 ± 3.0 0.01
Thickness of secondary fold 79.50 ± 1.50 96.50 ± 2.50 0.06
Height of mucosal fold 213.50 ± 6.50 481.0 ± 3.0 0.03
Thickness of mucosal fold at tip 119.00 ± 6.00 99.50 ± 4.50 0.01
Thickness of mucosal fold at base 78.50 ± 3.50 206.5 ± 7.50 0.04
Height of ciliated cell 37.50 ± 1.50 42.00 ± 1.00 0.04
Thickness t. mucosa 26.00 ± 1.20 34.50 ± 1.50 0.02
Thickness of t. muscularis 41.00 ± 1.02 49.00 ± 4.0 0.32
Thickness of t. serosa 24.50 ± 2.50 29.50 ± 1.50 0.64

25. Histology
Micrograph of cs of ampula of goat showing ampulary folds and histological membranes (H&E, 10x).
Goats fed on basal diet as control group (A) whereas in group B goats fed basal diet supplemented with selenium yeast
at the dose rate of 0.3mg/kg body weight for 10 weeks. Date is presented as Mean ± SE, significant difference was
considered at P < 0.05.

26. Effect of dietary SY supplementatioon on
histomorphometry of ampulla
Goats fed on basal diet as control group (A) whereas in group B goats fed basal diet supplemented with
selenium yeast at the dose rate of 0.3mg/kg body weight for 10 weeks. Date is presented as Mean ± SE,
significant difference was considered at P < 0.05.
Item (µm) Groups
A B P- value
Thickness of primary fold 87 ± 1.00 177.5 ± 2.50 0.02
Thickness of secondary fold 90.5 ± 8.50 85.5 ± 12.50 0.35
Height of mucosal fold 118.5 ± 6.5 114.5 ± 6.05 0.95
Thickness of mucosal fold at tip 124.51 ± 0.12 174.3 ± 0.06 0.03
Thickness of mucosal fold at base 28.50 ± 1.50 42.50 ± 4.50 0.03
Height of ciliated cell 24.37 ± 2.32 58.23 ± 2.00 0.01
Thickness t. mucosa 63.29 ± 1.55 93.52 ± 2.5 0.03
Thickness of t. muscularis 28.50 ± 3.5 38.10 ± 2.84 0.08
Thickness of t. serosa 87 ± 1.00 107.5 ± 2.50 0.21

27. Conclusion
The SY supplementation in the diet of goat improved
histo-morphological characteristics in the ampulla and
isthmus oviduct.
The SY supplementation improved biochemical
characteristics in oviductal fluid by increasing glucose
and protein concentrations and decreasing lipid
metabolites.
The SY supplementation enhances antioxidant status
in oviductal fluid and oviductal tissue which protect
the oviduct from oxidative stress.

28. Suggestions
The SY supplementation produced positive effects
on oviductal growth and may be used as supplement in
goat’s diet to ensure the normal level of selenium
present in diet.
Evaluation should be performed to fully perceive the
whole mechanism of SY on the oviductal growth in
ruminants and other animals.

29. drmubasher@hotmail.com
+92 333 2799 754