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Nanotechnology: Application in crop nutrition
Presented by:
Sangavi K K
ALH5059
UNIVERSITY OF AGRICULTURAL SCIENCES,
BENGALURU
COLLEGE OF AGRICULTURE, HASSAN
1
Overview
Review of literature
Introduction
Nano fertilizer
Research work
Future prospects
Conclusion
Reference
2
Review of literature
• NASS(2013) Nanotechnology in Agriculture
:Scope and current relevance .Policy paper
No.63,New Delhi.
• Tarafdar,J.C.(2012) Perspectives of
nanotechnological applications for crop
production . NAAS News 12,8-11.
• Drexler,K.E.(1986)Engines of creation :The
Coming Era of Nanotechnology . Random house
New York.
3
• Tarafdar ,J.C.,Raliya, R . and Rathore, I.(2012)
Microbial synthesis of phosphorous nano
particles from tri -calcium phosphate using
Aspergillus tubingenesis TFR-5. Journal of
Bionanoscience 6 ,84-89.
• Hett A (2004) Nanotechnology .Small Matter
,Many Unknown .SwissRe Zurich .
4
Nanotechnology
 The prefix ‘nano’ derived from the Greek word
that means ‘dwarf’ and ‘technology’ is the
application of practical sciences to commerce .
 Foundation of nano science was first established
by Nobel laureate Richard Feyman.
 The term nanotechnology was coined by Norio
Taniguchi of the University of Tokyo in 1974 .
5
 Nanotechnology therefore refers to ‘the creation
and utilization of materials, devices and systems
through the control of their properties and
structure at a nano metric scale’.
 The word nano is used to refer to 10-9 or the
billionth part of one metre.
 Nano particles generally used for materials of
sizes between 1 to 100 nm.
6
7
Properties
 Smaller size
 High surface area to volume ratio.
 Slow release
 High mobility and more efficient .
8
9
Nano terms
• Nano scale
• Nano science
• Nano technology
-Natural
-Incidental
-Engineered
10
Synthesis of Nanoparticles
Top down Bottom up
11
12
Nano fertilizers
Need
 High prone to losses of fertilizer.
 Pollution to environment.
 Low nutrient use efficiency.
 Increasing population and decreasing
productivity.
13
Nutrient Efficiency Cause of low efficiency
Nitrogen 30-35 % Immobilization, volatilization,
denitrification,
Leaching
Phosphorus 15-20% Fixation in soils Al – P, Fe – P, Ca – P
Potassium 35-40% Fixation in clay - lattices
Sulphur 8-10% Immobilization, Leaching with water
Micro
nutrients (Zn,
Fe, Cu, Mn, B)
2-5% Fixation in soils
14
15
Nano fertilizer is a smart way to release
plant nutrients gradually and in controlled
manner.
Reduce quantity of fertilizer used .
Control eutrophication and pollution of
water resource.
Ultrahigh absorption rate increased
production ,photosynthesis and significant
leaf SA expansion.
16
17
18
Mode of action
19
Characterisation of nanofertilizers
20
Transmission
electron microscopy
Scanning electron
microscopy
Dynamic light
scattering
21
TEM image of nanoparticles SEM image of nanoparticles
Iron oxide
Zinc oxide
Nano porous zeolites
• Zeolites are naturally occurring minerals
honeycomb like structure arrangement of Al and Si
in 3-dimensional framework creates channels and
voids that are in nano scale.
• High specific surface area, CEC and highly
selective towards macronutrient K+ and NH4+.
• These essential minerals can be exchanged into
zeolite exchange site, where nutrient can slowly
release for plant uptake, so reduce runoff, leaching
and environmental pollution.
2222
23
Effects of nanofertilizers
• Increase nutrient use efficiency :
Encapsulation of fertilizer with nano particles
Slow delivery
Smart delivery system
Nano biosensor
• Increase in yield
• Precision farming
24
Constraints
25
Environmental pollution.
Respiratory disorder and carcinogenic.
Some ill effects on plant system
Research work
 Foliar application of nano phosphorous as
fertilizer (640 mg/ha) and soil application (80
kg/ha) yield equally in cluster bean and pearl
millet under arid condition.
(Tarafdar et al.,2012)
 The application of nano-urea can save up to
12.4-41.7%of nitrogen application to the soil.
(Huang et al.,2015)
26
27
Treatments No. of Grains/Spike 100-Grain weight
(g)
Yield/Pot (g)
0 ppm 18.5 3.35 7.18
25 ppm 29.0 4.66 13.25
50 ppm 22.0 4.53 12.45
75 ppm 25.0 4.40 10.40
100 ppm 22.3 4.43 10.36
125 ppm 22.5 3.94 9.90
150 ppm 11.5 3.78 9.73
CD at 5% 3.52 0.25 1.77
RDF: 90-60-40 kg/ha Jhanzab et al., 2015
Effect of silver nano particles on yield attributes of
wheat
28
Prasad etal 2012
a) Higher root growth of peanut plant after nanoscale ZnO treatment (1000ppm).
The plants were uprooted after 110 days
b) Higher plant growth after nanoscale ZnO treatment (1000 ppm), after 110days
Effect of nano ZnO on uptake of zinc by leaf and kernel of
peanut
29
Treatment Zinc content (ppm)
2008–2009 (Rabi season)
Leaf
(post harvest) Kernel
T1 = NPK
(Control) 22.31 21.84
T2 = NPK + ZnSO4
(chelated @ 30g/15 L)
31.46∗ 28.32∗
Zinc content (ppm)
2009–2010(Rabi season)
Leaf Kernel
(post harvest)
22.81 20.46
32.36∗ 29.21∗
T3 = NPK + ZnO
(Nano @ 2g/ 15 L) 44.80∗∗ 40.20∗∗ 41.83∗∗ 39.90∗∗
CD@ 5% 1.50 1.36 1.46 1.35
Prasad et al., 2012
Effect of zinc nanofertilizer on pearl millet
Treatment Shoot length
(cm)
Root length
(cm)
Dry biomass
(kg/ha)
Grain yield
(kg/ha)
Control 152 58.6 5192 1065
Ordinary ZnO 158 60.9 5214 1217
Nano ZnO 175 61.1 5841 1467
CD @ 5% 0.10 0.14 52.2 17.6
Tarafdar et al., 2014ZnO NP size: 18.5nm
Foliar application rate @ 16 litre/ha at 10ppm conc.
30
Treatments Acid
phosphatase
(EU× 10-4)
Alkaline
phosphatase
(EU× 10-4)
Phytase
(EU× 10-2)
Control 9.1 4.7 0.9
Ordinary ZnO 14.1 6.2 2.2
Nano ZnO 16.1 7.6 3.8
CD @ 5% 1.4 0.8 0.5
P-solubilising enzyme activity in rhizosphere of 6 week old
pearl millet
Tarafdar et al., 2014
EU : Enzymatic Units
31
From left to right: Treated with fertilizer and nano-sized hydroxyapatite (nHA),
treated with fertilizer and regular P, treated with fertilizer without P, and treated with
tap water only.
Crop: Soybean
Liu and Lal, 2014
32
Controlled release fertilizer of zinc encapsulated by hollow
core shell (nano size)
Yuvaraj and Subramanian, 2015Hollow core size: 155 nm 33
Effect of copper nanoparticles on
root growth of wheat
0.4ppm Control
Micrograph of root
indicating absorption
of Cu-NPs
Hafeez et al., 2015
34
Effect of zinc nanofertilizer on growth and
yield of pearl millet crop
Tarafdar et al., 2014
Treatments Root
Leng
th
(cm)
Roo
t
Are
a
(cm
2)
Total
chlorop
hyll
content
(µg-1)
Grain
Yield
(kg
/ha)
Dry
biomass
(kg
/ha)
Control 58.6 60.1 30.3 1065 5192
ZnSO4 60.9 63.8 31.5 1217 5214
Nano Zn 61.1 74.7 37.7 1467 5841
CD at 5% 0.14 0.17 0.46 17.6 52.2
Foliar spray after two weeks of
germination@10mg/l
35
Future prospects
Physiological explanation of mechanism of
uptake and translocation by plants
Influence of nanoparticles in rhizosphere and
on root surface
Minimising the residual effect
Lab to land
36
Conclusion
 Nano-fertilizers have potential to increase
crop productivity through slow or controlled
delivery.
 Due to their small size and target specificity,
they increase the use efficiency of the
fertilizer, which are applied in nanoparticle
form.
 If limitations are encountered it will become
a revolutionizing technology.
37
Reference
• Soil science an introduction -ISSS
• Nanotechnology and plant sciences – Manzer H
Siddiqui et al.,
• Crop nutrition management with nano fertilizers
–Tulasi Guru et al.,
• Internet
38
THANK YOU

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NANOTECHNOLOGY: APPLICATION IN CROP NUTRTION

  • 1. Nanotechnology: Application in crop nutrition Presented by: Sangavi K K ALH5059 UNIVERSITY OF AGRICULTURAL SCIENCES, BENGALURU COLLEGE OF AGRICULTURE, HASSAN 1
  • 2. Overview Review of literature Introduction Nano fertilizer Research work Future prospects Conclusion Reference 2
  • 3. Review of literature • NASS(2013) Nanotechnology in Agriculture :Scope and current relevance .Policy paper No.63,New Delhi. • Tarafdar,J.C.(2012) Perspectives of nanotechnological applications for crop production . NAAS News 12,8-11. • Drexler,K.E.(1986)Engines of creation :The Coming Era of Nanotechnology . Random house New York. 3
  • 4. • Tarafdar ,J.C.,Raliya, R . and Rathore, I.(2012) Microbial synthesis of phosphorous nano particles from tri -calcium phosphate using Aspergillus tubingenesis TFR-5. Journal of Bionanoscience 6 ,84-89. • Hett A (2004) Nanotechnology .Small Matter ,Many Unknown .SwissRe Zurich . 4
  • 5. Nanotechnology  The prefix ‘nano’ derived from the Greek word that means ‘dwarf’ and ‘technology’ is the application of practical sciences to commerce .  Foundation of nano science was first established by Nobel laureate Richard Feyman.  The term nanotechnology was coined by Norio Taniguchi of the University of Tokyo in 1974 . 5
  • 6.  Nanotechnology therefore refers to ‘the creation and utilization of materials, devices and systems through the control of their properties and structure at a nano metric scale’.  The word nano is used to refer to 10-9 or the billionth part of one metre.  Nano particles generally used for materials of sizes between 1 to 100 nm. 6
  • 7. 7
  • 8. Properties  Smaller size  High surface area to volume ratio.  Slow release  High mobility and more efficient . 8
  • 9. 9
  • 10. Nano terms • Nano scale • Nano science • Nano technology -Natural -Incidental -Engineered 10
  • 11. Synthesis of Nanoparticles Top down Bottom up 11
  • 12. 12
  • 13. Nano fertilizers Need  High prone to losses of fertilizer.  Pollution to environment.  Low nutrient use efficiency.  Increasing population and decreasing productivity. 13
  • 14. Nutrient Efficiency Cause of low efficiency Nitrogen 30-35 % Immobilization, volatilization, denitrification, Leaching Phosphorus 15-20% Fixation in soils Al – P, Fe – P, Ca – P Potassium 35-40% Fixation in clay - lattices Sulphur 8-10% Immobilization, Leaching with water Micro nutrients (Zn, Fe, Cu, Mn, B) 2-5% Fixation in soils 14
  • 15. 15
  • 16. Nano fertilizer is a smart way to release plant nutrients gradually and in controlled manner. Reduce quantity of fertilizer used . Control eutrophication and pollution of water resource. Ultrahigh absorption rate increased production ,photosynthesis and significant leaf SA expansion. 16
  • 17. 17
  • 19. 19
  • 20. Characterisation of nanofertilizers 20 Transmission electron microscopy Scanning electron microscopy Dynamic light scattering
  • 21. 21 TEM image of nanoparticles SEM image of nanoparticles Iron oxide Zinc oxide
  • 22. Nano porous zeolites • Zeolites are naturally occurring minerals honeycomb like structure arrangement of Al and Si in 3-dimensional framework creates channels and voids that are in nano scale. • High specific surface area, CEC and highly selective towards macronutrient K+ and NH4+. • These essential minerals can be exchanged into zeolite exchange site, where nutrient can slowly release for plant uptake, so reduce runoff, leaching and environmental pollution. 2222
  • 23. 23
  • 24. Effects of nanofertilizers • Increase nutrient use efficiency : Encapsulation of fertilizer with nano particles Slow delivery Smart delivery system Nano biosensor • Increase in yield • Precision farming 24
  • 25. Constraints 25 Environmental pollution. Respiratory disorder and carcinogenic. Some ill effects on plant system
  • 26. Research work  Foliar application of nano phosphorous as fertilizer (640 mg/ha) and soil application (80 kg/ha) yield equally in cluster bean and pearl millet under arid condition. (Tarafdar et al.,2012)  The application of nano-urea can save up to 12.4-41.7%of nitrogen application to the soil. (Huang et al.,2015) 26
  • 27. 27 Treatments No. of Grains/Spike 100-Grain weight (g) Yield/Pot (g) 0 ppm 18.5 3.35 7.18 25 ppm 29.0 4.66 13.25 50 ppm 22.0 4.53 12.45 75 ppm 25.0 4.40 10.40 100 ppm 22.3 4.43 10.36 125 ppm 22.5 3.94 9.90 150 ppm 11.5 3.78 9.73 CD at 5% 3.52 0.25 1.77 RDF: 90-60-40 kg/ha Jhanzab et al., 2015 Effect of silver nano particles on yield attributes of wheat
  • 28. 28 Prasad etal 2012 a) Higher root growth of peanut plant after nanoscale ZnO treatment (1000ppm). The plants were uprooted after 110 days b) Higher plant growth after nanoscale ZnO treatment (1000 ppm), after 110days
  • 29. Effect of nano ZnO on uptake of zinc by leaf and kernel of peanut 29 Treatment Zinc content (ppm) 2008–2009 (Rabi season) Leaf (post harvest) Kernel T1 = NPK (Control) 22.31 21.84 T2 = NPK + ZnSO4 (chelated @ 30g/15 L) 31.46∗ 28.32∗ Zinc content (ppm) 2009–2010(Rabi season) Leaf Kernel (post harvest) 22.81 20.46 32.36∗ 29.21∗ T3 = NPK + ZnO (Nano @ 2g/ 15 L) 44.80∗∗ 40.20∗∗ 41.83∗∗ 39.90∗∗ CD@ 5% 1.50 1.36 1.46 1.35 Prasad et al., 2012
  • 30. Effect of zinc nanofertilizer on pearl millet Treatment Shoot length (cm) Root length (cm) Dry biomass (kg/ha) Grain yield (kg/ha) Control 152 58.6 5192 1065 Ordinary ZnO 158 60.9 5214 1217 Nano ZnO 175 61.1 5841 1467 CD @ 5% 0.10 0.14 52.2 17.6 Tarafdar et al., 2014ZnO NP size: 18.5nm Foliar application rate @ 16 litre/ha at 10ppm conc. 30
  • 31. Treatments Acid phosphatase (EU× 10-4) Alkaline phosphatase (EU× 10-4) Phytase (EU× 10-2) Control 9.1 4.7 0.9 Ordinary ZnO 14.1 6.2 2.2 Nano ZnO 16.1 7.6 3.8 CD @ 5% 1.4 0.8 0.5 P-solubilising enzyme activity in rhizosphere of 6 week old pearl millet Tarafdar et al., 2014 EU : Enzymatic Units 31
  • 32. From left to right: Treated with fertilizer and nano-sized hydroxyapatite (nHA), treated with fertilizer and regular P, treated with fertilizer without P, and treated with tap water only. Crop: Soybean Liu and Lal, 2014 32
  • 33. Controlled release fertilizer of zinc encapsulated by hollow core shell (nano size) Yuvaraj and Subramanian, 2015Hollow core size: 155 nm 33
  • 34. Effect of copper nanoparticles on root growth of wheat 0.4ppm Control Micrograph of root indicating absorption of Cu-NPs Hafeez et al., 2015 34
  • 35. Effect of zinc nanofertilizer on growth and yield of pearl millet crop Tarafdar et al., 2014 Treatments Root Leng th (cm) Roo t Are a (cm 2) Total chlorop hyll content (µg-1) Grain Yield (kg /ha) Dry biomass (kg /ha) Control 58.6 60.1 30.3 1065 5192 ZnSO4 60.9 63.8 31.5 1217 5214 Nano Zn 61.1 74.7 37.7 1467 5841 CD at 5% 0.14 0.17 0.46 17.6 52.2 Foliar spray after two weeks of germination@10mg/l 35
  • 36. Future prospects Physiological explanation of mechanism of uptake and translocation by plants Influence of nanoparticles in rhizosphere and on root surface Minimising the residual effect Lab to land 36
  • 37. Conclusion  Nano-fertilizers have potential to increase crop productivity through slow or controlled delivery.  Due to their small size and target specificity, they increase the use efficiency of the fertilizer, which are applied in nanoparticle form.  If limitations are encountered it will become a revolutionizing technology. 37
  • 38. Reference • Soil science an introduction -ISSS • Nanotechnology and plant sciences – Manzer H Siddiqui et al., • Crop nutrition management with nano fertilizers –Tulasi Guru et al., • Internet 38 THANK YOU