The document summarizes the structure of the thorax and appendages of insects. It discusses that the thorax is comprised of three segments - prothorax, mesothorax, and metathorax. Each segment contains a pair of legs and the mesothorax and metathorax together form the pterothorax which contains the fore and hind wings. It also describes the different types of wing coupling mechanisms seen in insects like hamulate, amplexiform, frenate and jugate.
Structure and types of insect legs and identification of insect legs, Modification in insect legs - Cursorial leg(running leg), Ambulatorial leg(walking leg), Saltatorial leg(jumping leg), Scansorial leg(climbing leg), Fossorial leg(digging leg), Natatorial leg(swimming leg), Raptorial leg(grasping leg), Basket – like leg, Sticking leg, Foragial leg, Prolegs or False legs or Pseudolegs
Orthoptera is an order of insects that comprises the grasshoppers, locusts and crickets, including closely related insects such as the katydids and wetas. The order is subdivided into two suborders: Caelifera – grasshoppers, locusts and close relatives; and Ensifera – crickets and close relatives.
Structure and types of insect legs and identification of insect legs, Modification in insect legs - Cursorial leg(running leg), Ambulatorial leg(walking leg), Saltatorial leg(jumping leg), Scansorial leg(climbing leg), Fossorial leg(digging leg), Natatorial leg(swimming leg), Raptorial leg(grasping leg), Basket – like leg, Sticking leg, Foragial leg, Prolegs or False legs or Pseudolegs
Orthoptera is an order of insects that comprises the grasshoppers, locusts and crickets, including closely related insects such as the katydids and wetas. The order is subdivided into two suborders: Caelifera – grasshoppers, locusts and close relatives; and Ensifera – crickets and close relatives.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
4. THORAX
Middle tagma
Three segmented - pro, meso and meta
Meso and meta thorax with wing are called as
Pterothorax
Thorax is made of three scleritic plates
1. dorsal body plate - Tergum or Nota
2. ventral body plate (Sterna)
3. lateral plate (Pleura)
5. THORACIC NOTA
3 segments - pronotum, mesonotum & metanotum
respectively
Pronotum - undivided & Saddle shaped in grass hopper,
Shield like in cockroach
Pterothoracic notum - have 3 transverse sutures
(Antecostal, Pre scutal and Scuto-scutellar)
&5 tergites(Acrotergite, Prescutum, Scutum, Scutellum
and Post-scutellum)
6. THORACIC STERNA
Vental body plate - prosternum,
mesosternum and metasternum
Made up of a segmental plate called
Eusternun and a intersternite called Spinasternum
Eusternum - made of three sclerites - presternum,
basisternum and sternellum
7. THORACIC PLEURA
Lateral body wall between notum & sternum
Selerites of pleuron is called as pleurites
fuse to form Pleural plate
Pleural plate is divided into anterior episternum
and posterior epimeron by Pleural suture
Pterothoracic pleuron provides space for articulation of wing
and
Two pairs of spiracles are also present in the mesopleuron and
metapleuron.
8. ABDOMEN
Third and posterior tagma
This tagma is made up of 9-11 Uromeres
(segments) and is highly flexible
abdominal segments are interconnected by a
membrane called conjunctiva
Each abdominal segment is made up of only two
sclerite
1. Tergum 2. Sternum
9. ABDOMEN
Eight pairs of spiracles in - first eight
abdominal segments
in addition to a pair of tympanum in the first
abdominal segment
Eight and ninth segments - female genital
structure
Ninth segment - male genital structure.
Cerci
11. • Insects have three pairs of legs, One pair each in
pro-, meso- and metathorax - ‘Hexapods’
• Each leg consists of six segments, articulating with
each other by mono-or-dicondylic articulations set
in a membrane, the corium.
LEGS
13. • Leg pads - walking on smooth surface
• Claws give needed grip - walking on rough surface
• When one structure is used, the other is bent
upwards.
14. Reduction of legs (Adult stage)
(i) Nymphalid butterflies:
In males the tarsus and pretarsus of foreleg are lacking
In females the fore tarsus consists of very short tarsomeres
(ii) Female coccids:
Sedentary - held in position by the stylets. The legs are
reduced.
(iii) Female stylopids:
Legs absent and are parasitic
15. (i) Leaf miners of Lepidoptera, Coleoptera and Sawflies
(ii) Parasitic larvae of Hymenoptera and Strepsiptera
(iii) Grubs of blister beetles
(iv) In Hymenoptera - larvae fed by the adults are apodous
Reduction in leg (Larval stage)
16. Based on the habitat and food habit
1. Walking/ Ambulatorial leg
2. Running / Cursorial leg
3. Jumping / Saltatorial leg
4. Grasping / Raptorial leg
5. Digging / Burrowing / Fossorial leg
6. Swimming / Natatorial leg
7. Clinging / Scansorial leg
8. Honey bee’s legs/ Foragial leg
Types or modifications of legs
18. • Each wing is a hollow extension of the body wall
• It arises dorsolaterally between the pleura and
nota of meso and metathoracic segments.
• The pleural plates of the thoracic segments -
support the bases of the paranotal lobes
Wing development in modern insects
• Adult insects - fully developed wings
• In holometabolus larvae - develop internally
• In hemimetabolous larvae - visible as external pads
• Mayflies have two winged stages viz., subimago and
imago
19. Areas of the wing
• The flexion and fold-lines divide the wing into
different areas.
• The region containing the bulk of the veins in front of
the claval furrow is called remigium.
• The area behind the claval furrow is called clavus
• In hind wings in which this area is greatly expanded
and known as vannus & the fold – vannal fold / plica
vannalis.
• The jugum is cut off by the jugal fold / plica jugalis if
it is present
20. Margins and angles
• triangular in shape – 3 sides & 3 angles
• anterior margin strengthened by the costa - coastal
margin
• lateral margin - apical margin
• posterior margin - anal margin
• angle of attachment to the thorax - humeral angle.
• angle between the coastal and apical margins - apical
angle
• angle between apical and anal margins - anal angle.
Humeral
angle
Costal
Margin
Apical
angle
Apical
margin
Anal angle
Anal
margin
Claval
furrow
Juga
fold
21. Pterostigma
• Pigmented spot on the anterior margin of the wing
• Present on both pairs of wings in Odonata
• In the forewings of many Hymenoptera, Psocoptera, Mecoptera an
Neuroptera.
• In Odonata, - it reduce the wing flutter during gliding and thereb
increases the gliding speed.
22. Wing membrane
• The wing membrane is semitransparent and exhibits
iridescence
• Sometimes wings are patterned by pigments in the
epidermal cells eg. mecoptera and tephritidae
• In insects with hardened forewings such as
Orthoptera and Coleoptera, the whole wing is
pigmented
23. higher pterygote insects, fore & hind wings -
coupled together as a unit
both pairs move synchronously
Types of wing coupling
1. Hamulate :
• A row of small hooks - on the
coastal margin of the hind
wing - hamuli.
• engage the folded posterior
edge of fore wing.
• e.g. bees.
WING COUPLING
24. 2. Amplexiform :
• the simplest form of wing coupling
• A linking structure is absent.
• Coupling is achieved by broad overlapping of adjacent
margins.
• e.g. butterflies.
25. 3. Frenate :
There are two sub types. e.g. Fruit sucking moth.
i Male frenate :
• Hindwing bears near the base of the coastal margin
a stout bristle called frenulum
• held by a curved process, retinaculum arising from
the subcostal vein found on the surface of the
forewing
26. ii. Female frenate :
Hindwing bears near the base of the costal margin
a group of stout bristle (frenulum)
engages there in a retinaculum formed by a patch
of hairs near cubitus.
27. 4. Jugate :
Jugam of the forewings are lobe like and it is locked
to the coastal margin of the hind wings.
e.g. Hepialid moths.