The Molisch test is used to identify the presence of carbohydrates. It was developed by the Austrian botanist Hans Molisch. The test involves adding a carbohydrate sample to Molisch reagent, which is a mixture of ethanol, phenol, and alpha-naphthol. Concentrated sulfuric acid is then slowly added down the side of the test tube. If a purple ring forms at the junction of the two liquids, it indicates the presence of a carbohydrate due to a chemical reaction between the carbohydrate and components of the Molisch reagent. The test is positive for most monosaccharides, disaccharides, oligosaccharides, and polysaccharides.

1. MOLISCH TEST
HIHISTORY:
This Test Named After The Name Of Its Dicoverer AUSTRIAN-
BONTANIST HANS-MOLISH

2. PPPPURPOSEP
● The Purpose Of This Test Is To Identify The
Carbohydrates Almost All The CARBS Show This
Test Positive
● MONOSACCHARIDES Readily Gives This Test
● DIA,OLIGO And POLYSACCHARIDES(First
Hydrolyzed To Monosaccharides) Takes Some Time
To Show Positive Result
● GLYCOPROTEINS Also Show This Test positive
● TRIOSE NOT SHOW POSITIVE RESULT

3. COMPOSITION
❏In Order Perform This Test First
Of All We Prepare The
MOLISCH REAGENT
❏The Molisch Reagent Is Also
Known As The ETHANOLIC ⍶
NAPHTHOL

4. MOLISCH
REAGENT
1. 95% ETHANOL
2. PHENOL ( Mostly
Used Is ⍶ -
NAPHTHOL In Some
Cases Resorcinol And
Thymol)
Their Mixture is
Molisch Reagent

5. Procedure
1. In Order Identify Carbohydrates
First Makes Its Solution
2. Like Glucose , Make Its Solution With
Distilied Water(take 2ml solution)
3. Add The Molisch Reagent(6 drops)
4. Add Conc.H2SO4 Along The Wall Of
Test-tube( few dropds)
5. followin reaction occur in test-tube As
Shown In Figure

6. CHEMISTRY
●As The Acid Cause Dehydration In
Sugar And Make The FURFURAL
●Which React With Tow Molecules Of
NAPHTHOL As Shown Above
●And make a purple colour dye
●Which shows that CARBOHYDRATES
are IDENTIFIED

7. MOLISCH TEST RESULT IS POSITIVE
THE PURPULE RING IS
FORMED ,WHICH
REMAINED UNTIL TEST-
TUBE NOT SHAKE .THAT
IS CONFORMATION OF
PRESENCE OF CARBS IN
SOLUTION