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Lecture 6:
EVE 161:

Microbial Phylogenomics
Lecture 6:
Era II: Culture Independent rRNA
UC Davis, Winter 2016
Instructors: Jonathan Eisen & Holly Ganz
Where we are going and where we have been
• Previous lecture:
!5. Modern view of Tree of Life
• Current Lecture:
!6. Era II: rRNA from environment
• Next Lecture:
!7: Era II: rRNA PCR
!2
• Problem set on archaea vs. eukaryote phylogeny papers
will be posted today or emailed
!3
Paper #1
!4
Lane et al. Abstract
!5
Background: Why Sequences?
!6
!7
Background: Why Sequences?
Background: Why rRNA
!8
!9
Background: Why rRNA
Methods: How Get RNA
!10
Methods: How Make Primers
!11
Methods: Reverse Transcriptase
!12
Methods: Nucleotides
!13
Methods: Sequencing
!14
Results and Discussion
!15
Priming
!16
Priming
!17
Priming
!18
Errors
!19
Errors
!20
Alignment
!21
Phylogeny
!22
!24
!25
!26
!27
!28
!30
!31
!32
!33
!34
!35
!36
!37
!38
`
!39
!40
!41
!42
!43
!44
!45
!46
!47
!48
!49
!50
!51
!52
!53
!54
!55
!56
!57
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!62

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