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MEDICAL LABORATORY
Aveen rasul
clinical biochemistry department
what’s medical laboratory?
▪ Medical laboratory is a place where tests are done
on clinical specimens and samples in order to get
information about the health of a patient as
pertaining to the diagnosis , treatment , and
prevention of disease .
Job opportunities :
▪ Generalist : a medical laboratory scientist that works
in all laboratory departments .
▪ Specialist : a medical laboratory scientist that has had
three years experience in a particular department in
addition to passing a specialist exam . This
professional will work only in this department .
▪ Supervisor : a medical laboratory scientist that has
risen in the ranks of the laboratory to become the
head of particular department .
Basic equipments used in a medical
laboratory :
Departments in medical laboratory :
▪ Clinical chemistry ( Biochemistry )
▪ Hematology
▪ Pathology
▪ Microbiology
▪ immunology
▪ Blood banking
▪ Molecular technique
Clinical biochemistry department :
▪ Biochemistry performs most chemical analysis
includes hormones , enzyme , electrolytes , and
drug levels in your blood stream , urine or other
body fluids .
▪ It’s Subdevided ito :
▪ Biochemical analysis
▪ immunochemistry
▪ Toxicology
Some Tests and instruments in
biochemistry laboratory :
▪ Spectrophotometer
▪ Biochemistry analyzer
▪ ELISA
▪ Blood gas analyzer
▪ Urine analyzer
▪ Ph meter
▪ Electrolyte analyzer
▪ Gel electrophoresis
instrument..
▪ Renal function test (RFT)
▪ Liver function test (LFT)
▪ HbA1c , glucose , GTT
▪ Electrolyte
▪ Amylase and lipase
▪ Tumor markers
▪ Vitamin D
▪ Arterial blood gases
▪ Lipid profile
▪ Hormone
▪ Thyroid functions
▪ Protein in urine
▪ Drug in urine etc..
Biochemical instruments
Microbiology department :
▪ Is the study of microorganisms , medical
laboratory scientists that work in this
department identifies bacteria , viruses ,
parasites or fungus , and also tell the physician
which antibiotics use to kill the bacteria that
found.
▪ Subareas of microbiology
▪ Virology
▪ Bacteriology
▪ Mycology
Microbiology instruments and tests :
▪ Autoclave
▪ Biosafety cabinet
▪ Petri dishes
▪ inoculation loops and
needles
▪ Coulter counter
▪ Shaker incubator
▪ Oven and Water bath
▪ GSE
▪ H.pylori Ag and Ab
▪ Seminal fluid analysis
▪ Gram staining
▪ Culture (urine ,blood ,
stool , CSF , bacteria , and
fungus )
▪ Detection of viral
infection by ELISA
Hematology :
▪ Is the study of blood components , in this
department the technician will counts cells in
blood including RBCs , WBCs and platelets .
And identifies the morphology of this cells to
detect diseases .
Instruments and tests:
▪ Coulter
▪ Hematology analyzer
▪ Coagulation analyzer
▪ Hemocytometer
chamber
▪ Microscope
▪ Hematocrit centrifuge
▪ CBC , CBP
▪ ESR
▪ WBC , RBC , reticulocytes
and platelet count
▪ Blood group
▪ DLC
▪ BT , CT
▪ Vit B12 ,Vit K ,folate, iron ,
ferritin
▪ Fibrinogen
▪ PT , PTT , INR
▪ Hb
Clinical Pathology :
Is the branch of medical science that studies the
cause, nature and effects of diseases .
It subdivided to
▪ histopathology which is the microscope examination
of tissue affected by disease
▪ cytology the examination and identifying
morphology of cells
Tissue processor
Molecular diagnostics :
▪ The molecular diagnostic laboratory is responsible
for the development and performance of molecular
diagnostic tests for nucleic acid targets found in a
variety of settings in medicine
▪ Molecular diagnosis is the most appropriate for
infectious agents that are difficult to detect identify
or test for susceptibility in a timely fashion with
conventional methods
molecular tests are done by PCR
and RT.PCR
Thermo cycler PCR
RT.PCR
agarose gel electrophoresis used to
visualize PCR results
▪ The results of a PCR reaction are usually
visualized (made visible) using gel
electrophoresis. Gel electrophoresis is a
technique in which fragments of DNA are
pulled through a gel matrix by an electric
current, and it separates DNA fragments
according to size.
Thank you

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Medical laboratory

  • 1. MEDICAL LABORATORY Aveen rasul clinical biochemistry department
  • 2. what’s medical laboratory? ▪ Medical laboratory is a place where tests are done on clinical specimens and samples in order to get information about the health of a patient as pertaining to the diagnosis , treatment , and prevention of disease .
  • 3. Job opportunities : ▪ Generalist : a medical laboratory scientist that works in all laboratory departments . ▪ Specialist : a medical laboratory scientist that has had three years experience in a particular department in addition to passing a specialist exam . This professional will work only in this department . ▪ Supervisor : a medical laboratory scientist that has risen in the ranks of the laboratory to become the head of particular department .
  • 4. Basic equipments used in a medical laboratory :
  • 5. Departments in medical laboratory : ▪ Clinical chemistry ( Biochemistry ) ▪ Hematology ▪ Pathology ▪ Microbiology ▪ immunology ▪ Blood banking ▪ Molecular technique
  • 6. Clinical biochemistry department : ▪ Biochemistry performs most chemical analysis includes hormones , enzyme , electrolytes , and drug levels in your blood stream , urine or other body fluids . ▪ It’s Subdevided ito : ▪ Biochemical analysis ▪ immunochemistry ▪ Toxicology
  • 7. Some Tests and instruments in biochemistry laboratory : ▪ Spectrophotometer ▪ Biochemistry analyzer ▪ ELISA ▪ Blood gas analyzer ▪ Urine analyzer ▪ Ph meter ▪ Electrolyte analyzer ▪ Gel electrophoresis instrument.. ▪ Renal function test (RFT) ▪ Liver function test (LFT) ▪ HbA1c , glucose , GTT ▪ Electrolyte ▪ Amylase and lipase ▪ Tumor markers ▪ Vitamin D ▪ Arterial blood gases ▪ Lipid profile ▪ Hormone ▪ Thyroid functions ▪ Protein in urine ▪ Drug in urine etc..
  • 9. Microbiology department : ▪ Is the study of microorganisms , medical laboratory scientists that work in this department identifies bacteria , viruses , parasites or fungus , and also tell the physician which antibiotics use to kill the bacteria that found. ▪ Subareas of microbiology ▪ Virology ▪ Bacteriology ▪ Mycology
  • 10. Microbiology instruments and tests : ▪ Autoclave ▪ Biosafety cabinet ▪ Petri dishes ▪ inoculation loops and needles ▪ Coulter counter ▪ Shaker incubator ▪ Oven and Water bath ▪ GSE ▪ H.pylori Ag and Ab ▪ Seminal fluid analysis ▪ Gram staining ▪ Culture (urine ,blood , stool , CSF , bacteria , and fungus ) ▪ Detection of viral infection by ELISA
  • 11.
  • 12. Hematology : ▪ Is the study of blood components , in this department the technician will counts cells in blood including RBCs , WBCs and platelets . And identifies the morphology of this cells to detect diseases .
  • 13. Instruments and tests: ▪ Coulter ▪ Hematology analyzer ▪ Coagulation analyzer ▪ Hemocytometer chamber ▪ Microscope ▪ Hematocrit centrifuge ▪ CBC , CBP ▪ ESR ▪ WBC , RBC , reticulocytes and platelet count ▪ Blood group ▪ DLC ▪ BT , CT ▪ Vit B12 ,Vit K ,folate, iron , ferritin ▪ Fibrinogen ▪ PT , PTT , INR ▪ Hb
  • 14.
  • 15. Clinical Pathology : Is the branch of medical science that studies the cause, nature and effects of diseases . It subdivided to ▪ histopathology which is the microscope examination of tissue affected by disease ▪ cytology the examination and identifying morphology of cells
  • 17. Molecular diagnostics : ▪ The molecular diagnostic laboratory is responsible for the development and performance of molecular diagnostic tests for nucleic acid targets found in a variety of settings in medicine ▪ Molecular diagnosis is the most appropriate for infectious agents that are difficult to detect identify or test for susceptibility in a timely fashion with conventional methods
  • 18. molecular tests are done by PCR and RT.PCR Thermo cycler PCR RT.PCR
  • 19. agarose gel electrophoresis used to visualize PCR results ▪ The results of a PCR reaction are usually visualized (made visible) using gel electrophoresis. Gel electrophoresis is a technique in which fragments of DNA are pulled through a gel matrix by an electric current, and it separates DNA fragments according to size.
  • 20.