The document describes the scientific method, which consists of 7 steps: 1) make observations, 2) state the problem, 3) collect preliminary data, 4) formulate a hypothesis, 5) test the hypothesis through experiments that have independent and dependent variables as well as control and experimental groups, 6) collect and analyze data, and 7) draw a conclusion. It provides examples of applying these steps, such as designing an experiment to test the hypothesis that plants can grow without direct sunlight.
This is the second of a two part lesson on the scientific method. The earlier lesson was all about variables and this one focuses more on the procedures of the scientific method, at about the 5th and 6th grade level.
This is the second of a two part lesson on the scientific method. The earlier lesson was all about variables and this one focuses more on the procedures of the scientific method, at about the 5th and 6th grade level.
First Quarter-Scientific Method Powerpoint Presentation
Content Standard-Scientific ways of acquiring knowledge and solving problems
Performance Standard
perform in groups
in guided
investigations involving community- based problems using locally available materials
Most Essential Learning
Competencies
-Describe the components
of a scientific investigation (Week 1) S7MTIa-
1
OBJECTIVES
Describe the components of the scientific method
Follow the steps of the scientific method and perform experiments using it.
SCIENTIFIC METHOD
a systematic process of empirical investigation
It is the key to unlock the bodies of knowledge by helping the researcher in organizing his or her thoughts and procedures and making him or her confident of the findings from the expirements
COMPONENTS OF A SCIENTIFIC INVESTIGATION
1. Statement of the Problem
-You should have existing knowledge of the problem
This part answers the following questions:
What questions do you have about your topic?
What do you want to know?
EXAMPLE
How does fertilizer affect the growth of plants?
2. Formulation of Hypothesis
Hypothesis-simple statement that presents the possible solution to the problem. It can be tested, and it is based on knowledge and research.
Hypothesis may be stated in two ways:
a. Null Hypothesis (Ho)-states that no relationship between variables
Example: The fertilizer DOES NOT affect the growth of plants
b. Alternative hypothesis (Ha)-states a relationship between variables
Example: There is significant relationship between the growth of plants and the use of fertilizer
Ha2 : There is a negative relationship between A and B
(Less A is involved, the better B)
Ha3 There is a positive relationship between A and B.
(More B is involved, the better A)
3. Testing Hypothesis and Gathering of Data
Experiments-a set of manipulations or specific observations of nature, and it is considered the most important part of the scientific method.
Three Types of Experiments
1. Controlled Experiment-the observer tests the hypothesis by looking for changes brought by alteration to a variable
Variable- a characteristic, number, or quantity that increases or decreases over time or takes different values in different situations.
a. Controlled variables-variables that are kept constant.
b. Independent variables-factors that you change or alter during the experiment.
c. Dependent variables-variables that you observe. and they are considered the response to an independent variable
2. Natural experiments or quasi-experiments-Here, the observer does not manipulate any variable but simply collects all the possible data to determine the factors affecting a particular phenomenon.
3. Field experiment-named to draw a contrast with laboratory experiments. It examines the real world using scientific method.
Example: Political sciences, economics, and psychology
Prediction
a forecast of future events based on past observations.
Example: The plants will grow faster and strong
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
2. Learning Competency
Describe the components of a scientific investigation
(S7MT-Ia-1).
At the end of this lesson, the given DepEd learning
competency should be met by the students.
3. Learning Objectives
At the end of this lesson, the learner should be able to
do the following:
● Describe the steps of the scientific method.
● Apply the steps of the scientific method to different
scenarios.
4. Have you ever
been stuck in
a difficult
decision ---
such as
deciding
which road to
take?
5. Did you form a
guess from your
observations and
decided to test it
by taking one of
the two roads?
If yes, then you just
performed a
simplified process
of what we call the
scientific method.
6. Try it!
Warm-Up
Can you solve these
Science riddles?
Fact5. March 30, 2017.
‘5 Science Riddles That
Will Blow Your Mind.’
https://www.youtube.com
/watch?v=HBWIVTrLO6E.
Parents Know Best
7. Learn about It
It is a series of organized steps that are taken to answer a
question of solve a problem.
Scientific Method
8. Learn about It
● Observation is a way of acquiring information using the five
senses
● There are two types of observations:
− Qualitative observations are observations from the five
senses.
− Quantitative observations are numerical values
obtained from measuring tools.
1. Make observations.
9. Learn about It
● In this step, you need to define the
problem that you want to solve or
know.
● The problem is usually in the form of
a question.
2. State the problem.
10. Learn about It
● Once you know the problem that
you want to answer, you should
read about it.
● Analyze the facts and data that
you collect, and look for trends
and patterns.
3. Collect preliminary data.
11. Learn about It
● After collecting necessary information, a
possible answer derived from the
collected information is formulated.
● A hypothesis is an educated guess that
may lead to a possible solution to the
problem.
● Commonly, it is stated as a cause-and-
effect relationship, making it testable.
4. Formulate a hypothesis.
12. Learn about It
● An experiment is performed to validate the hypothesis.
− A controlled experiment is designed to test the effect of
a change in a condition. These conditions or factors that
affect the outcome of an experiment is called a variable.
5. Test the hypothesis.
13. Learn about It
● There are two types of variables:
− The independent variable is the value that is being
changed or manipulated.
− The dependent variable is the factor that changes
relying on the change in the independent variable.
5. Test the hypothesis.
14. Learn about It
● If the dependent variable increases as the independent
variable increases, or the dependent variable decreases as
the independent variable decreases, they are said to be
directly proportional.
● If the dependent variable decreases as the independent
variable increases (or vice versa), they are said to be
inversely proportional.
5. Test the hypothesis.
15. Learn about It
● It is also usual for experiments to have two setups.
● The experimental group is the setup that all possible
variables are held constant except for the variable that is
manipulated in the experiment (independent variable).
● A control group is not exposed to any conditions of the
experiment and all other variables are held constant.
5. Test the hypothesis.
16. Learn about It
● The data from the experiment will either support or oppose
your hypothesis.
● Data gathered may be qualitative or quantitative. They are
usually presented in tables.
6. Collect and analyze data.
17. Learn about It
● Does the data support or invalidate the
hypothesis?
● If the data supports the hypothesis, then
the problem is solved.
● The study should then be shared to
other scientists to spread the
information.
7. Draw a conclusion.
18. Learn about It
● Other scientists may also work on your
hypothesis to further support your study.
● If the data otherwise opposes the
hypothesis, this does not mean that the
experiment is a failure.
● If this happens, a new hypothesis can be
made and another experiment may be
designed.
7. Draw a conclusion.
19. Try It!
You noticed that one plant was growing under
direct sunlight while another was hidden under
the shadow of the walls but both are growing
healthily. You hypothesize that direct sunlight is
not needed for plants to grow.
Design an experiment to test this hypothesis
and identify the independent and dependent
variable, and the control and experimental
group.
20. Try It! (Answer)
In the experiment, the following steps should be done:
● Gather two plants of the same kind, of the same age and size.
● Keep one away from direct sunlight. (experimental group)
● Keep one outside where it is exposed to sunlight. (control
group)
● The height of the two plants from the soil to the highest part
of the plant can be measured day for two to three weeks.
The independent variable is direct sunlight while the dependent
variable is the height of the plant.
21. Key Points
● The scientific method is a series of organized steps that
are taken to answer a question or solve a problem. The
steps of the scientific method are the following:
○ Make observations.
○ State the problem.
○ Collect preliminary data.
○ Formulate a hypothesis.
○ Test the hypothesis.
○ Collect and analyze data.
○ Draw a conclusion.
22. Key Points
● A hypothesis is an educated guess that may lead to a
possible solution to the problem.
● An experiment is performed to validate the hypothesis.
23. Check Your Understanding
During the ancient times, the Egyptians believed that
frogs are produced from mud. They had this belief
because every spring, many frogs appear in the mud near
the Nile River.
1. Given this scenario, use every
step of the scientific method
to accept or reject the
hypothesis on the origin of
frogs.
24. Check Your Understanding
During the ancient times, the Egyptians believed that
frogs are produced from mud. They had this belief
because every spring, many frogs appear in the mud near
the Nile River.
2. Identify the independent variable
and dependent variable.
2. Which is the experimental group
and which is the control group in
your experiment?
25. Bibliography
Boundless. “The Scientific Method.” Lumen Learning. Accessed January 31, 2022.
https://courses.lumenlearning.com/boundless-psychology/chapter/the-scientific-method/.
Bradford, Alina, and Ashley Hamer. “Science and the Scientific Method: Definitions and Examples.”
LiveScience. Purch, January 17, 2022. https://www.livescience.com/20896-science-scientific-
method.html.
Britannica, T. Editors of Encyclopaedia. "scientific method." Encyclopedia Britannica, October 15, 2021.
https://www.britannica.com/science/scientific-method.